TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QAT2e04.yg.2.1
(424 letters)
Database: Populus_nucl_with_EST.fasta
369,679 sequences; 203,408,664 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BI127543.1|BI127543 G062P08Y Populus cambium cDNA librar... 265 1e-070
gb|AJ778206.1|AJ778206 AJ778206 Populus euphratica root 3-6... 226 8e-059
gb|DN493538.1|DN493538 C044P02.5pR Populus strain T89 leave... 186 6e-047
gb|CX172758.1|CX172758 F08_69-12_12.ab1 leaf inoculated wit... 171 2e-042
gb|CN519694.1|CN519694 GQ0104.B3_A15 GQ010 Populus trichoca... 132 2e-030
gb|AI163781.1|AI163781 A048p19u Hybrid aspen plasmid librar... 75 3e-027
>gb|BI127543.1|BI127543 G062P08Y Populus cambium cDNA library Populus tremula x Populus
tremuloides cDNA, mRNA sequence
Length = 457
Score = 265 bits (573), Expect = 1e-070
Identities = 107/139 (76%), Positives = 125/139 (89%)
Frame = +3 / +2
Query: 3 ATALPKAHPSFSLQQVLEMSERWKARTVPEEPIHLFIGILSATNHFAERMAIRKTWMQFP 182
AT+LP++HPSFS Q+VLEMSE+WKA +P+ PI +FIGILSATNHFAERMA+RKTWMQ
Sbjct: 32 ATSLPRSHPSFSPQRVLEMSEKWKAHPLPKRPIQVFIGILSATNHFAERMAVRKTWMQSS 211
Query: 183 AIQSGNAVARFFVALSHRKEINAALKKEAEYFGDIVILPFIDRYELVVLKTVAICQYGVH 362
AI+S N VARFFVAL+ RKE+NA LKKEA YFGDIVILPF+DRYELVVLKT+AIC++GV
Sbjct: 212 AIKSSNVVARFFVALNPRKEVNAVLKKEAAYFGDIVILPFMDRYELVVLKTIAICEFGVQ 391
Query: 363 NVTADYIMKCDDDTFVRLD 419
NV+A YIMKCDDDTFVR+D
Sbjct: 392 NVSAAYIMKCDDDTFVRVD 448
Score = 158 bits (340), Expect = 2e-038
Identities = 78/141 (55%), Positives = 90/141 (63%)
Frame = -1 / -2
Query: 424 TISSRTKVSSSHFMM*SAVTLCTPY*HIATVFRTTSSYRSMNGRMTMSPKYXXXXXXXXX 245
T+S+ T VSSSHF+M +A T TP IA V RTTSSY S+NG++T+SPKY
Sbjct: 453 TVSTLTNVSSSHFIMYAAETF*TPNSQIAIVLRTTSSYLSINGKITISPKYAASFFSTAF 274
Query: 244 XXXXXXXATKNRATAFPDWIAGNCIQVFLIAMRSAKWFVAERMPMKRWIGSSGTVRAFHL 65
ATK RAT F D IA +CIQVFL AMRSAK FVA+R+P+K IG G AFH
Sbjct: 273 TSFLGFNATKKRATTFDDLIADDCIQVFLTAMRSAK*FVADRIPIKT*IGLFGKG*AFHF 94
Query: 64 SDISKTCWREKLGCAFGSAVA 2
SDIS T EKLGC G VA
Sbjct: 93 SDISNTL*GEKLGCDLGREVA 31
Score = 118 bits (252), Expect = 2e-026
Identities = 54/132 (40%), Positives = 79/132 (59%)
Frame = -3 / -1
Query: 419 IQPHKGVIVALHDVICSDIVHPVLTYRNRFQNH*LIPVDERQNDYVSKILCFLLQCCIYL 240
+ P+K +I+ H+V +I++P LT N F+NH LI + +RQN ++K+ C CI+L
Sbjct: 448 VNPNKCIIITFHNVRSRNILNPKLTNSNSFKNHKLISIHKRQNHNITKVCCLFF*HCIHL 269
Query: 239 LSVAQRNKEPSNRIPRLDSRKLHPSFPNSHALGEMVCGGEDADEKMDWFLWHSASLPPFR 60
L Q NK+ SN I LD LHPSF N HA +++C + ++ ++W LW S P F
Sbjct: 268 LPWIQCNKKTSNYI**LDC**LHPSFSNCHAFCKVICS**NPNKNLNWPLW*RMSFPLF* 89
Query: 59 HFQDLLERKARM 24
HFQ L R+A M
Sbjct: 88 HFQYSLRREAWM 53
Score = 111 bits (237), Expect = 2e-024
Identities = 49/129 (37%), Positives = 76/129 (58%)
Frame = +2 / +1
Query: 23 TS*LFSPASLGNV*KVEGSHCARGTNPSFHRHPLRHKPFRRAHGY*ENLDAVSGYPIGEC 202
TS L S S+GNV KVE S + N SF+ + +K R HG *+NLDA+ + +C
Sbjct: 52 TSKLLSSKSIGNVRKVESSSFTKEANSSFYWDSISYKSLCRTHGS*KNLDAIISNQVIKC 231
Query: 203 GCSVLCCAEPQKGDKCSTEEGSRVFWRHSHSAVHRPV*ASGSENGCDMSIRGAQCHCRLH 382
CS CC E ++G +CS ++ + W + AV+ +*A GS N C + + G++C C +H
Sbjct: 232 SCSFFCCIESKEGGECSAKKRGSILW*YCDFAVYG*I*ACGS*NYCYL*VWGSECFCCVH 411
Query: 383 HEVRR*HLC 409
+E+ *++C
Sbjct: 412 YEM***YIC 438
>gb|AJ778206.1|AJ778206 AJ778206 Populus euphratica root 3-6 months Populus euphratica cDNA
clone P0000400040H01F1, mRNA sequence
Length = 682
Score = 226 bits (487), Expect = 8e-059
Identities = 94/139 (67%), Positives = 111/139 (79%)
Frame = +3 / +3
Query: 3 ATALPKAHPSFSLQQVLEMSERWKARTVPEEPIHLFIGILSATNHFAERMAIRKTWMQFP 182
A +LP HPSFS Q+ LEMS RWKA + P+ LFIG+LSA NHF+ERMA+RK+WMQ
Sbjct: 36 AASLPSNHPSFSPQKHLEMSSRWKAPPLSVGPVELFIGVLSAGNHFSERMAVRKSWMQHR 215
Query: 183 AIQSGNAVARFFVALSHRKEINAALKKEAEYFGDIVILPFIDRYELVVLKTVAICQYGVH 362
I+S N VARFFVAL RKE+N LKKEAE+FGDIVI+P++D Y+LVVLKTVAIC+YGV
Sbjct: 216 LIKSSNVVARFFVALHARKEVNLELKKEAEFFGDIVIVPYMDNYDLVVLKTVAICEYGVR 395
Query: 363 NVTADYIMKCDDDTFVRLD 419
V A YIMK DDDTFVR+D
Sbjct: 396 TVRAKYIMKGDDDTFVRVD 452
Score = 108 bits (230), Expect = 2e-023
Identities = 64/139 (46%), Positives = 76/139 (54%)
Frame = -1 / -1
Query: 418 SSRTKVSSSHFMM*SAVTLCTPY*HIATVFRTTSSYRSMNGRMTMSPKYXXXXXXXXXXX 239
S+ T VSSS FM+ A T+ TPY +ATVF TT S SM G +T+SPK
Sbjct: 451 STLTNVSSSPFMIYFARTVRTPYSQMATVFNTTRS*LSM*GTITISPKNSASFFNSKFTS 272
Query: 238 XXXXXATKNRATAFPDWIAGNCIQVFLIAMRSAKWFVAERMPMKRWIGSSGTVRAFHLSD 59
ATK RAT F D I+ CIQ FL A+RS K AER P+K G + AFHL D
Sbjct: 271 FRACSATKKRATTFEDLISLCCIQDFLTAIRSEK*LPAERTPIKSSTGPTERGGAFHLLD 92
Query: 58 ISKTCWREKLGCAFGSAVA 2
IS+ EKLG G+ A
Sbjct: 91 ISRCF*GEKLG*FEGNEAA 35
>gb|DN493538.1|DN493538 C044P02.5pR Populus strain T89 leaves Populus tremula x Populus
tremuloides cDNA clone C044P02 5', mRNA sequence
Length = 598
Score = 186 bits (401), Expect = 6e-047
Identities = 77/101 (76%), Positives = 86/101 (85%)
Frame = +3 / +1
Query: 117 ILSATNHFAERMAIRKTWMQFPAIQSGNAVARFFVALSHRKEINAALKKEAEYFGDIVIL 296
+LSA NHFAERMA+RKTWMQ I+S VARFFVAL+ RKEIN LKKEAEYFGDIVI+
Sbjct: 1 VLSAGNHFAERMAVRKTWMQHELIRSSKIVARFFVALNGRKEINVDLKKEAEYFGDIVIV 180
Query: 297 PFIDRYELVVLKTVAICQYGVHNVTADYIMKCDDDTFVRLD 419
P++D YELVVLKTVAIC+YGV V A YIMKCDDDTFVR+D
Sbjct: 181 PYMDNYELVVLKTVAICEYGVRTVDAKYIMKCDDDTFVRVD 303
Score = 114 bits (243), Expect = 4e-025
Identities = 57/100 (57%), Positives = 64/100 (64%)
Frame = -1 / -3
Query: 418 SSRTKVSSSHFMM*SAVTLCTPY*HIATVFRTTSSYRSMNGRMTMSPKYXXXXXXXXXXX 239
S+ TKVSSSHFM+ A T+ TPY IATVF+TTSS SM G +T+SPKY
Sbjct: 302 STLTKVSSSHFMIYFASTVRTPYSQIATVFKTTSS*LSM*GTITISPKYSASFFRSTFIS 123
Query: 238 XXXXXATKNRATAFPDWIAGNCIQVFLIAMRSAKWFVAER 119
ATKNRAT F D I CIQVFL A+RSAKW AER
Sbjct: 122 FLPFNATKNRATIFEDLINSCCIQVFLTAIRSAKWLPAER 3
>gb|CX172758.1|CX172758 F08_69-12_12.ab1 leaf inoculated with Marssonia pathogen of Populus
deltoides Populus deltoides cDNA, mRNA sequence
Length = 593
Score = 171 bits (368), Expect = 2e-042
Identities = 71/108 (65%), Positives = 86/108 (79%)
Frame = +3 / +3
Query: 3 ATALPKAHPSFSLQQVLEMSERWKARTVPEEPIHLFIGILSATNHFAERMAIRKTWMQFP 182
A +LP +HPSF+ QQ LEM ++W+A +P + LFIG+LSA NHFAERMA+RKTWMQ
Sbjct: 270 AASLPASHPSFAPQQHLEMFKKWQAPPLPNDQAELFIGVLSAGNHFAERMAVRKTWMQHE 449
Query: 183 AIQSGNAVARFFVALSHRKEINAALKKEAEYFGDIVILPFIDRYELVV 326
I+S VARFFVAL+ RKEIN LKKEAEYFGDIVI+P++D YELVV
Sbjct: 450 LIRSSKIVARFFVALNGRKEINVDLKKEAEYFGDIVIVPYMDNYELVV 593
Score = 102 bits (217), Expect = 1e-021
Identities = 53/108 (49%), Positives = 58/108 (53%)
Frame = -1 / -2
Query: 325 TTSSYRSMNGRMTMSPKYXXXXXXXXXXXXXXXXATKNRATAFPDWIAGNCIQVFLIAMR 146
TTSS SM G +T+SPKY ATKNRAT F D I CIQVFL A+R
Sbjct: 592 TTSS*LSM*GTITISPKYSASFFRSTFISFLPFNATKNRATIFEDLINSCCIQVFLTAIR 413
Query: 145 SAKWFVAERMPMKRWIGSSGTVRAFHLSDISKTCWREKLGCAFGSAVA 2
SAKW AER PMK S G A H +IS+ C KLGC G A
Sbjct: 412 SAKWLPAERTPMKSSAWSLGRGGACHFLNISRCCCGAKLGCEAGREAA 269
>gb|CN519694.1|CN519694 GQ0104.B3_A15 GQ010 Populus trichocarpa x Populus deltoides cDNA
clone GQ0104_A15 5', mRNA sequence
Length = 774
Score = 132 bits (282), Expect = 2e-030
Identities = 50/104 (48%), Positives = 69/104 (66%)
Frame = +3 / +2
Query: 108 FIGILSATNHFAERMAIRKTWMQFPAIQSGNAVARFFVALSHRKEINAALKKEAEYFGDI 287
FIG+ S N+ RMA+R+TWMQ+ A++SG RFFV L + +N L EA +GDI
Sbjct: 2 FIGVFSTANNXXRRMAVRRTWMQYAAVRSGAVAVRFFVGLHKNQIVNEELWNEARTYGDI 181
Query: 288 VILPFIDRYELVVLKTVAICQYGVHNVTADYIMKCDDDTFVRLD 419
++PF+D Y L+ KT+AIC +G +A Y+MK DDD FVR+D
Sbjct: 182 QLMPFVDYYNLITFKTLAICIFGTEVASAKYVMKTDDDAFVRVD 313
>gb|AI163781.1|AI163781 A048p19u Hybrid aspen plasmid library Populus tremula x Populus
tremuloides cDNA 5', mRNA sequence
Length = 296
Score = 74.8 bits (157), Expect(2) = 3e-027
Identities = 34/53 (64%), Positives = 39/53 (73%)
Frame = +3 / +3
Query: 183 AIQSGNAVARFFVALSHRKEINAALKKEAEYFGDIVILPFIDRYELVVLKTVA 341
A +S N VARFFVAL+ RKE+NA LKKEA YF +DRYELVVLKT+A
Sbjct: 138 ASKSSNVVARFFVALNPRKEVNAVLKKEAAYFX*YCDFAVMDRYELVVLKTIA 296
Score = 64.3 bits (134), Expect(2) = 3e-027
Identities = 26/42 (61%), Positives = 32/42 (76%)
Frame = +3 / +2
Query: 84 VPEEPIHLFIGILSATNHFAERMAIRKTWMQFPAIQSGNAVA 209
+P+ PI +FIGILSATN FAERMA+RKTWMQ A + +A
Sbjct: 5 LPKRPIQVFIGILSATNXFAERMAVRKTWMQSSATTFDDLIA 130
Database: Populus_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:49 PM
Number of letters in database: 203,408,664
Number of sequences in database: 369,679
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 234,169,185
Number of Sequences: 369679
Number of extensions: 3317556
Number of successful extensions: 116638
Number of sequences better than 1.0e-020: 8
length of database: 67,802,888
effective HSP length: 50
effective length of database: 49,318,938
effective search space used: 4438704420
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)