TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QCA13d08.yg.2.1
(511 letters)
Database: Populus_nucl_with_EST.fasta
369,679 sequences; 203,408,664 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN518516.1|CN518516 GQ0094.B3_D08 GQ009 Populus trichoca... 177 3e-044
gb|DT503237.1|DT503237 WS0135.BR_P04 PTxD-IL-FL-A-4 Populus... 123 8e-028
gb|DT474356.1|DT474356 WS01231.BR_K07 PT-GT-FL-A-3 Populus ... 101 3e-021
>gb|CN518516.1|CN518516 GQ0094.B3_D08 GQ009 Populus trichocarpa x Populus deltoides cDNA
clone GQ0094_D08 5', mRNA sequence
Length = 552
Score = 177 bits (382), Expect = 3e-044
Identities = 75/143 (52%), Positives = 98/143 (68%)
Frame = +1 / +1
Query: 82 IMTYYEAKMNTRLRNXXXXXXXXXXXXXXXXXDVATKGHGGLGVFVGVCIISAIFGTADA 261
I+TYYE+K++TR RN D+A+ G GG+G F+G+ I+ FG ADA
Sbjct: 4 ILTYYESKIDTRKRNLSGLVLFFLSSLLLLLLDLASSGKGGIGNFIGIGAIAGSFGVADA 183
Query: 262 NCQGALVGDLSLMCPEFIQSFMAGLAASGVLTSALRLVTKAAFESSKDGLRIGAILFFSI 441
QG +VGDL MCPEF+QS++AG+AASG L SALRL+TKAAF+ +GLR G ILF I
Sbjct: 184 VLQGGMVGDLFFMCPEFLQSYLAGIAASGFLISALRLLTKAAFDKFPNGLRKGVILFLVI 363
Query: 442 TCLFELVCLLLYTFVFGKLPIVK 510
+ FE +C+L+Y F+F KLPIVK
Sbjct: 364 SIFFEFLCILVYAFLFPKLPIVK 432
Score = 145 bits (311), Expect = 2e-034
Identities = 72/141 (51%), Positives = 86/141 (60%)
Frame = -3 / -2
Query: 509 FTMGSLPKTNVYSRRHTSSNRHVIEKNSIAPMRRPSFELSKAALVTNLKADVRTPDAASP 330
FT+GSL K N Y+R H +S + I KNSI P+RRP LS AA V++L+A++R PDAA P
Sbjct: 431 FTIGSLGKRNAYTRMHRNSKKMEITKNSITPLRRPLGNLSNAAFVSSLRAEIRKPDAAIP 252
Query: 329 AMKD*MNSGHIKERSPTSAP*QLASAVPNMALIMHTPTKTPSPPCPLVATSRIINAKEPR 150
A D* NSGHIK RSPT P*+ ASA P I P K P PP P A S+ + E +
Sbjct: 251 AR*D*RNSGHIKNRSPTIPP*RTASATPKEPAIAPIPMKFPIPPLPDEAKSKSRSNNELK 72
Query: 149 KKRENPARFLNLVFIFAS*YV 87
KK P R V I S*YV
Sbjct: 71 KKSTRPDRLRFRVSILDS*YV 9
>gb|DT503237.1|DT503237 WS0135.BR_P04 PTxD-IL-FL-A-4 Populus trichocarpa x Populus
deltoides cDNA clone WS0135_P04 5', mRNA sequence
Length = 908
Score = 123 bits (263), Expect = 8e-028
Identities = 51/98 (52%), Positives = 62/98 (63%)
Frame = +1 / +3
Query: 37 VLTLVYQPFAFGITLIMTYYEAKMNTRLRNXXXXXXXXXXXXXXXXXDVATKGHGGLGVF 216
VLTLVYQPFA G I+ Y EAK+NTR RN D+AT G GG+G F
Sbjct: 615 VLTLVYQPFAIGTMAILAYNEAKINTRKRNIAGYILFTASTLMLMVVDLATSGRGGVGPF 794
Query: 217 VGVCIISAIFGTADANCQGALVGDLSLMCPEFIQSFMA 330
+ +C I A FG ADA+ QG +VGD++ MCPEF+QSF A
Sbjct: 795 ICICAIVAAFGVADAHVQGGMVGDMAFMCPEFMQSFFA 908
>gb|DT474356.1|DT474356 WS01231.BR_K07 PT-GT-FL-A-3 Populus trichocarpa cDNA clone
WS01231_K07 5', mRNA sequence
Length = 944
Score = 101 bits (215), Expect = 3e-021
Identities = 42/97 (43%), Positives = 57/97 (58%)
Frame = +1 / +3
Query: 37 VLTLVYQPFAFGITLIMTYYEAKMNTRLRNXXXXXXXXXXXXXXXXXDVATKGHGGLGVF 216
+LTL+Y PFA I+TYYE+K++TR RN D+A+ G GG+G F
Sbjct: 594 LLTLIYMPFAVVSMAILTYYESKIDTRKRNLSGLVLFFLSSLLLLLLDLASSGKGGIGNF 773
Query: 217 VGVCIISAIFGTADANCQGALVGDLSLMCPEFIQSFM 327
+G+ I+ FG ADA QG +VGDL MCPEF+ +
Sbjct: 774 IGIGAIAGSFGVADALLQGGMVGDLFFMCPEFLSPIL 884
Database: Populus_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:49 PM
Number of letters in database: 203,408,664
Number of sequences in database: 369,679
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 268,913,133
Number of Sequences: 369679
Number of extensions: 4005583
Number of successful extensions: 90637
Number of sequences better than 1.0e-020: 4
length of database: 67,802,888
effective HSP length: 51
effective length of database: 48,949,259
effective search space used: 5776012562
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)