TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2493961.2.1
(733 letters)
Database: Populus_nucl_with_EST.fasta
369,679 sequences; 203,408,664 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|DT513199.1|DT513199 WS02421.B21_G10 PTxD-ICC-N-A-14 Popu... 102 3e-033
gb|DT488399.1|DT488399 WS02536.B21_F06 PT-MB-N-A-15 Populus... 103 7e-033
gb|CV229369.1|CV229369 WS01913.B21_B10 PT-DX-N-A-10 Populus... 70 6e-025
gb|CK318227.1|CK318227 B9P06h06 Populus stem seasonal libra... 70 6e-025
gb|DN491794.1|DN491794 V020F03.3pR Populus male catkins cDN... 70 6e-025
gb|CK087291.1|CK087291 A002P26.3pR Hybrid aspen plasmid lib... 69 1e-024
gb|CX173875.1|CX173875 D12_69-74_08.ab1 leaf inoculated wit... 102 3e-021
gb|CK092568.1|CK092568 G087P77.3pR Populus tension wood cDN... 48 8e-021
>gb|DT513199.1|DT513199 WS02421.B21_G10 PTxD-ICC-N-A-14 Populus trichocarpa x Populus
deltoides cDNA clone WS02421_G10 3', mRNA sequence
Length = 906
Score = 102 bits (217), Expect(2) = 3e-033
Identities = 38/59 (64%), Positives = 44/59 (74%)
Frame = -2 / -2
Query: 390 GVHGWRRPFVTHFTGCQPCSGQRNEHYSGDSCDQGMRRALNFADDQVLRAYGFRHASSL 214
G WRRPF+THFTGCQPCSG N+ Y G++C GM +ALNFAD+QVLR YGF H L
Sbjct: 629 GKGSWRRPFITHFTGCQPCSGDHNQIYHGETCWNGMVKALNFADNQVLRKYGFVHPDLL 453
Score = 59.7 bits (124), Expect(2) = 3e-033
Identities = 23/62 (37%), Positives = 36/62 (58%)
Frame = -2 / -2
Query: 672 QDKVSRVDDQSALVYMLLQEGSRWRDKVFLESGYYFEGYWMEIVGRLANMTERYEAMERR 493
+DK+ D + + +L + DK++LE YYFEGYW +IV N+TE+Y +E+
Sbjct: 896 KDKLFPESDDQSGLIYMLYQDKGLMDKIYLEGEYYFEGYWADIVPTYDNITEKYTELEKE 717
Query: 492 PG 487
G
Sbjct: 716 DG 711
>gb|DT488399.1|DT488399 WS02536.B21_F06 PT-MB-N-A-15 Populus trichocarpa cDNA clone
WS02536_F06 3', mRNA sequence
Length = 842
Score = 103 bits (220), Expect(2) = 7e-033
Identities = 38/62 (61%), Positives = 45/62 (72%)
Frame = -2 / -2
Query: 390 GVHGWRRPFVTHFTGCQPCSGQRNEHYSGDSCDQGMRRALNFADDQVLRAYGFRHASSLS 211
G WRRPF+THFTGCQPCSG N+ Y G++C GM +ALNFAD+QVLR YGF H L
Sbjct: 526 GKGSWRRPFITHFTGCQPCSGDHNQMYEGETCWNGMVKALNFADNQVLRKYGFVHPDLLD 347
Query: 210 DD 205
+
Sbjct: 346 SN 341
Score = 57.0 bits (118), Expect(2) = 7e-033
Identities = 22/62 (35%), Positives = 36/62 (58%)
Frame = -2 / -2
Query: 672 QDKVSRVDDQSALVYMLLQEGSRWRDKVFLESGYYFEGYWMEIVGRLANMTERYEAMERR 493
+DK+ D + + LL + +K++LE YYFEGYW +I+ N+TE+Y +E+
Sbjct: 793 KDKLFPESDDQSGLTYLLYKDKSLTEKIYLEGEYYFEGYWADILPTYDNITEKYTELEKE 614
Query: 492 PG 487
G
Sbjct: 613 DG 608
>gb|CV229369.1|CV229369 WS01913.B21_B10 PT-DX-N-A-10 Populus trichocarpa cDNA clone
WS01913_B10 3', mRNA sequence
Length = 817
Score = 69.8 bits (146), Expect(3) = 6e-025
Identities = 24/47 (51%), Positives = 34/47 (72%)
Frame = -2 / -1
Query: 651 DDQSALVYMLLQEGSRWRDKVFLESGYYFEGYWMEIVGRLANMTERY 511
DDQSAL+Y+LL + ++W DKV++E+ YY GYW +V R M E+Y
Sbjct: 682 DDQSALIYLLLSQKAQWMDKVYIENQYYLHGYWAGLVDRYEEMMEKY 542
Score = 46.4 bits (95), Expect(3) = 6e-025
Identities = 18/38 (47%), Positives = 24/38 (63%)
Frame = -2 / -1
Query: 312 YSGDSCDQGMRRALNFADDQVLRAYGFRHASSLSDDVQ 199
Y + C + M RA NFAD+QVL+ YGF H LS ++
Sbjct: 463 YPVEQCMRSMERAFNFADNQVLKLYGFGHRGLLSPKIK 350
Score = 35.4 bits (71), Expect(3) = 6e-025
Identities = 12/19 (63%), Positives = 14/19 (73%)
Frame = -2 / -1
Query: 390 GVHGWRRPFVTHFTGCQPC 334
G+ R PFVTHF GC+PC
Sbjct: 535 GLGDERWPFVTHFVGCKPC 479
>gb|CK318227.1|CK318227 B9P06h06 Populus stem seasonal library Populus deltoides cDNA, mRNA
sequence
Length = 760
Score = 69.8 bits (146), Expect(3) = 6e-025
Identities = 24/47 (51%), Positives = 34/47 (72%)
Frame = -2 / +3
Query: 651 DDQSALVYMLLQEGSRWRDKVFLESGYYFEGYWMEIVGRLANMTERY 511
DDQSAL+Y+LL + ++W DKV++E+ YY GYW +V R M E+Y
Sbjct: 321 DDQSALIYLLLSQKAQWMDKVYIENQYYLHGYWAGLVDRYEEMMEKY 461
Score = 46.4 bits (95), Expect(3) = 6e-025
Identities = 18/38 (47%), Positives = 24/38 (63%)
Frame = -2 / +3
Query: 312 YSGDSCDQGMRRALNFADDQVLRAYGFRHASSLSDDVQ 199
Y + C + M RA NFAD+QVL+ YGF H LS ++
Sbjct: 540 YPVEQCMRSMERAFNFADNQVLKLYGFGHRGLLSPKIK 653
Score = 35.4 bits (71), Expect(3) = 6e-025
Identities = 12/19 (63%), Positives = 14/19 (73%)
Frame = -2 / +3
Query: 390 GVHGWRRPFVTHFTGCQPC 334
G+ R PFVTHF GC+PC
Sbjct: 468 GLGDERWPFVTHFVGCKPC 524
>gb|DN491794.1|DN491794 V020F03.3pR Populus male catkins cDNA library Populus trichocarpa
cDNA clone V020F03 3', mRNA sequence
Length = 685
Score = 69.8 bits (146), Expect(3) = 6e-025
Identities = 24/47 (51%), Positives = 34/47 (72%)
Frame = -2 / -1
Query: 651 DDQSALVYMLLQEGSRWRDKVFLESGYYFEGYWMEIVGRLANMTERY 511
DDQSAL+Y+LL + ++W DKV++E+ YY GYW +V R M E+Y
Sbjct: 487 DDQSALIYLLLSQKAQWMDKVYIENQYYLHGYWAGLVDRYEQMMEKY 347
Score = 46.4 bits (95), Expect(3) = 6e-025
Identities = 18/38 (47%), Positives = 24/38 (63%)
Frame = -2 / -1
Query: 312 YSGDSCDQGMRRALNFADDQVLRAYGFRHASSLSDDVQ 199
Y + C + M RA NFAD+QVL+ YGF H LS ++
Sbjct: 268 YPVEQCMRSMERAFNFADNQVLKLYGFGHRGLLSPKIK 155
Score = 35.4 bits (71), Expect(3) = 6e-025
Identities = 12/19 (63%), Positives = 14/19 (73%)
Frame = -2 / -1
Query: 390 GVHGWRRPFVTHFTGCQPC 334
G+ R PFVTHF GC+PC
Sbjct: 340 GLGDERWPFVTHFVGCKPC 284
>gb|CK087291.1|CK087291 A002P26.3pR Hybrid aspen plasmid library Populus tremula x Populus
tremuloides cDNA clone A002P26 3', mRNA sequence
Length = 805
Score = 69.3 bits (145), Expect(3) = 1e-024
Identities = 24/47 (51%), Positives = 33/47 (70%)
Frame = -2 / -1
Query: 651 DDQSALVYMLLQEGSRWRDKVFLESGYYFEGYWMEIVGRLANMTERY 511
DDQSAL+Y+LL + +W DKV++E+ YY GYW +V R M E+Y
Sbjct: 757 DDQSALIYLLLSQKDQWMDKVYIENQYYLHGYWAGLVDRYEEMMEKY 617
Score = 46.0 bits (94), Expect(3) = 1e-024
Identities = 18/38 (47%), Positives = 24/38 (63%)
Frame = -2 / -1
Query: 312 YSGDSCDQGMRRALNFADDQVLRAYGFRHASSLSDDVQ 199
Y + C + M RA NFAD+QVL+ YGF H LS ++
Sbjct: 538 YPVERCMRSMERAFNFADNQVLKLYGFGHRGLLSPKIK 425
Score = 35.4 bits (71), Expect(3) = 1e-024
Identities = 12/19 (63%), Positives = 14/19 (73%)
Frame = -2 / -1
Query: 390 GVHGWRRPFVTHFTGCQPC 334
G+ R PFVTHF GC+PC
Sbjct: 610 GLGDERWPFVTHFVGCKPC 554
>gb|CX173875.1|CX173875 D12_69-74_08.ab1 leaf inoculated with Marssonia pathogen of Populus
deltoides Populus deltoides cDNA, mRNA sequence
Length = 675
Score = 102 bits (217), Expect = 3e-021
Identities = 38/59 (64%), Positives = 44/59 (74%)
Frame = -2 / +3
Query: 390 GVHGWRRPFVTHFTGCQPCSGQRNEHYSGDSCDQGMRRALNFADDQVLRAYGFRHASSL 214
G WRRPF+THFTGCQPCSG N+ Y G++C GM +ALNFAD+QVLR YGF H L
Sbjct: 129 GKGSWRRPFITHFTGCQPCSGDHNQIYHGETCWNGMVKALNFADNQVLRKYGFVHPDLL 305
>gb|CK092568.1|CK092568 G087P77.3pR Populus tension wood cDNA library Populus tremula x
Populus tremuloides cDNA clone G087P77 3', mRNA sequence
Length = 747
Score = 47.8 bits (98), Expect(4) = 8e-021
Identities = 15/39 (38%), Positives = 24/39 (61%)
Frame = -2 / -2
Query: 627 MLLQEGSRWRDKVFLESGYYFEGYWMEIVGRLANMTERY 511
++ + +W DKV++E+ YY GYW +V R M E+Y
Sbjct: 722 LVASQKDQWMDKVYIENQYYLHGYWAGLVDRYEEMIEKY 606
Score = 45.5 bits (93), Expect(4) = 8e-021
Identities = 18/38 (47%), Positives = 24/38 (63%)
Frame = -2 / -2
Query: 312 YSGDSCDQGMRRALNFADDQVLRAYGFRHASSLSDDVQ 199
Y + C + M RA NFAD+QVL+ YGF H LS ++
Sbjct: 527 YPVERCLRSMERAFNFADNQVLKLYGFGHRGLLSPKIK 414
Score = 35.4 bits (71), Expect(4) = 8e-021
Identities = 12/19 (63%), Positives = 14/19 (73%)
Frame = -2 / -2
Query: 390 GVHGWRRPFVTHFTGCQPC 334
G+ R PFVTHF GC+PC
Sbjct: 599 GLGDERWPFVTHFVGCKPC 543
Score = 25.8 bits (50), Expect(4) = 8e-021
Identities = 9/12 (75%), Positives = 12/12 (100%)
Frame = -2 / -3
Query: 651 DDQSALVYMLLQ 616
DDQSAL+Y+LL+
Sbjct: 745 DDQSALIYLLLR 710
Database: Populus_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:49 PM
Number of letters in database: 203,408,664
Number of sequences in database: 369,679
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 284,237,334
Number of Sequences: 369679
Number of extensions: 3474840
Number of successful extensions: 36040
Number of sequences better than 1.0e-020: 8
length of database: 67,802,888
effective HSP length: 52
effective length of database: 48,579,580
effective search space used: 9278699780
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)