TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MAD56_a5f4.3.2
(1520 letters)
Database: Populus_nucl_with_EST.fasta
369,679 sequences; 203,408,664 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|DT473549.1|DT473549 WS01229.BR_P18 PT-GT-FL-A-3 Populus ... 47 5e-024
gb|BU816746.1|BU816746 N070H04 Populus bark cDNA library Po... 61 3e-022
gb|BU814474.1|BU814474 N030aB12 Populus bark cDNA library P... 61 4e-022
gb|CX177063.1|CX177063 C02_69-49_06.ab1 leaf inoculated wit... 61 4e-022
gb|CX169858.1|CX169858 B08_69-97_04.ab1 leaf inoculated wit... 61 6e-022
gb|CK109852.1|CK109852 N030B12 Populus bark cDNA library Po... 61 6e-022
gb|CN524051.1|CN524051 GQ015M07.T3_H11 GQ015 Populus tricho... 61 6e-022
gb|DT503992.1|DT503992 WS0138.BR_J05 PTxD-IL-FL-A-4 Populus... 61 1e-021
gb|DT501968.1|DT501968 WS01314.BR_O23 PTxD-IL-FL-A-4 Populu... 61 1e-021
gb|BU814555.1|BU814555 N030bB12 Populus bark cDNA library P... 61 3e-021
>gb|DT473549.1|DT473549 WS01229.BR_P18 PT-GT-FL-A-3 Populus trichocarpa cDNA clone
WS01229_P18 5', mRNA sequence
Length = 954
Score = 47.3 bits (97), Expect(5) = 5e-024
Identities = 20/40 (50%), Positives = 23/40 (57%)
Frame = +3 / +1
Query: 270 VPLMFVFGDSLVDVGNNNFLPPPAPRAASPYGIDFPSGTP 389
VP + FGDS VDVGNN++LP PYG DF P
Sbjct: 97 VPAIITFGDSAVDVGNNDYLPTIFKANYPPYGRDFVDQKP 216
Score = 44.1 bits (90), Expect(5) = 5e-024
Identities = 15/39 (38%), Positives = 24/39 (61%)
Frame = +3 / +1
Query: 756 YISSMVSTYVQHIDALYKLGARRLGILDVPAIGCTPGSR 872
Y S +V ++ + LY LG R+LG+ +P +GC P +R
Sbjct: 580 YGSYLVGSFTSFVKTLYGLGGRKLGVTSLPPLGCLPAAR 696
Score = 38.2 bits (77), Expect(5) = 5e-024
Identities = 16/29 (55%), Positives = 18/29 (62%)
Frame = +3 / +1
Query: 399 SGRFTNGYNLADLVARRLGFKMSPPAYLS 485
+GRF NG D+ A LGFK PAYLS
Sbjct: 217 TGRFCNGKLATDITAETLGFKSYAPAYLS 303
Score = 37.7 bits (76), Expect(5) = 5e-024
Identities = 15/57 (26%), Positives = 27/57 (47%)
Frame = +3 / +1
Query: 954 AKAVASSMPGMRYSIASTYNFVTDLMDSHLVAGLRVVDRACCGSGKLNAAVMCAQPN 1124
A ++ +PG++ I + + DL+ S G + R CCG+G + + PN
Sbjct: 781 ATSLQKQLPGLKIVIFDIFQPLYDLVKSPSENGFQEARRGCCGTGTVETTSLLCNPN 951
Score = 25.4 bits (49), Expect(5) = 5e-024
Identities = 8/20 (40%), Positives = 16/20 (80%)
Frame = +3 / +1
Query: 651 KLSSMVSRSLFLISAGGNDF 710
K +S++ +L+++SAG +DF
Sbjct: 466 KSASIIKGALYILSAGSSDF 525
>gb|BU816746.1|BU816746 N070H04 Populus bark cDNA library Populus tremula x Populus
tremuloides cDNA 5 prime, mRNA sequence
Length = 667
Score = 60.6 bits (126), Expect(4) = 3e-022
Identities = 25/44 (56%), Positives = 27/44 (61%)
Frame = +3 / +3
Query: 270 VPLMFVFGDSLVDVGNNNFLPPPAPRAASPYGIDFPSGTPGAVS 401
VP FVFGDSL D GNNN+L P PYG+DFP G G S
Sbjct: 9 VPCYFVFGDSLFDNGNNNYLTTPVKVNYLPYGVDFPLGATGRCS 140
Score = 37.7 bits (76), Expect(4) = 3e-022
Identities = 11/39 (28%), Positives = 22/39 (56%)
Frame = +3 / +3
Query: 756 YISSMVSTYVQHIDALYKLGARRLGILDVPAIGCTPGSR 872
Y ++ TY ++ LY GAR++ + + +GC P ++
Sbjct: 495 YAQLLIKTYKTQLEELYSAGARKIAVFGLIRVGCMPSNK 611
Score = 35.9 bits (72), Expect(4) = 3e-022
Identities = 10/14 (71%), Positives = 11/14 (78%)
Frame = +2 / +2
Query: 521 PNWCQLRVWWIWHS 562
P WCQLR+ W WHS
Sbjct: 230 PRWCQLRI*WSWHS 271
Score = 33.1 bits (66), Expect(4) = 3e-022
Identities = 12/24 (50%), Positives = 17/24 (70%)
Frame = +3 / +3
Query: 387 PGAVSGRFTNGYNLADLVARRLGF 458
P +GR +NG N+AD +A +LGF
Sbjct: 114 PLGATGRCSNGLNIADTIAEQLGF 185
>gb|BU814474.1|BU814474 N030aB12 Populus bark cDNA library Populus tremula x Populus
tremuloides cDNA 5 prime, mRNA sequence
Length = 744
Score = 60.6 bits (126), Expect(4) = 4e-022
Identities = 25/44 (56%), Positives = 27/44 (61%)
Frame = +3 / +2
Query: 270 VPLMFVFGDSLVDVGNNNFLPPPAPRAASPYGIDFPSGTPGAVS 401
VP FVFGDSL D GNNN+L P PYG+DFP G G S
Sbjct: 29 VPCYFVFGDSLFDNGNNNYLTTPVKVNYLPYGVDFPLGATGRCS 160
Score = 37.7 bits (76), Expect(4) = 4e-022
Identities = 11/39 (28%), Positives = 22/39 (56%)
Frame = +3 / +2
Query: 756 YISSMVSTYVQHIDALYKLGARRLGILDVPAIGCTPGSR 872
Y ++ TY ++ LY GAR++ + + +GC P ++
Sbjct: 515 YAQLLIKTYKTQLEELYSAGARKIAVFGLIRVGCMPSNK 631
Score = 35.9 bits (72), Expect(4) = 4e-022
Identities = 10/14 (71%), Positives = 11/14 (78%)
Frame = +2 / +1
Query: 521 PNWCQLRVWWIWHS 562
P WCQLR+ W WHS
Sbjct: 250 PRWCQLRI*WSWHS 291
Score = 33.1 bits (66), Expect(4) = 4e-022
Identities = 12/24 (50%), Positives = 17/24 (70%)
Frame = +3 / +2
Query: 387 PGAVSGRFTNGYNLADLVARRLGF 458
P +GR +NG N+AD +A +LGF
Sbjct: 134 PLGATGRCSNGLNIADTIAEQLGF 205
>gb|CX177063.1|CX177063 C02_69-49_06.ab1 leaf inoculated with Marssonia pathogen of Populus
deltoides Populus deltoides cDNA, mRNA sequence
Length = 723
Score = 61.1 bits (127), Expect(4) = 4e-022
Identities = 26/46 (56%), Positives = 28/46 (60%)
Frame = +3 / +1
Query: 264 SSVPLMFVFGDSLVDVGNNNFLPPPAPRAASPYGIDFPSGTPGAVS 401
+ VP FVFGDSL D GNNN+L P PYGIDFP G G S
Sbjct: 25 AQVPCYFVFGDSLFDNGNNNYLNTPVKVNYLPYGIDFPLGATGRCS 162
Score = 36.8 bits (74), Expect(4) = 4e-022
Identities = 11/38 (28%), Positives = 21/38 (55%)
Frame = +3 / +1
Query: 756 YISSMVSTYVQHIDALYKLGARRLGILDVPAIGCTPGS 869
Y ++ TY ++ LY GAR++ + + +GC P +
Sbjct: 517 YAKHLIKTYKTQLEDLYSTGARKIAVFGLIRVGCMPSN 630
Score = 35.9 bits (72), Expect(4) = 4e-022
Identities = 10/14 (71%), Positives = 11/14 (78%)
Frame = +2 / +3
Query: 521 PNWCQLRVWWIWHS 562
P WCQLR+ W WHS
Sbjct: 252 PRWCQLRI*WSWHS 293
Score = 33.1 bits (66), Expect(4) = 4e-022
Identities = 12/24 (50%), Positives = 17/24 (70%)
Frame = +3 / +1
Query: 387 PGAVSGRFTNGYNLADLVARRLGF 458
P +GR +NG N+AD +A +LGF
Sbjct: 136 PLGATGRCSNGLNIADTIAEQLGF 207
>gb|CX169858.1|CX169858 B08_69-97_04.ab1 leaf inoculated with Marssonia pathogen of Populus
deltoides Populus deltoides cDNA, mRNA sequence
Length = 709
Score = 60.6 bits (126), Expect(4) = 6e-022
Identities = 26/44 (59%), Positives = 27/44 (61%)
Frame = +3 / +2
Query: 270 VPLMFVFGDSLVDVGNNNFLPPPAPRAASPYGIDFPSGTPGAVS 401
VP FVFGDSL D GNNN+L P PYGIDFP G G S
Sbjct: 50 VPCYFVFGDSLFDNGNNNYLNTPVKVNYLPYGIDFPLGATGRCS 181
Score = 36.8 bits (74), Expect(4) = 6e-022
Identities = 11/38 (28%), Positives = 21/38 (55%)
Frame = +3 / +2
Query: 756 YISSMVSTYVQHIDALYKLGARRLGILDVPAIGCTPGS 869
Y ++ TY ++ LY GAR++ + + +GC P +
Sbjct: 536 YAKHLIKTYKTQLEDLYSTGARKIAVFGLIRVGCMPSN 649
Score = 35.9 bits (72), Expect(4) = 6e-022
Identities = 10/14 (71%), Positives = 11/14 (78%)
Frame = +2 / +1
Query: 521 PNWCQLRVWWIWHS 562
P WCQLR+ W WHS
Sbjct: 271 PRWCQLRI*WSWHS 312
Score = 33.1 bits (66), Expect(4) = 6e-022
Identities = 12/24 (50%), Positives = 17/24 (70%)
Frame = +3 / +2
Query: 387 PGAVSGRFTNGYNLADLVARRLGF 458
P +GR +NG N+AD +A +LGF
Sbjct: 155 PLGATGRCSNGLNIADTIAEQLGF 226
>gb|CK109852.1|CK109852 N030B12 Populus bark cDNA library Populus tremula x Populus
tremuloides cDNA clone N030B12 5', mRNA sequence
Length = 868
Score = 60.6 bits (126), Expect(4) = 6e-022
Identities = 25/44 (56%), Positives = 27/44 (61%)
Frame = +3 / +1
Query: 270 VPLMFVFGDSLVDVGNNNFLPPPAPRAASPYGIDFPSGTPGAVS 401
VP FVFGDSL D GNNN+L P PYG+DFP G G S
Sbjct: 28 VPCYFVFGDSLFDNGNNNYLTTPVKVNYLPYGVDFPLGATGRCS 159
Score = 37.7 bits (76), Expect(4) = 6e-022
Identities = 11/39 (28%), Positives = 22/39 (56%)
Frame = +3 / +1
Query: 756 YISSMVSTYVQHIDALYKLGARRLGILDVPAIGCTPGSR 872
Y ++ TY ++ LY GAR++ + + +GC P ++
Sbjct: 514 YAQLLIKTYKTQLEELYSAGARKIAVFGLIRVGCMPSNK 630
Score = 35.9 bits (72), Expect(4) = 6e-022
Identities = 10/14 (71%), Positives = 11/14 (78%)
Frame = +2 / +3
Query: 521 PNWCQLRVWWIWHS 562
P WCQLR+ W WHS
Sbjct: 249 PRWCQLRI*WSWHS 290
Score = 33.1 bits (66), Expect(4) = 6e-022
Identities = 12/24 (50%), Positives = 17/24 (70%)
Frame = +3 / +1
Query: 387 PGAVSGRFTNGYNLADLVARRLGF 458
P +GR +NG N+AD +A +LGF
Sbjct: 133 PLGATGRCSNGLNIADTIAEQLGF 204
>gb|CN524051.1|CN524051 GQ015M07.T3_H11 GQ015 Populus trichocarpa x Populus deltoides cDNA
clone GQ015M07_H11 5', mRNA sequence
Length = 743
Score = 60.6 bits (126), Expect(4) = 6e-022
Identities = 26/44 (59%), Positives = 27/44 (61%)
Frame = +3 / +2
Query: 270 VPLMFVFGDSLVDVGNNNFLPPPAPRAASPYGIDFPSGTPGAVS 401
VP FVFGDSL D GNNN+L P PYGIDFP G G S
Sbjct: 8 VPCYFVFGDSLFDNGNNNYLNTPVKVNYLPYGIDFPLGATGRCS 139
Score = 36.8 bits (74), Expect(4) = 6e-022
Identities = 11/38 (28%), Positives = 21/38 (55%)
Frame = +3 / +2
Query: 756 YISSMVSTYVQHIDALYKLGARRLGILDVPAIGCTPGS 869
Y ++ TY ++ LY GAR++ + + +GC P +
Sbjct: 494 YAKHLIKTYKTQLEDLYSTGARKIAVFGLIRVGCMPSN 607
Score = 35.9 bits (72), Expect(4) = 6e-022
Identities = 10/14 (71%), Positives = 11/14 (78%)
Frame = +2 / +1
Query: 521 PNWCQLRVWWIWHS 562
P WCQLR+ W WHS
Sbjct: 229 PRWCQLRI*WSWHS 270
Score = 33.1 bits (66), Expect(4) = 6e-022
Identities = 12/24 (50%), Positives = 17/24 (70%)
Frame = +3 / +2
Query: 387 PGAVSGRFTNGYNLADLVARRLGF 458
P +GR +NG N+AD +A +LGF
Sbjct: 113 PLGATGRCSNGLNIADTIAEQLGF 184
>gb|DT503992.1|DT503992 WS0138.BR_J05 PTxD-IL-FL-A-4 Populus trichocarpa x Populus
deltoides cDNA clone WS0138_J05 5', mRNA sequence
Length = 861
Score = 60.6 bits (126), Expect(4) = 1e-021
Identities = 26/44 (59%), Positives = 27/44 (61%)
Frame = +3 / +2
Query: 270 VPLMFVFGDSLVDVGNNNFLPPPAPRAASPYGIDFPSGTPGAVS 401
VP FVFGDSL D GNNN+L P PYGIDFP G G S
Sbjct: 179 VPCYFVFGDSLFDNGNNNYLNTPVKVNYLPYGIDFPLGATGRCS 310
Score = 36.8 bits (74), Expect(4) = 1e-021
Identities = 11/38 (28%), Positives = 21/38 (55%)
Frame = +3 / +2
Query: 756 YISSMVSTYVQHIDALYKLGARRLGILDVPAIGCTPGS 869
Y ++ TY ++ LY GAR++ + + +GC P +
Sbjct: 665 YAKHLIKTYKTQLEDLYSTGARKIAVFGLIRVGCMPSN 778
Score = 35.9 bits (72), Expect(4) = 1e-021
Identities = 10/14 (71%), Positives = 11/14 (78%)
Frame = +2 / +1
Query: 521 PNWCQLRVWWIWHS 562
P WCQLR+ W WHS
Sbjct: 400 PRWCQLRI*WSWHS 441
Score = 33.1 bits (66), Expect(4) = 1e-021
Identities = 12/24 (50%), Positives = 17/24 (70%)
Frame = +3 / +2
Query: 387 PGAVSGRFTNGYNLADLVARRLGF 458
P +GR +NG N+AD +A +LGF
Sbjct: 284 PLGATGRCSNGLNIADTIAEQLGF 355
>gb|DT501968.1|DT501968 WS01314.BR_O23 PTxD-IL-FL-A-4 Populus trichocarpa x Populus
deltoides cDNA clone WS01314_O23 5', mRNA sequence
Length = 921
Score = 60.6 bits (126), Expect(4) = 1e-021
Identities = 26/44 (59%), Positives = 27/44 (61%)
Frame = +3 / +2
Query: 270 VPLMFVFGDSLVDVGNNNFLPPPAPRAASPYGIDFPSGTPGAVS 401
VP FVFGDSL D GNNN+L P PYGIDFP G G S
Sbjct: 179 VPCYFVFGDSLFDNGNNNYLNTPVKVNYLPYGIDFPLGATGRCS 310
Score = 36.8 bits (74), Expect(4) = 1e-021
Identities = 11/38 (28%), Positives = 21/38 (55%)
Frame = +3 / +2
Query: 756 YISSMVSTYVQHIDALYKLGARRLGILDVPAIGCTPGS 869
Y ++ TY ++ LY GAR++ + + +GC P +
Sbjct: 665 YAKHLIKTYKTQLEDLYSTGARKIAVFGLIRVGCMPSN 778
Score = 35.9 bits (72), Expect(4) = 1e-021
Identities = 10/14 (71%), Positives = 11/14 (78%)
Frame = +2 / +1
Query: 521 PNWCQLRVWWIWHS 562
P WCQLR+ W WHS
Sbjct: 400 PRWCQLRI*WSWHS 441
Score = 33.1 bits (66), Expect(4) = 1e-021
Identities = 12/24 (50%), Positives = 17/24 (70%)
Frame = +3 / +2
Query: 387 PGAVSGRFTNGYNLADLVARRLGF 458
P +GR +NG N+AD +A +LGF
Sbjct: 284 PLGATGRCSNGLNIADTIAEQLGF 355
>gb|BU814555.1|BU814555 N030bB12 Populus bark cDNA library Populus tremula x Populus
tremuloides cDNA 5 prime, mRNA sequence
Length = 597
Score = 60.6 bits (126), Expect(4) = 3e-021
Identities = 25/44 (56%), Positives = 27/44 (61%)
Frame = +3 / +3
Query: 270 VPLMFVFGDSLVDVGNNNFLPPPAPRAASPYGIDFPSGTPGAVS 401
VP FVFGDSL D GNNN+L P PYG+DFP G G S
Sbjct: 9 VPCYFVFGDSLFDNGNNNYLTTPVKVNYLPYGVDFPLGATGRCS 140
Score = 35.9 bits (72), Expect(4) = 3e-021
Identities = 10/14 (71%), Positives = 11/14 (78%)
Frame = +2 / +2
Query: 521 PNWCQLRVWWIWHS 562
P WCQLR+ W WHS
Sbjct: 230 PRWCQLRI*WSWHS 271
Score = 33.6 bits (67), Expect(4) = 3e-021
Identities = 10/34 (29%), Positives = 19/34 (55%)
Frame = +3 / +3
Query: 756 YISSMVSTYVQHIDALYKLGARRLGILDVPAIGC 857
Y ++ TY ++ LY GAR++ + + +GC
Sbjct: 495 YAQLLIKTYKTQLEELYSAGARKIAVFGLIRVGC 596
Score = 33.1 bits (66), Expect(4) = 3e-021
Identities = 12/24 (50%), Positives = 17/24 (70%)
Frame = +3 / +3
Query: 387 PGAVSGRFTNGYNLADLVARRLGF 458
P +GR +NG N+AD +A +LGF
Sbjct: 114 PLGATGRCSNGLNIADTIAEQLGF 185
Database: Populus_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:49 PM
Number of letters in database: 203,408,664
Number of sequences in database: 369,679
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 812,506,872
Number of Sequences: 369679
Number of extensions: 12777429
Number of successful extensions: 87821
Number of sequences better than 1.0e-020: 12
length of database: 67,802,888
effective HSP length: 54
effective length of database: 47,840,222
effective search space used: 21623780344
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)