TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3336950.2.1
(645 letters)
Database: Populus_nucl_with_EST.fasta
369,679 sequences; 203,408,664 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|DT474803.1|DT474803 WS0125.BR_A19 PT-GT-FL-A-3 Populus t... 171 2e-051
gb|AC149484.1| Populus trichocarpa clone Pop1-69M19, comple... 94 4e-040
gb|DN501581.1|DN501581 V006H08.5pR Populus male catkins cDN... 164 6e-040
gb|AJ774822.1|AJ774822 AJ774822 Populus euphratica shoot 3-... 148 4e-035
>gb|DT474803.1|DT474803 WS0125.BR_A19 PT-GT-FL-A-3 Populus trichocarpa cDNA clone
WS0125_A19 5', mRNA sequence
Length = 585
Score = 171 bits (368), Expect(2) = 2e-051
Identities = 75/132 (56%), Positives = 89/132 (67%)
Frame = -1 / +3
Query: 537 KRMGITSNDAAPRAVLLRFLFLLCIGCKCLASEVEATQTATLAVDASPQLARKIPDTLFG 358
K MG + +L F++ + C S E T + + R IP+TLFG
Sbjct: 120 K*MGEKMSSCKASCSVLLACFIVGLCSVCQCSAAEVDANQTANLIINGSSTRPIPETLFG 299
Query: 357 IFFEEINHAGAGGIWAELVSNRGFEAGGLHTPSNIDPWSIIGDDSSVFVETDRTSCFSRN 178
IFFEEINHAGAGGIWAELV+NRGFEAGG +TPSNI PWSIIGD SS+ V TDR+SCF RN
Sbjct: 300 IFFEEINHAGAGGIWAELVNNRGFEAGGQNTPSNIAPWSIIGDQSSLIVSTDRSSCFERN 479
Query: 177 IVALRMEVLCDN 142
VALRM+VLCD+
Sbjct: 480 KVALRMDVLCDS 515
Score = 125 bits (268), Expect(3) = 2e-037
Identities = 54/81 (66%), Positives = 64/81 (79%)
Frame = +2 / -3
Query: 143 LSQRTSILRATMFRLKHDVRSVSTNTEESSPMMDHGSMFDGVWRPPASNPLLLTSSAHMP 322
LSQ TSI RAT+FR K + RSV T ++ SPM+D G++F+GV+ PPAS P L TSSAH+P
Sbjct: 514 LSQSTSIRRATLFRSKQEERSVDTIKDD*SPMIDQGAIFEGVFWPPASKPRLFTSSAHIP 335
Query: 323 PAPAWLISSKKIPNSVSGIFR 385
PAPAW ISSKKIP SVSGI R
Sbjct: 334 PAPAWFISSKKIPKSVSGIGR 272
Score = 51.0 bits (105), Expect(2) = 2e-051
Identities = 16/18 (88%), Positives = 17/18 (94%)
Frame = -1 / +3
Query: 141 CPTGGVGVYNPGFWGMNI 88
CP GGVG+YNPGFWGMNI
Sbjct: 531 CPAGGVGIYNPGFWGMNI 584
Score = 40.0 bits (81), Expect(3) = 2e-037
Identities = 14/19 (73%), Positives = 16/19 (84%)
Frame = +2 / -3
Query: 89 MFMPQNPGL*TPTPPVGQL 145
+FMPQNPGL PTPP GQ+
Sbjct: 583 IFMPQNPGLYIPTPPAGQI 527
Score = 28.1 bits (55), Expect(3) = 2e-037
Identities = 16/44 (36%), Positives = 19/44 (43%)
Frame = +2 / -3
Query: 413 RVAVWVASTSDARHLQPMQSRNKKRRRTARGAASLLVIPIRFHL 544
+ AVW ASTS A H Q K + A L+I HL
Sbjct: 250 KFAVWFASTSAAEHWHTEQRPTMKHAKRTLQEALQLLIFSPIHL 119
>gb|AC149484.1| Populus trichocarpa clone Pop1-69M19, complete sequence
Length = 96930
Score = 94.1 bits (199), Expect(5) = 4e-040
Identities = 38/50 (76%), Positives = 42/50 (84%)
Frame = -1 / -1
Query: 291 GFEAGGLHTPSNIDPWSIIGDDSSVFVETDRTSCFSRNIVALRMEVLCDN 142
GFEAGG +TPSNI PWSIIGD SS+ V TDR+SCF RN VALRM VLCD+
Sbjct: 72342 GFEAGGQNTPSNIAPWSIIGDQSSLTVSTDRSSCFERNKVALRMHVLCDS 72193
Score = 53.3 bits (110), Expect(5) = 4e-040
Identities = 20/22 (90%), Positives = 21/22 (95%)
Frame = -1 / -3
Query: 354 FFEEINHAGAGGIWAELVSNRG 289
F +EINHAGAGGIWAELVSNRG
Sbjct: 72742 FLQEINHAGAGGIWAELVSNRG 72677
Score = 46.4 bits (95), Expect(5) = 4e-040
Identities = 14/16 (87%), Positives = 15/16 (93%)
Frame = -1 / -1
Query: 141 CPTGGVGVYNPGFWGM 94
CP GGVG+YNPGFWGM
Sbjct: 72177 CPAGGVGIYNPGFWGM 72130
Score = 43.2 bits (88), Expect(5) = 4e-040
Identities = 16/31 (51%), Positives = 27/31 (87%)
Frame = -1 / -3
Query: 93 NIEEGKVYNLVMFVKSPETTDLTVSLTSSNG 1
NIE+GK+Y +V++V+S + +++VSLTSS+G
Sbjct: 72022 NIEQGKIYKVVLYVRSLGSINVSVSLTSSDG 71930
Score = 30.9 bits (61), Expect(5) = 4e-040
Identities = 14/33 (42%), Positives = 17/33 (51%)
Frame = -1 / -2
Query: 444 SEVEATQTATLAVDASPQLARKIPDTLFGIFFE 346
S E T + + R IP+TLFGIFFE
Sbjct: 72962 SAAEVDAYQTAKLIVNASSGRPIPETLFGIFFE 72864
>gb|DN501581.1|DN501581 V006H08.5pR Populus male catkins cDNA library Populus trichocarpa
cDNA clone V006H08 5', mRNA sequence
Length = 508
Score = 164 bits (352), Expect = 6e-040
Identities = 71/130 (54%), Positives = 86/130 (66%)
Frame = -1 / +2
Query: 537 KRMGITSNDAAPRAVLLRFLFLLCIGCKCLASEVEATQTATLAVDASPQLARKIPDTLFG 358
K MG + +L F++ + C S E T + + R IP+TLFG
Sbjct: 119 K*MGEKMSSCKASCSVLLACFIVGLCSVCQCSAAEVDANQTANLIINGSSTRPIPETLFG 298
Query: 357 IFFEEINHAGAGGIWAELVSNRGFEAGGLHTPSNIDPWSIIGDDSSVFVETDRTSCFSRN 178
IFFEEINHAGAGGIWAELV+NRGFE+GG +TPSNI PWS+IGD SS+ V TDR+SCF RN
Sbjct: 299 IFFEEINHAGAGGIWAELVNNRGFESGGQNTPSNIAPWSLIGDQSSLIVSTDRSSCFERN 478
Query: 177 IVALRMEVLC 148
ALRM+VLC
Sbjct: 479 NAALRMDVLC 508
Score = 116 bits (247), Expect(2) = 5e-028
Identities = 50/79 (63%), Positives = 59/79 (74%)
Frame = +2 / -2
Query: 149 QRTSILRATMFRLKHDVRSVSTNTEESSPMMDHGSMFDGVWRPPASNPLLLTSSAHMPPA 328
Q TSI RA +FR K + RSV T ++ SPM D G++F+GV+ PP S P L TSSAH+PPA
Sbjct: 507 QSTSIRRAALFRSKQEERSVDTIKDD*SPMRDQGAIFEGVFWPPDSKPRLFTSSAHIPPA 328
Query: 329 PAWLISSKKIPNSVSGIFR 385
PAW ISSKKIP SVSGI R
Sbjct: 327 PAWFISSKKIPKSVSGIGR 271
Score = 28.1 bits (55), Expect(2) = 5e-028
Identities = 16/44 (36%), Positives = 19/44 (43%)
Frame = +2 / -2
Query: 413 RVAVWVASTSDARHLQPMQSRNKKRRRTARGAASLLVIPIRFHL 544
+ AVW ASTS A H Q K + A L+I HL
Sbjct: 249 KFAVWFASTSAAEHWHTEQRPTMKHAKRTLQEALQLLIFSPIHL 118
>gb|AJ774822.1|AJ774822 AJ774822 Populus euphratica shoot 3-6 months Populus euphratica
cDNA clone P0000300024F09F1, mRNA sequence
Length = 538
Score = 148 bits (317), Expect = 4e-035
Identities = 65/119 (54%), Positives = 77/119 (64%)
Frame = -1 / +2
Query: 537 KRMGITSNDAAPRAVLLRFLFLLCIGCKCLASEVEATQTATLAVDASPQLARKIPDTLFG 358
K MG + +L F++ + C S E T + + R IP+TLFG
Sbjct: 182 K*MGEKMSSCKASCSVLLVCFIVGLCSVCPCSAAEVDANQTANLIINGSSTRPIPETLFG 361
Query: 357 IFFEEINHAGAGGIWAELVSNRGFEAGGLHTPSNIDPWSIIGDDSSVFVETDRTSCFSR 181
IFFEEINHAGAGGIWAELV+NRGFEAGG +TPSNI PWSIIGD SS+ V TDR+SCF R
Sbjct: 362 IFFEEINHAGAGGIWAELVNNRGFEAGGQNTPSNIAPWSIIGDQSSLIVSTDRSSCFER 538
Score = 105 bits (223), Expect(2) = 2e-024
Identities = 44/68 (64%), Positives = 53/68 (77%)
Frame = +2 / -2
Query: 182 RLKHDVRSVSTNTEESSPMMDHGSMFDGVWRPPASNPLLLTSSAHMPPAPAWLISSKKIP 361
R K + RSV T ++ SPM+D G++F+GV+ PPAS P L TSSAH+PPAPAW ISSKKIP
Sbjct: 537 RSKQEERSVDTIKDD*SPMIDQGAIFEGVFWPPASKPRLFTSSAHIPPAPAWFISSKKIP 358
Query: 362 NSVSGIFR 385
SVSGI R
Sbjct: 357 KSVSGIGR 334
Score = 27.2 bits (53), Expect(2) = 2e-024
Identities = 16/44 (36%), Positives = 19/44 (43%)
Frame = +2 / -2
Query: 413 RVAVWVASTSDARHLQPMQSRNKKRRRTARGAASLLVIPIRFHL 544
+ AVW ASTS A H Q K + A L+I HL
Sbjct: 312 KFAVWFASTSAAEHGHTEQRPTMKHTKRTLQEALQLLIFSPIHL 181
Database: Populus_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:49 PM
Number of letters in database: 203,408,664
Number of sequences in database: 369,679
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 350,510,934
Number of Sequences: 369679
Number of extensions: 5314341
Number of successful extensions: 69003
Number of sequences better than 1.0e-020: 4
length of database: 67,802,888
effective HSP length: 51
effective length of database: 48,949,259
effective search space used: 7978729217
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)