TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QCM3d10.yg.2.1
(683 letters)
Database: Populus_nucl_with_EST.fasta
369,679 sequences; 203,408,664 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CA924040.1|CA924040 MTU7CL.P1.A07 Aspen leaf cDNA Librar... 279 2e-091
gb|DN500621.1|DN500621 UL69F06.5pR Populus cold stressed le... 297 5e-080
gb|CX171278.1|CX171278 G09_69-19_13.ab1 leaf inoculated wit... 129 1e-029
gb|CX174846.1|CX174846 B10_69-89_04.ab1 leaf inoculated wit... 129 1e-029
>gb|CA924040.1|CA924040 MTU7CL.P1.A07 Aspen leaf cDNA Library Populus tremuloides cDNA,
mRNA sequence
Length = 700
Score = 279 bits (604), Expect(2) = 2e-091
Identities = 117/145 (80%), Positives = 127/145 (87%)
Frame = +1 / -3
Query: 202 IWADIMRFFSNPRKPMILALARPDPKKNITTLVKAFGEHRELRNLANLTLIMGNRDVIDE 381
IW +IMRFF+N KPMILALARPDPKKNITTLV AFGE R LR LANLTLIMGNRD IDE
Sbjct: 437 IWIEIMRFFTNSHKPMILALARPDPKKNITTLVTAFGECRPLRELANLTLIMGNRDGIDE 258
Query: 382 MSSTNAAVLTSALKLIDKYDLYGQVAYPKHHKQSEVPDIYRLAARTKGVFINCALVEPFG 561
MSST+++VL S LKLIDKYDLYGQVAYPKHHKQS+VPDIYRLAA+TKGVFIN A +EPFG
Sbjct: 257 MSSTSSSVLLSVLKLIDKYDLYGQVAYPKHHKQSDVPDIYRLAAKTKGVFINPAFIEPFG 78
Query: 562 LTLIEXXAYGLPMXXTRNGGPVXIH 636
LTLIE A+GLPM T+NG PV IH
Sbjct: 77 LTLIEAAAHGLPMVATKNGSPVDIH 3
Score = 184 bits (397), Expect(2) = 2e-053
Identities = 81/120 (67%), Positives = 92/120 (76%)
Frame = -1 / +1
Query: 638 RCMXTGPPFRVXXMGRPYAXXSIKVSPNGSTNAQLIKTPFVLAAKR*ISGTSDCLWCLGY 459
RCM TG PF V +G P A SIKVSPNGS A L+KTPFV AA R*+SGTSDCL CLGY
Sbjct: 1 RCMSTGLPFLVATIGNP*AAASIKVSPNGSMKAGLMKTPFVFAASR*MSGTSDCL*CLGY 180
Query: 458 ATCPYRSYLSINLSAEVKTAAFVLDISSMTSRFPMISVRFAKFLSSRCSPNALTRVVIFF 279
ATCPYRSYLSI+LS E +T VLDISS+ SR P+I VR A L+ R SPNA+T+VV+FF
Sbjct: 181 ATCPYRSYLSISLSTERRTDELVLDISSIPSRLPIIRVRLASSLNGRHSPNAVTKVVMFF 360
Score = 134 bits (287), Expect(2) = 1e-038
Identities = 67/158 (42%), Positives = 95/158 (60%)
Frame = -3 / +3
Query: 636 MYVXRPTISGXXHG*TICXXLNQGESKWFNQCTIDKNSFCSRS*TINIRNFRLLMVLGVC 457
+YV +I G H ++ LN +SKW N+ ID+N+ C R *+IN+RN RLL++ VC
Sbjct: 3 VYVYWASIFGGNHRQSMSCCLN*SKSKWLNESRIDENTLCLRC*SINVRNIRLLVMFRVC 182
Query: 456 HLSI*IIFIN*LEC*SQNSCICA*HFINDITIPHDQCKIC*ISQFTMFTKCFD*SSDILL 277
+L I II +N L+ ++ CA HFIN I+I H+ K+ +SQ+ F+KC D S D+L
Sbjct: 183 NLPIQIILVNKLKHRKKDR*TCAGHFINSISIAHN*SKVSKLSQWPTFSKCCDQSCDVLF 362
Query: 276 RIRTSKSKNHWLPGV*EEAHYISPNRWIW*T*AISISF 163
I + K K H L + +EAH + P RWI I ISF
Sbjct: 363 GIWSCKGKYHRLVRIGKEAHDLYPYRWIRRRGMIFISF 476
Score = 77.1 bits (162), Expect(2) = 2e-091
Identities = 32/48 (66%), Positives = 35/48 (72%)
Frame = +1 / -3
Query: 10 DGFDLTMARKLRARIRRGVSCFGRXXXXMIAIPPGMEFSHIAPHDVDL 153
DGFD + RKLRARIRR VSC+GR M IPPGMEF HI P D D+
Sbjct: 632 DGFDPILERKLRARIRRNVSCYGRFMXRMAIIPPGMEFHHIVPQDGDM 489
Score = 45.5 bits (93), Expect(2) = 1e-038
Identities = 24/43 (55%), Positives = 29/43 (67%)
Frame = -1 / +1
Query: 137 GAI*LNSMPGGIAIIXXXXRPKQLTPRLILALSFRAIVRSKPS 9
G +* NS+PGGI + P QLT RLILA S R+ + SKPS
Sbjct: 505 GTM**NSIPGGIIAMRXMNLP*QLTLRLILARSLRSSIGSKPS 633
Score = 44.6 bits (91), Expect(2) = 2e-053
Identities = 20/53 (37%), Positives = 30/53 (56%)
Frame = -3 / +3
Query: 165 FLTVEVNIMWCYMTKLHARRDCNHXXXXTTKAAHTTPYSCSEFPGHS*IKTII 7
+ + + I+ + KLH+RR+ +H + H TP SCS+F IKTII
Sbjct: 477 YFAIHITILGNNVMKLHSRRNYSHAXHEPSVTTHVTPDSCSQFTLKYRIKTII 635
>gb|DN500621.1|DN500621 UL69F06.5pR Populus cold stressed leaves cDNA library Populus
tremula x Populus tremuloides cDNA clone UL69F06 5',
mRNA sequence
Length = 789
Score = 297 bits (643), Expect = 5e-080
Identities = 124/152 (81%), Positives = 135/152 (88%)
Frame = +1 / +1
Query: 202 IWADIMRFFSNPRKPMILALARPDPKKNITTLVKAFGEHRELRNLANLTLIMGNRDVIDE 381
IW +IMRFF+N KPMILALARPDPKKNITTLV AFGE R LR LANLTLIMGNRD IDE
Sbjct: 40 IWIEIMRFFTNSHKPMILALARPDPKKNITTLVTAFGECRPLRELANLTLIMGNRDGIDE 219
Query: 382 MSSTNAAVLTSALKLIDKYDLYGQVAYPKHHKQSEVPDIYRLAARTKGVFINCALVEPFG 561
MSST+++VL S LKLIDKYDLYGQVAYPKHHKQS+VPDIYRLAA+TKGVFIN A +EPFG
Sbjct: 220 MSSTSSSVLLSVLKLIDKYDLYGQVAYPKHHKQSDVPDIYRLAAKTKGVFINPAFIEPFG 399
Query: 562 LTLIEXXAYGLPMXXTRNGGPVXIHRVLDNGI 657
LTLIE A+GLPM T+NGGPV IHRVLDNG+
Sbjct: 400 LTLIEAAAHGLPMVATKNGGPVDIHRVLDNGL 495
Score = 197 bits (425), Expect = 6e-050
Identities = 86/125 (68%), Positives = 97/125 (77%)
Frame = -1 / -2
Query: 653 PLSRTRCMXTGPPFRVXXMGRPYAXXSIKVSPNGSTNAQLIKTPFVLAAKR*ISGTSDCL 474
PLS TRCM TGPPF V +G P A SIKVSPNGS A L+KTPFV AA R*+SGTSDCL
Sbjct: 491 PLSSTRCMSTGPPFFVATIGNP*AAASIKVSPNGSMKAGLMKTPFVFAASR*MSGTSDCL 312
Query: 473 WCLGYATCPYRSYLSINLSAEVKTAAFVLDISSMTSRFPMISVRFAKFLSSRCSPNALTR 294
CLGYATCPYRSYLSI+LS E +T VLDISS+ SR P+I VR A L+ R SPNA+T+
Sbjct: 311 *CLGYATCPYRSYLSISLSTERRTDELVLDISSIPSRLPIIRVRLASSLNGRHSPNAVTK 132
Query: 293 VVIFF 279
VV+FF
Sbjct: 131 VVMFF 117
Score = 138 bits (295), Expect = 5e-032
Identities = 68/164 (41%), Positives = 99/164 (60%)
Frame = -3 / -1
Query: 654 SIIKNPMYVXRPTISGXXHG*TICXXLNQGESKWFNQCTIDKNSFCSRS*TINIRNFRLL 475
+I+K P+YV +I H ++ LN +SKW N+ ID+N+ C R *+IN+RN RLL
Sbjct: 492 TIVKYPVYVYWASIFCGNHRQSMSCCLN*SKSKWLNESRIDENTLCLRC*SINVRNIRLL 313
Query: 474 MVLGVCHLSI*IIFIN*LEC*SQNSCICA*HFINDITIPHDQCKIC*ISQFTMFTKCFD* 295
++ VC+L I II +N L+ ++ CA HFIN I+I H+ K+ +SQ+ F+KC D
Sbjct: 312 VMFRVCNLPIQIILVNKLKHRKKDR*TCAGHFINSISIAHN*SKVSKLSQWPTFSKCCDQ 133
Query: 294 SSDILLRIRTSKSKNHWLPGV*EEAHYISPNRWIW*T*AISISF 163
S D+L I + K K H L + +EAH + P RWI + ISF
Sbjct: 132 SCDVLFGIWSCKGKYHRLVRIGKEAHDLYPYRWIRRRGMVFISF 1
>gb|CX171278.1|CX171278 G09_69-19_13.ab1 leaf inoculated with Marssonia pathogen of Populus
deltoides Populus deltoides cDNA, mRNA sequence
Length = 698
Score = 129 bits (277), Expect = 1e-029
Identities = 51/64 (79%), Positives = 57/64 (89%)
Frame = +1 / +3
Query: 466 KHHKQSEVPDIYRLAARTKGVFINCALVEPFGLTLIEXXAYGLPMXXTRNGGPVXIHRVL 645
KHHKQS+VPDIYRLAA+TKGVFIN A +EPFGLTLIE A+GLPM T+NGGPV IHRVL
Sbjct: 51 KHHKQSDVPDIYRLAAKTKGVFINPAFIEPFGLTLIEAAAHGLPMVATKNGGPVDIHRVL 230
Query: 646 DNGI 657
DNG+
Sbjct: 231 DNGL 242
Score = 103 bits (220), Expect = 1e-021
Identities = 44/62 (70%), Positives = 47/62 (75%)
Frame = -1 / -2
Query: 653 PLSRTRCMXTGPPFRVXXMGRPYAXXSIKVSPNGSTNAQLIKTPFVLAAKR*ISGTSDCL 474
PLS TRCM TGPPF V +G P A SIKVSPNGS A L+KTPFV AA R*+SGTSDCL
Sbjct: 238 PLSSTRCMSTGPPFLVATIGNP*AAASIKVSPNGSMKAGLMKTPFVFAASR*MSGTSDCL 59
Query: 473 WC 468
C
Sbjct: 58 *C 53
>gb|CX174846.1|CX174846 B10_69-89_04.ab1 leaf inoculated with Marssonia pathogen of Populus
deltoides Populus deltoides cDNA, mRNA sequence
Length = 680
Score = 129 bits (277), Expect = 1e-029
Identities = 51/64 (79%), Positives = 57/64 (89%)
Frame = +1 / +3
Query: 466 KHHKQSEVPDIYRLAARTKGVFINCALVEPFGLTLIEXXAYGLPMXXTRNGGPVXIHRVL 645
KHHKQS+VPDIYRLAA+TKGVFIN A +EPFGLTLIE A+GLPM T+NGGPV IHRVL
Sbjct: 33 KHHKQSDVPDIYRLAAKTKGVFINPAFIEPFGLTLIEAAAHGLPMVATKNGGPVDIHRVL 212
Query: 646 DNGI 657
DNG+
Sbjct: 213 DNGL 224
Score = 103 bits (220), Expect = 1e-021
Identities = 44/62 (70%), Positives = 47/62 (75%)
Frame = -1 / -2
Query: 653 PLSRTRCMXTGPPFRVXXMGRPYAXXSIKVSPNGSTNAQLIKTPFVLAAKR*ISGTSDCL 474
PLS TRCM TGPPF V +G P A SIKVSPNGS A L+KTPFV AA R*+SGTSDCL
Sbjct: 220 PLSSTRCMSTGPPFLVATIGNP*AAASIKVSPNGSMKAGLMKTPFVFAASR*MSGTSDCL 41
Query: 473 WC 468
C
Sbjct: 40 *C 35
Database: Populus_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:49 PM
Number of letters in database: 203,408,664
Number of sequences in database: 369,679
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 294,896,506
Number of Sequences: 369679
Number of extensions: 3664353
Number of successful extensions: 26948
Number of sequences better than 1.0e-020: 5
length of database: 67,802,888
effective HSP length: 51
effective length of database: 48,949,259
effective search space used: 8615069584
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)