TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QAF25f04.yg.3.3
(1599 letters)
Database: Populus_nucl_with_EST.fasta
369,679 sequences; 203,408,664 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CX176710.1|CX176710 E05_69-3_09.ab1 leaf inoculated with... 282 4e-075
gb|CN518099.1|CN518099 GQ0091.B3_M10 GQ009 Populus trichoca... 175 2e-072
gb|BU827836.1|BU827836 K009P85P Populus apical shoot cDNA l... 193 3e-065
gb|DT513448.1|DT513448 WS02422.B21_B07 PTxD-ICC-N-A-14 Popu... 196 2e-051
gb|CX653585.1|CX653585 PO02004B07 Poplar SC cDNA library Po... 156 4e-049
gb|BU811752.1|BU811752 UL88TB08 Populus leaf cDNA library P... 176 4e-043
gb|DT508840.1|DT508840 WS02422.BR_B07 PTxD-ICC-N-A-14 Popul... 174 2e-042
gb|BU808966.1|BU808966 UL52PB01 Populus leaf cDNA library P... 163 3e-039
gb|CA924195.1|CA924195 MTU7CL.P2.H03 Aspen leaf cDNA Librar... 133 2e-030
gb|CA924314.1|CA924314 MTU7CL.P4.C03 Aspen leaf cDNA Librar... 133 2e-030
gb|BU815823.1|BU815823 N047A09 Populus bark cDNA library Po... 75 3e-025
gb|DN500454.1|DN500454 UL52PB01.5pR Populus cold stressed l... 106 3e-022
>gb|CX176710.1|CX176710 E05_69-3_09.ab1 leaf inoculated with Marssonia pathogen of Populus
deltoides Populus deltoides cDNA, mRNA sequence
Length = 430
Score = 282 bits (610), Expect = 4e-075
Identities = 117/134 (87%), Positives = 125/134 (93%)
Frame = +1 / +2
Query: 175 TIAEGGPFASRTSFVKLLRSVKRETLKLIETFVDKAEDLPHVGKQFVPPMMDPVLGDYAR 354
+IAEGGP+AS+TS+VKLLRSVKRETLKLIETF+DKAE +GKQFVPPMMDPVLGDYAR
Sbjct: 29 SIAEGGPYASKTSYVKLLRSVKRETLKLIETFLDKAEHTTQIGKQFVPPMMDPVLGDYAR 208
Query: 355 NVPDARESEVLSLFATIINKYKGEMLEDVPRIFEAVFQCTLEMITKNFEDYPEHRLKFFS 534
N+PDARESEVLSLFATIINKYK M+EDVPRIFEAVFQCTLEMITKNFEDYPEHRLKFFS
Sbjct: 209 NLPDARESEVLSLFATIINKYKAAMIEDVPRIFEAVFQCTLEMITKNFEDYPEHRLKFFS 388
Query: 535 LLRAIGTHCFQALI 576
LLRAI HCF ALI
Sbjct: 389 LLRAIAAHCFPALI 430
Score = 215 bits (465), Expect = 4e-055
Identities = 100/134 (74%), Positives = 108/134 (80%)
Frame = -2 / -2
Query: 575 INAWKQWVPIARSKEKNLRRCSG*SSKFLVIISRVHWKTASNIRGTSSSISPLYLLMIVA 396
I A KQ +ARS EKNLRRCSG*SSKF VIIS VHWKTASNIRGTSS I+ LYL +IVA
Sbjct: 429 IKAGKQCAAMARSNEKNLRRCSG*SSKFFVIISNVHWKTASNIRGTSSIIAALYLFIIVA 250
Query: 395 NKDRTSDSLASGTFLA*SPRTGSIIGGTNCFPTCGKSSALSTKVSINFRVSLLTDLKSFT 216
D+TSDSLASG FLA*SPRTGSIIGGTNCFP SALS VS++ VSLLTD KSFT
Sbjct: 249 KSDKTSDSLASGKFLA*SPRTGSIIGGTNCFPIWVVCSALSKNVSMSLSVSLLTDRKSFT 70
Query: 215 NDVLEAKGPPSAIV 174
DVL+A GPPSAI+
Sbjct: 69 *DVLDA*GPPSAIL 28
Score = 123 bits (263), Expect = 3e-027
Identities = 56/116 (48%), Positives = 77/116 (66%)
Frame = -1 / -1
Query: 564 EAMGANCTKQRKKFEAVLRIIFKVLSNHLKGALENSLKYTRHILKHFPFVLVNDCCKQGQ 385
E M ++ TK K+ +A+ II ++ HL+ LENS KY+R+I H FVLV +CCK+ Q
Sbjct: 418 ETMCSDGTK**KELKAMFWIILEIFCYHLQCTLENSFKYSRYIFYHCSFVLVYNCCKK*Q 239
Query: 384 NFRLPCIGYISSIITKNRIHHWWYKLLSYMWQIFRLVHKSLDQL*GFPLDRSQEFY 217
NF P I I SIITKNRIHHW ++LL + +F LV K L++L PL+R Q+F+
Sbjct: 238 NF*FPSIWQIPSIITKNRIHHWGHELLPNLGSMFGLVQKCLNELKCLPLNRPQKFH 71
Score = 118 bits (252), Expect = 1e-025
Identities = 57/132 (43%), Positives = 75/132 (56%)
Frame = +3 / +1
Query: 180 C*RRTLCLKDVICKTLEICQEGNPKVDRDFCGQGGRFATCRKAVCTTNDGSCSW*LC*KC 359
C RR LC+ +++C+T +C EG+ D GQG + +AV NDGSCSW*LC*+
Sbjct: 34 CRRRALCI*NILCETFAVC*EGDT*AH*DIFGQGRTYYPNWEAVRAPNDGSCSW*LC*EF 213
Query: 360 TRCKGV*SSVLVCNNH*QVQRGNA*GCASYI*GCFPVHP*DDY*EL*RLS*APPQIFFFA 539
RC G+ S V CNN+ QVQ N C S I* CFPV+ DD + LS P + F
Sbjct: 214 ARC*GIRSFVTFCNNYKQVQSCNDRRCTSNI*SCFPVYIGDDNKKFRGLSRTSP*VLFIT 393
Query: 540 SCNWHPLLPSIN 575
SC+ L P ++
Sbjct: 394 SCHRCTLFPCLD 429
>gb|CN518099.1|CN518099 GQ0091.B3_M10 GQ009 Populus trichocarpa x Populus deltoides cDNA
clone GQ0091_M10 5', mRNA sequence
Length = 532
Score = 175 bits (377), Expect(2) = 2e-072
Identities = 77/109 (70%), Positives = 89/109 (81%)
Frame = +1 / +3
Query: 847 LTEPLWDASSVPYQYTDNAMFVRDYTIKLLGTSFPNMTVAEVTKFVDGLLSSKLDLPSFK 1026
L+EPLWD ++VPY Y +NAMFVR+YTIKLLGTSFPNMT +EVT+FV+GL SK +L FK
Sbjct: 186 LSEPLWDTTTVPYPYLNNAMFVREYTIKLLGTSFPNMTASEVTQFVNGLFESKNNLSIFK 365
Query: 1027 NHIRDFLVQSKEFSAQDNKDLYXXXXXXXXXXXXXXMLAIPGLIAPSEL 1173
NHIRDFLVQSKEFSAQDNKDLY ML+IPG IAP+E+
Sbjct: 366 NHIRDFLVQSKEFSAQDNKDLYAXXXAVQXERERQRMLSIPGXIAPNEI 512
Score = 118 bits (253), Expect(2) = 2e-072
Identities = 48/58 (82%), Positives = 53/58 (91%)
Frame = +1 / +3
Query: 670 LLLEILKKFQASGFQNQFYKTYFLTIEQEIFAVLTDTFHKPGFKLHVSVLQHLFCAVD 843
LLLE+LK FQAS F NQFY++YFLTIEQEIFAVLTDTFHKPGFKLHV VLQHLFC+ +
Sbjct: 3 LLLEMLKNFQASEFCNQFYRSYFLTIEQEIFAVLTDTFHKPGFKLHVLVLQHLFCSAE 176
Score = 94.5 bits (200), Expect(3) = 6e-045
Identities = 45/72 (62%), Positives = 50/72 (69%)
Frame = -2 / -1
Query: 1067 ENSFDCTRKSLIWFLKLGRSSFELRRPSTNLVTSATVMLGNDVPKSFMV*SRTNMALSVY 888
ENSFDCTRKS + FL + R F+ P TN VTS VMLGNDVP+S MV SRTN+AL Y
Sbjct: 406 ENSFDCTRKSRM*FLNMDRLFFDSNSPLTN*VTSDAVMLGNDVPRSLMVYSRTNIALFKY 227
Query: 887 *YGTEDASHRGS 852
GT SHRGS
Sbjct: 226 G*GTVVVSHRGS 191
Score = 84.9 bits (179), Expect(3) = 6e-045
Identities = 40/58 (68%), Positives = 43/58 (74%)
Frame = -2 / -1
Query: 842 STAQNKCCSTET*SLKPGLWNVSVRTAKISCSIVKK*VL*NWFWNPEAWNFFKISNNR 669
S QNKCCST+T +LKPGL NVSV TA ISCSIVKK L*N N +A FF ISNNR
Sbjct: 175 SAEQNKCCSTKTCNLKPGL*NVSVNTANISCSIVKKYDL*N*LQNSDA*KFFSISNNR 2
Score = 73.0 bits (153), Expect(2) = 5e-027
Identities = 45/110 (40%), Positives = 60/110 (54%)
Frame = +2 / +1
Query: 836 RLMV*RSLYGTRLLYHINIRIMPCLFEITP*NS*EHHFLT*L*LR*PSLWMAFLVQNLIF 1015
R+++ +SL G I+I IM CLF TP N HH LT*L R +L +L Q
Sbjct: 175 RVVLCQSLCGILQQSLIHI*IMLCLFVSTPLNFWAHHSLT*LHQRSLNLLTDYLSQKTTC 354
Query: 1016 RASKTILEISWCSRRSSQLRITRIYTRKRLLPKEKGSANECLPFRG*LPL 1165
S+ E C++R+ LRI +I + LL + KGS EC PF+G LPL
Sbjct: 355 PYSRITFETFSCNQRNFLLRIIKISMQXXLLFRXKGSDKECFPFQGXLPL 504
Score = 72.5 bits (152), Expect(3) = 7e-031
Identities = 32/58 (55%), Positives = 42/58 (72%)
Frame = -1 / -3
Query: 846 TINRTKQVL*HRNMKLETRFMECVCEDCKNLLLNCQKVGFVELVLES*SLEFFQDLQQ 673
T+ R K VL*H+NM+L+TRF++C+C+ K LLNCQKV VEL+ E L+ Q LQQ
Sbjct: 179 TLRRAK*VL*HQNMQLKTRFVKCICQYSKYFLLNCQKV*PVELITELRRLKVLQHLQQ 6
Score = 69.3 bits (145), Expect(2) = 5e-027
Identities = 29/57 (50%), Positives = 39/57 (68%)
Frame = +3 / +2
Query: 669 PVVGDLEKIPGFRIPKPILQNLLFDN*ARDFCSPHRHIP*TWFQASCFGATTLVLCG 839
P+VGD E++ G + +LQ +LFDN*A + C R+I TWF +CFGATTL+L G
Sbjct: 2 PIVGDAEELSGV*VL*SVLQVILFDN*AGNICCIDRYISQTWF*VACFGATTLILLG 172
Score = 62.5 bits (130), Expect(3) = 7e-031
Identities = 26/56 (46%), Positives = 42/56 (75%)
Frame = -1 / -3
Query: 1050 HQEISNMVFEARKIKF*TKKAIHKLGHLSYSHVRK*CS*EFYGVISNKHGIIRILI 883
H+++SN++ E ++ F* K +++KL L SHVR+*C+ +F GV++NKH II+I I
Sbjct: 389 HEKVSNVILEYGQVVF*LK*SVNKLSDL*CSHVRE*CAQKFNGVLTNKHSIIQIWI 222
Score = 62.0 bits (129), Expect(3) = 9e-024
Identities = 30/58 (51%), Positives = 35/58 (60%)
Frame = +2 / +1
Query: 674 CWRS*KNSRLQDSKTNSTKPTF*QLSKRFLQSSQTHSINLVSSFMFRCYNTCFVRLMV 847
CWR *+ R S +ST TF*QLS+++L Q H NLV S MF CYNT F R V
Sbjct: 7 CWRC*RTFRRLSSVISSTGHTF*QLSRKYLLY*QIHFTNLVLSCMFWCYNTYFARRRV 180
Score = 53.8 bits (111), Expect(3) = 9e-024
Identities = 27/70 (38%), Positives = 40/70 (57%)
Frame = +3 / +2
Query: 855 ASMGRVFCTISIYG*CHVCSRLHHKTLRNIIS*HDCS*GDQVCGWPS*FKT*SSELQKPY 1034
AS+G +SI+ *C+VCS +HH T +II *HDC G +C * K +Q+ +
Sbjct: 194 ASVGYYNSPLSIFE*CYVCS*VHH*TSGHIIP*HDCIRGHSIC*RTI*VKKQPVHIQESH 373
Query: 1035 *RFPGAVEGV 1064
R A++G+
Sbjct: 374 SRLSRAIKGI 403
Score = 41.4 bits (84), Expect(3) = 6e-045
Identities = 16/22 (72%), Positives = 17/22 (77%)
Frame = -1 / -3
Query: 1170 FARGNQPRNGKHSLALPFSLGS 1105
F RGN P NGKHSL+LPF L S
Sbjct: 509 FIRGNXPWNGKHSLSLPFXLNS 444
Score = 38.2 bits (77), Expect(3) = 7e-031
Identities = 20/43 (46%), Positives = 23/43 (53%)
Frame = -3 / -2
Query: 1165 *GQSAPEWQAFVGAPFLFGQQPLPRIDPCYPELRTPSTAPGNL 1037
*GQ A EW+AF APF QQ D YPE + P A +L
Sbjct: 504 *GQXALEWKAFFVAPFXSEQQXXLHRDLYYPEQKIPLIARESL 376
Score = 33.1 bits (66), Expect(3) = 9e-024
Identities = 13/21 (61%), Positives = 14/21 (66%)
Frame = +3 / +2
Query: 1104 CCPKRKGAPTNACHSGADCP* 1166
CC + KGA NA HS A CP*
Sbjct: 443 CCSEXKGATKNAFHSRAYCP* 505
>gb|BU827836.1|BU827836 K009P85P Populus apical shoot cDNA library Populus tremula x Populus
tremuloides cDNA 5 prime, mRNA sequence
Length = 519
Score = 193 bits (416), Expect(2) = 3e-065
Identities = 81/117 (69%), Positives = 94/117 (80%)
Frame = +1 / +1
Query: 847 LTEPLWDASSVPYQYTDNAMFVRDYTIKLLGTSFPNMTVAEVTKFVDGLLSSKLDLPSFK 1026
L+EPLWD ++VPY Y +NAMFVR+YTIKLLGTSFPNMT +EVT+FV+GL SK +L FK
Sbjct: 112 LSEPLWDTTTVPYPYLNNAMFVREYTIKLLGTSFPNMTASEVTQFVNGLFESKNNLSIFK 291
Query: 1027 NHIRDFLVQSKEFSAQDNKDLYXXXXXXXXXXXXXXMLAIPGLIAPSELQDEMVDS* 1197
NHIRDFLVQSKEFSAQDNKDLY ML+IPGLIAP+E+QDEM+DS*
Sbjct: 292 NHIRDFLVQSKEFSAQDNKDLYAEEAAVQRERERQRMLSIPGLIAPNEIQDEMLDS* 462
Score = 95.9 bits (203), Expect(3) = 3e-042
Identities = 46/72 (63%), Positives = 50/72 (69%)
Frame = -2 / -2
Query: 1067 ENSFDCTRKSLIWFLKLGRSSFELRRPSTNLVTSATVMLGNDVPKSFMV*SRTNMALSVY 888
ENSFDCTRKS + FL + R F+ P TN VTS VMLGNDVPKS MV SRTN+AL Y
Sbjct: 332 ENSFDCTRKSRM*FLNMDRLFFDSNSPLTN*VTSDAVMLGNDVPKSLMVYSRTNIALFKY 153
Query: 887 *YGTEDASHRGS 852
GT SHRGS
Sbjct: 152 G*GTVVVSHRGS 117
Score = 77.1 bits (162), Expect(2) = 3e-065
Identities = 30/34 (88%), Positives = 32/34 (94%)
Frame = +1 / +1
Query: 742 TIEQEIFAVLTDTFHKPGFKLHVSVLQHLFCAVD 843
TIEQEIFAVLTDTFHKPGFKLHV VLQHLFC+ +
Sbjct: 1 TIEQEIFAVLTDTFHKPGFKLHVLVLQHLFCSAE 102
Score = 60.6 bits (126), Expect(3) = 2e-025
Identities = 25/56 (44%), Positives = 42/56 (75%)
Frame = -1 / -1
Query: 1050 HQEISNMVFEARKIKF*TKKAIHKLGHLSYSHVRK*CS*EFYGVISNKHGIIRILI 883
H+++SN++ + ++ F* K +++KL L SHVR+*C+ +F GV++NKH II+I I
Sbjct: 315 HEKVSNVILKYGQVVF*LK*SVNKLSDL*CSHVRE*CAQKFNGVLTNKHSIIQIWI 148
Score = 59.3 bits (123), Expect(3) = 3e-042
Identities = 25/33 (75%), Positives = 27/33 (81%)
Frame = -2 / -2
Query: 842 STAQNKCCSTET*SLKPGLWNVSVRTAKISCSI 744
S QNKCCST+T +LKPGL NVSV TA ISCSI
Sbjct: 101 SAEQNKCCSTKTCNLKPGL*NVSVNTANISCSI 3
Score = 56.5 bits (117), Expect(3) = 3e-042
Identities = 24/39 (61%), Positives = 29/39 (74%)
Frame = -1 / -1
Query: 1212 SKQGTLRIYHLVLQFARGNQPRNGKHSLALPFSLGSSLF 1096
+K +LRI HL+L F RGN+ N KHSL+LPF L SSLF
Sbjct: 477 AKNSSLRI*HLILYFIRGNKTWNRKHSLSLPFPLNSSLF 361
Score = 53.8 bits (111), Expect(3) = 2e-025
Identities = 20/35 (57%), Positives = 28/35 (80%)
Frame = -1 / -1
Query: 846 TINRTKQVL*HRNMKLETRFMECVCEDCKNLLLNC 742
T+ R K VL*H+NM+L+TRF++C+C+ K LLNC
Sbjct: 105 TLRRAK*VL*HQNMQLKTRFVKCICQYSKYFLLNC 1
Score = 40.5 bits (82), Expect(3) = 2e-025
Identities = 24/52 (46%), Positives = 27/52 (51%)
Frame = -3 / -3
Query: 1192 NLPSRLAIR*GQSAPEWQAFVGAPFLFGQQPLPRIDPCYPELRTPSTAPGNL 1037
NL S L *GQ E +AF APF QQPL D YPE + P A +L
Sbjct: 457 NLTSHLVFH*GQ*DLESKAFFVAPFPSEQQPLLHRDLYYPEQKIPLIARESL 302
>gb|DT513448.1|DT513448 WS02422.B21_B07 PTxD-ICC-N-A-14 Populus trichocarpa x Populus
deltoides cDNA clone WS02422_B07 3', mRNA sequence
Length = 777
Score = 196 bits (422), Expect(2) = 2e-051
Identities = 81/117 (69%), Positives = 95/117 (81%)
Frame = +1 / -3
Query: 847 LTEPLWDASSVPYQYTDNAMFVRDYTIKLLGTSFPNMTVAEVTKFVDGLLSSKLDLPSFK 1026
LTEPLWDA+++ Y Y +NAMFVR+YTIKLLGTSFPNMT +EVT+FV+GL S+ DL +FK
Sbjct: 727 LTEPLWDAATISYSYPNNAMFVREYTIKLLGTSFPNMTASEVTQFVNGLFESRNDLSAFK 548
Query: 1027 NHIRDFLVQSKEFSAQDNKDLYXXXXXXXXXXXXXXMLAIPGLIAPSELQDEMVDS* 1197
NHIRDFLVQSKEFSAQDNKDLY ML+IPGLIAP+E+QDEM+DS*
Sbjct: 547 NHIRDFLVQSKEFSAQDNKDLYAEEAAAQRERERQRMLSIPGLIAPNEIQDEMLDS* 377
Score = 89.5 bits (189), Expect(3) = 2e-030
Identities = 44/74 (59%), Positives = 49/74 (66%)
Frame = -2 / +3
Query: 1067 ENSFDCTRKSLIWFLKLGRSSFELRRPSTNLVTSATVMLGNDVPKSFMV*SRTNMALSVY 888
ENSFDCTRKS + FL RS + P TN VTS VM GNDVP+S MV SRTN+AL Y
Sbjct: 507 ENSFDCTRKSRM*FLNADRSFLDSNSPLTN*VTSDAVMFGNDVPRSLMVYSRTNIALFGY 686
Query: 887 *YGTEDASHRGSVR 846
ASHRGSV+
Sbjct: 687 E*EIVAASHRGSVK 728
Score = 52.8 bits (109), Expect(3) = 2e-030
Identities = 21/30 (70%), Positives = 24/30 (80%)
Frame = -1 / +2
Query: 1185 HLVLQFARGNQPRNGKHSLALPFSLGSSLF 1096
HL+L F RGN+P NGKHSL+L F L SSLF
Sbjct: 389 HLILYFIRGNKPWNGKHSLSLSFPLSSSLF 478
Score = 29.0 bits (57), Expect(3) = 2e-030
Identities = 10/12 (83%), Positives = 11/12 (91%)
Frame = -2 / +3
Query: 842 STAQNKCCSTET 807
ST QNKCCST+T
Sbjct: 738 STKQNKCCSTKT 773
Score = 28.1 bits (55), Expect(2) = 2e-051
Identities = 10/13 (76%), Positives = 11/13 (84%)
Frame = +1 / -3
Query: 805 HVSVLQHLFCAVD 843
HV VLQHLFC V+
Sbjct: 775 HVLVLQHLFCLVE 737
>gb|CX653585.1|CX653585 PO02004B07 Poplar SC cDNA library Populus alba x Populus tremula var.
glandulosa cDNA clone PO02004B07 5', mRNA sequence
Length = 369
Score = 156 bits (336), Expect(2) = 4e-049
Identities = 63/82 (76%), Positives = 72/82 (87%)
Frame = +1 / +3
Query: 847 LTEPLWDASSVPYQYTDNAMFVRDYTIKLLGTSFPNMTVAEVTKFVDGLLSSKLDLPSFK 1026
L+EPLWD ++ PY Y +NAMFVR+YTIKLLGTSFPNMT +EVT+FV+GL SK +L FK
Sbjct: 90 LSEPLWDTTTAPYPYLNNAMFVREYTIKLLGTSFPNMTASEVTQFVNGLFESKNNLSIFK 269
Query: 1027 NHIRDFLVQSKEFSAQDNKDLY 1092
NHIRDFLVQSKEFSAQDNKDLY
Sbjct: 270 NHIRDFLVQSKEFSAQDNKDLY 335
Score = 93.6 bits (198), Expect(2) = 3e-026
Identities = 45/72 (62%), Positives = 49/72 (68%)
Frame = -2 / -3
Query: 1067 ENSFDCTRKSLIWFLKLGRSSFELRRPSTNLVTSATVMLGNDVPKSFMV*SRTNMALSVY 888
ENSFDCTRKS + FL + R F+ P TN VTS VMLGNDVPKS MV SRTN+AL Y
Sbjct: 310 ENSFDCTRKSRM*FLNMDRLFFDSNSPLTN*VTSDAVMLGNDVPKSLMVYSRTNIALFKY 131
Query: 887 *YGTEDASHRGS 852
G SHRGS
Sbjct: 130 G*GAVVVSHRGS 95
Score = 59.7 bits (124), Expect(2) = 4e-049
Identities = 22/26 (84%), Positives = 24/26 (92%)
Frame = +1 / +3
Query: 766 VLTDTFHKPGFKLHVSVLQHLFCAVD 843
VLTDTFHKPGFKLHV VLQHLFC+ +
Sbjct: 3 VLTDTFHKPGFKLHVLVLQHLFCSAE 80
Score = 46.0 bits (94), Expect(2) = 3e-026
Identities = 19/26 (73%), Positives = 21/26 (80%)
Frame = -2 / -3
Query: 842 STAQNKCCSTET*SLKPGLWNVSVRT 765
S QNKCCST+T +LKPGL NVSV T
Sbjct: 79 SAEQNKCCSTKTCNLKPGL*NVSVNT 2
>gb|BU811752.1|BU811752 UL88TB08 Populus leaf cDNA library Populus tremula x Populus
tremuloides cDNA 5 prime, mRNA sequence
Length = 559
Score = 176 bits (378), Expect = 4e-043
Identities = 75/109 (68%), Positives = 87/109 (79%)
Frame = +1 / +1
Query: 871 SSVPYQYTDNAMFVRDYTIKLLGTSFPNMTVAEVTKFVDGLLSSKLDLPSFKNHIRDFLV 1050
++VPY Y +NAMFVR+YTIKLLGTSFPNMT +EVT+FV+GL SK +L FKNHIRDFLV
Sbjct: 1 TTVPYPYLNNAMFVREYTIKLLGTSFPNMTASEVTQFVNGLFESKNNLSIFKNHIRDFLV 180
Query: 1051 QSKEFSAQDNKDLYXXXXXXXXXXXXXXMLAIPGLIAPSELQDEMVDS* 1197
QSKEFSAQDNKDLY ML+IPGLIAP+E+QDEM+DS*
Sbjct: 181 QSKEFSAQDNKDLYAEEAAVQRERERQRMLSIPGLIAPNEIQDEMLDS* 327
Score = 85.8 bits (181), Expect(2) = 5e-027
Identities = 41/64 (64%), Positives = 45/64 (70%)
Frame = -2 / -3
Query: 1067 ENSFDCTRKSLIWFLKLGRSSFELRRPSTNLVTSATVMLGNDVPKSFMV*SRTNMALSVY 888
ENSFDCTRKS + FL + R F+ P TN VTS VMLGNDVPKS MV SRTN+AL Y
Sbjct: 197 ENSFDCTRKSRM*FLNMDRLFFDSNSPLTN*VTSDAVMLGNDVPKSLMVYSRTNIALFKY 18
Query: 887 *YGT 876
GT
Sbjct: 17 G*GT 6
Score = 56.5 bits (117), Expect(2) = 5e-027
Identities = 24/39 (61%), Positives = 29/39 (74%)
Frame = -1 / -2
Query: 1212 SKQGTLRIYHLVLQFARGNQPRNGKHSLALPFSLGSSLF 1096
+K +LRI HL+L F RGN+ N KHSL+LPF L SSLF
Sbjct: 342 AKNSSLRI*HLILYFIRGNKTWNRKHSLSLPFPLNSSLF 226
>gb|DT508840.1|DT508840 WS02422.BR_B07 PTxD-ICC-N-A-14 Populus trichocarpa x Populus
deltoides cDNA clone WS02422_B07 5', mRNA sequence
Length = 265
Score = 174 bits (374), Expect = 2e-042
Identities = 74/88 (84%), Positives = 82/88 (93%)
Frame = +1 / +2
Query: 520 LKFFSLLRAIGTHCFQALIQLSSQQLKLVIDSINWAFRHTERNIAETGLSLLLEILKKFQ 699
LKFFSLLRAI THCF ALI+LSS+QLKLV+DSI WAFRHTERNIAETGL+LL+E+LK FQ
Sbjct: 2 LKFFSLLRAIATHCFPALIRLSSEQLKLVMDSIIWAFRHTERNIAETGLNLLVEMLKNFQ 181
Query: 700 ASGFQNQFYKTYFLTIEQEIFAVLTDTF 783
AS F NQFY++YFLTIEQEIFAVLTDTF
Sbjct: 182 ASEFCNQFYRSYFLTIEQEIFAVLTDTF 265
Score = 115 bits (245), Expect = 1e-024
Identities = 57/87 (65%), Positives = 64/87 (73%)
Frame = -2 / -2
Query: 782 NVSVRTAKISCSIVKK*VL*NWFWNPEAWNFFKISNNRLRPVSAIFLSVCLNAQLIESIT 603
NVSV+TA ISCSIVKK L*N N EA FF IS NR RPVSA+FLSV NA +IESIT
Sbjct: 264 NVSVKTANISCSIVKKYDL*N*LQNSEA*KFFSISTNRFRPVSAMFLSV*RNAHMIESIT 85
Query: 602 SFNC*LESWINAWKQWVPIARSKEKNL 522
+F+C L+ I A KQ V +ARS EKNL
Sbjct: 84 NFSCSLDKRIKAGKQCVAMARSNEKNL 4
>gb|BU808966.1|BU808966 UL52PB01 Populus leaf cDNA library Populus tremula x Populus
tremuloides cDNA 5 prime, mRNA sequence
Length = 459
Score = 163 bits (350), Expect = 3e-039
Identities = 70/87 (80%), Positives = 82/87 (94%)
Frame = +1 / +1
Query: 7 SIDILKNQDVIRSVLNILQTNTSVASSLGPHFFPQISLIFLDMLTVYRMYSELVSSTIAE 186
S+D LK+Q+VIR+VLNILQTNTSVA+SLG +F Q+SLIFLDML VYRMYSEL+SS+IAE
Sbjct: 199 SVDFLKDQEVIRTVLNILQTNTSVANSLGTYFLSQMSLIFLDMLNVYRMYSELISSSIAE 378
Query: 187 GGPFASRTSFVKLLRSVKRETLKLIET 267
GGP+AS+TS+VKLLRSVKRETLKLIET
Sbjct: 379 GGPYASKTSYVKLLRSVKRETLKLIET 459
Score = 111 bits (236), Expect = 2e-023
Identities = 57/87 (65%), Positives = 64/87 (73%)
Frame = -2 / -2
Query: 266 VSINFRVSLLTDLKSFTNDVLEAKGPPSAIVLDTSSLYIL*TVSMSKKISEICGKKCGPS 87
VS++ VSLLTD KSFT DVL+A GPPSAI+LD SSLYIL T+SMSK IS+I KK P
Sbjct: 458 VSMSLSVSLLTDRKSFT*DVLDA*GPPSAILLDISSLYILYTLSMSKNISDI*DKKYVPR 279
Query: 86 ELATLVFVCRMLRTDRITSWFFRISIL 6
E ATLV VC +L T ITS FR S L
Sbjct: 278 EFATLVLVCNILSTVLITS*SFRKSTL 198
>gb|CA924195.1|CA924195 MTU7CL.P2.H03 Aspen leaf cDNA Library Populus tremuloides cDNA, mRNA
sequence
Length = 485
Score = 133 bits (286), Expect = 2e-030
Identities = 57/85 (67%), Positives = 66/85 (77%)
Frame = +1 / +2
Query: 943 SFPNMTVAEVTKFVDGLLSSKLDLPSFKNHIRDFLVQSKEFSAQDNKDLYXXXXXXXXXX 1122
SFPNMT +EVT+FV+GL S+ DL +FKNHIRDFLVQSKEFSAQDNKDLY
Sbjct: 2 SFPNMTASEVTQFVNGLFESRNDLSAFKNHIRDFLVQSKEFSAQDNKDLYAEEAAAQRER 181
Query: 1123 XXXXMLAIPGLIAPSELQDEMVDS* 1197
ML+IPGLIAP+E+QDEM+DS*
Sbjct: 182 ERQRMLSIPGLIAPNEIQDEMLDS* 256
>gb|CA924314.1|CA924314 MTU7CL.P4.C03 Aspen leaf cDNA Library Populus tremuloides cDNA, mRNA
sequence
Length = 485
Score = 133 bits (286), Expect = 2e-030
Identities = 57/85 (67%), Positives = 66/85 (77%)
Frame = +1 / +2
Query: 943 SFPNMTVAEVTKFVDGLLSSKLDLPSFKNHIRDFLVQSKEFSAQDNKDLYXXXXXXXXXX 1122
SFPNMT +EVT+FV+GL S+ DL +FKNHIRDFLVQSKEFSAQDNKDLY
Sbjct: 2 SFPNMTASEVTQFVNGLFESRNDLSAFKNHIRDFLVQSKEFSAQDNKDLYAEEAAAQRER 181
Query: 1123 XXXXMLAIPGLIAPSELQDEMVDS* 1197
ML+IPGLIAP+E+QDEM+DS*
Sbjct: 182 ERQRMLSIPGLIAPNEIQDEMLDS* 256
>gb|BU815823.1|BU815823 N047A09 Populus bark cDNA library Populus tremula x Populus
tremuloides cDNA 5 prime, mRNA sequence
Length = 204
Score = 74.8 bits (157), Expect(2) = 3e-025
Identities = 28/37 (75%), Positives = 32/37 (86%)
Frame = +1 / +3
Query: 847 LTEPLWDASSVPYQYTDNAMFVRDYTIKLLGTSFPNM 957
L EPLWD ++VPY Y +NAMFVR+YTIKLLGT FPNM
Sbjct: 93 LLEPLWDTTTVPYSYLNNAMFVREYTIKLLGTLFPNM 203
Score = 61.6 bits (128), Expect(2) = 3e-025
Identities = 23/27 (85%), Positives = 25/27 (92%)
Frame = +1 / +3
Query: 763 AVLTDTFHKPGFKLHVSVLQHLFCAVD 843
AVLTDTFHKPGFKLHV VLQHLFC+ +
Sbjct: 3 AVLTDTFHKPGFKLHVLVLQHLFCSAE 83
>gb|DN500454.1|DN500454 UL52PB01.5pR Populus cold stressed leaves cDNA library Populus
tremula x Populus tremuloides cDNA clone UL52PB01 5',
mRNA sequence
Length = 404
Score = 106 bits (227), Expect = 3e-022
Identities = 46/59 (77%), Positives = 54/59 (91%)
Frame = +1 / +2
Query: 7 SIDILKNQDVIRSVLNILQTNTSVASSLGPHFFPQISLIFLDMLTVYRMYSELVSSTIA 183
S+D LK+Q+VIR+VLNILQTNTSVA+SLG +F QISLIFLDML VYRMYSEL+SS+IA
Sbjct: 194 SVDFLKDQEVIRTVLNILQTNTSVANSLGTYFLSQISLIFLDMLNVYRMYSELISSSIA 370
Database: Populus_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:49 PM
Number of letters in database: 203,408,664
Number of sequences in database: 369,679
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 817,928,404
Number of Sequences: 369679
Number of extensions: 12216784
Number of successful extensions: 62339
Number of sequences better than 1.0e-020: 13
length of database: 67,802,888
effective HSP length: 54
effective length of database: 47,840,222
effective search space used: 22867626116
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)