TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QAF14f01.yg.2.1
(319 letters)
Database: Populus_nucl_with_EST.fasta
369,679 sequences; 203,408,664 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CV257130.1|CV257130 WS0245.B21_M05 PTxD-ICC-N-A-14 Popul... 80 2e-026
gb|CV240226.1|CV240226 WS0234.B21_M19 PT-MB-A-13 Populus tr... 76 2e-025
gb|DT507435.1|DT507435 WS02418.BR_E03 PTxD-ICC-N-A-14 Popul... 76 2e-025
gb|CK097179.1|CK097179 UB42BPD03.3pR Populus active cambium... 76 2e-025
gb|DT512063.1|DT512063 WS02418.B21_E03 PTxD-ICC-N-A-14 Popu... 76 2e-025
gb|DT515203.1|DT515203 WS02426.B21_O22 PTxD-ICC-N-A-14 Popu... 76 3e-025
gb|CV257505.1|CV257505 WS0246.B21_N16 PTxD-ICC-N-A-14 Popul... 73 1e-024
gb|CK091185.1|CK091185 F056P93.3pR Populus flower cDNA libr... 74 2e-024
gb|CX656255.1|CX656255 PO02051A03 Poplar SC cDNA library Po... 77 5e-024
gb|DT507569.1|DT507569 WS02418.BR_J21 PTxD-ICC-N-A-14 Popul... 67 1e-022
gb|DT512195.1|DT512195 WS02418.B21_J21 PTxD-ICC-N-A-14 Popu... 67 1e-022
gb|CK088858.1|CK088858 A076P21.3pR Hybrid aspen plasmid lib... 57 4e-021
>gb|CV257130.1|CV257130 WS0245.B21_M05 PTxD-ICC-N-A-14 Populus trichocarpa x Populus
deltoides cDNA clone WS0245_M05 3', mRNA sequence
Length = 718
Score = 80.3 bits (169), Expect(2) = 2e-026
Identities = 26/54 (48%), Positives = 37/54 (68%)
Frame = -3 / -3
Query: 317 YLXXXXPIVRDRFNPNACAWAYGVNVFXLXXXXRDGCTELFHQYMEMNEDXELW 156
YL P++++RFNP ACAWA+G+N+F L R+ CTE +H + +NED LW
Sbjct: 623 YLNFSHPLIKERFNPKACAWAFGMNIFDLDAWRREKCTEHYHYWQSLNEDRTLW 462
Score = 56.1 bits (116), Expect(2) = 2e-026
Identities = 23/40 (57%), Positives = 26/40 (65%)
Frame = -3 / -3
Query: 140 LTAGXXXXXGNTKXXDKSWHVMGLGYNPSISPEAIRSAAV 21
L G TK DKSWHV+GLGYNPSIS + I +AAV
Sbjct: 449 LPPGLITFYSTTKSLDKSWHVLGLGYNPSISMDEISNAAV 330
>gb|CV240226.1|CV240226 WS0234.B21_M19 PT-MB-A-13 Populus trichocarpa cDNA clone WS0234_M19
3', mRNA sequence
Length = 733
Score = 75.8 bits (159), Expect(2) = 2e-025
Identities = 24/54 (44%), Positives = 36/54 (66%)
Frame = -3 / -2
Query: 317 YLXXXXPIVRDRFNPNACAWAYGVNVFXLXXXXRDGCTELFHQYMEMNEDXELW 156
Y+ P+++++FNP ACAWAYG+N F L R+ CTE +H + +NE+ LW
Sbjct: 684 YMNFSHPLIKEKFNPKACAWAYGMNFFDLDAWRREKCTEEYHYWQNLNENRTLW 523
Score = 57.4 bits (119), Expect(2) = 2e-025
Identities = 24/40 (60%), Positives = 27/40 (67%)
Frame = -3 / -2
Query: 140 LTAGXXXXXGNTKXXDKSWHVMGLGYNPSISPEAIRSAAV 21
L G TK DKSWHV+GLGYNPSIS + I+SAAV
Sbjct: 510 LPPGLITFYSTTKPLDKSWHVLGLGYNPSISMDEIQSAAV 391
>gb|DT507435.1|DT507435 WS02418.BR_E03 PTxD-ICC-N-A-14 Populus trichocarpa x Populus
deltoides cDNA clone WS02418_E03 5', mRNA sequence
Length = 735
Score = 75.8 bits (159), Expect(2) = 2e-025
Identities = 24/54 (44%), Positives = 36/54 (66%)
Frame = -3 / +1
Query: 317 YLXXXXPIVRDRFNPNACAWAYGVNVFXLXXXXRDGCTELFHQYMEMNEDXELW 156
Y+ P+++++FNP ACAWAYG+N F L R+ CTE +H + +NE+ LW
Sbjct: 55 YMNFSHPLIKEKFNPKACAWAYGMNFFDLDAWRREKCTEEYHYWQNLNENRTLW 216
Score = 57.4 bits (119), Expect(2) = 2e-025
Identities = 24/40 (60%), Positives = 27/40 (67%)
Frame = -3 / +1
Query: 140 LTAGXXXXXGNTKXXDKSWHVMGLGYNPSISPEAIRSAAV 21
L G TK DKSWHV+GLGYNPSIS + I+SAAV
Sbjct: 229 LPPGLITFYSTTKPLDKSWHVLGLGYNPSISMDEIQSAAV 348
>gb|CK097179.1|CK097179 UB42BPD03.3pR Populus active cambium cDNA library Populus tremula
cDNA clone UB42BPD03 3', mRNA sequence
Length = 740
Score = 75.8 bits (159), Expect(2) = 2e-025
Identities = 24/54 (44%), Positives = 36/54 (66%)
Frame = -3 / -2
Query: 317 YLXXXXPIVRDRFNPNACAWAYGVNVFXLXXXXRDGCTELFHQYMEMNEDXELW 156
Y+ P+++++FNP ACAWAYG+N F L R+ CTE +H + +NE+ LW
Sbjct: 625 YMNFSHPLIKEKFNPKACAWAYGMNFFDLDAWRREKCTEEYHYWQNLNENRTLW 464
Score = 57.4 bits (119), Expect(2) = 2e-025
Identities = 24/40 (60%), Positives = 27/40 (67%)
Frame = -3 / -2
Query: 140 LTAGXXXXXGNTKXXDKSWHVMGLGYNPSISPEAIRSAAV 21
L G TK DKSWHV+GLGYNPSIS + I+SAAV
Sbjct: 451 LPPGLITFYSTTKPLDKSWHVLGLGYNPSISMDEIQSAAV 332
>gb|DT512063.1|DT512063 WS02418.B21_E03 PTxD-ICC-N-A-14 Populus trichocarpa x Populus
deltoides cDNA clone WS02418_E03 3', mRNA sequence
Length = 748
Score = 75.8 bits (159), Expect(2) = 2e-025
Identities = 24/54 (44%), Positives = 36/54 (66%)
Frame = -3 / -1
Query: 317 YLXXXXPIVRDRFNPNACAWAYGVNVFXLXXXXRDGCTELFHQYMEMNEDXELW 156
Y+ P+++++FNP ACAWAYG+N F L R+ CTE +H + +NE+ LW
Sbjct: 694 YMNFSHPLIKEKFNPKACAWAYGMNFFDLDAWRREKCTEEYHYWQNLNENRTLW 533
Score = 57.4 bits (119), Expect(2) = 2e-025
Identities = 24/40 (60%), Positives = 27/40 (67%)
Frame = -3 / -1
Query: 140 LTAGXXXXXGNTKXXDKSWHVMGLGYNPSISPEAIRSAAV 21
L G TK DKSWHV+GLGYNPSIS + I+SAAV
Sbjct: 520 LPPGLITFYSTTKPLDKSWHVLGLGYNPSISMDEIQSAAV 401
>gb|DT515203.1|DT515203 WS02426.B21_O22 PTxD-ICC-N-A-14 Populus trichocarpa x Populus
deltoides cDNA clone WS02426_O22 3', mRNA sequence
Length = 918
Score = 75.8 bits (159), Expect(2) = 3e-025
Identities = 24/54 (44%), Positives = 36/54 (66%)
Frame = -3 / -3
Query: 317 YLXXXXPIVRDRFNPNACAWAYGVNVFXLXXXXRDGCTELFHQYMEMNEDXELW 156
Y+ P+++++FNP ACAWAYG+N F L R+ CTE +H + +NE+ LW
Sbjct: 694 YMNFSHPLIKEKFNPKACAWAYGMNFFDLDAWRREKCTEEYHYWQNLNENRTLW 533
Score = 57.4 bits (119), Expect(2) = 3e-025
Identities = 24/40 (60%), Positives = 27/40 (67%)
Frame = -3 / -3
Query: 140 LTAGXXXXXGNTKXXDKSWHVMGLGYNPSISPEAIRSAAV 21
L G TK DKSWHV+GLGYNPSIS + I+SAAV
Sbjct: 520 LPPGLITFYSTTKPLDKSWHVLGLGYNPSISMDEIQSAAV 401
>gb|CV257505.1|CV257505 WS0246.B21_N16 PTxD-ICC-N-A-14 Populus trichocarpa x Populus
deltoides cDNA clone WS0246_N16 3', mRNA sequence
Length = 691
Score = 73.0 bits (153), Expect(2) = 1e-024
Identities = 23/48 (47%), Positives = 34/48 (70%)
Frame = -3 / -3
Query: 299 PIVRDRFNPNACAWAYGVNVFXLXXXXRDGCTELFHQYMEMNEDXELW 156
P+++++FNP ACAWAYG+N F L R+ CTE +H + +NE+ LW
Sbjct: 677 PLIKEKFNPKACAWAYGMNFFDLDAWRREKCTEEYHYWQNLNENRTLW 534
Score = 57.4 bits (119), Expect(2) = 1e-024
Identities = 24/40 (60%), Positives = 27/40 (67%)
Frame = -3 / -3
Query: 140 LTAGXXXXXGNTKXXDKSWHVMGLGYNPSISPEAIRSAAV 21
L G TK DKSWHV+GLGYNPSIS + I+SAAV
Sbjct: 521 LPPGLITFYSTTKPLDKSWHVLGLGYNPSISMDEIQSAAV 402
>gb|CK091185.1|CK091185 F056P93.3pR Populus flower cDNA library Populus trichocarpa cDNA
clone F056P93 3', mRNA sequence
Length = 724
Score = 73.9 bits (155), Expect(2) = 2e-024
Identities = 23/48 (47%), Positives = 34/48 (70%)
Frame = -3 / -3
Query: 299 PIVRDRFNPNACAWAYGVNVFXLXXXXRDGCTELFHQYMEMNEDXELW 156
P++++RF P ACAWA+G+N+F L R+ CTE +H + +NED LW
Sbjct: 680 PLIKERFTPKACAWAFGMNIFDLDAWRREKCTEHYHYWQSLNEDRTLW 537
Score = 56.1 bits (116), Expect(2) = 2e-024
Identities = 23/40 (57%), Positives = 26/40 (65%)
Frame = -3 / -3
Query: 140 LTAGXXXXXGNTKXXDKSWHVMGLGYNPSISPEAIRSAAV 21
L G TK DKSWHV+GLGYNPSIS + I +AAV
Sbjct: 524 LPPGLITFYSTTKSLDKSWHVLGLGYNPSISMDEISNAAV 405
>gb|CX656255.1|CX656255 PO02051A03 Poplar SC cDNA library Populus alba x Populus tremula
var. glandulosa cDNA clone PO02051A03 5', mRNA sequence
Length = 297
Score = 77.1 bits (162), Expect(2) = 5e-024
Identities = 25/54 (46%), Positives = 36/54 (66%)
Frame = -3 / +2
Query: 317 YLXXXXPIVRDRFNPNACAWAYGVNVFXLXXXXRDGCTELFHQYMEMNEDXELW 156
YL P++++ FNP ACAWA+G+N+F L R+ CTE +H + +NED LW
Sbjct: 5 YLNFSHPLIKEGFNPKACAWAFGMNIFDLDAWRREKCTEHYHYWQSLNEDRTLW 166
Score = 50.1 bits (103), Expect(2) = 5e-024
Identities = 20/39 (51%), Positives = 24/39 (61%)
Frame = -3 / +2
Query: 140 LTAGXXXXXGNTKXXDKSWHVMGLGYNPSISPEAIRSAA 24
L G K DKSWHV+GLGYNPSI+ + I +AA
Sbjct: 179 LPPGLITFYSTKKSLDKSWHVLGLGYNPSITMDEISNAA 295
>gb|DT507569.1|DT507569 WS02418.BR_J21 PTxD-ICC-N-A-14 Populus trichocarpa x Populus
deltoides cDNA clone WS02418_J21 5', mRNA sequence
Length = 665
Score = 66.6 bits (139), Expect(2) = 1e-022
Identities = 22/44 (50%), Positives = 30/44 (68%)
Frame = -3 / +2
Query: 287 DRFNPNACAWAYGVNVFXLXXXXRDGCTELFHQYMEMNEDXELW 156
++FNP ACAWAYG+N F L R+ CTE +H + +NE+ LW
Sbjct: 2 EKFNPKACAWAYGMNFFDLDAWRREKCTEEYHYWQNLNENRTLW 133
Score = 57.4 bits (119), Expect(2) = 1e-022
Identities = 24/40 (60%), Positives = 27/40 (67%)
Frame = -3 / +2
Query: 140 LTAGXXXXXGNTKXXDKSWHVMGLGYNPSISPEAIRSAAV 21
L G TK DKSWHV+GLGYNPSIS + I+SAAV
Sbjct: 146 LPPGLITFYSTTKPLDKSWHVLGLGYNPSISMDEIQSAAV 265
>gb|DT512195.1|DT512195 WS02418.B21_J21 PTxD-ICC-N-A-14 Populus trichocarpa x Populus
deltoides cDNA clone WS02418_J21 3', mRNA sequence
Length = 665
Score = 66.6 bits (139), Expect(2) = 1e-022
Identities = 22/44 (50%), Positives = 30/44 (68%)
Frame = -3 / -2
Query: 287 DRFNPNACAWAYGVNVFXLXXXXRDGCTELFHQYMEMNEDXELW 156
++FNP ACAWAYG+N F L R+ CTE +H + +NE+ LW
Sbjct: 664 EKFNPKACAWAYGMNFFDLDAWRREKCTEEYHYWQNLNENRTLW 533
Score = 57.4 bits (119), Expect(2) = 1e-022
Identities = 24/40 (60%), Positives = 27/40 (67%)
Frame = -3 / -2
Query: 140 LTAGXXXXXGNTKXXDKSWHVMGLGYNPSISPEAIRSAAV 21
L G TK DKSWHV+GLGYNPSIS + I+SAAV
Sbjct: 520 LPPGLITFYSTTKPLDKSWHVLGLGYNPSISMDEIQSAAV 401
>gb|CK088858.1|CK088858 A076P21.3pR Hybrid aspen plasmid library Populus tremula x Populus
tremuloides cDNA clone A076P21 3', mRNA sequence
Length = 708
Score = 57.4 bits (119), Expect(3) = 4e-021
Identities = 24/40 (60%), Positives = 27/40 (67%)
Frame = -3 / -2
Query: 140 LTAGXXXXXGNTKXXDKSWHVMGLGYNPSISPEAIRSAAV 21
L G TK DKSWHV+GLGYNPSIS + I+SAAV
Sbjct: 536 LPPGLITFYSTTKPLDKSWHVLGLGYNPSISMDEIQSAAV 417
Score = 47.3 bits (97), Expect(3) = 4e-021
Identities = 14/23 (60%), Positives = 20/23 (86%)
Frame = -3 / -3
Query: 299 PIVRDRFNPNACAWAYGVNVFXL 231
P+++++FNP ACAWAYG+N F L
Sbjct: 691 PLIKEKFNPKACAWAYGMNFFDL 623
Score = 30.4 bits (60), Expect(3) = 4e-021
Identities = 9/21 (42%), Positives = 14/21 (66%)
Frame = -3 / -2
Query: 218 RDGCTELFHQYMEMNEDXELW 156
R+ CTE +H + +NE+ LW
Sbjct: 611 REKCTEEYHYWQNLNENRTLW 549
Database: Populus_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:49 PM
Number of letters in database: 203,408,664
Number of sequences in database: 369,679
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 141,495,595
Number of Sequences: 369679
Number of extensions: 1495405
Number of successful extensions: 98267
Number of sequences better than 1.0e-020: 12
length of database: 67,802,888
effective HSP length: 49
effective length of database: 49,688,617
effective search space used: 2782562552
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)