TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QCH30c02.yg.2.1
(722 letters)
Database: Populus_nucl_with_EST.fasta
369,679 sequences; 203,408,664 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|DT500846.1|DT500846 PX0019.BR_H22 PT-X-FL-A-1 Populus tr... 101 2e-023
>gb|DT500846.1|DT500846 PX0019.BR_H22 PT-X-FL-A-1 Populus trichocarpa cDNA clone PX0019_H22
5', mRNA sequence
Length = 645
Score = 101 bits (216), Expect(2) = 2e-023
Identities = 39/75 (52%), Positives = 55/75 (73%)
Frame = -2 / +1
Query: 295 VTAFGEDTKARMATEAVFTIATVYGDHIRSGWRNIIDCILRLHKIGLLPGPLTGDTADDQ 116
V AFG+D +A MAT VFTIA YGD+IR+GWRNI+DC+LRL+K+GLLP + D ++
Sbjct: 4 VLAFGDDARAMMATVTVFTIANRYGDYIRTGWRNILDCVLRLYKLGLLPARVASDATEES 183
Query: 115 DSSSKSLPGKLASSA 71
+ S+ + GK S++
Sbjct: 184 ELSADPVHGKTISNS 228
Score = 27.2 bits (53), Expect(2) = 2e-023
Identities = 11/18 (61%), Positives = 13/18 (72%)
Frame = -2 / +1
Query: 55 ISTSRKTYGLMGRFSQLL 2
+ T R+ GLMGRFSQ L
Sbjct: 253 METPRRYTGLMGRFSQFL 306
Database: Populus_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:49 PM
Number of letters in database: 203,408,664
Number of sequences in database: 369,679
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 279,351,483
Number of Sequences: 369679
Number of extensions: 3528778
Number of successful extensions: 33532
Number of sequences better than 1.0e-020: 1
length of database: 67,802,888
effective HSP length: 52
effective length of database: 48,579,580
effective search space used: 9132961040
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)