TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3276099.2.1
(545 letters)
Database: Populus_nucl_with_EST.fasta
369,679 sequences; 203,408,664 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AJ773837.1|AJ773837 AJ773837 Populus euphratica shoot 3-... 125 3e-028
gb|DN484496.1|DN484496 I036P95.3pR Populus senescing leaves... 122 2e-027
gb|CX177922.1|CX177922 G03_45-82_13.ab1 leaf inoculated wit... 105 2e-022
>gb|AJ773837.1|AJ773837 AJ773837 Populus euphratica shoot 3-6 months Populus euphratica
cDNA clone P0000300005F06F1, mRNA sequence
Length = 603
Score = 125 bits (267), Expect = 3e-028
Identities = 53/80 (66%), Positives = 63/80 (78%)
Frame = +3 / +2
Query: 42 FQALVHFYGCKLQVQERMTRQIAEAVYSVSHNGAMVVVEANHICMISRGIEKIRSNTATI 221
F +VHFYG KLQVQER+TR+IAE V S+ MVVVEANH CM SRGIEK S+TATI
Sbjct: 233 FTVIVHFYGFKLQVQERLTREIAETVSSLLGGDVMVVVEANHTCMFSRGIEKFGSSTATI 412
Query: 222 AVLGQFLADPSAKACFLQNV 281
AVLG+F DP+A+A FL+N+
Sbjct: 413 AVLGRFSTDPAARAMFLRNI 472
>gb|DN484496.1|DN484496 I036P95.3pR Populus senescing leaves cDNA library Populus tremula
cDNA clone I036P95 3', mRNA sequence
Length = 549
Score = 122 bits (261), Expect = 2e-027
Identities = 54/83 (65%), Positives = 64/83 (77%)
Frame = +3 / -3
Query: 33 RSHFQALVHFYGCKLQVQERMTRQIAEAVYSVSHNGAMVVVEANHICMISRGIEKIRSNT 212
+S Q++V FYG KLQVQER+TRQIAE S+ +VVVEANH CMISRGIEK S+T
Sbjct: 460 KSCSQSIVRFYGFKLQVQERLTRQIAETASSLLGGDVVVVVEANHTCMISRGIEKFESST 281
Query: 213 ATIAVLGQFLADPSAKACFLQNV 281
ATIAVLG F DP+A+A FLQN+
Sbjct: 280 ATIAVLGLFSTDPAARAKFLQNI 212
Score = 93.6 bits (198), Expect(2) = 7e-021
Identities = 48/75 (64%), Positives = 52/75 (69%)
Frame = -2 / +3
Query: 277 FCKKHALAEGSAKNWPKTAIVAVLLLIFSIPLEIMQMWLASTTTMAPLCETE*TASAICL 98
FCK ALA GS +N P TAIVAVLL FSIPLEI+Q+WLASTTT + SAICL
Sbjct: 216 FCKNLALAAGSVENRPSTAIVAVLLSNFSIPLEIIQVWLASTTTTTSPPNKDEAVSAICL 395
Query: 97 VILS*T*SLHP*K*T 53
V LS T SL P*K T
Sbjct: 396 VSLSCTWSLKP*KRT 440
Score = 26.3 bits (51), Expect(2) = 7e-021
Identities = 13/33 (39%), Positives = 19/33 (57%)
Frame = -1 / +2
Query: 449 CDILGTVPQVTNLHPRRIMLDQ*IAKNFNCWIV 351
CD L TV +T ++ ++* A NFN W+V
Sbjct: 2 CDELITVTCLTGKLCQKSNHER*QASNFNLWVV 100
>gb|CX177922.1|CX177922 G03_45-82_13.ab1 leaf inoculated with Marssonia pathogen of Populus
euramericana Populus x canadensis cDNA, mRNA sequence
Length = 701
Score = 105 bits (225), Expect = 2e-022
Identities = 47/69 (68%), Positives = 54/69 (78%)
Frame = +3 / +2
Query: 27 IDRSHFQALVHFYGCKLQVQERMTRQIAEAVYSVSHNGAMVVVEANHICMISRGIEKIRS 206
+ +S Q++VHFYG KLQVQER+TRQIAE V S+ MVVVEA H CMISRGIEK S
Sbjct: 494 LSKSLLQSIVHFYGFKLQVQERLTRQIAETVSSLLGGDVMVVVEAKHTCMISRGIEKYGS 673
Query: 207 NTATIAVLG 233
+TATIAVLG
Sbjct: 674 STATIAVLG 700
Database: Populus_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:49 PM
Number of letters in database: 203,408,664
Number of sequences in database: 369,679
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 308,293,145
Number of Sequences: 369679
Number of extensions: 4435085
Number of successful extensions: 76703
Number of sequences better than 1.0e-020: 3
length of database: 67,802,888
effective HSP length: 51
effective length of database: 48,949,259
effective search space used: 6363403670
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)