TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QBTB.006B01F020311.3.1
(609 letters)
Database: Populus_nucl_with_EST.fasta
369,679 sequences; 203,408,664 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|DT474803.1|DT474803 WS0125.BR_A19 PT-GT-FL-A-3 Populus t... 152 1e-046
gb|AC149484.1| Populus trichocarpa clone Pop1-69M19, comple... 92 1e-039
gb|DN501581.1|DN501581 V006H08.5pR Populus male catkins cDN... 147 6e-035
gb|AJ774822.1|AJ774822 AJ774822 Populus euphratica shoot 3-... 131 4e-030
>gb|DT474803.1|DT474803 WS0125.BR_A19 PT-GT-FL-A-3 Populus trichocarpa cDNA clone
WS0125_A19 5', mRNA sequence
Length = 585
Score = 152 bits (326), Expect(2) = 1e-046
Identities = 59/80 (73%), Positives = 67/80 (83%)
Frame = +1 / +3
Query: 175 RKIPDTFLGVFFEEMGHGGAGGIWAELVSNRGFEAGGPNTPSNIDPWLIVGDDSSVYVET 354
R IP+T G+FFEE+ H GAGGIWAELV+NRGFEAGG NTPSNI PW I+GD SS+ V T
Sbjct: 273 RPIPETLFGIFFEEINHAGAGGIWAELVNNRGFEAGGQNTPSNIAPWSIIGDQSSLIVST 452
Query: 355 DRSSCFSRNIVALRMEVLCN 414
DRSSCF RN VALRM+VLC+
Sbjct: 453 DRSSCFERNKVALRMDVLCD 512
Score = 81.1 bits (171), Expect(3) = 4e-024
Identities = 33/47 (70%), Positives = 38/47 (80%)
Frame = -2 / -3
Query: 314 QGSMFDGVLGPPASKPLLLTSSAHIPPAPPWPISSKNTPRNVSGIFR 174
QG++F+GV PPASKP L TSSAHIPPAP W ISSK P++VSGI R
Sbjct: 412 QGAIFEGVFWPPASKPRLFTSSAHIPPAPAWFISSKKIPKSVSGIGR 272
Score = 54.6 bits (113), Expect(2) = 1e-046
Identities = 19/20 (95%), Positives = 19/20 (95%)
Frame = +1 / +3
Query: 412 NDCPAGGVGIYNPGFWGMNI 471
N CPAGGVGIYNPGFWGMNI
Sbjct: 525 NICPAGGVGIYNPGFWGMNI 584
Score = 42.7 bits (87), Expect(3) = 4e-024
Identities = 16/20 (80%), Positives = 18/20 (90%)
Frame = -2 / -3
Query: 470 MFMPQNPGL*MPTPPAGQSL 411
+FMPQNPGL +PTPPAGQ L
Sbjct: 583 IFMPQNPGLYIPTPPAGQIL 524
Score = 24.4 bits (47), Expect(3) = 4e-024
Identities = 10/16 (62%), Positives = 12/16 (75%)
Frame = -2 / -3
Query: 410 QRTSILRATMFRLKHD 363
Q TSI RAT+FR K +
Sbjct: 508 QSTSIRRATLFRSKQE 461
>gb|AC149484.1| Populus trichocarpa clone Pop1-69M19, complete sequence
Length = 96930
Score = 92.1 bits (195), Expect(5) = 1e-039
Identities = 37/49 (75%), Positives = 40/49 (81%)
Frame = +1 / -1
Query: 268 GFEAGGPNTPSNIDPWLIVGDDSSVYVETDRSSCFSRNIVALRMEVLCN 414
GFEAGG NTPSNI PW I+GD SS+ V TDRSSCF RN VALRM VLC+
Sbjct: 72342 GFEAGGQNTPSNIAPWSIIGDQSSLTVSTDRSSCFERNKVALRMHVLCD 72196
Score = 50.0 bits (103), Expect(5) = 1e-039
Identities = 17/18 (94%), Positives = 17/18 (94%)
Frame = +1 / -1
Query: 412 NDCPAGGVGIYNPGFWGM 465
N CPAGGVGIYNPGFWGM
Sbjct: 72183 NICPAGGVGIYNPGFWGM 72130
Score = 49.1 bits (101), Expect(5) = 1e-039
Identities = 22/37 (59%), Positives = 29/37 (78%)
Frame = +1 / -3
Query: 466 NIEDGKTYHLVMYVKSPKTTCLTVSLTSSDGLQNLAS 576
NIE GK Y +V+YV+S + ++VSLTSSDGLQ LA+
Sbjct: 72022 NIEQGKIYKVVLYVRSLGSINVSVSLTSSDGLQILAT 71912
Score = 47.3 bits (97), Expect(5) = 1e-039
Identities = 17/22 (77%), Positives = 19/22 (86%)
Frame = +1 / -3
Query: 205 FFEEMGHGGAGGIWAELVSNRG 270
F +E+ H GAGGIWAELVSNRG
Sbjct: 72742 FLQEINHAGAGGIWAELVSNRG 72677
Score = 27.1 bits (53), Expect(5) = 1e-039
Identities = 10/24 (41%), Positives = 14/24 (58%)
Frame = +1 / -2
Query: 142 TLKVDASPKLARKIPDTFLGVFFE 213
T K+ + R IP+T G+FFE
Sbjct: 72935 TAKLIVNASSGRPIPETLFGIFFE 72864
>gb|DN501581.1|DN501581 V006H08.5pR Populus male catkins cDNA library Populus trichocarpa
cDNA clone V006H08 5', mRNA sequence
Length = 508
Score = 147 bits (316), Expect = 6e-035
Identities = 56/79 (70%), Positives = 65/79 (82%)
Frame = +1 / +2
Query: 175 RKIPDTFLGVFFEEMGHGGAGGIWAELVSNRGFEAGGPNTPSNIDPWLIVGDDSSVYVET 354
R IP+T G+FFEE+ H GAGGIWAELV+NRGFE+GG NTPSNI PW ++GD SS+ V T
Sbjct: 272 RPIPETLFGIFFEEINHAGAGGIWAELVNNRGFESGGQNTPSNIAPWSLIGDQSSLIVST 451
Query: 355 DRSSCFSRNIVALRMEVLC 411
DRSSCF RN ALRM+VLC
Sbjct: 452 DRSSCFERNNAALRMDVLC 508
>gb|AJ774822.1|AJ774822 AJ774822 Populus euphratica shoot 3-6 months Populus euphratica
cDNA clone P0000300024F09F1, mRNA sequence
Length = 538
Score = 131 bits (281), Expect = 4e-030
Identities = 50/68 (73%), Positives = 56/68 (82%)
Frame = +1 / +2
Query: 175 RKIPDTFLGVFFEEMGHGGAGGIWAELVSNRGFEAGGPNTPSNIDPWLIVGDDSSVYVET 354
R IP+T G+FFEE+ H GAGGIWAELV+NRGFEAGG NTPSNI PW I+GD SS+ V T
Sbjct: 335 RPIPETLFGIFFEEINHAGAGGIWAELVNNRGFEAGGQNTPSNIAPWSIIGDQSSLIVST 514
Query: 355 DRSSCFSR 378
DRSSCF R
Sbjct: 515 DRSSCFER 538
Database: Populus_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:49 PM
Number of letters in database: 203,408,664
Number of sequences in database: 369,679
Lambda K H
0.317 0.136 0.423
Matrix: BLOSUM62
Number of Hits to DB: 342,469,103
Number of Sequences: 369679
Number of extensions: 5233202
Number of successful extensions: 65510
Number of sequences better than 1.0e-020: 4
length of database: 67,802,888
effective HSP length: 49
effective length of database: 49,688,617
effective search space used: 7602358401
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.6 bits)