TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 4580138.2.1
(1484 letters)
Database: Populus_nucl_with_EST.fasta
369,679 sequences; 203,408,664 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CF231481.1|CF231481 PtaC0021H9H0915 Poplar cDNA library ... 293 2e-079
gb|BU875096.1|BU875096 V002C09 Populus flower cDNA library ... 282 3e-075
gb|CN523757.1|CN523757 GQ015M04.T3_A07 GQ015 Populus tricho... 262 5e-069
gb|CA823045.1|CA823045 R19G10 two-month-old roots from clon... 221 9e-057
gb|DN491553.1|DN491553 V002C09.3pR Populus male catkins cDN... 158 1e-037
gb|CV240577.1|CV240577 WS0251.B21_M09 PT-MB-N-A-15 Populus ... 103 9e-036
gb|CV235020.1|CV235020 WS01217.B21_F24 PT-GT-FL-A-3 Populus... 103 1e-022
gb|BI127637.1|BI127637 G063P65Y Populus cambium cDNA librar... 105 7e-022
gb|CK102083.1|CK102083 G063P65.5pR Populus tension wood cDN... 105 7e-022
gb|CV227492.1|CV227492 WS0167.B21_F11 PT-DX-A-7 Populus tri... 103 3e-021
>gb|CF231481.1|CF231481 PtaC0021H9H0915 Poplar cDNA library from cambial zone Populus alba x
Populus tremula cDNA 5', mRNA sequence
Length = 771
Score = 293 bits (634), Expect(2) = 2e-079
Identities = 116/143 (81%), Positives = 131/143 (91%)
Frame = -1 / +3
Query: 1247 LMQQASIYGVAAGYCLSASLLSIINKWAIMKFPYPGALTALQYFTSVAGVLLCGQLKLIE 1068
L++Q SIYG+AAGYC+SASLLSIINKWAIMKFPYPGALTALQYFTS GVL+CG K++E
Sbjct: 213 LLKQTSIYGIAAGYCISASLLSIINKWAIMKFPYPGALTALQYFTSATGVLVCGWCKVLE 392
Query: 1067 HDGLNLRTMWKFLPAAVMFYISIFTNSELLLHADVDTFIVFRSAVPIFVAIGETLYLHQP 888
HD L L TMW+FLPAA+MFY+S+FTNSELLLHA+VDTFIVFRS VPIFVA+GETL+LHQP
Sbjct: 393 HDSLGLLTMWRFLPAAIMFYLSLFTNSELLLHANVDTFIVFRSLVPIFVAMGETLFLHQP 572
Query: 887 WPSLKTWLSLSTILGGSVIYVFT 819
WPSLKTW SLSTI GGSV+YV T
Sbjct: 573 WPSLKTWXSLSTIFGGSVLYVLT 641
Score = 177 bits (380), Expect(2) = 2e-044
Identities = 80/140 (57%), Positives = 95/140 (67%)
Frame = +1 / -3
Query: 820 VKT*ITLPPSIVESESHVLSDGHG**RYSVSPIATNIGTAERNTMKVSTSA*SRSSLFVK 999
V T TLPP +VE + HV +DGHG R VSPIAT +GT ER TMKVST A ++SSLFVK
Sbjct: 640 VSTYSTLPPKMVERDXHVFNDGHGWCRNKVSPIATKMGTNERKTMKVSTLACNKSSLFVK 461
Query: 1000 MEM*NITAAGKNFHIVLKXXXXXXXXXXXXHRRTPATLVKYCRAVRAPGYGNFMIAHLLM 1179
+ *NI AAG+N H+V + TP LVKYC AV APG GNFM+AHLLM
Sbjct: 460 SDR*NIIAAGRNRHMVKRPSESCSKTLHHPQTSTPVALVKYCNAVSAPG*GNFMMAHLLM 281
Query: 1180 IESSDADKQYPAATP*IEAC 1239
+ES DA+ QYPAA P*++ C
Sbjct: 280 MESRDAEMQYPAAMP*MDVC 221
Score = 142 bits (305), Expect = 5e-033
Identities = 58/141 (41%), Positives = 87/141 (61%)
Frame = -3 / +2
Query: 1239 ASLNLWCGCWVLFISVAALDHQQMGNHEISISWSPDSSAVFH*CCWSSPMWTAQAH*ARW 1060
A+++LW CW+L +S+ AL HQQ+G+HEI +SW + AVFH* + +W Q A +
Sbjct: 221 ANIHLWHSCWILHLSIPALHHQQVGHHEIPLSWGTNCIAVFH*RYRCTCLWVVQGFRA*F 400
Query: 1059 T*LEDNVEVLTCCCDVLHLHLHK**TPASCRCGYFHCVPLCCAYICRDRRDAVSSSAMAI 880
T D+V V TC VL + L+K * +C C Y HC+P ++C + RD V + AMAI
Sbjct: 401 TRPFDHVAVPTCSYYVLPVTLYKQ*ALIAC*C*YLHCLPFVSPHLCCNGRDLVPTPAMAI 580
Query: 879 AQDMAFTLHNTWWKCDLCFHR 817
+DM + ++ WWKC +C ++
Sbjct: 581 IEDMXVSFYHLWWKCAICANK 643
Score = 24.0 bits (46), Expect(2) = 2e-079
Identities = 8/11 (72%), Positives = 9/11 (81%)
Frame = -1 / +2
Query: 734 VVMTIGLNTWG 702
+ MTIGL TWG
Sbjct: 731 LXMTIGLKTWG 763
Score = 24.0 bits (46), Expect(2) = 2e-044
Identities = 8/9 (88%), Positives = 9/9 (100%)
Frame = +1 / -1
Query: 703 PHVFSPMVI 729
PHVF+PMVI
Sbjct: 762 PHVFNPMVI 736
>gb|BU875096.1|BU875096 V002C09 Populus flower cDNA library Populus trichocarpa cDNA 5 prime,
mRNA sequence
Length = 597
Score = 282 bits (611), Expect = 3e-075
Identities = 112/136 (82%), Positives = 127/136 (93%)
Frame = -1 / +1
Query: 1247 LMQQASIYGVAAGYCLSASLLSIINKWAIMKFPYPGALTALQYFTSVAGVLLCGQLKLIE 1068
L++ SIYGVAAGYC+SASLLSIINKWA+MKFPYPGALTALQYFTS AGVL+CG +++E
Sbjct: 190 LLKLTSIYGVAAGYCISASLLSIINKWAVMKFPYPGALTALQYFTSAAGVLVCGWFRVVE 369
Query: 1067 HDGLNLRTMWKFLPAAVMFYISIFTNSELLLHADVDTFIVFRSAVPIFVAIGETLYLHQP 888
HD L+L TMW+FLPAAVMFY+S+FTNSELLLHA+VDTFIVFRS VPIFVAIGETL+LHQP
Sbjct: 370 HDSLDLLTMWRFLPAAVMFYLSLFTNSELLLHANVDTFIVFRSLVPIFVAIGETLFLHQP 549
Query: 887 WPSLKTWLSLSTILGG 840
WPSLKTWLSL+TI GG
Sbjct: 550 WPSLKTWLSLATIFGG 597
Score = 171 bits (369), Expect = 7e-042
Identities = 77/131 (58%), Positives = 92/131 (70%)
Frame = +1 / -2
Query: 841 PPSIVESESHVLSDGHG**RYSVSPIATNIGTAERNTMKVSTSA*SRSSLFVKMEM*NIT 1020
PP +V S++HV +DGHG R VSPIAT +GT ER TM VST A S+SSL VK + *NIT
Sbjct: 596 PPKMVASDNHVFNDGHGWCRNKVSPIATKMGTNERKTMNVSTLAWSKSSLLVKSDR*NIT 417
Query: 1021 AAGKNFHIVLKXXXXXXXXXXXXHRRTPATLVKYCRAVRAPGYGNFMIAHLLMIESSDAD 1200
AAG+N HIV + TPA LVKYC+AV APG GNFM AHLL++ES DA+
Sbjct: 416 AAGRNRHIVNRSSESCSTTLNHPQTSTPAALVKYCKAVSAPG*GNFMTAHLLIMESKDAE 237
Query: 1201 KQYPAATP*IE 1233
QYPAATP*++
Sbjct: 236 MQYPAATP*MD 204
Score = 137 bits (294), Expect = 2e-031
Identities = 57/133 (42%), Positives = 80/133 (60%)
Frame = -3 / +3
Query: 1239 ASLNLWCGCWVLFISVAALDHQQMGNHEISISWSPDSSAVFH*CCWSSPMWTAQAH*ARW 1060
A+++LW CW+L +S+ AL +QQ+G+HEI + W + VFH* + +W Q A
Sbjct: 198 ANVHLWRSCWILHLSILALHYQQVGSHEIPLPWGANGFTVFH*RRRCTRLWVVQGCRA*L 377
Query: 1059 T*LEDNVEVLTCCCDVLHLHLHK**TPASCRCGYFHCVPLCCAYICRDRRDAVSSSAMAI 880
* DNV V TC C VL + L++ * C C Y HC+PL ++C +RRD V + AMAI
Sbjct: 378 A*PVDNVAVPTCGCYVLSVTLYQQ*ALTPC*CRYIHCLPLVSPHLCCNRRDLVPTPAMAI 557
Query: 879 AQDMAFTLHNTWW 841
+DM T H+ WW
Sbjct: 558 IEDMVVTCHHLWW 596
>gb|CN523757.1|CN523757 GQ015M04.T3_A07 GQ015 Populus trichocarpa x Populus deltoides cDNA
clone GQ015M04_A07 5', mRNA sequence
Length = 787
Score = 262 bits (566), Expect = 5e-069
Identities = 110/206 (53%), Positives = 147/206 (71%)
Frame = -1 / +1
Query: 1241 QQASIYGVAAGYCLSASLLSIINKWAIMKFPYPGALTALQYFTSVAGVLLCGQLKLIEHD 1062
+Q + + GY L +SLL++INK+AI KF YPG LTALQY TS GV + G+L L+ HD
Sbjct: 43 KQLATSSLVVGYALCSSLLAVINKFAITKFNYPGLLTALQYLTSALGVWVLGKLGLLYHD 222
Query: 1061 GLNLRTMWKFLPAAVMFYISIFTNSELLLHADVDTFIVFRSAVPIFVAIGETLYLHQPWP 882
+ T KFLPAA++FY++IFTN+ LL HA+VDTFIVFRS P+ VAI +TL+ QP P
Sbjct: 223 SFSYETAKKFLPAALVFYLAIFTNTNLLRHANVDTFIVFRSLTPLLVAIADTLFRKQPIP 402
Query: 881 SLKTWLSLSTILGGSVIYVFTDNQFTVTAYTWAVAYLASMSIDFVYIKHVVMTIGLNTWG 702
S T++SL ILGG+V YV TD+ FT+TAY+WA+AYL +++ + VYIKH+V IGLNTWG
Sbjct: 403 SKLTFVSLFVILGGAVGYVATDSAFTLTAYSWALAYLVTITSEMVYIKHIVSNIGLNTWG 582
Query: 701 LVLYNNXXXXXXXXXXXLVMGEFDQM 624
LV YNN ++ GE+ ++
Sbjct: 583 LVFYNNLLSLMMAPLFWILTGEYSEV 660
Score = 67.0 bits (140), Expect(3) = 2e-021
Identities = 37/74 (50%), Positives = 44/74 (59%)
Frame = +1 / -3
Query: 817 SVKT*ITLPPSIVESESHVLSDGHG**RYSVSPIATNIGTAERNTMKVSTSA*SRSSLFV 996
SV T* T PP I E++V DG G VS IAT G + NT+KVST A* + +FV
Sbjct: 467 SVAT*PTAPPKITNKETNVSLDGMGCFLNRVSAIATKRGVKDLNTIKVSTLA*RKRLVFV 288
Query: 997 KMEM*NITAAGKNF 1038
K+ * AAGKNF
Sbjct: 287 KIAR*KTRAAGKNF 246
Score = 38.2 bits (77), Expect(3) = 2e-021
Identities = 18/40 (45%), Positives = 25/40 (62%)
Frame = +1 / -3
Query: 637 SPITRSSSGNSIKASRLLYSTRPHVFSPMVITTCLMYTKS 756
SP+ SG I ++LL +TRP V SP++ T CL+ T S
Sbjct: 647 SPVRIQKSGAIINDNKLL*NTRPQVLSPILETMCLI*TIS 528
Score = 35.4 bits (71), Expect(3) = 2e-021
Identities = 15/31 (48%), Positives = 21/31 (67%)
Frame = +1 / -3
Query: 1096 RTPATLVKYCRAVRAPGYGNFMIAHLLMIES 1188
+TP V+YC AV+ PG N +IA+LL+ S
Sbjct: 188 QTPNAEVRYCNAVKRPG*LNLVIANLLITAS 96
>gb|CA823045.1|CA823045 R19G10 two-month-old roots from clone 'Beaupre' Populus trichocarpa x
Populus deltoides cDNA 5', mRNA sequence
Length = 636
Score = 221 bits (477), Expect = 9e-057
Identities = 95/174 (54%), Positives = 127/174 (72%)
Frame = -1 / +3
Query: 1241 QQASIYGVAAGYCLSASLLSIINKWAIMKFPYPGALTALQYFTSVAGVLLCGQLKLIEHD 1062
+Q + + GY L +SLL++INK+AI KF YPG LTALQY TS GV + G+L L+ HD
Sbjct: 114 KQLATSSLVVGYALCSSLLAVINKFAITKFNYPGLLTALQYLTSALGVWVLGKLGLLYHD 293
Query: 1061 GLNLRTMWKFLPAAVMFYISIFTNSELLLHADVDTFIVFRSAVPIFVAIGETLYLHQPWP 882
+ T KFLPAA++FY++IFTN+ LL HA+VDTFIVFRS P+ VAI +TL+ QP P
Sbjct: 294 SFSYETAKKFLPAALVFYLAIFTNTNLLRHANVDTFIVFRSLTPLLVAIADTLFRKQPIP 473
Query: 881 SLKTWLSLSTILGGSVIYVFTDNQFTVTAYTWAVAYLASMSIDFVYIKHVVMTI 720
S T++SL ILGG+V YV TD+ FT TAY+ A+AY+ +++ + VYIKH+V I
Sbjct: 474 SKLTFVSLCVILGGAVGYVATDSAFT*TAYSXALAYIVTITSEMVYIKHIVSXI 635
>gb|DN491553.1|DN491553 V002C09.3pR Populus male catkins cDNA library Populus trichocarpa
cDNA clone V002C09 3', mRNA sequence
Length = 748
Score = 158 bits (339), Expect = 1e-037
Identities = 68/123 (55%), Positives = 86/123 (69%)
Frame = -1 / -3
Query: 647 VMGEFDQMKVDSSKVSNWLSFDVVLPVAXXXXXXXXXXXXXXXCRRAISATGFTVLGIVN 468
+MGE ++K + S S+W SF VVLPV CRRAISATG+TVLG+VN
Sbjct: 743 IMGELKKIKHEISDESDWHSFGVVLPVGLSCSFGLAISFFGFSCRRAISATGYTVLGVVN 564
Query: 467 KLLTVVINLLIWDKHASFVGTIGLLICMSGGVLYQQSTTXXXXXXXXXXXENDEEQQKLL 288
KLLTVVINL++WDKH++F+GT+GLLICM GG++YQQST+ + DEEQQKLL
Sbjct: 563 KLLTVVINLVVWDKHSTFIGTVGLLICMLGGIMYQQSTSKPKAVPEVKAEQTDEEQQKLL 384
Query: 287 QMQ 279
+MQ
Sbjct: 383 EMQ 375
Score = 121 bits (258), Expect = 1e-026
Identities = 53/102 (51%), Positives = 67/102 (65%)
Frame = +1 / +1
Query: 340 AFGFVVDCW*STPPDMHIKSPMVPTKEACLSQISKLITTVRSLLTMPSTVNPVAEIARLQ 519
A G +VDC PP + I+ P VP CLSQ ++LITTV +L T P TV PVAEIA Q
Sbjct: 436 ALGLLVDC*YIMPPSIQIRRPTVPMNVECLSQTTRLITTVNNLFTTPRTVYPVAEIALRQ 615
Query: 520 ENPKKDIDNPNRHERATGRTTSNESQFDTLELSTFI*SNSPI 645
ENPKK++ PN ++ TG+TT E Q D+ E+S FI +SPI
Sbjct: 616 ENPKKEMAKPNEQDKPTGKTTPKECQSDSSEISCFIFLSSPI 741
>gb|CV240577.1|CV240577 WS0251.B21_M09 PT-MB-N-A-15 Populus trichocarpa cDNA clone
WS0251_M09 3', mRNA sequence
Length = 822
Score = 103 bits (219), Expect(2) = 9e-036
Identities = 45/87 (51%), Positives = 52/87 (59%)
Frame = -1 / -2
Query: 611 SKVSNWLSFDVVLPVAXXXXXXXXXXXXXXXCRRAISATGFTVLGIVNKLLTVVINLLIW 432
SK NW FD V+ R+AISAT FTV G+VNK LTVVIN+ IW
Sbjct: 638 SKTGNWFEFDAFFAVSLSCIFGFLISFFGFAARKAISATAFTVTGVVNKFLTVVINVFIW 459
Query: 431 DKHASFVGTIGLLICMSGGVLYQQSTT 351
DKHAS VG L+ ++GGVLYQQS T
Sbjct: 458 DKHASPVGLFCLVFTLAGGVLYQQSVT 378
Score = 84.0 bits (177), Expect(2) = 4e-021
Identities = 37/68 (54%), Positives = 46/68 (67%)
Frame = +1 / +1
Query: 409 PTKEACLSQISKLITTVRSLLTMPSTVNPVAEIARLQENPKKDIDNPNRHERATGRTTSN 588
PT ACLSQI+ L+TTVR+LLT P TVN VA+IA L NPKK I P H++ T + SN
Sbjct: 436 PTGLACLSQINTLMTTVRNLLTTPVTVNAVADIAFLAANPKKLIRKPKMHDKDTAKNASN 615
Query: 589 ESQFDTLE 612
+QF +
Sbjct: 616 SNQFPVFD 639
Score = 68.4 bits (143), Expect(2) = 9e-036
Identities = 25/56 (44%), Positives = 37/56 (66%)
Frame = -1 / -2
Query: 791 TWAVAYLASMSIDFVYIKHVVMTIGLNTWGLVLYNNXXXXXXXXXXXLVMGEFDQM 624
+WA+AYL +++ + VYIKH+V IGLNTWGLV YNN ++ GE+ ++
Sbjct: 821 SWALAYLVTITSEMVYIKHIVSNIGLNTWGLVFYNNLLSLMMAPLFWILTGEYSEV 654
Score = 38.2 bits (77), Expect(2) = 4e-021
Identities = 18/40 (45%), Positives = 25/40 (62%)
Frame = +1 / +1
Query: 637 SPITRSSSGNSIKASRLLYSTRPHVFSPMVITTCLMYTKS 756
SP+ SG I ++LL +TRP V SP++ T CL+ T S
Sbjct: 667 SPVRIQKSGAIINDNKLL*NTRPQVLSPILETMCLI*TIS 786
>gb|CV235020.1|CV235020 WS01217.B21_F24 PT-GT-FL-A-3 Populus trichocarpa cDNA clone
WS01217_F24 3', mRNA sequence
Length = 694
Score = 103 bits (219), Expect(2) = 1e-022
Identities = 45/87 (51%), Positives = 52/87 (59%)
Frame = -1 / -3
Query: 611 SKVSNWLSFDVVLPVAXXXXXXXXXXXXXXXCRRAISATGFTVLGIVNKLLTVVINLLIW 432
SK NW FD V+ R+AISAT FTV G+VNK LTVVIN+ IW
Sbjct: 575 SKTGNWFEFDAFFAVSLSCIFGFLISFFGFAARKAISATAFTVTGVVNKFLTVVINVFIW 396
Query: 431 DKHASFVGTIGLLICMSGGVLYQQSTT 351
DKHAS VG L+ ++GGVLYQQS T
Sbjct: 395 DKHASPVGLFCLVFTLAGGVLYQQSVT 315
Score = 24.0 bits (46), Expect(2) = 1e-022
Identities = 8/8 (100%), Positives = 8/8 (100%)
Frame = -1 / -1
Query: 722 IGLNTWGL 699
IGLNTWGL
Sbjct: 688 IGLNTWGL 665
>gb|BI127637.1|BI127637 G063P65Y Populus cambium cDNA library Populus tremula x Populus
tremuloides cDNA, mRNA sequence
Length = 297
Score = 105 bits (224), Expect = 7e-022
Identities = 44/70 (62%), Positives = 54/70 (77%)
Frame = -1 / +3
Query: 470 NKLLTVVINLLIWDKHASFVGTIGLLICMSGGVLYQQSTTXXXXXXXXXXXENDEEQQKL 291
NKLLTVVINL+IWDKH++FVGT+GLLICM GG++YQQST+ + DEEQQKL
Sbjct: 3 NKLLTVVINLVIWDKHSTFVGTVGLLICMLGGIMYQQSTSKPKAVPEVKAEQTDEEQQKL 182
Query: 290 LQMQGVQESS 261
L+MQ ES+
Sbjct: 183 LEMQSNIESN 212
>gb|CK102083.1|CK102083 G063P65.5pR Populus tension wood cDNA library Populus tremula x
Populus tremuloides cDNA clone G063P65 5', mRNA sequence
Length = 437
Score = 105 bits (224), Expect = 7e-022
Identities = 44/70 (62%), Positives = 54/70 (77%)
Frame = -1 / +3
Query: 470 NKLLTVVINLLIWDKHASFVGTIGLLICMSGGVLYQQSTTXXXXXXXXXXXENDEEQQKL 291
NKLLTVVINL+IWDKH++FVGT+GLLICM GG++YQQST+ + DEEQQKL
Sbjct: 3 NKLLTVVINLVIWDKHSTFVGTVGLLICMLGGIMYQQSTSKPKAVPEVKAEQTDEEQQKL 182
Query: 290 LQMQGVQESS 261
L+MQ ES+
Sbjct: 183 LEMQSNIESN 212
>gb|CV227492.1|CV227492 WS0167.B21_F11 PT-DX-A-7 Populus trichocarpa cDNA clone WS0167_F11
3', mRNA sequence
Length = 572
Score = 103 bits (219), Expect = 3e-021
Identities = 45/87 (51%), Positives = 52/87 (59%)
Frame = -1 / +2
Query: 611 SKVSNWLSFDVVLPVAXXXXXXXXXXXXXXXCRRAISATGFTVLGIVNKLLTVVINLLIW 432
SK NW FD V+ R+AISAT FTV G+VNK LTVVIN+ IW
Sbjct: 32 SKTGNWFEFDAFFAVSLSCIFGFLISFFGFAARKAISATAFTVTGVVNKFLTVVINVFIW 211
Query: 431 DKHASFVGTIGLLICMSGGVLYQQSTT 351
DKHAS VG L+ ++GGVLYQQS T
Sbjct: 212 DKHASPVGLFCLVFTLAGGVLYQQSVT 292
Database: Populus_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:49 PM
Number of letters in database: 203,408,664
Number of sequences in database: 369,679
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 771,602,102
Number of Sequences: 369679
Number of extensions: 11787518
Number of successful extensions: 59014
Number of sequences better than 1.0e-020: 13
length of database: 67,802,888
effective HSP length: 54
effective length of database: 47,840,222
effective search space used: 21049697680
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)