TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2950292.2.1
(1205 letters)
Database: Populus_nucl_with_EST.fasta
369,679 sequences; 203,408,664 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CV261988.1|CV261988 WS02018.B21_F08 PTxN-IB-N-A-11 Popul... 243 1e-088
gb|CX168823.1|CX168823 E07_69-80_09.ab1 leaf inoculated wit... 211 1e-085
gb|CN518663.1|CN518663 GQ0102.B3_A24 GQ010 Populus trichoca... 242 3e-063
gb|BI068874.1|BI068874 C028P46U Populus strain T89 leaves P... 126 6e-029
gb|BP924384.1|BP924384 BP924384 full-length enriched poplar... 118 6e-026
>gb|CV261988.1|CV261988 WS02018.B21_F08 PTxN-IB-N-A-11 Populus trichocarpa x Populus nigra
cDNA clone WS02018_F08 3', mRNA sequence
Length = 876
Score = 243 bits (526), Expect(3) = 1e-088
Identities = 94/115 (81%), Positives = 106/115 (92%)
Frame = +2 / -1
Query: 488 EEPNSHVVEQRVEWLYAFDVHCNSFFPAFVILYVVQYFLSPLLVAHGFFPALLSNLLFVV 667
E PNSHVVEQRVEWLYAFDVHCNSFFP FV+LYV+ YFLSP+LVAHGF P LLSNLLF+V
Sbjct: 663 EAPNSHVVEQRVEWLYAFDVHCNSFFPMFVMLYVIHYFLSPILVAHGFIPVLLSNLLFMV 484
Query: 668 AISYYHYLNFLGYDVLPFLDRTTFFLYPIGLVIILSPLMILIGFNPTRYFLSLYF 832
A SYYHYLNFLGY VLPFL+RTTFFLYPIGLVI+LSP++IL GFNP+RY +++YF
Sbjct: 483 AASYYHYLNFLGYYVLPFLERTTFFLYPIGLVIVLSPILILSGFNPSRYLMNVYF 319
Score = 155 bits (332), Expect(2) = 2e-049
Identities = 77/118 (65%), Positives = 81/118 (68%)
Frame = -3 / +2
Query: 840 L*PKYKLRKYLVGLNPISIISGDRMMTRPIGYRKNVVLSRNGRTSYPRKFR****EIATT 661
L* KY KYL GL P+ I GDR MTRPIG RKNVVLSR G T P+K R*** E AT
Sbjct: 311 L*LKYTFIKYLEGLKPLKIKIGDRTMTRPIG*RKNVVLSRKGNT**PKKLR***YEAATM 490
Query: 660 KSRLDNKAGKKPCATNRGDRKYCTTYRMTNAGKNELQ*TSNA*SHSTRCSTTWLLGSS 487
SR D+ G KP AT GDRK C T MTN GK ELQ TSNA SHSTRCSTT L G+S
Sbjct: 491 NSRFDSNTGMKPWATRMGDRK*CIT*SMTNIGKKELQCTSNAYSHSTRCSTT*LFGAS 664
Score = 81.7 bits (172), Expect(2) = 5e-021
Identities = 43/114 (37%), Positives = 68/114 (59%)
Frame = -2 / +1
Query: 838 LTEVQA*KVSCRIKPY*YHKWRQDDDETNRIQEECCXXXXXXXXXXXKVQVVIIRNCHHE 659
LT+V +S RI+ * R D+D+T+ I+E+CC KV+V+I+R CHHE
Sbjct: 313 LTKVHIH*IS*RIEAT*NQNRR*DNDKTDWIEEKCCSLQEGQHIIA*KVEVMIVRGCHHE 492
Query: 658 E*IR*QSWKEAMRDQ*RRQKILHHI*DDKRRKERVTMNVKCIEPLHALLNNMAV 497
+ +R Q E M + Q+I+++I DK +ER+ +++K I P + LLNNM +
Sbjct: 493 QQVRQQYRYETMGN*NG*QEIMYNIKHDKHWEERIAVHIKRI*PFNPLLNNMTI 654
Score = 76.7 bits (161), Expect(3) = 1e-088
Identities = 31/49 (63%), Positives = 33/49 (67%)
Frame = +2 / -3
Query: 281 KNQWARDDPAFVVILILFLVFATSAYCAAYGESASHAALTITSVVFLHF 427
KNQWARDDPAFVVI L L A AYCAAY SA HA + SV+ HF
Sbjct: 874 KNQWARDDPAFVVISSLLLAVAALAYCAAYDHSAGHAVFVVISVLLFHF 728
Score = 62.0 bits (129), Expect(2) = 2e-049
Identities = 27/49 (55%), Positives = 34/49 (69%)
Frame = -3 / +3
Query: 429 KKWRNTTEVIVRAACEALSPYAAQ*ADVANTRKRIRITTNAGSSRAHWF 283
KK ++ TE+ + AC AL YAAQ*A A ++R+ ITT AGSSRAH F
Sbjct: 726 KK*KSNTEITTKTACPALWSYAAQ*AKAATAKRRLEITTKAGSSRAH*F 872
Score = 46.4 bits (95), Expect(3) = 1e-088
Identities = 18/26 (69%), Positives = 19/26 (73%)
Frame = +2 / -1
Query: 416 FLHFLFAGIVLATLCWFLTNSYLREE 493
F FL G LAT CWFLTN+YLREE
Sbjct: 738 FFIFLITGAGLATCCWFLTNAYLREE 661
Score = 40.0 bits (81), Expect(2) = 5e-021
Identities = 20/53 (37%), Positives = 34/53 (64%)
Frame = -2 / +2
Query: 439 PSKQEMEKHNRSDC*SSM*GTLSICCTVS*CCKHEEKNQNNNKCRIITGPLVL 281
P ++M+K R++ +SM T+ I T+S C +++ +NNK R+IT PL+L
Sbjct: 716 PVIKKMKK*YRNNYKNSMSSTVVIRSTISQSCDCQKEAGDNNKSRVITCPLIL 874
>gb|CX168823.1|CX168823 E07_69-80_09.ab1 leaf inoculated with Marssonia pathogen of Populus
deltoides Populus deltoides cDNA, mRNA sequence
Length = 656
Score = 211 bits (455), Expect(2) = 1e-085
Identities = 82/111 (73%), Positives = 89/111 (80%)
Frame = +2 / +3
Query: 161 YLRRIVKWQQRDIEYTFWQMVHLCTSPKVVYQHTKYHKQTKNQWARDDPAFVVILILFLV 340
YLRRI+KWQQ DIEYTFWQM++LCTSPKVVYQHTK+HKQTKNQWARDDPAFVVI L L
Sbjct: 159 YLRRIIKWQQMDIEYTFWQMLYLCTSPKVVYQHTKFHKQTKNQWARDDPAFVVISSLLLA 338
Query: 341 FATSAYCAAYGESASHAALTITSVVFLHFLFAGIVLATLCWFLTNSYLREE 493
A AYCAAY SA HA + SV+ HFL G LAT CWFLTN+YLREE
Sbjct: 339 VAALAYCAAYDHSAGHAVFVVISVLLFHFLITGAGLATCCWFLTNAYLREE 491
Score = 147 bits (316), Expect(2) = 5e-056
Identities = 66/105 (62%), Positives = 78/105 (74%)
Frame = -3 / -2
Query: 492 SSLK*EFVRNQQSVAKTIPANKKWRNTTEVIVRAACEALSPYAAQ*ADVANTRKRIRITT 313
SS +* VRNQQ VA+ P KK ++ TE+ + AC AL YAAQ*A A R+R+ ITT
Sbjct: 490 SSRR*ALVRNQQHVARPAPVIKK*KSNTEITTKTACPALWSYAAQ*AKAATARRRLEITT 311
Query: 312 NAGSSRAHWFFVCL*YLVC**TTFGEVQR*TICQNVYSISLCCHL 178
AGSSRAH F VCL* LVC**TTFGEV +*+ICQ VYS+S+CCHL
Sbjct: 310 KAGSSRAH*FLVCL*NLVC**TTFGEVHK*SICQKVYSMSICCHL 176
Score = 126 bits (270), Expect(2) = 1e-085
Identities = 49/56 (87%), Positives = 51/56 (91%)
Frame = +2 / +3
Query: 488 EEPNSHVVEQRVEWLYAFDVHCNSFFPAFVILYVVQYFLSPLLVAHGFFPALLSNL 655
E PNSHVVEQRVEWLYAFDVHCNSFFP FV+LYV+ YFLSPLLVAHGF P LLSNL
Sbjct: 489 EAPNSHVVEQRVEWLYAFDVHCNSFFPMFVMLYVIHYFLSPLLVAHGFIPVLLSNL 656
Score = 97.3 bits (206), Expect(2) = 1e-026
Identities = 44/103 (42%), Positives = 59/103 (57%)
Frame = +1 / +2
Query: 160 LPPPHRQVATKGY*IHILANGSSLYFTKSSLSAHQISQANKEPVGP**SCICCYSDSFPR 339
+PP + QVAT G+*IH LAN ++ T+S LSA+ +SQ N E +G ** C CCY
Sbjct: 158 IPPSNYQVATNGH*IHFLANALLMHLTESCLSAY*VSQTN*ESMGT**PCFCCYLQPPSG 337
Query: 340 VCNISLLCSIWRECLTCCSNNHFGCVSPFLVCWDSFGHTLLVP 468
+ LLC + +C TCC ++F F W G+ LLVP
Sbjct: 338 SRSFGLLCCV*PQCWTCCFCSYFCITFSFFNYWSWSGYMLLVP 466
Score = 93.6 bits (198), Expect(2) = 1e-024
Identities = 47/114 (41%), Positives = 70/114 (61%)
Frame = -2 / -1
Query: 490 LSQVRICKEPAKCGQNYPSKQEMEKHNRSDC*SSM*GTLSICCTVS*CCKHEEKNQNNNK 311
L +V I EP C Q S +M+K R++ +SM T+ I T+S C +++ +NNK
Sbjct: 488 LPKVSIS*EPTTCSQTSSSN*KMKK*YRNNYKNSMSSTVVIRSTISQSCDCQKEAGDNNK 309
Query: 310 CRIITGPLVLCLLVIFGVLIDYFW*STEMNHLPKCVFNIPLLPLDDAAEVKKKT 149
R+IT PL+L L V +LID F * + HLP+ VFN+ LLPLD++ EV +++
Sbjct: 308 SRVITCPLILSLFVKLSMLIDNFR*GA*VKHLPESVFNVHLLPLDNSTEVLRES 147
Score = 91.3 bits (193), Expect(2) = 5e-056
Identities = 39/56 (69%), Positives = 41/56 (73%)
Frame = -3 / -2
Query: 654 RLDNKAGKKPCATNRGDRKYCTTYRMTNAGKNELQ*TSNA*SHSTRCSTTWLLGSS 487
R D+ G KP AT RGDRK C T MTN GK ELQ TSNA SHSTRCSTTWL G+S
Sbjct: 655 RFDSNTGMKPWATRRGDRK*CIT*SMTNIGKKELQCTSNAYSHSTRCSTTWLFGAS 488
Score = 43.7 bits (89), Expect(2) = 1e-026
Identities = 18/42 (42%), Positives = 24/42 (57%)
Frame = +1 / +2
Query: 502 PCC*ATRGVALCI*RSL*LVLSCVCHPICGAVFSVASIGRAW 627
PCC*AT +A+C+* +L L VCH +C +F V W
Sbjct: 503 PCC*ATG*MAICV*CALQFFLPNVCHALCYTLFPVTPSSGPW 628
Score = 40.5 bits (82), Expect(2) = 1e-024
Identities = 16/36 (44%), Positives = 27/36 (75%)
Frame = -2 / -1
Query: 604 QKILHHI*DDKRRKERVTMNVKCIEPLHALLNNMAV 497
Q+I+++I DK +ER+ +++K I P + LLNNMA+
Sbjct: 605 QEIMYNIKHDKHWEERIAVHIKRI*PFNPLLNNMAI 498
>gb|CN518663.1|CN518663 GQ0102.B3_A24 GQ010 Populus trichocarpa x Populus deltoides cDNA
clone GQ0102_A24 5', mRNA sequence
Length = 611
Score = 242 bits (523), Expect = 3e-063
Identities = 93/113 (82%), Positives = 105/113 (92%)
Frame = +2 / +3
Query: 494 PNSHVVEQRVEWLYAFDVHCNSFFPAFVILYVVQYFLSPLLVAHGFFPALLSNLLFVVAI 673
PNSHVVEQRVEWLYAFDVHCNSFFP FV+LYV+ YFLSPLLVAHGF P LLSNLLF+VA
Sbjct: 3 PNSHVVEQRVEWLYAFDVHCNSFFPMFVMLYVIHYFLSPLLVAHGFIPVLLSNLLFMVAA 182
Query: 674 SYYHYLNFLGYDVLPFLDRTTFFLYPIGLVIILSPLMILIGFNPTRYFLSLYF 832
SYYHYLNFLGY VLPFL+RTTFFLYPIGL+I+LSP++IL GFNP+RY +++YF
Sbjct: 183 SYYHYLNFLGYYVLPFLERTTFFLYPIGLIIVLSPILILSGFNPSRYLMNVYF 341
Score = 159 bits (342), Expect = 3e-038
Identities = 77/116 (66%), Positives = 80/116 (68%)
Frame = -3 / -2
Query: 840 L*PKYKLRKYLVGLNPISIISGDRMMTRPIGYRKNVVLSRNGRTSYPRKFR****EIATT 661
L* KY KYL GL P+ I GDR M RPIG RKNVVLSR G T P+K R*** E AT
Sbjct: 349 L*LKYTFIKYLEGLKPLKIKIGDRTMIRPIG*RKNVVLSRKGNT**PKKLR***YEAATM 170
Query: 660 KSRLDNKAGKKPCATNRGDRKYCTTYRMTNAGKNELQ*TSNA*SHSTRCSTTWLLG 493
SR D+ G KP AT RGDRK C T MTN GK ELQ TSNA SHSTRCSTTWL G
Sbjct: 169 NSRFDSNTGMKPWATRRGDRK*CIT*SMTNIGKKELQCTSNAYSHSTRCSTTWLFG 2
>gb|BI068874.1|BI068874 C028P46U Populus strain T89 leaves Populus tremula x Populus
tremuloides cDNA, mRNA sequence
Length = 408
Score = 126 bits (269), Expect(2) = 6e-029
Identities = 48/63 (76%), Positives = 53/63 (84%)
Frame = +2 / +2
Query: 161 YLRRIVKWQQRDIEYTFWQMVHLCTSPKVVYQHTKYHKQTKNQWARDDPAFVVILILFLV 340
YLRRI+KWQQ DIEYTFWQM++LCTSPKV YQHTK+HKQTKNQWARDDPA I L L
Sbjct: 155 YLRRIIKWQQMDIEYTFWQMLYLCTSPKVXYQHTKFHKQTKNQWARDDPAXXGISSLLLA 334
Query: 341 FAT 349
A+
Sbjct: 335 VAS 343
Score = 22.1 bits (42), Expect(2) = 6e-029
Identities = 8/10 (80%), Positives = 8/10 (80%)
Frame = +2 / +3
Query: 353 AYCAAYGESA 382
AYCAAY SA
Sbjct: 348 AYCAAYDHSA 377
>gb|BP924384.1|BP924384 BP924384 full-length enriched poplar cDNA library Populus nigra
cDNA clone PnFL1-031_I06.f 5', mRNA sequence
Length = 366
Score = 118 bits (253), Expect = 6e-026
Identities = 42/46 (91%), Positives = 45/46 (97%)
Frame = +2 / +2
Query: 161 YLRRIVKWQQRDIEYTFWQMVHLCTSPKVVYQHTKYHKQTKNQWAR 298
YLRRI+KWQQ D+EYTFWQM+HLCTSPKVVYQHTKYHKQTKNQWAR
Sbjct: 227 YLRRIIKWQQMDVEYTFWQMLHLCTSPKVVYQHTKYHKQTKNQWAR 364
Database: Populus_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:49 PM
Number of letters in database: 203,408,664
Number of sequences in database: 369,679
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 623,321,619
Number of Sequences: 369679
Number of extensions: 9359523
Number of successful extensions: 49392
Number of sequences better than 1.0e-020: 7
length of database: 67,802,888
effective HSP length: 53
effective length of database: 48,209,901
effective search space used: 16777045548
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)