TBLASTX 2.2.10 [Oct-19-2004]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 2655972.2.1
         (666 letters)

Database: Populus_nucl_with_EST.fasta 
           369,679 sequences; 203,408,664 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|BU873630.1|BU873630  Q057F07 Populus flower cDNA library ...   160   8e-039

>gb|BU873630.1|BU873630 Q057F07 Populus flower cDNA library Populus trichocarpa cDNA 5
           prime, mRNA sequence
          Length = 633

 Score =  160 bits (344), Expect = 8e-039
 Identities = 62/93 (66%), Positives = 77/93 (82%)
 Frame = +2 / +1

                                                                       
Query: 2   KGLPGCS*TXSPVLFFFGCRNQENDFLYKDFWLTHAQDEGVLSSKRGGGFFVAFSRDQPQ 181
           + +   S + +P++ FFGC+NQENDFLYKDFWL+HAQ+ G LS  RGGGF+VAFSRDQPQ
Sbjct: 355 RAIQDMSGSAAPIMLFFGCQNQENDFLYKDFWLSHAQNGGPLSIARGGGFYVAFSRDQPQ 534

                                            
Query: 182 KVYVQPKIKEQSSRLWNLLLSDAVIYIAGSSTK 280
           KVYVQ K++EQS R+WNLL+  A IY+AGSSTK
Sbjct: 535 KVYVQHKMREQSQRVWNLLVEGASIYVAGSSTK 633




 Score =  109 bits (232), Expect = 2e-023
 Identities = 52/81 (64%), Positives = 58/81 (71%)
 Frame = -1 / -2

                                                                       
Query: 276 VEEPAM*ITASDSNKFHSLELCSFILGCT*TFCG*SREKATKKPPPLFEDNTPSSCA*VS 97
           V+EPA  I A  +NKF +L LCS IL CT*TFCG*S EKAT  PPPL  D+ P  CA* +
Sbjct: 629 VDEPATYIEAPSTNKFQTLWLCSRILCCT*TFCG*SLEKAT*NPPPLAMDSGPPFCA*DN 450

                                
Query: 96  QKSLYRKSFSWFLQPKKNRTG 34
           QKSLY+KSFS F QPK N  G
Sbjct: 449 QKSLYKKSFS*F*QPKNNIIG 387



  Database: Populus_nucl_with_EST.fasta
    Posted date:  Feb 7, 2006  2:49 PM
  Number of letters in database: 203,408,664
  Number of sequences in database:  369,679
  
Lambda     K      H
   0.318    0.134    0.401 


Matrix: BLOSUM62
Number of Hits to DB: 349,911,898
Number of Sequences: 369679
Number of extensions: 5135572
Number of successful extensions: 47393
Number of sequences better than 1.0e-020: 1
length of database: 67,802,888
effective HSP length: 51
effective length of database: 48,949,259
effective search space used: 8321374030
frameshift window, decay const: 40,  0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)