TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3670899.2.1
(555 letters)
Database: Populus_nucl_with_EST.fasta
369,679 sequences; 203,408,664 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BP933583.1|BP933583 BP933583 full-length enriched poplar... 111 6e-041
gb|CV282822.1|CV282822 WS0185.B21_L03 PTxD-IL-N-A-9 Populus... 116 5e-036
gb|BP932582.1|BP932582 BP932582 full-length enriched poplar... 87 1e-033
gb|DT520820.1|DT520820 WS02448.B21_K02 PTxD-ICC-N-A-14 Popu... 108 2e-031
>gb|BP933583.1|BP933583 BP933583 full-length enriched poplar cDNA library Populus nigra
cDNA clone PnFL1-053_K10.r 3', mRNA sequence
Length = 617
Score = 111 bits (236), Expect(2) = 6e-041
Identities = 44/75 (58%), Positives = 54/75 (72%)
Frame = -3 / -1
Query: 538 MYYESPTKSGEIVAVFQPSHEEQQQYTSNDHNQKALAEIYLLSYCDKITMSAWSTFGYVA 359
MY+E PT +GEIV V+QPSHEE QQ HN+KA AE+YLLS D + SAWSTFGYVA
Sbjct: 605 MYWEHPTMTGEIVGVYQPSHEEFQQTEKQMHNRKAWAEMYLLSLTDVLVTSAWSTFGYVA 426
Query: 358 YSFAGVKPWILIRPD 314
G++PWIL + +
Sbjct: 425 QGLGGLRPWILYKTE 381
Score = 76.2 bits (160), Expect(2) = 6e-041
Identities = 26/56 (46%), Positives = 36/56 (64%)
Frame = -3 / -1
Query: 310 NRETSDVACVRSTSVEPCLHSPPILGCRAKTDVDVAAVKPYVRHCEDVGFGLKLFD 143
N D C R+ S+EPC H+PP C+AK +D + P+VRHCED+ +GLK+ D
Sbjct: 380 NDTAPDPPCRRAMSMEPCFHAPPFYDCKAKKGIDTGTLVPHVRHCEDISWGLKVVD 213
>gb|CV282822.1|CV282822 WS0185.B21_L03 PTxD-IL-N-A-9 Populus trichocarpa x Populus
deltoides cDNA clone WS0185_L03 3', mRNA sequence
Length = 874
Score = 116 bits (248), Expect(2) = 5e-036
Identities = 44/74 (59%), Positives = 54/74 (72%)
Frame = -3 / -2
Query: 538 MYYESPTKSGEIVAVFQPSHEEQQQYTSNDHNQKALAEIYLLSYCDKITMSAWSTFGYVA 359
MY+ PT +GE+++V+QPSHEE Q + N HN KA A+IYLLS CD + S WSTFGYVA
Sbjct: 510 MYWTRPTVTGEVISVYQPSHEEYQHFGDNTHNMKAWADIYLLSLCDVLVTSTWSTFGYVA 331
Query: 358 YSFAGVKPWILIRP 317
AG KPWIL +P
Sbjct: 330 AGLAGWKPWILRKP 289
Score = 86.8 bits (183), Expect(2) = 1e-021
Identities = 43/74 (58%), Positives = 46/74 (62%)
Frame = +3 / +2
Query: 318 GRIRIHGFTPAKLYAT*PKVDQALIVILSQ*LSR*ISASAFWLWSLDVYCCCSSWLGWNT 497
G +IHGF PAK AT*PKVDQ L+ S LSR ISA AF L L C SSWLGW T
Sbjct: 290 GLRKIHGFQPAKPAAT*PKVDQVLVTSTSHKLSRYISAHAFMLCVLSPKCWYSSWLGWYT 469
Query: 498 ATISPLLVGLS*YM 539
SP+ VGL YM
Sbjct: 470 LMTSPVTVGLVQYM 511
Score = 54.2 bits (112), Expect(2) = 5e-036
Identities = 21/47 (44%), Positives = 28/47 (59%)
Frame = -3 / -2
Query: 289 ACVRSTSVEPCLHSPPILGCRAKTDVDVAAVKPYVRHCEDVGFGLKL 149
AC R+ S+EPC H P C+ + +V+ A + P HCED GLKL
Sbjct: 273 ACQRAISMEPCCHFPLSYDCKTRIEVNAATLVPNTMHCEDRKNGLKL 133
Score = 35.4 bits (71), Expect(2) = 1e-021
Identities = 17/44 (38%), Positives = 23/44 (52%)
Frame = +3 / +2
Query: 150 NLRPKPTSSQCRTYGLTAATSTSVLALHPRIGGECRHGSTDVDR 281
+ P SSQC G A TS+L L + G+ +HGS D+ R
Sbjct: 134 SFNPFLRSSQCIVLGTRVAALTSILVLQS*LKGK*QHGSIDMAR 265
>gb|BP932582.1|BP932582 BP932582 full-length enriched poplar cDNA library Populus nigra
cDNA clone PnFL1-037_M07.r 3', mRNA sequence
Length = 563
Score = 86.8 bits (183), Expect(2) = 1e-033
Identities = 34/61 (55%), Positives = 43/61 (70%)
Frame = -3 / -1
Query: 496 VFQPSHEEQQQYTSNDHNQKALAEIYLLSYCDKITMSAWSTFGYVAYSFAGVKPWILIRP 317
V+QPSHEE QQ HN++A AE+YLLS D + SAWSTFGYVA G++PWIL +
Sbjct: 560 VYQPSHEEFQQTEKQMHNRQAWAEMYLLSLTDVLVTSAWSTFGYVAQGLGGLRPWILYKT 381
Query: 316 D 314
+
Sbjct: 380 E 378
Score = 76.2 bits (160), Expect(2) = 1e-033
Identities = 26/56 (46%), Positives = 36/56 (64%)
Frame = -3 / -1
Query: 310 NRETSDVACVRSTSVEPCLHSPPILGCRAKTDVDVAAVKPYVRHCEDVGFGLKLFD 143
N D C R+ S+EPC H+PP C+AK +D + P+VRHCED+ +GLK+ D
Sbjct: 377 NDTAPDPPCRRAMSMEPCFHAPPFYDCKAKKGIDTGTLVPHVRHCEDISWGLKVVD 210
>gb|DT520820.1|DT520820 WS02448.B21_K02 PTxD-ICC-N-A-14 Populus trichocarpa x Populus
deltoides cDNA clone WS02448_K02 3', mRNA sequence
Length = 866
Score = 108 bits (230), Expect(3) = 2e-031
Identities = 42/80 (52%), Positives = 55/80 (68%)
Frame = -3 / -3
Query: 538 MYYESPTKSGEIVAVFQPSHEEQQQYTSNDHNQKALAEIYLLSYCDKITMSAWSTFGYVA 359
MY+ PT +G+++ V+QPSHEE Q + +N HN KA AEI +LS D + S+WSTFGYVA
Sbjct: 507 MYWMKPTVNGDVIGVYQPSHEEVQHFGNNIHNMKAWAEISILSLSDVLVTSSWSTFGYVA 328
Query: 358 YSFAGVKPWILIRPDWNRET 299
G+KPWIL P N+ T
Sbjct: 327 QGLGGLKPWILYVPAGNQPT 268
Score = 33.6 bits (67), Expect(3) = 2e-031
Identities = 11/25 (44%), Positives = 18/25 (72%)
Frame = -3 / -3
Query: 214 VDVAAVKPYVRHCEDVGFGLKLFDS 140
+D ++ P+VR CED G+KLF++
Sbjct: 180 LDTGSLVPHVRQCEDASKGIKLFNA 106
Score = 31.3 bits (62), Expect(3) = 2e-031
Identities = 10/21 (47%), Positives = 13/21 (61%)
Frame = -3 / -3
Query: 304 ETSDVACVRSTSVEPCLHSPP 242
+ +D C R S+EPC H PP
Sbjct: 276 QPTDQPCPRGMSMEPCFHFPP 214
Database: Populus_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:49 PM
Number of letters in database: 203,408,664
Number of sequences in database: 369,679
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 228,751,891
Number of Sequences: 369679
Number of extensions: 2723147
Number of successful extensions: 55681
Number of sequences better than 1.0e-020: 4
length of database: 67,802,888
effective HSP length: 51
effective length of database: 48,949,259
effective search space used: 6510251447
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)