TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QCH17d04.yg.2.1
(637 letters)
Database: Populus_nucl_with_EST.fasta
369,679 sequences; 203,408,664 total letters
Score E
Sequences producing significant alignments: (bits) Value
emb|AJ891040.1| Populus tremula x Populus alba mRNA for alp... 369 e-102
gb|DT526453.1|DT526453 WS02048.C21_B09 PTxN-IB-N-A-11 Popul... 118 4e-026
gb|CV272375.1|CV272375 WS0157.B21_H14 PTxN-IB-A-6 Populus t... 102 2e-021
>emb|AJ891040.1| Populus tremula x Populus alba mRNA for alpha 1,3 fucosyltransferase
(poFUT2 gene), clone INRA 717 1B4
Length = 1569
Score = 369 bits (800), Expect = e-102
Identities = 145/212 (68%), Positives = 171/212 (80%)
Frame = -1 / +1
Query: 637 RYRFSXXFEXXXEXDYXXEKFFQXXVXGSIPVVVGAPNIQEFXPGEGAILHIXXLDDVAS 458
RY+FS FE E DY EKFFQ V G+IPVVVGAPNIQ+F P +ILHI L+DV S
Sbjct: 847 RYKFSLAFENSNEEDYVTEKFFQSLVAGTIPVVVGAPNIQDFAPAPNSILHIRKLEDVDS 1026
Query: 457 VAKTMKNIASNPDAFNQSLRWKYDGPSDSFKALIDMAAVHSSCRLCIHIATKIHLKEERT 278
+AKTMK + NPDA+NQSLRWKY+GPSDSFKAL+DMAAVHSSCRLCIH+AT I KEE +
Sbjct: 1027 IAKTMKYLGENPDAYNQSLRWKYEGPSDSFKALVDMAAVHSSCRLCIHLATMIREKEENS 1206
Query: 277 PKFTNRPCSCSTKKGTIYHLFIRERGRFKSESIYMRSGQLTLGALESAVLGKFRSLNHVP 98
P F RPC C+ T+YHL++RERGRF+ ESI++RSG LT+ ALE+AVL KF+SL HVP
Sbjct: 1207 PGFKRRPCRCTKDLETVYHLYVRERGRFQMESIFLRSGNLTVNALEAAVLKKFKSLKHVP 1386
Query: 97 VWKDERPPSIRGGDDLKLYRIYPVGLTQRQAL 2
+WK ERP SIRGGDD K+YR+YPVG+TQRQAL
Sbjct: 1387 IWKQERPESIRGGDDFKVYRVYPVGMTQRQAL 1482
Score = 198 bits (426), Expect = 4e-050
Identities = 109/207 (52%), Positives = 121/207 (58%)
Frame = +3 / -2
Query: 15 CVRPTG*ILYNFRXXXXXXXXXXXXFHTGTWLRDLNLPSTADSKAPRVNWPDLM*MLSDL 194
CV PTG L + F GT +DLN STA S+A V PDL M S
Sbjct: 1469 CVMPTGYTL*TLKSSPPLMLSGLSCFQMGTCFKDLNFLSTAASRAFTVRLPDLKKMDSI* 1290
Query: 195 NRPLSRINKW*IVPFLVEQLQGRFVNFGVLSSFKWILVAICIQRRQDE*TAAMSIRALKE 374
N PLS K V + LQGR +N G+ SSF I+VA +QRR DE TAA+S ALKE
Sbjct: 1289 NLPLSLTYK*YTVSKSLVHLQGRLLNPGLFSSFSRIIVAR*MQRRHDECTAAISTSALKE 1110
Query: 375 SDGPSYFHLKD*LKASGFEAIFFIVLATEATSSSXLICNIAPSPGXNS*IFGAPTTTGIE 554
SDGPSYFHL D*L ASGF +FIV A E+TSSS LIC I G S*IFGAPTTTG+
Sbjct: 1109 SDGPSYFHLND*L*ASGFSPRYFIVFAIESTSSSFLICKIELGAGAKS*IFGAPTTTGMV 930
Query: 555 PXTXX*KNFSXX*SXSXXXSNXXLNL* 635
P T KNFS *S S S LNL*
Sbjct: 929 PATRDWKNFSVT*SSSLEFSKAKLNL* 849
Score = 123 bits (263), Expect = 1e-027
Identities = 78/211 (36%), Positives = 107/211 (50%)
Frame = +2 / -1
Query: 2 QSLTLR*TDWVNSV*FQVIPTSNARWPFILPYRNVVEGPKFTEHCGFQGSQS*LA*SHVN 181
QSL L V SV F+++ + A F+LP ++++ K EHC S*+A N
Sbjct: 1482 QSLPLCHAYRVYSVNFKIVSSPYAFRSFLLPDGHMLQRLKLLEHCSL*SIYS*IARPQEN 1303
Query: 182 AL*LKPPSLSDK*VVNCSLFGGTATRTICKFWSPFLL*MDLGSNMYTKTTR*MNRCHVNK 361
L LK SLS *+++ G + T K W+ FL + S + KT *+ CH+ +
Sbjct: 1302 GLHLKSSSLSYI*MIHGL*VFGASAGTPLKPWAIFLFLPNHSSKVNAKTA**VYCCHIYQ 1123
Query: 362 SFERIGWTIILPPQRLIEGIRV*SNIFHCLSN*SNIIKLXNM*YCAFSRXKLLNIWSXXX 541
ERI W ++ PP LI GIR+ S +FH L N* NI L +M* R K+LNIWS
Sbjct: 1122 CLERIRWALVFPP**LIVGIRIFSKVFHSLRN*INIF*LPDM*N*VRCRSKILNIWSTNY 943
Query: 542 XXXXXXXXXLKKLFXXIILLXXXLKXXAESV 634
L++ F IILL LK A+ V
Sbjct: 942 HRYGSSNKRLEEFFCDIILLIGILKSQAKFV 850
>gb|DT526453.1|DT526453 WS02048.C21_B09 PTxN-IB-N-A-11 Populus trichocarpa x Populus nigra
cDNA clone WS02048_B09 3', mRNA sequence
Length = 677
Score = 118 bits (252), Expect = 4e-026
Identities = 46/62 (74%), Positives = 55/62 (88%)
Frame = -1 / -3
Query: 187 ESIYMRSGQLTLGALESAVLGKFRSLNHVPVWKDERPPSIRGGDDLKLYRIYPVGLTQRQ 8
ESI++RSG LT+ ALE+AVL KF+SL HVP+WK ERP SIRGGDD K+YR+YPVG+TQRQ
Sbjct: 669 ESIFLRSGNLTVKALEAAVLKKFKSLKHVPIWKQERPESIRGGDDFKVYRVYPVGMTQRQ 490
Query: 7 AL 2
AL
Sbjct: 489 AL 484
>gb|CV272375.1|CV272375 WS0157.B21_H14 PTxN-IB-A-6 Populus trichocarpa x Populus nigra cDNA
clone WS0157_H14 3', mRNA sequence
Length = 688
Score = 102 bits (217), Expect = 2e-021
Identities = 39/52 (75%), Positives = 46/52 (88%)
Frame = -1 / -3
Query: 157 TLGALESAVLGKFRSLNHVPVWKDERPPSIRGGDDLKLYRIYPVGLTQRQAL 2
T+ ALE+AVL KF+SL HVP+WK ERP SIRGGDD K+YR+YPVG+TQRQAL
Sbjct: 686 TVNALEAAVLKKFKSLKHVPIWKQERPESIRGGDDFKVYRVYPVGMTQRQAL 531
Database: Populus_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:49 PM
Number of letters in database: 203,408,664
Number of sequences in database: 369,679
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 280,869,759
Number of Sequences: 369679
Number of extensions: 3715509
Number of successful extensions: 34098
Number of sequences better than 1.0e-020: 3
length of database: 67,802,888
effective HSP length: 51
effective length of database: 48,949,259
effective search space used: 7831881440
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)