TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3054736.2.1
(725 letters)
Database: Populus_nucl_with_EST.fasta
369,679 sequences; 203,408,664 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CK092473.1|CK092473 G084P78.3pR Populus tension wood cDN... 206 1e-052
gb|CK096470.1|CK096470 UB15CPF09.3pR Populus active cambium... 183 1e-045
gb|CX655011.1|CX655011 PO01003G06 Poplar SC cDNA library Po... 183 1e-045
gb|CV255308.1|CV255308 WS02412.B21_K12 PTxD-ICC-N-A-14 Popu... 181 4e-045
gb|DT517578.1|DT517578 WS02434.B21_L09 PTxD-ICC-N-A-14 Popu... 181 4e-045
gb|CA931213.1|CA931213 MTU2TA.P4.A06 Aspen apex cDNA Librar... 168 3e-041
gb|CA931239.1|CA931239 MTU2TA.P4.E07 Aspen apex cDNA Librar... 168 3e-041
gb|CX655147.1|CX655147 PO02030E06 Poplar SC cDNA library Po... 106 1e-022
>gb|CK092473.1|CK092473 G084P78.3pR Populus tension wood cDNA library Populus tremula x
Populus tremuloides cDNA clone G084P78 3', mRNA sequence
Length = 726
Score = 206 bits (445), Expect = 1e-052
Identities = 79/118 (66%), Positives = 97/118 (82%)
Frame = +3 / -3
Query: 3 VVNDPNLKWYESSWELLGNEYFMHASGSLYALSSEVVEALATAKSDSLRMFDYEDVTIGA 182
V+ DP +KWYE+S L+GNEYF+HA G +Y LS+EVV +LA+A+++SLRMF EDVTIG+
Sbjct: 589 VITDPKMKWYETSGHLIGNEYFLHAYGPIYVLSAEVVASLASARNNSLRMFSNEDVTIGS 410
Query: 183 WMLAMNVKHEDNRAMCDSVCTPTSIAVWDSKKCSGTCNVADKIKQLHNTTVCSKSPTL 356
WMLAMNV HEDNRA+CD CTPTSIAVWD KCSG CN A ++K+LH +CSKSPTL
Sbjct: 409 WMLAMNVYHEDNRAICDPRCTPTSIAVWDIPKCSGLCNPASRMKELHKIDMCSKSPTL 236
Score = 144 bits (308), Expect = 8e-034
Identities = 69/120 (57%), Positives = 80/120 (66%)
Frame = -2 / +3
Query: 361 GGNVGLFEHTVVLWSCFILSATLQVPEHFLLSHTAMEVGVHTESHIARLSSCFTFIASIH 182
GG+VGL EH +L S FI A L PEH +S TA++VGVH SH+ARLSS TFIASIH
Sbjct: 231 GGSVGLLEHISIL*SSFIRLAGLHKPEHLGISQTAIDVGVHRGSHMARLSS**TFIASIH 410
Query: 181 APIVTSS*SNILKLSLFAVARASTTSEERAYSEPDACMKYSLPNSSQELSYHFKFGSLTT 2
P+VTSS NILKL A A +TTS ER Y P AC KYS P ++SYHF GS+ T
Sbjct: 411 DPMVTSSLLNILKLLFLADANEATTSAERTYIGP*ACRKYSFPIRCPDVSYHFILGSVIT 590
>gb|CK096470.1|CK096470 UB15CPF09.3pR Populus active cambium cDNA library Populus tremula
cDNA clone UB15CPF09 3', mRNA sequence
Length = 774
Score = 183 bits (394), Expect = 1e-045
Identities = 71/118 (60%), Positives = 86/118 (72%)
Frame = +3 / -3
Query: 3 VVNDPNLKWYESSWELLGNEYFMHASGSLYALSSEVVEALATAKSDSLRMFDYEDVTIGA 182
V DP LKWYE LLG EYF+HA G +YALS++VV +L + +S RMF EDVTIGA
Sbjct: 508 VFTDPKLKWYEPLSYLLGTEYFLHAYGPIYALSADVVASLVALRDNSFRMFSNEDVTIGA 329
Query: 183 WMLAMNVKHEDNRAMCDSVCTPTSIAVWDSKKCSGTCNVADKIKQLHNTTVCSKSPTL 356
W+LAMNV HEDNRA+C CTP+SIAVWD KCSG CN D++ +LH CSK+PT+
Sbjct: 328 WILAMNVNHEDNRALCSPECTPSSIAVWDIPKCSGLCNPEDRLLELHQQESCSKNPTM 155
Score = 121 bits (259), Expect = 5e-027
Identities = 61/117 (52%), Positives = 71/117 (60%)
Frame = -2 / +3
Query: 352 VGLFEHTVVLWSCFILSATLQVPEHFLLSHTAMEVGVHTESHIARLSSCFTFIASIHAPI 173
VG FE WS LS+ LQ PEH +S TA++ GVH+ H A LSS TFIA IHAPI
Sbjct: 159 VGFFEQLSC*WSSNNLSSGLQSPEHLGMSQTAIDDGVHSGEHSALLSS*LTFIARIHAPI 338
Query: 172 VTSS*SNILKLSLFAVARASTTSEERAYSEPDACMKYSLPNSSQELSYHFKFGSLTT 2
VTSS NI KL + +TTS ERAY P A KYS+P+ SYH FGS+ T
Sbjct: 339 VTSSLLNIRKLLSLRATKLATTSAERAYIGPYA*RKYSVPSK*DSGSYHLSFGSVKT 509
>gb|CX655011.1|CX655011 PO01003G06 Poplar SC cDNA library Populus alba x Populus tremula
var. glandulosa cDNA clone PO01003G06 5', mRNA sequence
Length = 693
Score = 183 bits (394), Expect = 1e-045
Identities = 71/118 (60%), Positives = 86/118 (72%)
Frame = +3 / +3
Query: 3 VVNDPNLKWYESSWELLGNEYFMHASGSLYALSSEVVEALATAKSDSLRMFDYEDVTIGA 182
V DP LKWYE LLG EYF+HA G +YALS++VV +L + +S RMF EDVTIGA
Sbjct: 258 VFTDPKLKWYEPLSYLLGTEYFLHAYGPIYALSADVVASLVALRDNSFRMFSNEDVTIGA 437
Query: 183 WMLAMNVKHEDNRAMCDSVCTPTSIAVWDSKKCSGTCNVADKIKQLHNTTVCSKSPTL 356
W+LAMNV HEDNRA+C CTP+SIAVWD KCSG CN D++ +LH CSK+PT+
Sbjct: 438 WILAMNVNHEDNRALCSPECTPSSIAVWDIPKCSGLCNPEDRLLELHQQESCSKNPTM 611
Score = 121 bits (259), Expect = 5e-027
Identities = 61/117 (52%), Positives = 71/117 (60%)
Frame = -2 / -3
Query: 352 VGLFEHTVVLWSCFILSATLQVPEHFLLSHTAMEVGVHTESHIARLSSCFTFIASIHAPI 173
VG FE WS LS+ LQ PEH +S TA++ GVH+ H A LSS TFIA IHAPI
Sbjct: 607 VGFFEQLSC*WSSNNLSSGLQSPEHLGMSQTAIDDGVHSGEHSALLSS*LTFIARIHAPI 428
Query: 172 VTSS*SNILKLSLFAVARASTTSEERAYSEPDACMKYSLPNSSQELSYHFKFGSLTT 2
VTSS NI KL + +TTS ERAY P A KYS+P+ SYH FGS+ T
Sbjct: 427 VTSSLLNIRKLLSLRATKLATTSAERAYIGPYA*RKYSVPSK*DSGSYHLSFGSVKT 257
>gb|CV255308.1|CV255308 WS02412.B21_K12 PTxD-ICC-N-A-14 Populus trichocarpa x Populus
deltoides cDNA clone WS02412_K12 3', mRNA sequence
Length = 736
Score = 181 bits (390), Expect = 4e-045
Identities = 71/118 (60%), Positives = 86/118 (72%)
Frame = +3 / -2
Query: 3 VVNDPNLKWYESSWELLGNEYFMHASGSLYALSSEVVEALATAKSDSLRMFDYEDVTIGA 182
V DP LKWYE LLG EYF+HA G +YALS++VV +L +++S RMF EDVTIGA
Sbjct: 645 VFTDPKLKWYEPLSYLLGKEYFLHAYGPIYALSADVVASLVALRNNSFRMFSNEDVTIGA 466
Query: 183 WMLAMNVKHEDNRAMCDSVCTPTSIAVWDSKKCSGTCNVADKIKQLHNTTVCSKSPTL 356
W+LAMNV HEDNRA+C CTP+SIAVWD KCSG CN ++ +LH CSKSPT+
Sbjct: 465 WILAMNVNHEDNRALCSPECTPSSIAVWDIPKCSGLCNPEARLLELHQQESCSKSPTM 292
Score = 122 bits (261), Expect = 2e-027
Identities = 61/117 (52%), Positives = 71/117 (60%)
Frame = -2 / +2
Query: 352 VGLFEHTVVLWSCFILSATLQVPEHFLLSHTAMEVGVHTESHIARLSSCFTFIASIHAPI 173
VGLFE WS L++ LQ PEH +S TA++ GVH+ H A LSS TFIA IHAPI
Sbjct: 296 VGLFEQLSC*WSSNNLASGLQSPEHLGMSQTAIDDGVHSGEHSALLSS*LTFIARIHAPI 475
Query: 172 VTSS*SNILKLSLFAVARASTTSEERAYSEPDACMKYSLPNSSQELSYHFKFGSLTT 2
VTSS NI KL + +TTS ERAY P A KYS P+ SYH FGS+ T
Sbjct: 476 VTSSLLNIRKLLFLRATKLATTSAERAYIGPYA*RKYSFPSK*DSGSYHLSFGSVKT 646
>gb|DT517578.1|DT517578 WS02434.B21_L09 PTxD-ICC-N-A-14 Populus trichocarpa x Populus
deltoides cDNA clone WS02434_L09 3', mRNA sequence
Length = 911
Score = 181 bits (390), Expect = 4e-045
Identities = 71/118 (60%), Positives = 86/118 (72%)
Frame = +3 / -1
Query: 3 VVNDPNLKWYESSWELLGNEYFMHASGSLYALSSEVVEALATAKSDSLRMFDYEDVTIGA 182
V DP LKWYE LLG EYF+HA G +YALS++VV +L +++S RMF EDVTIGA
Sbjct: 662 VFTDPKLKWYEPLSYLLGKEYFLHAYGPIYALSADVVASLVALRNNSFRMFSNEDVTIGA 483
Query: 183 WMLAMNVKHEDNRAMCDSVCTPTSIAVWDSKKCSGTCNVADKIKQLHNTTVCSKSPTL 356
W+LAMNV HEDNRA+C CTP+SIAVWD KCSG CN ++ +LH CSKSPT+
Sbjct: 482 WILAMNVNHEDNRALCSPECTPSSIAVWDIPKCSGLCNPEARLLELHQQESCSKSPTM 309
Score = 122 bits (261), Expect = 2e-027
Identities = 61/117 (52%), Positives = 71/117 (60%)
Frame = -2 / +1
Query: 352 VGLFEHTVVLWSCFILSATLQVPEHFLLSHTAMEVGVHTESHIARLSSCFTFIASIHAPI 173
VGLFE WS L++ LQ PEH +S TA++ GVH+ H A LSS TFIA IHAPI
Sbjct: 313 VGLFEQLSC*WSSNNLASGLQSPEHLGMSQTAIDDGVHSGEHSALLSS*LTFIARIHAPI 492
Query: 172 VTSS*SNILKLSLFAVARASTTSEERAYSEPDACMKYSLPNSSQELSYHFKFGSLTT 2
VTSS NI KL + +TTS ERAY P A KYS P+ SYH FGS+ T
Sbjct: 493 VTSSLLNIRKLLFLRATKLATTSAERAYIGPYA*RKYSFPSK*DSGSYHLSFGSVKT 663
>gb|CA931213.1|CA931213 MTU2TA.P4.A06 Aspen apex cDNA Library Populus tremuloides cDNA,
mRNA sequence
Length = 376
Score = 168 bits (362), Expect = 3e-041
Identities = 66/97 (68%), Positives = 80/97 (82%)
Frame = +3 / -1
Query: 66 FMHASGSLYALSSEVVEALATAKSDSLRMFDYEDVTIGAWMLAMNVKHEDNRAMCDSVCT 245
F+HA G +Y LS+EVV +LA+A+++SLRMF EDVTIG+WMLAMNV HEDNRA+CD CT
Sbjct: 376 FLHAYGPIYVLSAEVVASLASARNNSLRMFSNEDVTIGSWMLAMNVYHEDNRAICDPRCT 197
Query: 246 PTSIAVWDSKKCSGTCNVADKIKQLHNTTVCSKSPTL 356
PTSIAVWD KCSG CN A ++K+LH +CSKSPTL
Sbjct: 196 PTSIAVWDIPKCSGLCNPASRMKELHKIDMCSKSPTL 86
Score = 116 bits (248), Expect = 2e-025
Identities = 58/97 (59%), Positives = 65/97 (67%)
Frame = -2 / +3
Query: 361 GGNVGLFEHTVVLWSCFILSATLQVPEHFLLSHTAMEVGVHTESHIARLSSCFTFIASIH 182
GG+VGL EH +L S FI A L PEH +S TA+EVGVH SH+A LSS TFIASIH
Sbjct: 81 GGSVGLLEHISIL*SSFIRLAGLHKPEHLGISQTAIEVGVHRGSHMALLSS**TFIASIH 260
Query: 181 APIVTSS*SNILKLSLFAVARASTTSEERAYSEPDAC 71
P+VTSS NILKL A A +TTS ER Y P AC
Sbjct: 261 DPMVTSSLLNILKLLFLADANEATTSAERTYIGP*AC 371
>gb|CA931239.1|CA931239 MTU2TA.P4.E07 Aspen apex cDNA Library Populus tremuloides cDNA,
mRNA sequence
Length = 376
Score = 168 bits (362), Expect = 3e-041
Identities = 66/97 (68%), Positives = 80/97 (82%)
Frame = +3 / -1
Query: 66 FMHASGSLYALSSEVVEALATAKSDSLRMFDYEDVTIGAWMLAMNVKHEDNRAMCDSVCT 245
F+HA G +Y LS+EVV +LA+A+++SLRMF EDVTIG+WMLAMNV HEDNRA+CD CT
Sbjct: 376 FLHAYGPIYVLSAEVVASLASARNNSLRMFSNEDVTIGSWMLAMNVYHEDNRAICDPRCT 197
Query: 246 PTSIAVWDSKKCSGTCNVADKIKQLHNTTVCSKSPTL 356
PTSIAVWD KCSG CN A ++K+LH +CSKSPTL
Sbjct: 196 PTSIAVWDIPKCSGLCNPASRMKELHKIDMCSKSPTL 86
Score = 116 bits (248), Expect = 2e-025
Identities = 58/97 (59%), Positives = 65/97 (67%)
Frame = -2 / +3
Query: 361 GGNVGLFEHTVVLWSCFILSATLQVPEHFLLSHTAMEVGVHTESHIARLSSCFTFIASIH 182
GG+VGL EH +L S FI A L PEH +S TA+EVGVH SH+A LSS TFIASIH
Sbjct: 81 GGSVGLLEHISIL*SSFIRLAGLHKPEHLGISQTAIEVGVHRGSHMALLSS**TFIASIH 260
Query: 181 APIVTSS*SNILKLSLFAVARASTTSEERAYSEPDAC 71
P+VTSS NILKL A A +TTS ER Y P AC
Sbjct: 261 DPMVTSSLLNILKLLFLADANEATTSAERTYIGP*AC 371
>gb|CX655147.1|CX655147 PO02030E06 Poplar SC cDNA library Populus alba x Populus tremula
var. glandulosa cDNA clone PO02030E06 5', mRNA sequence
Length = 600
Score = 106 bits (227), Expect = 1e-022
Identities = 44/71 (61%), Positives = 51/71 (71%)
Frame = +3 / +3
Query: 3 VVNDPNLKWYESSWELLGNEYFMHASGSLYALSSEVVEALATAKSDSLRMFDYEDVTIGA 182
V DP LKWYE LLG EYF HA G +YALS++VV +L +++S RM EDVTIGA
Sbjct: 387 VFTDPKLKWYEPLSYLLGKEYFYHAYGPIYALSADVVASLVVLRNNSFRMSSNEDVTIGA 566
Query: 183 WMLAMNVKHED 215
WMLAMNV HED
Sbjct: 567 WMLAMNVNHED 599
Database: Populus_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:49 PM
Number of letters in database: 203,408,664
Number of sequences in database: 369,679
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 391,005,889
Number of Sequences: 369679
Number of extensions: 5553288
Number of successful extensions: 49493
Number of sequences better than 1.0e-020: 8
length of database: 67,802,888
effective HSP length: 52
effective length of database: 48,579,580
effective search space used: 9181540620
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)