TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3754578.2.1
(725 letters)
Database: Populus_nucl_with_EST.fasta
369,679 sequences; 203,408,664 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CK088334.1|CK088334 A048P19.3pR Hybrid aspen plasmid lib... 156 1e-037
gb|CV232041.1|CV232041 WS0196.B21_F15 PT-DX-N-A-10 Populus ... 156 1e-037
gb|CV277661.1|CV277661 WS0144.B21_D09 PTxD-IL-A-5 Populus t... 138 5e-032
gb|BI071270.1|BI071270 C054P51U Populus strain T89 leaves P... 117 6e-026
gb|CV237411.1|CV237411 WS01227.B21.1_N22 PT-GT-FL-A-3 Popul... 117 6e-026
gb|AJ774176.1|AJ774176 AJ774176 Populus euphratica shoot 3-... 113 1e-024
>gb|CK088334.1|CK088334 A048P19.3pR Hybrid aspen plasmid library Populus tremula x Populus
tremuloides cDNA clone A048P19 3', mRNA sequence
Length = 803
Score = 156 bits (336), Expect = 1e-037
Identities = 54/65 (83%), Positives = 62/65 (95%)
Frame = -3 / -1
Query: 717 EDVSMGLWVEKFNSTNPVQYSHSWKFCQYGCLENYYTAHYQSPRQMLCLWDKLVRGQASC 538
EDVSMG+WVE+FNS+ PVQYSH+WKFCQYGC+ENYYTAHYQSPRQM+CLWDKL RG+A C
Sbjct: 476 EDVSMGMWVEQFNSSTPVQYSHNWKFCQYGCMENYYTAHYQSPRQMICLWDKLARGRAQC 297
Query: 537 CNYR* 523
CN+R*
Sbjct: 296 CNFR* 282
Score = 101 bits (216), Expect = 4e-021
Identities = 46/69 (66%), Positives = 51/69 (73%)
Frame = +2 / +1
Query: 518 LCYL*LQQDA*PRTNLSHKHSICLGD**WAV**FSRQPYWQNFQLWEYWTGFVELNFSTH 697
+C+L LQ A P NLSHK ICLGD * AV**FS PY QNFQLWEYWTG LN STH
Sbjct: 277 ICHLKLQH*ARPLANLSHKQIICLGDW*CAV**FSMHPY*QNFQLWEYWTGVELLNCSTH 456
Query: 698 RPMLTSSLV 724
P+LTSS++
Sbjct: 457 IPILTSSIL 483
>gb|CV232041.1|CV232041 WS0196.B21_F15 PT-DX-N-A-10 Populus trichocarpa cDNA clone
WS0196_F15 3', mRNA sequence
Length = 663
Score = 156 bits (336), Expect = 1e-037
Identities = 54/65 (83%), Positives = 62/65 (95%)
Frame = -3 / -2
Query: 717 EDVSMGLWVEKFNSTNPVQYSHSWKFCQYGCLENYYTAHYQSPRQMLCLWDKLVRGQASC 538
EDVSMG+WVE+FNS+ PVQYSH+WKFCQYGC+ENYYTAHYQSPRQM+CLWDKL RG+A C
Sbjct: 530 EDVSMGMWVEQFNSSTPVQYSHNWKFCQYGCMENYYTAHYQSPRQMICLWDKLARGRAQC 351
Query: 537 CNYR* 523
CN+R*
Sbjct: 350 CNFR* 336
>gb|CV277661.1|CV277661 WS0144.B21_D09 PTxD-IL-A-5 Populus trichocarpa x Populus deltoides
cDNA clone WS0144_D09 3', mRNA sequence
Length = 877
Score = 138 bits (295), Expect = 5e-032
Identities = 48/65 (73%), Positives = 59/65 (90%)
Frame = -3 / -3
Query: 717 EDVSMGLWVEKFNSTNPVQYSHSWKFCQYGCLENYYTAHYQSPRQMLCLWDKLVRGQASC 538
EDVSMG+WVEKFN++ PV+Y HS KFCQ+GC+E+YYTAHYQSPRQM+CLWDKL +G++ C
Sbjct: 572 EDVSMGMWVEKFNNSKPVEYIHSLKFCQFGCIEDYYTAHYQSPRQMICLWDKLNQGKSQC 393
Query: 537 CNYR* 523
CN R*
Sbjct: 392 CNMR* 378
>gb|BI071270.1|BI071270 C054P51U Populus strain T89 leaves Populus tremula x Populus
tremuloides cDNA, mRNA sequence
Length = 340
Score = 117 bits (251), Expect = 6e-026
Identities = 40/53 (75%), Positives = 49/53 (92%)
Frame = -3 / +2
Query: 717 EDVSMGLWVEKFNSTNPVQYSHSWKFCQYGCLENYYTAHYQSPRQMLCLWDKL 559
EDVSMG+WVE+FNS+ PV+Y HS KFCQ+GC+E YYTAHYQSP+QM+CLW+KL
Sbjct: 110 EDVSMGMWVEQFNSSRPVEYVHSLKFCQFGCIEGYYTAHYQSPKQMICLWEKL 268
>gb|CV237411.1|CV237411 WS01227.B21.1_N22 PT-GT-FL-A-3 Populus trichocarpa cDNA clone
WS01227_N22 3', mRNA sequence
Length = 867
Score = 117 bits (251), Expect = 6e-026
Identities = 40/53 (75%), Positives = 49/53 (92%)
Frame = -3 / -3
Query: 717 EDVSMGLWVEKFNSTNPVQYSHSWKFCQYGCLENYYTAHYQSPRQMLCLWDKL 559
EDVSMG+WVE+FNS+ PV+Y HS KFCQ+GC+E YYTAHYQSP+QM+CLW+KL
Sbjct: 565 EDVSMGMWVEQFNSSRPVEYVHSLKFCQFGCIEGYYTAHYQSPKQMICLWEKL 407
>gb|AJ774176.1|AJ774176 AJ774176 Populus euphratica shoot 3-6 months Populus euphratica
cDNA clone P0000300013F04F1, mRNA sequence
Length = 366
Score = 113 bits (242), Expect = 1e-024
Identities = 40/61 (65%), Positives = 50/61 (81%)
Frame = -3 / +1
Query: 717 EDVSMGLWVEKFNSTNPVQYSHSWKFCQYGCLENYYTAHYQSPRQMLCLWDKLVRGQASC 538
EDVSMG+WVE+FNS+ PV+Y HS KFCQ GC+E YYT HYQ+PRQM+CLW+KL + +
Sbjct: 112 EDVSMGMWVEQFNSSIPVEYVHSLKFCQLGCIEGYYTGHYQAPRQMICLWEKLQKQGRAQ 291
Query: 537 C 535
C
Sbjct: 292 C 294
Database: Populus_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:49 PM
Number of letters in database: 203,408,664
Number of sequences in database: 369,679
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 418,677,733
Number of Sequences: 369679
Number of extensions: 6557603
Number of successful extensions: 53708
Number of sequences better than 1.0e-020: 6
length of database: 67,802,888
effective HSP length: 52
effective length of database: 48,579,580
effective search space used: 9181540620
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)