TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QAF1g01.yg.2.1
(335 letters)
Database: Populus_nucl_with_EST.fasta
369,679 sequences; 203,408,664 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|DT497112.1|DT497112 WS01125.BR_I08 PT-P-FL-A-2 Populus t... 174 2e-043
gb|DT495294.1|DT495294 WS01120.BR_A19 PT-P-FL-A-2 Populus t... 107 4e-037
gb|AC149540.1| Populus trichocarpa clone Pop1-111J19, compl... 76 2e-036
gb|CA821982.1|CA821982 R02C12 two-month-old roots from clon... 141 1e-033
gb|AI166257.1|AI166257 xylem.est.107 Poplar xylem Lambda ZA... 122 1e-027
gb|DT493588.1|DT493588 WS0111.BR_N04 PT-P-FL-A-2 Populus tr... 109 8e-024
>gb|DT497112.1|DT497112 WS01125.BR_I08 PT-P-FL-A-2 Populus trichocarpa cDNA clone
WS01125_I08 5', mRNA sequence
Length = 908
Score = 174 bits (374), Expect = 2e-043
Identities = 67/88 (76%), Positives = 79/88 (89%)
Frame = -2 / +2
Query: 265 KVRXXYXXPDVFDRIFXITRGGIXKASRVINISEDIYAGFNSTLRQGNITHHEYIQVGKG 86
KVR Y PDVFDR+F +TRGG+ KAS+VIN+SEDI+AGFNSTLR+GN+THHEYIQVGKG
Sbjct: 437 KVRFHYGHPDVFDRLFHLTRGGVSKASKVINLSEDIFAGFNSTLREGNVTHHEYIQVGKG 616
Query: 85 RDVGLNQIALFEGKVAGGNGEQVLSRDV 2
RDVGLNQI++FE K+A GNGEQ LSRD+
Sbjct: 617 RDVGLNQISMFEAKIANGNGEQTLSRDI 700
>gb|DT495294.1|DT495294 WS01120.BR_A19 PT-P-FL-A-2 Populus trichocarpa cDNA clone
WS01120_A19 5', mRNA sequence
Length = 872
Score = 107 bits (229), Expect(2) = 4e-037
Identities = 42/64 (65%), Positives = 51/64 (79%)
Frame = -2 / +2
Query: 265 KVRXXYXXPDVFDRIFXITRGGIXKASRVINISEDIYAGFNSTLRQGNITHHEYIQVGKG 86
KVR Y PDVFDR+F +TRGG+ KAS+VIN+SEDI+AGFNSTLR+GN+THHE V +G
Sbjct: 434 KVRFHYGHPDVFDRLFHLTRGGVSKASKVINLSEDIFAGFNSTLREGNVTHHENXPVWEG 613
Query: 85 RDVG 74
G
Sbjct: 614 ERCG 625
Score = 65.7 bits (137), Expect(2) = 4e-037
Identities = 25/33 (75%), Positives = 29/33 (87%)
Frame = -2 / +3
Query: 100 QVGKGRDVGLNQIALFEGKVAGGNGEQVLSRDV 2
Q GKGRDVGLNQI++FE K+A GNGEQ LSRD+
Sbjct: 600 QFGKGRDVGLNQISMFEAKIANGNGEQTLSRDI 698
>gb|AC149540.1| Populus trichocarpa clone Pop1-111J19, complete sequence
Length = 96953
Score = 75.8 bits (159), Expect(3) = 2e-036
Identities = 31/44 (70%), Positives = 35/44 (79%)
Frame = -2 / +3
Query: 265 KVRXXYXXPDVFDRIFXITRGGIXKASRVINISEDIYAGFNSTL 134
+VR Y PDVFDR+F ITRGGI KASRVINISEDIY+G + L
Sbjct: 25269 RVRMHYGHPDVFDRVFHITRGGISKASRVINISEDIYSGMTALL 25400
Score = 74.8 bits (157), Expect(3) = 2e-036
Identities = 30/34 (88%), Positives = 34/34 (100%)
Frame = -2 / +1
Query: 103 IQVGKGRDVGLNQIALFEGKVAGGNGEQVLSRDV 2
++VGKGRDVGLNQIA+FEGKV+GGNGEQVLSRDV
Sbjct: 26011 LKVGKGRDVGLNQIAVFEGKVSGGNGEQVLSRDV 26112
Score = 52.4 bits (108), Expect(3) = 2e-036
Identities = 19/21 (90%), Positives = 21/21 (100%)
Frame = -2 / +2
Query: 157 YAGFNSTLRQGNITHHEYIQV 95
+AGFNSTLRQGN+THHEYIQV
Sbjct: 25886 HAGFNSTLRQGNVTHHEYIQV 25948
>gb|CA821982.1|CA821982 R02C12 two-month-old roots from clone 'Beaupre' Populus trichocarpa
x Populus deltoides cDNA 5', mRNA sequence
Length = 291
Score = 141 bits (303), Expect = 1e-033
Identities = 60/74 (81%), Positives = 64/74 (86%)
Frame = -2 / +2
Query: 223 IFXITRGGIXKASRVINISEDIYAGFNSTLRQGNITHHEYIQVGKGRDVGLNQIALFEGK 44
++ GI KASRVINISEDI+AGFN+TLRQG HEYIQVGKGRDVGLNQIALFEGK
Sbjct: 11 LYFYNSSGISKASRVINISEDIFAGFNTTLRQGKYYSHEYIQVGKGRDVGLNQIALFEGK 190
Query: 43 VAGGNGEQVLSRDV 2
VAGGNGEQVLSRDV
Sbjct: 191 VAGGNGEQVLSRDV 232
Score = 115 bits (245), Expect = 1e-025
Identities = 50/67 (74%), Positives = 50/67 (74%)
Frame = +3 / -1
Query: 3 TSRLRTCSPLPPATFPSNSAIWFKPTSRPFPTWIYSWCVMFPWRKVELNPAYISSLILIT 182
TS LRTCSP PPATFPS AIWF PTS PFPT IYSW FP V LNPA ISSLILIT
Sbjct: 231 TSLLRTCSPFPPATFPSKRAIWFNPTSLPFPT*IYSWE*YFPCLNVVLNPANISSLILIT 52
Query: 183 RDAXXMP 203
RDA MP
Sbjct: 51 RDALLMP 31
>gb|AI166257.1|AI166257 xylem.est.107 Poplar xylem Lambda ZAPII library Populus trichocarpa
cDNA 5', mRNA sequence
Length = 583
Score = 122 bits (260), Expect = 1e-027
Identities = 46/57 (80%), Positives = 54/57 (94%)
Frame = -2 / +2
Query: 172 ISEDIYAGFNSTLRQGNITHHEYIQVGKGRDVGLNQIALFEGKVAGGNGEQVLSRDV 2
+SEDI+AGFNSTLR+GN+THHEYIQVGKGRDVGLNQI++FE K+A GNGEQ LSRD+
Sbjct: 2 LSEDIFAGFNSTLREGNVTHHEYIQVGKGRDVGLNQISMFEAKIANGNGEQTLSRDI 172
>gb|DT493588.1|DT493588 WS0111.BR_N04 PT-P-FL-A-2 Populus trichocarpa cDNA clone WS0111_N04
5', mRNA sequence
Length = 599
Score = 109 bits (232), Expect = 8e-024
Identities = 41/55 (74%), Positives = 49/55 (89%)
Frame = -2 / +2
Query: 265 KVRXXYXXPDVFDRIFXITRGGIXKASRVINISEDIYAGFNSTLRQGNITHHEYI 101
KVR Y PDVFDR+F +TRGG+ KAS+VIN+SEDI+AGFNSTLR+GN+THHEYI
Sbjct: 434 KVRFHYGHPDVFDRLFHLTRGGVSKASKVINLSEDIFAGFNSTLREGNVTHHEYI 598
Database: Populus_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:49 PM
Number of letters in database: 203,408,664
Number of sequences in database: 369,679
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 120,689,594
Number of Sequences: 369679
Number of extensions: 1476679
Number of successful extensions: 100651
Number of sequences better than 1.0e-020: 7
length of database: 67,802,888
effective HSP length: 49
effective length of database: 49,688,617
effective search space used: 3080694254
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)