TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QBN10e11.xg.2.2
(581 letters)
Database: Populus_nucl_with_EST.fasta
369,679 sequences; 203,408,664 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CX178726.1|CX178726 F03_45-1_11.ab1 leaf inoculated with... 129 1e-037
gb|CX179995.1|CX179995 E03_45-30_09.ab1 leaf inoculated wit... 129 1e-037
gb|CX185263.1|CX185263 C10_45-118_06.ab1 leaf inoculated wi... 129 1e-037
gb|CX186437.1|CX186437 E12_45-118_10.ab1 leaf inoculated wi... 129 1e-037
gb|CA927208.1|CA927208 MTU6CR.P6.C08 Aspen root cDNA Librar... 129 1e-037
gb|CX173575.1|CX173575 F02_69-47_12.ab1 leaf inoculated wit... 129 1e-037
gb|DT506827.1|DT506827 WS02416.BR_J23 PTxD-ICC-N-A-14 Popul... 129 1e-037
gb|CX184516.1|CX184516 A02_45-70_02.ab1 leaf inoculated wit... 127 4e-037
gb|CF936952.1|CF936952 PO3012D09 Populus tomentiglandulosa ... 129 2e-036
gb|CN518640.1|CN518640 GQ0103.B3_B21 GQ010 Populus trichoca... 129 2e-029
gb|CV268173.1|CV268173 WS0204.B21_M02 PTxN-IB-N-A-11 Populu... 129 2e-029
>gb|CX178726.1|CX178726 F03_45-1_11.ab1 leaf inoculated with Marssonia pathogen of Populus
euramericana Populus x canadensis cDNA, mRNA sequence
Length = 619
Score = 129 bits (276), Expect(2) = 1e-037
Identities = 51/66 (77%), Positives = 59/66 (89%)
Frame = +3 / +1
Query: 216 RYPGETSREAIKRHQHFMALQLGLKKGMKVLDVGCGIGGPLIEIARFSSTSITGLTNNDY 395
R+ GE+ RE+IKRH+HF+ALQLGLK G KVLDVGCGIGGPL EIARFSSTS+TGL NN+Y
Sbjct: 388 RFKGESLRESIKRHEHFLALQLGLKPGQKVLDVGCGIGGPLREIARFSSTSVTGLNNNEY 567
Query: 396 HVSRGK 413
+SRGK
Sbjct: 568 QISRGK 585
Score = 98.2 bits (208), Expect(2) = 1e-023
Identities = 41/67 (61%), Positives = 47/67 (70%)
Frame = -2 / -3
Query: 424 KMQTLPLETW*SLLVNPVIEVELNLAISIKGPPIPHPTSRTFIPFLSPNCSAIKCWCRLM 245
+ + PL+ SLL NPV +VELN AIS+KGPPIPHPTS TF P P+C A KC CRLM
Sbjct: 596 RFSSFPLDI*YSLLFNPVTDVELNRAISLKGPPIPHPTSNTFCPGFRPSCKARKCSCRLM 417
Query: 244 ASREVSP 224
SR SP
Sbjct: 416 LSRRDSP 396
Score = 48.7 bits (100), Expect(2) = 1e-037
Identities = 16/19 (84%), Positives = 18/19 (94%)
Frame = +3 / +1
Query: 3 YDLVTGFYEYGWGDSFHFA 59
YDLVT FYE+GWG+SFHFA
Sbjct: 328 YDLVTSFYEFGWGESFHFA 384
Score = 32.2 bits (64), Expect(2) = 1e-023
Identities = 14/19 (73%), Positives = 15/19 (78%)
Frame = -2 / -3
Query: 58 AKWNESPHPYS*KPVTRS* 2
AKW +SP P S*K VTRS*
Sbjct: 383 AKWKDSPQPNS*KLVTRS* 327
>gb|CX179995.1|CX179995 E03_45-30_09.ab1 leaf inoculated with Marssonia pathogen of Populus
euramericana Populus x canadensis cDNA, mRNA sequence
Length = 622
Score = 129 bits (276), Expect(2) = 1e-037
Identities = 51/66 (77%), Positives = 59/66 (89%)
Frame = +3 / +1
Query: 216 RYPGETSREAIKRHQHFMALQLGLKKGMKVLDVGCGIGGPLIEIARFSSTSITGLTNNDY 395
R+ GE+ RE+IKRH+HF+ALQLGLK G KVLDVGCGIGGPL EIARFSSTS+TGL NN+Y
Sbjct: 379 RFKGESLRESIKRHEHFLALQLGLKPGQKVLDVGCGIGGPLREIARFSSTSVTGLNNNEY 558
Query: 396 HVSRGK 413
+SRGK
Sbjct: 559 QISRGK 576
Score = 98.2 bits (208), Expect(2) = 1e-023
Identities = 41/67 (61%), Positives = 47/67 (70%)
Frame = -2 / -3
Query: 424 KMQTLPLETW*SLLVNPVIEVELNLAISIKGPPIPHPTSRTFIPFLSPNCSAIKCWCRLM 245
+ + PL+ SLL NPV +VELN AIS+KGPPIPHPTS TF P P+C A KC CRLM
Sbjct: 587 RFSSFPLDI*YSLLFNPVTDVELNRAISLKGPPIPHPTSNTFCPGFRPSCKARKCSCRLM 408
Query: 244 ASREVSP 224
SR SP
Sbjct: 407 LSRRDSP 387
Score = 48.7 bits (100), Expect(2) = 1e-037
Identities = 16/19 (84%), Positives = 18/19 (94%)
Frame = +3 / +1
Query: 3 YDLVTGFYEYGWGDSFHFA 59
YDLVT FYE+GWG+SFHFA
Sbjct: 319 YDLVTSFYEFGWGESFHFA 375
Score = 32.2 bits (64), Expect(2) = 1e-023
Identities = 14/19 (73%), Positives = 15/19 (78%)
Frame = -2 / -3
Query: 58 AKWNESPHPYS*KPVTRS* 2
AKW +SP P S*K VTRS*
Sbjct: 374 AKWKDSPQPNS*KLVTRS* 318
>gb|CX185263.1|CX185263 C10_45-118_06.ab1 leaf inoculated with Marssonia pathogen of
Populus euramericana Populus x canadensis cDNA, mRNA
sequence
Length = 660
Score = 129 bits (276), Expect(2) = 1e-037
Identities = 51/66 (77%), Positives = 59/66 (89%)
Frame = +3 / +2
Query: 216 RYPGETSREAIKRHQHFMALQLGLKKGMKVLDVGCGIGGPLIEIARFSSTSITGLTNNDY 395
R+ GE+ RE+IKRH+HF+ALQLGLK G KVLDVGCGIGGPL EIARFSSTS+TGL NN+Y
Sbjct: 395 RFKGESLRESIKRHEHFLALQLGLKPGQKVLDVGCGIGGPLREIARFSSTSVTGLNNNEY 574
Query: 396 HVSRGK 413
+SRGK
Sbjct: 575 QISRGK 592
Score = 99.1 bits (210), Expect(2) = 8e-024
Identities = 43/80 (53%), Positives = 50/80 (62%)
Frame = -2 / -1
Query: 463 PYAIQFXCSKKKAKMQTLPLETW*SLLVNPVIEVELNLAISIKGPPIPHPTSRTFIPFLS 284
P+ S + + PL+ SLL NPV +VELN AIS+KGPPIPHPTS TF P
Sbjct: 642 PFTKSQVLSTPAMRFSSFPLDI*YSLLFNPVTDVELNRAISLKGPPIPHPTSNTFCPGFR 463
Query: 283 PNCSAIKCWCRLMASREVSP 224
P+C A KC CRLM SR SP
Sbjct: 462 PSCKARKCSCRLMLSRRDSP 403
Score = 48.7 bits (100), Expect(2) = 1e-037
Identities = 16/19 (84%), Positives = 18/19 (94%)
Frame = +3 / +2
Query: 3 YDLVTGFYEYGWGDSFHFA 59
YDLVT FYE+GWG+SFHFA
Sbjct: 335 YDLVTSFYEFGWGESFHFA 391
Score = 32.2 bits (64), Expect(2) = 8e-024
Identities = 14/19 (73%), Positives = 15/19 (78%)
Frame = -2 / -1
Query: 58 AKWNESPHPYS*KPVTRS* 2
AKW +SP P S*K VTRS*
Sbjct: 390 AKWKDSPQPNS*KLVTRS* 334
>gb|CX186437.1|CX186437 E12_45-118_10.ab1 leaf inoculated with Marssonia pathogen of
Populus euramericana Populus x canadensis cDNA, mRNA
sequence
Length = 669
Score = 129 bits (276), Expect(2) = 1e-037
Identities = 51/66 (77%), Positives = 59/66 (89%)
Frame = +3 / +2
Query: 216 RYPGETSREAIKRHQHFMALQLGLKKGMKVLDVGCGIGGPLIEIARFSSTSITGLTNNDY 395
R+ GE+ RE+IKRH+HF+ALQLGLK G KVLDVGCGIGGPL EIARFSSTS+TGL NN+Y
Sbjct: 395 RFKGESLRESIKRHEHFLALQLGLKPGQKVLDVGCGIGGPLREIARFSSTSVTGLNNNEY 574
Query: 396 HVSRGK 413
+SRGK
Sbjct: 575 QISRGK 592
Score = 99.1 bits (210), Expect(2) = 8e-024
Identities = 43/80 (53%), Positives = 50/80 (62%)
Frame = -2 / -1
Query: 463 PYAIQFXCSKKKAKMQTLPLETW*SLLVNPVIEVELNLAISIKGPPIPHPTSRTFIPFLS 284
P+ S + + PL+ SLL NPV +VELN AIS+KGPPIPHPTS TF P
Sbjct: 642 PFTKSQVLSTXAMRFSSFPLDI*YSLLFNPVTDVELNRAISLKGPPIPHPTSNTFCPGFR 463
Query: 283 PNCSAIKCWCRLMASREVSP 224
P+C A KC CRLM SR SP
Sbjct: 462 PSCKARKCSCRLMLSRRDSP 403
Score = 48.7 bits (100), Expect(2) = 1e-037
Identities = 16/19 (84%), Positives = 18/19 (94%)
Frame = +3 / +2
Query: 3 YDLVTGFYEYGWGDSFHFA 59
YDLVT FYE+GWG+SFHFA
Sbjct: 335 YDLVTSFYEFGWGESFHFA 391
Score = 32.2 bits (64), Expect(2) = 8e-024
Identities = 14/19 (73%), Positives = 15/19 (78%)
Frame = -2 / -1
Query: 58 AKWNESPHPYS*KPVTRS* 2
AKW +SP P S*K VTRS*
Sbjct: 390 AKWKDSPQPNS*KLVTRS* 334
>gb|CA927208.1|CA927208 MTU6CR.P6.C08 Aspen root cDNA Library Populus tremuloides cDNA,
mRNA sequence
Length = 684
Score = 129 bits (276), Expect(2) = 1e-037
Identities = 51/66 (77%), Positives = 59/66 (89%)
Frame = +3 / -1
Query: 216 RYPGETSREAIKRHQHFMALQLGLKKGMKVLDVGCGIGGPLIEIARFSSTSITGLTNNDY 395
R+ GE+ RE+IKRH+HF+ALQLGLK G KVLDVGCGIGGPL EIARFSSTS+TGL NN+Y
Sbjct: 576 RFKGESLRESIKRHEHFLALQLGLKPGQKVLDVGCGIGGPLREIARFSSTSVTGLNNNEY 397
Query: 396 HVSRGK 413
+SRGK
Sbjct: 396 QISRGK 379
Score = 98.7 bits (209), Expect(2) = 1e-023
Identities = 40/67 (59%), Positives = 49/67 (73%)
Frame = -2 / +2
Query: 424 KMQTLPLETW*SLLVNPVIEVELNLAISIKGPPIPHPTSRTFIPFLSPNCSAIKCWCRLM 245
++ + PL+ SLL NPV +VELN AIS++GPPIPHPTS+TF P P+C A KC CRLM
Sbjct: 368 RLSSFPLDI*YSLLFNPVTDVELNRAISLRGPPIPHPTSKTFCPGFRPSCKARKCSCRLM 547
Query: 244 ASREVSP 224
SR SP
Sbjct: 548 LSRRDSP 568
Score = 48.7 bits (100), Expect(2) = 1e-037
Identities = 16/19 (84%), Positives = 18/19 (94%)
Frame = +3 / -1
Query: 3 YDLVTGFYEYGWGDSFHFA 59
YDLVT FYE+GWG+SFHFA
Sbjct: 636 YDLVTSFYEFGWGESFHFA 580
Score = 32.2 bits (64), Expect(2) = 1e-023
Identities = 14/19 (73%), Positives = 15/19 (78%)
Frame = -2 / +2
Query: 58 AKWNESPHPYS*KPVTRS* 2
AKW +SP P S*K VTRS*
Sbjct: 581 AKWKDSPQPNS*KLVTRS* 637
>gb|CX173575.1|CX173575 F02_69-47_12.ab1 leaf inoculated with Marssonia pathogen of Populus
deltoides Populus deltoides cDNA, mRNA sequence
Length = 695
Score = 129 bits (276), Expect(2) = 1e-037
Identities = 51/66 (77%), Positives = 59/66 (89%)
Frame = +3 / +2
Query: 216 RYPGETSREAIKRHQHFMALQLGLKKGMKVLDVGCGIGGPLIEIARFSSTSITGLTNNDY 395
R+ GE+ RE+IKRH+HF+ALQLGLK G KVLDVGCGIGGPL EIARFSSTS+TGL NN+Y
Sbjct: 386 RFKGESLRESIKRHEHFLALQLGLKPGQKVLDVGCGIGGPLREIARFSSTSVTGLNNNEY 565
Query: 396 HVSRGK 413
+SRGK
Sbjct: 566 QISRGK 583
Score = 99.1 bits (210), Expect(2) = 9e-024
Identities = 43/80 (53%), Positives = 50/80 (62%)
Frame = -2 / -3
Query: 463 PYAIQFXCSKKKAKMQTLPLETW*SLLVNPVIEVELNLAISIKGPPIPHPTSRTFIPFLS 284
P+ S + + PL+ SLL NPV +VELN AIS+KGPPIPHPTS TF P
Sbjct: 633 PFTKSQVLSTPAMRFSSFPLDI*YSLLFNPVTDVELNRAISLKGPPIPHPTSNTFCPGFR 454
Query: 283 PNCSAIKCWCRLMASREVSP 224
P+C A KC CRLM SR SP
Sbjct: 453 PSCKARKCSCRLMLSRRDSP 394
Score = 48.7 bits (100), Expect(2) = 1e-037
Identities = 16/19 (84%), Positives = 18/19 (94%)
Frame = +3 / +2
Query: 3 YDLVTGFYEYGWGDSFHFA 59
YDLVT FYE+GWG+SFHFA
Sbjct: 326 YDLVTSFYEFGWGESFHFA 382
Score = 32.2 bits (64), Expect(2) = 9e-024
Identities = 14/19 (73%), Positives = 15/19 (78%)
Frame = -2 / -3
Query: 58 AKWNESPHPYS*KPVTRS* 2
AKW +SP P S*K VTRS*
Sbjct: 381 AKWKDSPQPNS*KLVTRS* 325
>gb|DT506827.1|DT506827 WS02416.BR_J23 PTxD-ICC-N-A-14 Populus trichocarpa x Populus
deltoides cDNA clone WS02416_J23 5', mRNA sequence
Length = 861
Score = 129 bits (276), Expect(2) = 1e-037
Identities = 51/66 (77%), Positives = 59/66 (89%)
Frame = +3 / +3
Query: 216 RYPGETSREAIKRHQHFMALQLGLKKGMKVLDVGCGIGGPLIEIARFSSTSITGLTNNDY 395
R+ GE+ RE+IKRH+HF+ALQLGLK G KVLDVGCGIGGPL EIARFSSTS+TGL NN+Y
Sbjct: 450 RFKGESLRESIKRHEHFLALQLGLKPGQKVLDVGCGIGGPLREIARFSSTSVTGLNNNEY 629
Query: 396 HVSRGK 413
+SRGK
Sbjct: 630 QISRGK 647
Score = 98.7 bits (209), Expect(2) = 1e-023
Identities = 40/67 (59%), Positives = 49/67 (73%)
Frame = -2 / -3
Query: 424 KMQTLPLETW*SLLVNPVIEVELNLAISIKGPPIPHPTSRTFIPFLSPNCSAIKCWCRLM 245
++ + PL+ SLL NPV +VELN AIS++GPPIPHPTS+TF P P+C A KC CRLM
Sbjct: 658 RLSSFPLDI*YSLLFNPVTDVELNRAISLRGPPIPHPTSKTFCPGFRPSCKARKCSCRLM 479
Query: 244 ASREVSP 224
SR SP
Sbjct: 478 LSRRDSP 458
Score = 48.7 bits (100), Expect(2) = 1e-037
Identities = 16/19 (84%), Positives = 18/19 (94%)
Frame = +3 / +3
Query: 3 YDLVTGFYEYGWGDSFHFA 59
YDLVT FYE+GWG+SFHFA
Sbjct: 390 YDLVTSFYEFGWGESFHFA 446
Score = 32.2 bits (64), Expect(2) = 1e-023
Identities = 14/19 (73%), Positives = 15/19 (78%)
Frame = -2 / -3
Query: 58 AKWNESPHPYS*KPVTRS* 2
AKW +SP P S*K VTRS*
Sbjct: 445 AKWKDSPQPNS*KLVTRS* 389
>gb|CX184516.1|CX184516 A02_45-70_02.ab1 leaf inoculated with Marssonia pathogen of Populus
euramericana Populus x canadensis cDNA, mRNA sequence
Length = 746
Score = 127 bits (272), Expect(2) = 4e-037
Identities = 50/66 (75%), Positives = 58/66 (87%)
Frame = +3 / +2
Query: 216 RYPGETSREAIKRHQHFMALQLGLKKGMKVLDVGCGIGGPLIEIARFSSTSITGLTNNDY 395
R+ GE+ RE+IKRH+HF+ALQLGLK G KVLDVGCGIGGPL EIARFSSTS+TGL NN+Y
Sbjct: 407 RFKGESLRESIKRHEHFLALQLGLKPGQKVLDVGCGIGGPLREIARFSSTSVTGLNNNEY 586
Query: 396 HVSRGK 413
+ RGK
Sbjct: 587 QIXRGK 604
Score = 97.7 bits (207), Expect(2) = 2e-023
Identities = 43/80 (53%), Positives = 49/80 (61%)
Frame = -2 / -3
Query: 463 PYAIQFXCSKKKAKMQTLPLETW*SLLVNPVIEVELNLAISIKGPPIPHPTSRTFIPFLS 284
P+ S + + PL SLL NPV +VELN AIS+KGPPIPHPTS TF P
Sbjct: 654 PFTKSQVLSTPAMRFSSFPLXI*YSLLFNPVTDVELNRAISLKGPPIPHPTSNTFCPGFR 475
Query: 283 PNCSAIKCWCRLMASREVSP 224
P+C A KC CRLM SR SP
Sbjct: 474 PSCKARKCSCRLMLSRRDSP 415
Score = 48.7 bits (100), Expect(2) = 4e-037
Identities = 16/19 (84%), Positives = 18/19 (94%)
Frame = +3 / +2
Query: 3 YDLVTGFYEYGWGDSFHFA 59
YDLVT FYE+GWG+SFHFA
Sbjct: 347 YDLVTSFYEFGWGESFHFA 403
Score = 32.2 bits (64), Expect(2) = 2e-023
Identities = 14/19 (73%), Positives = 15/19 (78%)
Frame = -2 / -3
Query: 58 AKWNESPHPYS*KPVTRS* 2
AKW +SP P S*K VTRS*
Sbjct: 402 AKWKDSPQPNS*KLVTRS* 346
>gb|CF936952.1|CF936952 PO3012D09 Populus tomentiglandulosa T. Lee cDNA library Populus
tomentiglandulosa cDNA, mRNA sequence
Length = 1079
Score = 129 bits (276), Expect(2) = 2e-036
Identities = 51/66 (77%), Positives = 59/66 (89%)
Frame = +3 / +1
Query: 216 RYPGETSREAIKRHQHFMALQLGLKKGMKVLDVGCGIGGPLIEIARFSSTSITGLTNNDY 395
R+ GE+ RE+IKRH+HF+ALQLGLK G KVLDVGCGIGGPL EIARFSSTS+TGL NN+Y
Sbjct: 322 RFKGESLRESIKRHEHFLALQLGLKPGQKVLDVGCGIGGPLREIARFSSTSVTGLNNNEY 501
Query: 396 HVSRGK 413
+SRGK
Sbjct: 502 QISRGK 519
Score = 98.2 bits (208), Expect(2) = 9e-023
Identities = 41/67 (61%), Positives = 47/67 (70%)
Frame = -2 / -1
Query: 424 KMQTLPLETW*SLLVNPVIEVELNLAISIKGPPIPHPTSRTFIPFLSPNCSAIKCWCRLM 245
+ + PL+ SLL NPV +VELN AIS+KGPPIPHPTS TF P P+C A KC CRLM
Sbjct: 530 RFSSFPLDI*YSLLFNPVTDVELNRAISLKGPPIPHPTSNTFCPGFRPSCKARKCSCRLM 351
Query: 244 ASREVSP 224
SR SP
Sbjct: 350 LSRRDSP 330
Score = 45.1 bits (92), Expect(2) = 2e-036
Identities = 15/19 (78%), Positives = 17/19 (89%)
Frame = +3 / +1
Query: 3 YDLVTGFYEYGWGDSFHFA 59
YDLVT FYE+GWG+SFH A
Sbjct: 262 YDLVTSFYEFGWGESFHSA 318
Score = 30.4 bits (60), Expect(2) = 9e-023
Identities = 13/19 (68%), Positives = 15/19 (78%)
Frame = -2 / -1
Query: 58 AKWNESPHPYS*KPVTRS* 2
A+W +SP P S*K VTRS*
Sbjct: 317 AEWKDSPQPNS*KLVTRS* 261
>gb|CN518640.1|CN518640 GQ0103.B3_B21 GQ010 Populus trichocarpa x Populus deltoides cDNA
clone GQ0103_B21 5', mRNA sequence
Length = 280
Score = 129 bits (276), Expect = 2e-029
Identities = 51/66 (77%), Positives = 59/66 (89%)
Frame = +3 / +3
Query: 216 RYPGETSREAIKRHQHFMALQLGLKKGMKVLDVGCGIGGPLIEIARFSSTSITGLTNNDY 395
R+ GE+ RE+IKRH+HF+ALQLGLK G KVLDVGCGIGGPL EIARFSSTS+TGL NN+Y
Sbjct: 18 RFKGESLRESIKRHEHFLALQLGLKPGQKVLDVGCGIGGPLREIARFSSTSVTGLNNNEY 197
Query: 396 HVSRGK 413
+SRGK
Sbjct: 198 QISRGK 215
>gb|CV268173.1|CV268173 WS0204.B21_M02 PTxN-IB-N-A-11 Populus trichocarpa x Populus nigra
cDNA clone WS0204_M02 3', mRNA sequence
Length = 911
Score = 129 bits (276), Expect = 2e-029
Identities = 51/66 (77%), Positives = 59/66 (89%)
Frame = +3 / +1
Query: 216 RYPGETSREAIKRHQHFMALQLGLKKGMKVLDVGCGIGGPLIEIARFSSTSITGLTNNDY 395
R+ GE+ RE+IKRH+HF+ALQLGLK G KVLDVGCGIGGPL EIARFSSTS+TGL NN+Y
Sbjct: 616 RFKGESLRESIKRHEHFLALQLGLKPGQKVLDVGCGIGGPLREIARFSSTSVTGLNNNEY 795
Query: 396 HVSRGK 413
+SRGK
Sbjct: 796 QISRGK 813
Database: Populus_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:49 PM
Number of letters in database: 203,408,664
Number of sequences in database: 369,679
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 270,855,861
Number of Sequences: 369679
Number of extensions: 3757675
Number of successful extensions: 56118
Number of sequences better than 1.0e-020: 16
length of database: 67,802,888
effective HSP length: 51
effective length of database: 48,949,259
effective search space used: 6950794778
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)