TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3115184.2.1
(642 letters)
Database: Populus_nucl_with_EST.fasta
369,679 sequences; 203,408,664 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|DT512918.1|DT512918 WS02420.B21_J19 PTxD-ICC-N-A-14 Popu... 82 7e-027
gb|CV253324.1|CV253324 WS0221.B21_J07 PTxD-ICC-A-12 Populus... 78 2e-025
gb|CV243754.1|CV243754 WS0252.B21_M05 PT-MB-N-A-15 Populus ... 82 4e-025
gb|DN486268.1|DN486268 P012D09.3pR Populus petioles cDNA li... 72 3e-024
gb|CK318807.1|CK318807 X9P03f01 Populus stem seasonal libra... 72 3e-024
gb|BU888780.1|BU888780 P012D09 Populus petioles cDNA librar... 72 3e-024
gb|DN495824.1|DN495824 P012D09.5pR Populus petioles cDNA li... 68 9e-022
>gb|DT512918.1|DT512918 WS02420.B21_J19 PTxD-ICC-N-A-14 Populus trichocarpa x Populus
deltoides cDNA clone WS02420_J19 3', mRNA sequence
Length = 891
Score = 82.2 bits (173), Expect(3) = 7e-027
Identities = 32/81 (39%), Positives = 47/81 (58%)
Frame = +3 / -1
Query: 147 ATKFLYQDVTMDNVSYPIIIDQKYCPNNICVKSGASKVAVNDVVFKNIHGTSNTPEAITL 326
A L+Q M NV PI+IDQ YCP S V ++DV +++IHG+S T A+
Sbjct: 417 ARNILFQHAVMTNVQNPIVIDQNYCPGEKGCPGQVSGVKISDVTYQDIHGSSATELAVKF 238
Query: 327 NCANNLPCQGVQLINVDIKYN 389
+C+ PC G++L +V + YN
Sbjct: 237 DCSKTNPCSGIKLEDVKLTYN 175
Score = 52.4 bits (108), Expect(3) = 7e-027
Identities = 21/40 (52%), Positives = 28/40 (70%)
Frame = +3 / -1
Query: 3 GHGISVGSLGRYKDEKDVEDVKVTGCTLAGTTNGLRIKSY 122
GHGIS+GSLG+ E V++V V T GT NG+RIK++
Sbjct: 558 GHGISIGSLGKDSQEAGVQNVTVKTTTFTGTENGVRIKTW 439
Score = 23.5 bits (45), Expect(3) = 7e-027
Identities = 9/33 (27%), Positives = 18/33 (54%)
Frame = +3 / -1
Query: 375 DIKYNRSDNKTMSVCKNAIGKSIGMAKELACV* 473
D+K ++ + C NA G + G+ + +C+*
Sbjct: 195 DVKLTYNNQPAEASCSNAGGVASGVVQPTSCL* 97
>gb|CV253324.1|CV253324 WS0221.B21_J07 PTxD-ICC-A-12 Populus trichocarpa x Populus
deltoides cDNA clone WS0221_J07 3', mRNA sequence
Length = 722
Score = 78.0 bits (164), Expect(3) = 2e-025
Identities = 31/81 (38%), Positives = 46/81 (56%)
Frame = +3 / -2
Query: 147 ATKFLYQDVTMDNVSYPIIIDQKYCPNNICVKSGASKVAVNDVVFKNIHGTSNTPEAITL 326
A L+Q M NV PI+IDQ YCP S V ++DV +++IHG+S T A+
Sbjct: 571 ARNILFQHAVMTNVQNPIVIDQNYCPGEKGCPGQVSGVKISDVTYQDIHGSSATELAVKF 392
Query: 327 NCANNLPCQGVQLINVDIKYN 389
+C+ PC G++L +V + N
Sbjct: 391 DCSKTNPCSGIKLEDVKLTCN 329
Score = 52.4 bits (108), Expect(3) = 2e-025
Identities = 21/40 (52%), Positives = 28/40 (70%)
Frame = +3 / -2
Query: 3 GHGISVGSLGRYKDEKDVEDVKVTGCTLAGTTNGLRIKSY 122
GHGIS+GSLG+ E V++V V T GT NG+RIK++
Sbjct: 712 GHGISIGSLGKDSQEAGVQNVTVKTTTFTGTENGVRIKTW 593
Score = 23.1 bits (44), Expect(3) = 2e-025
Identities = 9/33 (27%), Positives = 18/33 (54%)
Frame = +3 / -2
Query: 375 DIKYNRSDNKTMSVCKNAIGKSIGMAKELACV* 473
D+K ++ + C NA G + G+ + +C+*
Sbjct: 349 DVKLTCNNQPAEASCSNAGGVASGVVQPTSCL* 251
>gb|CV243754.1|CV243754 WS0252.B21_M05 PT-MB-N-A-15 Populus trichocarpa cDNA clone
WS0252_M05 3', mRNA sequence
Length = 928
Score = 82.2 bits (173), Expect(2) = 4e-025
Identities = 34/78 (43%), Positives = 51/78 (65%)
Frame = +3 / -3
Query: 147 ATKFLYQDVTMDNVSYPIIIDQKYCPNNICVKSGASKVAVNDVVFKNIHGTSNTPEAITL 326
AT +Q++ M+NVS PIIIDQ YC ++ + S V V ++ F+ I GTS T EAI
Sbjct: 716 ATNITFQNIFMENVSNPIIIDQYYCDAHVPCANQTSAVKVENISFRRIKGTSATEEAIKF 537
Query: 327 NCANNLPCQGVQLINVDI 380
C+++LPC+G+ L +V +
Sbjct: 536 ACSDDLPCKGLYLEDVQL 483
Score = 52.4 bits (108), Expect(2) = 4e-025
Identities = 21/41 (51%), Positives = 28/41 (68%)
Frame = +3 / -3
Query: 3 GHGISVGSLGRYKDEKDVEDVKVTGCTLAGTTNGLRIKSYE 125
GHGIS+GSLG+ V DV V G L+ T NG+RIK+++
Sbjct: 854 GHGISIGSLGKSNSSSLVRDVMVDGAFLSNTDNGVRIKTWQ 732
>gb|DN486268.1|DN486268 P012D09.3pR Populus petioles cDNA library Populus tremula cDNA
clone P012D09 3', mRNA sequence
Length = 768
Score = 72.1 bits (151), Expect(2) = 3e-024
Identities = 28/74 (37%), Positives = 48/74 (64%)
Frame = +3 / -2
Query: 162 YQDVTMDNVSYPIIIDQKYCPNNICVKSGASKVAVNDVVFKNIHGTSNTPEAITLNCANN 341
+Q++ M+ V+ PIIIDQ YC + + S + V +VV+KNI GTS + A+ +C+
Sbjct: 515 FQNIEMNKVANPIIIDQNYCDQDKPCRQQKSALQVKNVVYKNIKGTSASEVAMRFDCSKA 336
Query: 342 LPCQGVQLINVDIK 383
PCQG+ L +++++
Sbjct: 335 YPCQGILLQDINLE 294
Score = 59.3 bits (123), Expect(2) = 3e-024
Identities = 23/52 (44%), Positives = 34/52 (65%)
Frame = +3 / -2
Query: 3 GHGISVGSLGRYKDEKDVEDVKVTGCTLAGTTNGLRIKSYEDSKSSLKATKF 158
GHGIS+GSLG + + V +V V G L+GT NG+RIK+++ ++ KF
Sbjct: 668 GHGISIGSLGVHDSKDHVSEVTVNGAKLSGTANGVRIKTWQGGSGNVSNIKF 513
>gb|CK318807.1|CK318807 X9P03f01 Populus stem seasonal library Populus deltoides cDNA, mRNA
sequence
Length = 736
Score = 72.1 bits (151), Expect(2) = 3e-024
Identities = 28/74 (37%), Positives = 48/74 (64%)
Frame = +3 / +2
Query: 162 YQDVTMDNVSYPIIIDQKYCPNNICVKSGASKVAVNDVVFKNIHGTSNTPEAITLNCANN 341
+Q++ M+ V+ PIIIDQ YC + K S + V +V++KNI GTS + A+ +C+
Sbjct: 275 FQNIEMNKVTNPIIIDQNYCDQDKPCKQQKSALQVKNVMYKNIKGTSASEVAMRFDCSKA 454
Query: 342 LPCQGVQLINVDIK 383
PCQG+ L +++++
Sbjct: 455 YPCQGILLQDINLE 496
Score = 59.3 bits (123), Expect(2) = 3e-024
Identities = 23/52 (44%), Positives = 34/52 (65%)
Frame = +3 / +2
Query: 3 GHGISVGSLGRYKDEKDVEDVKVTGCTLAGTTNGLRIKSYEDSKSSLKATKF 158
GHGIS+GSLG + + V V V+G L+GT NG+RIK+++ ++ KF
Sbjct: 122 GHGISIGSLGAHDSKDHVSGVTVSGAKLSGTANGVRIKTWQGGSGNVSNIKF 277
>gb|BU888780.1|BU888780 P012D09 Populus petioles cDNA library Populus tremula cDNA 5 prime,
mRNA sequence
Length = 728
Score = 72.1 bits (151), Expect(2) = 3e-024
Identities = 28/74 (37%), Positives = 48/74 (64%)
Frame = +3 / +3
Query: 162 YQDVTMDNVSYPIIIDQKYCPNNICVKSGASKVAVNDVVFKNIHGTSNTPEAITLNCANN 341
+Q++ M+ V+ PIIIDQ YC + + S + V +VV+KNI GTS + A+ +C+
Sbjct: 390 FQNIEMNKVANPIIIDQNYCDQDKPCRQQKSALQVKNVVYKNIKGTSASEVAMRFDCSKA 569
Query: 342 LPCQGVQLINVDIK 383
PCQG+ L +++++
Sbjct: 570 YPCQGILLQDINLE 611
Score = 59.3 bits (123), Expect(2) = 3e-024
Identities = 23/52 (44%), Positives = 34/52 (65%)
Frame = +3 / +3
Query: 3 GHGISVGSLGRYKDEKDVEDVKVTGCTLAGTTNGLRIKSYEDSKSSLKATKF 158
GHGIS+GSLG + + V +V V G L+GT NG+RIK+++ ++ KF
Sbjct: 237 GHGISIGSLGVHDSKDHVSEVTVNGAKLSGTANGVRIKTWQGGSGNVSNIKF 392
>gb|DN495824.1|DN495824 P012D09.5pR Populus petioles cDNA library Populus tremula cDNA
clone P012D09 5', mRNA sequence
Length = 820
Score = 68.4 bits (143), Expect(2) = 9e-022
Identities = 27/74 (36%), Positives = 47/74 (63%)
Frame = +3 / +2
Query: 162 YQDVTMDNVSYPIIIDQKYCPNNICVKSGASKVAVNDVVFKNIHGTSNTPEAITLNCANN 341
+Q++ M+ V+ PIIIDQ YC + + S + V +VV+KNI GTS + A+ +C+
Sbjct: 392 FQNIEMNKVANPIIIDQNYCDQDKPCRQQKSALQVKNVVYKNIKGTSASEVAMRFDCSKA 571
Query: 342 LPCQGVQLINVDIK 383
PCQ + L +++++
Sbjct: 572 YPCQRILLQDINLE 613
Score = 54.7 bits (113), Expect(2) = 9e-022
Identities = 22/52 (42%), Positives = 33/52 (63%)
Frame = +3 / +2
Query: 3 GHGISVGSLGRYKDEKDVEDVKVTGCTLAGTTNGLRIKSYEDSKSSLKATKF 158
GHGIS+GSL + + V +V V G L+GT NG+RIK+++ ++ KF
Sbjct: 239 GHGISIGSL*VHDSKDHVSEVTVNGAKLSGTANGVRIKTWQGGSGNVSNIKF 394
Database: Populus_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:49 PM
Number of letters in database: 203,408,664
Number of sequences in database: 369,679
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 193,676,094
Number of Sequences: 369679
Number of extensions: 1788258
Number of successful extensions: 26040
Number of sequences better than 1.0e-020: 7
length of database: 67,802,888
effective HSP length: 51
effective length of database: 48,949,259
effective search space used: 7929779958
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)