TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2594405.2.1
(637 letters)
Database: Populus_nucl_with_EST.fasta
369,679 sequences; 203,408,664 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CK087762.1|CK087762 A020P21.3pR Hybrid aspen plasmid lib... 270 4e-072
gb|AY935501.1| Populus tremula x Populus tremuloides putati... 269 2e-071
gb|BU895901.1|BU895901 X032G04 Populus wood cDNA library Po... 254 3e-067
gb|CF233438.1|CF233438 PtaJXO0023F11F1111 Poplar cDNA libra... 203 7e-053
gb|CV244122.1|CV244122 WS0253.B21_M19 PT-MB-N-A-15 Populus ... 185 3e-046
gb|CF235078.1|CF235078 PtaJXT0018F2F0212 Poplar cDNA librar... 152 2e-036
>gb|CK087762.1|CK087762 A020P21.3pR Hybrid aspen plasmid library Populus tremula x Populus
tremuloides cDNA clone A020P21 3', mRNA sequence
Length = 680
Score = 270 bits (585), Expect = 4e-072
Identities = 101/162 (62%), Positives = 135/162 (83%)
Frame = -2 / -2
Query: 630 GAPVGGIGVQGHISHPVGEIICDSLDKLAILGLPIWITELDVTAENEHIRADDLEVFLRE 451
GAPVGGIG+QGHI PVG ++C +LDKL ILGLPIW TELDV++ NE +R DDLEV LRE
Sbjct: 679 GAPVGGIGIQGHIDSPVGPVVCSALDKLGILGLPIWFTELDVSSVNECVRGDDLEVMLRE 500
Query: 450 AFAHPAVGGIILWGFWEMFMFREHAHLVDADGTINEAGRRYLALKQEWLTRMNGSVDHQG 271
A+AHPAV G++LWGFWE+FM R++AHLV+A+G +NEAG+RYLALK+EWL+R +G +D QG
Sbjct: 499 AYAHPAVDGVMLWGFWELFMSRDNAHLVNAEGELNEAGKRYLALKKEWLSRAHGHIDEQG 320
Query: 270 EFKFRGYHGSYTVEVNTPSGKVARSFVVDKDSPVQVIALNV* 145
+F FRG+HG+Y +E+ T S K+ ++FVVDK V+++++*
Sbjct: 319 QFAFRGFHGTYVLEIETVSKKMVKTFVVDKGDSPLVVSIDL* 194
Score = 131 bits (280), Expect = 5e-030
Identities = 72/150 (48%), Positives = 80/150 (53%)
Frame = +2 / +3
Query: 182 LSTTNDLATLPEGVFTSTVYEPWYPLNLNSPW*STLPFIRVSHSCLRARYLLPASLIVPS 361
LSTTN E V S P PLN N P S P R SHS LRARYLLPASL PS
Sbjct: 231 LSTTNVFTIFLETVSISKT*VP*KPLNANCPCSSICP*ARDSHSFLRARYLLPASLSSPS 410
Query: 362 ASTRWACSRNINISQNPQRMIPPTAGCXXXXXXXXXXXXXXMCSFSAVTSNSVIQIGRPS 541
A T+ A SR +N SQNPQ + P TAG S + TS+SV IGRP
Sbjct: 411 ALTKCALSRLMNNSQNPQSITPSTAG*AYASRSITSKSSPLTHSLTEDTSSSVNHIGRPR 590
Query: 542 MASLSRESHMISPTGWLI*PCTPIPPTGAP 631
+ +LSR PTG I P P+PPTGAP
Sbjct: 591 IPNLSRAEQTTGPTGLSICP*IPMPPTGAP 680
>gb|AY935501.1| Populus tremula x Populus tremuloides putative endo-1,4-beta-xylanase
mRNA, complete cds
Length = 3001
Score = 269 bits (581), Expect = 2e-071
Identities = 100/164 (60%), Positives = 136/164 (82%)
Frame = -2 / +1
Query: 636 DRGAPVGGIGVQGHISHPVGEIICDSLDKLAILGLPIWITELDVTAENEHIRADDLEVFL 457
++GAPVGGIG+QGHI PVG ++C +LDKL ILGLPIW TELDV++ NE +R DDLEV L
Sbjct: 2332 EQGAPVGGIGIQGHIDSPVGPVVCSALDKLGILGLPIWFTELDVSSVNECVRGDDLEVML 2511
Query: 456 REAFAHPAVGGIILWGFWEMFMFREHAHLVDADGTINEAGRRYLALKQEWLTRMNGSVDH 277
REA+AHPAV G++LWGFWE+FM R++AH V+A+G +NEAG+RYLALK+EWL+R +G +D
Sbjct: 2512 REAYAHPAVDGVMLWGFWELFMSRDNAHPVNAEGELNEAGKRYLALKKEWLSRAHGHIDE 2691
Query: 276 QGEFKFRGYHGSYTVEVNTPSGKVARSFVVDKDSPVQVIALNV* 145
QG+F FRG+HG+Y +E+ T S K+ ++FVVDK V+++++*
Sbjct: 2692 QGQFAFRGFHGTYVLEIETVSKKMVKTFVVDKGDSPLVVSIDL* 2823
Score = 132 bits (282), Expect = 3e-030
Identities = 74/152 (48%), Positives = 80/152 (52%)
Frame = +2 / -3
Query: 182 LSTTNDLATLPEGVFTSTVYEPWYPLNLNSPW*STLPFIRVSHSCLRARYLLPASLIVPS 361
LSTTN E V S P PLN N P S P R SHS LRARYLLPASL PS
Sbjct: 2786 LSTTNVFTIFLETVSISKT*VP*KPLNANCPCSSICP*ARDSHSFLRARYLLPASLSSPS 2607
Query: 362 ASTRWACSRNINISQNPQRMIPPTAGCXXXXXXXXXXXXXXMCSFSAVTSNSVIQIGRPS 541
A T A SR IN SQNPQ + P TAG S + TS+SV IGRP
Sbjct: 2606 ALTGCALSRLINNSQNPQSITPSTAG*AYASRSITSKSSPLTHSLTEDTSSSVNHIGRPR 2427
Query: 542 MASLSRESHMISPTGWLI*PCTPIPPTGAPLS 637
+ +LSR PTG I P P+PPTGAP S
Sbjct: 2426 IPNLSRAEQTTGPTGLSICP*IPMPPTGAPCS 2331
>gb|BU895901.1|BU895901 X032G04 Populus wood cDNA library Populus tremula x Populus
tremuloides cDNA 5 prime, mRNA sequence
Length = 621
Score = 254 bits (550), Expect = 3e-067
Identities = 93/147 (63%), Positives = 124/147 (84%)
Frame = -2 / +1
Query: 636 DRGAPVGGIGVQGHISHPVGEIICDSLDKLAILGLPIWITELDVTAENEHIRADDLEVFL 457
++GAPVGGIG+QGHI PVG ++C +LDKL ILGLPIW TELDV++ NE +R DD EV L
Sbjct: 181 EQGAPVGGIGIQGHIDSPVGPVVCSALDKLGILGLPIWFTELDVSSVNECVRGDDSEVML 360
Query: 456 REAFAHPAVGGIILWGFWEMFMFREHAHLVDADGTINEAGRRYLALKQEWLTRMNGSVDH 277
REA+AHPAV G++LWGFWE+FM R++AHLV+A+G +NEAG+RYLALK+EWL+R +G +D
Sbjct: 361 REAYAHPAVDGVMLWGFWELFMSRDNAHLVNAEGELNEAGKRYLALKKEWLSRAHGHIDE 540
Query: 276 QGEFKFRGYHGSYTVEVNTPSGKVARS 196
QG+F FRG+HG+Y +E+ T S K+ ++
Sbjct: 541 QGQFAFRGFHGTYVLEIETVSKKMVKT 621
Score = 126 bits (269), Expect = 2e-028
Identities = 69/141 (48%), Positives = 76/141 (53%)
Frame = +2 / -2
Query: 215 EGVFTSTVYEPWYPLNLNSPW*STLPFIRVSHSCLRARYLLPASLIVPSASTRWACSRNI 394
E V S P PLN N P S P R SHS LRARYLLPASL PSA TR A SR +
Sbjct: 602 ETVSISKT*VP*KPLNANCPCSSICP*ARDSHSFLRARYLLPASLSSPSALTRCALSRLM 423
Query: 395 NISQNPQRMIPPTAGCXXXXXXXXXXXXXXMCSFSAVTSNSVIQIGRPSMASLSRESHMI 574
N SQNPQ + P TAG S + TS+SV IGRP + +LSR
Sbjct: 422 NNSQNPQSITPSTAG*AYASRSITSESSPLTHSLTEDTSSSVNHIGRPRIPNLSRAEQTT 243
Query: 575 SPTGWLI*PCTPIPPTGAPLS 637
PTG I P P+PPTGAP S
Sbjct: 242 GPTGLSICP*IPMPPTGAPCS 180
>gb|CF233438.1|CF233438 PtaJXO0023F11F1111 Poplar cDNA library from young opposite xylem
Populus alba x Populus tremula cDNA 5', mRNA sequence
Length = 519
Score = 203 bits (437), Expect(2) = 7e-053
Identities = 79/139 (56%), Positives = 107/139 (76%)
Frame = -2 / +2
Query: 561 SLDKLAILGLPIWITELDVTAENEHIRADDLEVFLREAFAHPAVGGIILWGFWEMFMFRE 382
+LDKL ILGLPIW TELDV++ NE +R DDLEV LREA+AHP GI+L GFWE+FM R+
Sbjct: 71 ALDKLGILGLPIWFTELDVSSVNECVRGDDLEVMLREAYAHPXXDGIMLXGFWELFMSRD 250
Query: 381 HAHLVDADGTINEAGRRYLALKQEWLTRMNGSVDHQGEFKFRGYHGSYTVEVNTPSGKVA 202
+AH V+A +NEAG+RYL+LK+EWL R +G +D QG+F FRG+HG+Y +E+ T S K+
Sbjct: 251 NAHXVNAXRXLNEAGKRYLSLKKEWLXRAHGHIDEQGQFAFRGFHGTYVLEIETVSKKMT 430
Query: 201 RSFVVDKDSPVQVIALNV* 145
++FV DK V ++++*
Sbjct: 431 KTFVXDKGGSPLVXSIDL* 487
Score = 24.4 bits (47), Expect(2) = 7e-053
Identities = 9/12 (75%), Positives = 10/12 (83%)
Frame = -2 / +1
Query: 627 APVGGIGVQGHI 592
APVGGIG+Q I
Sbjct: 4 APVGGIGIQXFI 39
>gb|CV244122.1|CV244122 WS0253.B21_M19 PT-MB-N-A-15 Populus trichocarpa cDNA clone
WS0253_M19 3', mRNA sequence
Length = 807
Score = 185 bits (398), Expect = 3e-046
Identities = 72/124 (58%), Positives = 99/124 (79%)
Frame = -2 / -1
Query: 516 ELDVTAENEHIRADDLEVFLREAFAHPAVGGIILWGFWEMFMFREHAHLVDADGTINEAG 337
ELDV+ NE +RADDLEV LREAFAHPAV G+ILWGFWE++M R++AHLV+ADG IN AG
Sbjct: 807 ELDVSCPNEFVRADDLEVVLREAFAHPAVEGVILWGFWELYMSRKNAHLVNADGKINAAG 628
Query: 336 RRYLALKQEWLTRMNGSVDHQGEFKFRGYHGSYTVEVNTPSGKVARSFVVDKDSPVQVIA 157
+R+L+LK+EWL+ +G +D GEF+FRG+HG+Y VE + + KV +FVVD+ V++
Sbjct: 627 KRFLSLKKEWLSHASGHIDELGEFRFRGFHGTYNVEFVSTTEKVNMTFVVDQGESPLVVS 448
Query: 156 LNV* 145
+++*
Sbjct: 447 IDL* 436
>gb|CF235078.1|CF235078 PtaJXT0018F2F0212 Poplar cDNA library from young tension xylem
Populus alba x Populus tremula cDNA 5', mRNA sequence
Length = 662
Score = 152 bits (327), Expect = 2e-036
Identities = 55/78 (70%), Positives = 68/78 (87%)
Frame = -2 / +1
Query: 636 DRGAPVGGIGVQGHISHPVGEIICDSLDKLAILGLPIWITELDVTAENEHIRADDLEVFL 457
++GAPVGGIG+QGHI PVG ++C +LDKL ILGLPIW TELDV++ NE++R DDLEV L
Sbjct: 379 EQGAPVGGIGIQGHIDSPVGPVVCSALDKLGILGLPIWFTELDVSSVNEYVRGDDLEVML 558
Query: 456 REAFAHPAVGGIILWGFW 403
REA+AHPAV G++LWGFW
Sbjct: 559 REAYAHPAVDGVMLWGFW 612
Database: Populus_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:49 PM
Number of letters in database: 203,408,664
Number of sequences in database: 369,679
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 371,297,357
Number of Sequences: 369679
Number of extensions: 6175137
Number of successful extensions: 166752
Number of sequences better than 1.0e-020: 8
length of database: 67,802,888
effective HSP length: 51
effective length of database: 48,949,259
effective search space used: 7831881440
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)