TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3336981.2.1
(510 letters)
Database: Populus_nucl_with_EST.fasta
369,679 sequences; 203,408,664 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CV242314.1|CV242314 WS02514.B21.1_L16 PT-MB-N-A-15 Popul... 189 8e-055
gb|CA923792.1|CA923792 MTU7CL.P15.A06 Aspen leaf cDNA Libra... 176 4e-051
gb|CN517495.1|CN517495 GQ0092.B3_O17 GQ009 Populus trichoca... 176 1e-043
gb|DN503442.1|DN503442 Y016A06.5pR Populus infected leaf su... 167 5e-041
gb|CV264688.1|CV264688 WS02025.B21_D03 PTxN-IB-N-A-11 Popul... 146 1e-034
gb|CV264913.1|CV264913 WS02025.B21_N09 PTxN-IB-N-A-11 Popul... 132 1e-030
gb|DT524332.1|DT524332 WS02042.C21.1_F13 PTxN-IB-N-A-11 Pop... 68 7e-025
gb|CX176614.1|CX176614 C10_69-38_06.ab1 leaf inoculated wit... 62 3e-024
gb|CX171013.1|CX171013 E12_69-102_10.ab1 leaf inoculated wi... 59 2e-023
gb|DT493142.1|DT493142 WS02553.C21_I10 PT-MB-N-A-15 Populus... 104 5e-022
>gb|CV242314.1|CV242314 WS02514.B21.1_L16 PT-MB-N-A-15 Populus trichocarpa cDNA clone
WS02514_L16 3', mRNA sequence
Length = 908
Score = 189 bits (407), Expect(2) = 8e-055
Identities = 77/143 (53%), Positives = 99/143 (69%)
Frame = +1 / -3
Query: 82 STYSSIQSRCEVIMYTIALLTFMSIGGFPSFVEDVKVFRKERLSGHYGVSEFVISNTISA 261
S SIQ R ++M+ + LTFM+IGGFPSFVED+KVF +ERL+GHYG + FV NT SA
Sbjct: 702 SDNDSIQDRGSLLMFIASFLTFMTIGGFPSFVEDMKVFERERLNGHYGATAFVFGNTFSA 523
Query: 262 TPYLAGVSLLPGAMLYYLTGLSKGADHFVYFXXXXXXXXXXXXXXXXXIAAVVPDFLMGI 441
PYL +S++PGA+ YYL GL KG + F+YF +A++VP+FLMGI
Sbjct: 522 FPYLLLISVIPGAITYYLPGLHKGYEQFIYFVLVLFACMMLVESLMMTVASMVPNFLMGI 343
Query: 442 IVGAGVQGVMMLNGGFFRLPNEL 510
I GAG+Q M+L GGFFRLPN+L
Sbjct: 342 ITGAGIQAFMVLGGGFFRLPNDL 274
Score = 44.1 bits (90), Expect(2) = 8e-055
Identities = 14/26 (53%), Positives = 21/26 (80%)
Frame = +1 / -3
Query: 1 VGYYWMRLAIYMGIGVCLGTIFYQLG 78
+GYYW+R+AIY+ + + L T+FY LG
Sbjct: 780 LGYYWLRVAIYVALALGLATLFYNLG 703
>gb|CA923792.1|CA923792 MTU7CL.P15.A06 Aspen leaf cDNA Library Populus tremuloides cDNA,
mRNA sequence
Length = 704
Score = 176 bits (379), Expect(2) = 4e-051
Identities = 69/141 (48%), Positives = 98/141 (69%)
Frame = +1 / -1
Query: 88 YSSIQSRCEVIMYTIALLTFMSIGGFPSFVEDVKVFRKERLSGHYGVSEFVISNTISATP 267
Y+SI +R + +TFMSIGGFPSFVE++KVF++ERL+GHYGV+ FVISNTISA P
Sbjct: 452 YNSILARGSCASFVFGFVTFMSIGGFPSFVEEMKVFQRERLNGHYGVAAFVISNTISAMP 273
Query: 268 YLAGVSLLPGAMLYYLTGLSKGADHFVYFXXXXXXXXXXXXXXXXXIAAVVPDFLMGIIV 447
+L ++ + G + Y++ L G +H+V+F IA++VP+FLMGII+
Sbjct: 272 FLIMITFISGTVCYFMVRLHPGFEHYVFFVLCLYASVAVVESLMMTIASIVPNFLMGIII 93
Query: 448 GAGVQGVMMLNGGFFRLPNEL 510
GAG+QG+ ML G+FRLPN++
Sbjct: 92 GAGIQGIFMLVSGYFRLPNDI 30
Score = 44.6 bits (91), Expect(2) = 4e-051
Identities = 14/25 (56%), Positives = 20/25 (80%)
Frame = +1 / -1
Query: 4 GYYWMRLAIYMGIGVCLGTIFYQLG 78
GYYW+RL IY+ + VC+GTI+ +G
Sbjct: 533 GYYWLRLVIYIVVTVCIGTIYLNVG 459
>gb|CN517495.1|CN517495 GQ0092.B3_O17 GQ009 Populus trichocarpa x Populus deltoides cDNA
clone GQ0092_O17 5', mRNA sequence
Length = 552
Score = 176 bits (378), Expect = 1e-043
Identities = 68/122 (55%), Positives = 90/122 (73%)
Frame = +1 / +1
Query: 145 FMSIGGFPSFVEDVKVFRKERLSGHYGVSEFVISNTISATPYLAGVSLLPGAMLYYLTGL 324
FM+IGGFPSFVE++KVF +ERL+GHYG + F I NT SA P+L +SL+PGA+ YY +GL
Sbjct: 1 FMAIGGFPSFVEEMKVFERERLNGHYGATSFAIGNTFSAIPFLLLISLIPGAIAYYPSGL 180
Query: 325 SKGADHFVYFXXXXXXXXXXXXXXXXXIAAVVPDFLMGIIVGAGVQGVMMLNGGFFRLPN 504
KG +HF+YF +A++VP+FLMGII GAG+QG+M+L GGFFRLP+
Sbjct: 181 HKGYEHFLYFASTLFACLIMVESMMMAVASIVPNFLMGIITGAGIQGLMVLGGGFFRLPD 360
Query: 505 EL 510
+L
Sbjct: 361 DL 366
>gb|DN503442.1|DN503442 Y016A06.5pR Populus infected leaf substracted cDNA library Populus
tremula cDNA clone Y016A06 5', mRNA sequence
Length = 832
Score = 167 bits (359), Expect = 5e-041
Identities = 65/124 (52%), Positives = 90/124 (72%)
Frame = +1 / +1
Query: 139 LTFMSIGGFPSFVEDVKVFRKERLSGHYGVSEFVISNTISATPYLAGVSLLPGAMLYYLT 318
+TFMSIGGFPSFVE++KVF++ERL+GHYGV FVISNTISA P+L ++ + G + Y++
Sbjct: 7 VTFMSIGGFPSFVEEMKVFQRERLNGHYGVVAFVISNTISAMPFLIMITFISGTVCYFMV 186
Query: 319 GLSKGADHFVYFXXXXXXXXXXXXXXXXXIAAVVPDFLMGIIVGAGVQGVMMLNGGFFRL 498
L G +H+V+F IA++VP+FLMGII+GAG+QG+ ML G+FRL
Sbjct: 187 RLHPGFEHYVFFVLCLYASVTVVESLMMTIASIVPNFLMGIIIGAGIQGIFMLVSGYFRL 366
Query: 499 PNEL 510
PN++
Sbjct: 367 PNDI 378
>gb|CV264688.1|CV264688 WS02025.B21_D03 PTxN-IB-N-A-11 Populus trichocarpa x Populus nigra
cDNA clone WS02025_D03 3', mRNA sequence
Length = 838
Score = 146 bits (313), Expect = 1e-034
Identities = 56/110 (50%), Positives = 77/110 (70%)
Frame = +1 / -3
Query: 181 DVKVFRKERLSGHYGVSEFVISNTISATPYLAGVSLLPGAMLYYLTGLSKGADHFVYFXX 360
++KVF +ERL+GHYG + F I NT SA P+L +SL+PGA+ YY +GL KG +HF+YF
Sbjct: 836 EMKVFERERLNGHYGATSFAIGNTFSAIPFLLLISLIPGAIAYYPSGLHKGYEHFLYFAS 657
Query: 361 XXXXXXXXXXXXXXXIAAVVPDFLMGIIVGAGVQGVMMLNGGFFRLPNEL 510
+A++VP++LMGII GAG+QG+M L GGFFRLP++L
Sbjct: 656 TLFACLLMVESMMMAVASIVPNYLMGIITGAGIQGLMALGGGFFRLPDDL 507
>gb|CV264913.1|CV264913 WS02025.B21_N09 PTxN-IB-N-A-11 Populus trichocarpa x Populus nigra
cDNA clone WS02025_N09 3', mRNA sequence
Length = 810
Score = 132 bits (283), Expect = 1e-030
Identities = 51/101 (50%), Positives = 69/101 (68%)
Frame = +1 / -2
Query: 208 LSGHYGVSEFVISNTISATPYLAGVSLLPGAMLYYLTGLSKGADHFVYFXXXXXXXXXXX 387
L+GHYG + F I NT SA P+L +SL+PGA+ YY +GL KG +HF+YF
Sbjct: 809 LNGHYGATSFAIGNTFSAIPFLLLISLIPGAIAYYPSGLHKGYEHFLYFASTLFACLLMV 630
Query: 388 XXXXXXIAAVVPDFLMGIIVGAGVQGVMMLNGGFFRLPNEL 510
+A++VP++LMGII GAG+QG+M L GGFFRLP++L
Sbjct: 629 ESMMMAVASIVPNYLMGIITGAGIQGLMALGGGFFRLPDDL 507
>gb|DT524332.1|DT524332 WS02042.C21.1_F13 PTxN-IB-N-A-11 Populus trichocarpa x Populus
nigra cDNA clone WS02042_F13 3', mRNA sequence
Length = 866
Score = 68.0 bits (142), Expect(2) = 7e-025
Identities = 26/66 (39%), Positives = 38/66 (57%)
Frame = +1 / -1
Query: 313 LTGLSKGADHFVYFXXXXXXXXXXXXXXXXXIAAVVPDFLMGIIVGAGVQGVMMLNGGFF 492
L KG +HF+Y +A++VP+++MGII GAG+QG+M L GGFF
Sbjct: 677 LPAFYKGYEHFLYLASSLLASLLMVESMMLAVASIVPNYVMGIITGAGIQGLMALGGGFF 498
Query: 493 RLPNEL 510
P++L
Sbjct: 497 WSPDDL 480
Score = 65.2 bits (136), Expect(2) = 7e-025
Identities = 24/46 (52%), Positives = 34/46 (73%)
Frame = +1 / -3
Query: 187 KVFRKERLSGHYGVSEFVISNTISATPYLAGVSLLPGAMLYYLTGL 324
+VF +ERL+GHYG + F I NT A P+L +SL+PGA+ YY +G+
Sbjct: 804 QVFERERLNGHYGATSFAIGNTFYAIPFLLLISLIPGAIAYYPSGV 667
>gb|CX176614.1|CX176614 C10_69-38_06.ab1 leaf inoculated with Marssonia pathogen of Populus
deltoides Populus deltoides cDNA, mRNA sequence
Length = 666
Score = 61.6 bits (128), Expect(3) = 3e-024
Identities = 23/42 (54%), Positives = 33/42 (78%)
Frame = +1 / +3
Query: 187 KVFRKERLSGHYGVSEFVISNTISATPYLAGVSLLPGAMLYY 312
+VF++ERL+GHYGV FVISNTISA P+L ++ + G + Y+
Sbjct: 540 QVFQRERLNGHYGVVAFVISNTISAMPFLIMITFISGTVCYF 665
Score = 44.6 bits (91), Expect(3) = 3e-024
Identities = 14/25 (56%), Positives = 20/25 (80%)
Frame = +1 / +3
Query: 4 GYYWMRLAIYMGIGVCLGTIFYQLG 78
GYYW+RL IY+ + VC+GTI+ +G
Sbjct: 117 GYYWLRLVIYIVVTVCIGTIYLNVG 191
Score = 43.7 bits (89), Expect(3) = 3e-024
Identities = 17/31 (54%), Positives = 22/31 (70%)
Frame = +1 / +1
Query: 100 QSRCEVIMYTIALLTFMSIGGFPSFVEDVKV 192
Q+R + +TFMSIGGFPSFVE++KV
Sbjct: 334 QARGSCASFVFGFVTFMSIGGFPSFVEEMKV 426
>gb|CX171013.1|CX171013 E12_69-102_10.ab1 leaf inoculated with Marssonia pathogen of
Populus deltoides Populus deltoides cDNA, mRNA sequence
Length = 668
Score = 58.8 bits (122), Expect(3) = 2e-023
Identities = 22/43 (51%), Positives = 33/43 (76%)
Frame = +1 / +3
Query: 187 KVFRKERLSGHYGVSEFVISNTISATPYLAGVSLLPGAMLYYL 315
+VF++ERL+GHYGV FVISNTISA P+ ++ + G + Y++
Sbjct: 540 QVFQRERLNGHYGVVAFVISNTISAMPFPDMITFISGTVCYFM 668
Score = 44.6 bits (91), Expect(3) = 2e-023
Identities = 14/25 (56%), Positives = 20/25 (80%)
Frame = +1 / +3
Query: 4 GYYWMRLAIYMGIGVCLGTIFYQLG 78
GYYW+RL IY+ + VC+GTI+ +G
Sbjct: 117 GYYWLRLVIYIVVTVCIGTIYLNVG 191
Score = 43.7 bits (89), Expect(3) = 2e-023
Identities = 17/31 (54%), Positives = 22/31 (70%)
Frame = +1 / +1
Query: 100 QSRCEVIMYTIALLTFMSIGGFPSFVEDVKV 192
Q+R + +TFMSIGGFPSFVE++KV
Sbjct: 334 QARGSCASFVFGFVTFMSIGGFPSFVEEMKV 426
>gb|DT493142.1|DT493142 WS02553.C21_I10 PT-MB-N-A-15 Populus trichocarpa cDNA clone
WS02553_I10 3', mRNA sequence
Length = 787
Score = 104 bits (221), Expect = 5e-022
Identities = 42/85 (49%), Positives = 55/85 (64%)
Frame = +1 / -2
Query: 256 SATPYLAGVSLLPGAMLYYLTGLSKGADHFVYFXXXXXXXXXXXXXXXXXIAAVVPDFLM 435
SA PYL +S++PGA+ YYL G KG + F+YF +A++VP+FLM
Sbjct: 783 SAFPYLLLISVIPGAITYYLPGFHKGYEQFIYFVLVLFACMMLVESLMMTVASMVPNFLM 604
Query: 436 GIIVGAGVQGVMMLNGGFFRLPNEL 510
GII GAG+Q M+L GGFFRLPN+L
Sbjct: 603 GIITGAGIQAFMVLGGGFFRLPNDL 529
Database: Populus_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:49 PM
Number of letters in database: 203,408,664
Number of sequences in database: 369,679
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 186,510,863
Number of Sequences: 369679
Number of extensions: 1891936
Number of successful extensions: 41880
Number of sequences better than 1.0e-020: 11
length of database: 67,802,888
effective HSP length: 51
effective length of database: 48,949,259
effective search space used: 5776012562
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)