TBLASTX 2.2.10 [Oct-19-2004]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 3712967.2.1
         (571 letters)

Database: Populus_nucl_with_EST.fasta 
           369,679 sequences; 203,408,664 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|DN493683.1|DN493683  G105P21.5pR Populus tension wood cDN...   118   3e-026
gb|CV273245.1|CV273245  WS01710.B21_C19 PTxD-NR-A-8 Populus ...    88   2e-021
gb|CK089666.1|CK089666  C025P31.3pR Populus strain T89 leave...    89   1e-020
gb|CA824648.1|CA824648  R46B01 two-month-old roots from clon...    91   1e-020

>gb|DN493683.1|DN493683 G105P21.5pR Populus tension wood cDNA library Populus tremula x
           Populus tremuloides cDNA clone G105P21 5', mRNA sequence
          Length = 677

 Score =  118 bits (252), Expect = 3e-026
 Identities = 43/95 (45%), Positives = 60/95 (63%)
 Frame = +2 / +1

                                                                       
Query: 68  VNTYLGRPWKEFARTVVMESVIDGFVHRQGYMPWEGKDNLGTAFFGEFRNGGDGANVTGR 247
           V TYLGRPWK+++RTV M+S +D  ++  G+  W G   L T+++ E+ N G G+N T R
Sbjct: 277 VQTYLGRPWKQYSRTVYMQSFMDSLINPAGWQIWSGDFALNTSYYAEYNNSGPGSNTTNR 456

                                              
Query: 248 KEMQGFHVMGKDRALQFTVGHFLHGADWIPETGTP 352
               GFHV+    A+ FTV  FL G DW+P+T  P
Sbjct: 457 VTWPGFHVINATDAVNFTVSSFLMGNDWLPQTAVP 561



>gb|CV273245.1|CV273245 WS01710.B21_C19 PTxD-NR-A-8 Populus trichocarpa x Populus deltoides
           cDNA clone WS01710_C19 3', mRNA sequence
          Length = 618

 Score = 87.7 bits (185), Expect(2) = 2e-021
 Identities = 30/67 (44%), Positives = 47/67 (70%)
 Frame = +2 / -3

                                                                       
Query: 74  TYLGRPWKEFARTVVMESVIDGFVHRQGYMPWEGKDNLGTAFFGEFRNGGDGANVTGRKE 253
           TYLGRPWKE++RTV +++ +D  V   G++ W+G   L T ++GE+RN G GA+  GR +
Sbjct: 478 TYLGRPWKEYSRTVFLQTYLDSLVDPAGWLEWDGNFALNTLYYGEYRNSGPGASTRGRVK 299

                  
Query: 254 MQGFHVM 274
            +G+ V+
Sbjct: 298 WRGYRVI 278




 Score = 34.1 bits (68), Expect(2) = 2e-021
 Identities = 11/28 (39%), Positives = 16/28 (57%)
 Frame = +2 / -3

                                       
Query: 269 VMGKDRALQFTVGHFLHGADWIPETGTP 352
           +     A +FTV +F+ G  W+P TG P
Sbjct: 280 ITSSTEASRFTVANFIAGRSWLPATGVP 197



>gb|CK089666.1|CK089666 C025P31.3pR Populus strain T89 leaves Populus tremula x Populus
           tremuloides cDNA clone C025P31 3', mRNA sequence
          Length = 535

 Score = 89.0 bits (188), Expect(2) = 1e-020
 Identities = 30/65 (46%), Positives = 46/65 (70%)
 Frame = +2 / -3

                                                                       
Query: 74  TYLGRPWKEFARTVVMESVIDGFVHRQGYMPWEGKDNLGTAFFGEFRNGGDGANVTGRKE 253
           TYLGRPWKE++RTVVM+S++DG +   G+ PW G  +  + ++GE+ N G G++ +GR  
Sbjct: 494 TYLGRPWKEYSRTVVMQSLLDGSIDPAGWFPWAGGSSPKSIYYGEYSNSGPGSSTSGRVN 315

                
Query: 254 MQGFH 268
             G+H
Sbjct: 314 WPGYH 300




 Score = 30.4 bits (60), Expect(2) = 1e-020
 Identities = 11/22 (50%), Positives = 14/22 (63%)
 Frame = +2 / -3

                                 
Query: 281 DRALQFTVGHFLHGADWIPETG 346
           + A  FTVG F+ G  W+P TG
Sbjct: 284 EEAQGFTVGSFISGNVWLPPTG 219



>gb|CA824648.1|CA824648 R46B01 two-month-old roots from clone 'Beaupre' Populus trichocarpa
           x Populus deltoides cDNA 5', mRNA sequence
          Length = 494

 Score = 90.9 bits (192), Expect(2) = 1e-020
 Identities = 29/65 (44%), Positives = 46/65 (70%)
 Frame = +2 / +2

                                                                       
Query: 74  TYLGRPWKEFARTVVMESVIDGFVHRQGYMPWEGKDNLGTAFFGEFRNGGDGANVTGRKE 253
           TYLGRPWK+++RTV+M+S +DG +H  G+ PW G  +  + ++GE+ N G G++ +GR  
Sbjct: 92  TYLGRPWKQYSRTVIMQSSLDGSIHPAGWFPWAGGSSPSSIYYGEYSNSGPGSSTSGRVN 271

                
Query: 254 MQGFH 268
             G+H
Sbjct: 272 WPGYH 286




 Score = 28.6 bits (56), Expect(2) = 1e-020
 Identities = 10/20 (50%), Positives = 13/20 (65%)
 Frame = +2 / +2

                               
Query: 287 ALQFTVGHFLHGADWIPETG 346
           A +FTVG F+ G  W+P  G
Sbjct: 308 AQKFTVGSFISGNVWLPPAG 367



  Database: Populus_nucl_with_EST.fasta
    Posted date:  Feb 7, 2006  2:49 PM
  Number of letters in database: 203,408,664
  Number of sequences in database:  369,679
  
Lambda     K      H
   0.318    0.134    0.401 


Matrix: BLOSUM62
Number of Hits to DB: 206,250,604
Number of Sequences: 369679
Number of extensions: 2539532
Number of successful extensions: 47694
Number of sequences better than 1.0e-020: 5
length of database: 67,802,888
effective HSP length: 51
effective length of database: 48,949,259
effective search space used: 6754997742
frameshift window, decay const: 40,  0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)