TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2493528.2.1
(627 letters)
Database: Populus_nucl_with_EST.fasta
369,679 sequences; 203,408,664 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CF234505.1|CF234505 Ptajxojxo3F12F1212 Poplar cDNA libra... 281 3e-075
gb|DT517491.1|DT517491 WS02434.B21_H07 PTxD-ICC-N-A-14 Popu... 241 4e-069
gb|CV258030.1|CV258030 WS0248.B21_F10 PTxD-ICC-N-A-14 Popul... 157 7e-038
>gb|CF234505.1|CF234505 Ptajxojxo3F12F1212 Poplar cDNA library from young opposite xylem
Populus alba x Populus tremula cDNA 5', mRNA sequence
Length = 754
Score = 281 bits (608), Expect = 3e-075
Identities = 118/172 (68%), Positives = 144/172 (83%)
Frame = -2 / +1
Query: 599 EDRSLLLEKEYRNNILDDLLEVKSFLTQRLGEMRNADTSSLQHQVQAVSPFVLQQHAPDS 420
E RS LL+ EYRN ILDDL E+K+FL+QRL E+ N +T LQHQVQ+V+P VLQQ+ PD+
Sbjct: 91 EGRSQLLDTEYRNKILDDLYEIKAFLSQRLAELSNEETXGLQHQVQSVAPLVLQQYTPDA 270
Query: 419 LENMLVEISSAISLLTNQKTLDLIMILNSKRFLDRLVSSLEEKKHHEVKLREGLGDLSVK 240
++ M +S AISLLTN KT DLIM+LNSKR+LDRLVS+LE KKHHE+KL+EGL D++ K
Sbjct: 271 IDTMSSNVSLAISLLTNGKTRDLIMLLNSKRYLDRLVSTLEAKKHHELKLKEGLKDVAAK 450
Query: 239 RMELQNALSSSWPKQEAAITKTRELKKLCESTLSSVFDGRPVYIIGEINTLL 84
RMEL N++SSSWPKQEAA++KTRELKKLCE TLSS+FDGRPV IIG I L+
Sbjct: 451 RMELHNSISSSWPKQEAALSKTRELKKLCERTLSSMFDGRPVNIIGXIXALI 606
Score = 137 bits (294), Expect = 6e-032
Identities = 78/173 (45%), Positives = 89/173 (51%)
Frame = +1 / -3
Query: 88 KVLISPIIYTGLPSKTXXXXXXXXXXXXXXXXIAASCFGHDDDSAFCSSIRLTDKSPKPX 267
+ I PII TGLPS AASCFGHD+D SS+RL S P
Sbjct: 602 RAXIXPIILTGLPSNMDESVLSQSFFSSLVLERAASCFGHDEDIEL*SSMRLAATSFNPS 423
Query: 268 XXXXXXXXXXXKLETNLSRNLLELRIIIRSRVF*LVSNEIADEISTSMFSKLSGACCWRT 447
+ T+LSR LLELR II+S VF VS E+A E + S SG C T
Sbjct: 422 FNFNS*CFFASNVLTSLSRYLLELRSIIKSLVFPFVSKEMAKETLDDIVSIASGVYCCST 243
Query: 448 KGETACT*CCRDDVSAFLISPKR*VKNDFTSSKSSRILFLYSFSSNKLLSSAI 606
KG T T*CC VS+ L S *+KN F S KSSRILF YS S + LL S I
Sbjct: 242 KGATDWT*CCXPXVSSLLNSANL*LKNAFISYKSSRILFRYSVSKSWLLPSFI 84
Score = 104 bits (221), Expect = 7e-022
Identities = 65/168 (38%), Positives = 95/168 (56%)
Frame = +3 / -2
Query: 96 DLSNYIHWSSIKNRRQCRLTQXXXXXXXXXXXXXXWP***QCVLQLHTLNR*IT*TFP*F 275
DLS+YI+ SSIK+ R+C LT+ WP** +++ H + I * *F
Sbjct: 594 DLSDYINRSSIKHGRECPLTKFLQLPGFRKSCFLLWP**RYRIVKFHAFSSHIF*PLF*F 415
Query: 276 YFVMLLLF*A*N*SIQKPFRVKDHYKVKSLLIS*Q*DC*RDLYKHVLQTVRSMLLENKR* 455
+MLL F +*S+Q FRVK H +V I Q + R++ H + ++RS+LL+++
Sbjct: 414 QLMMLLCFQCTH*SVQISFRVKKHNQVSCFPICQQRNGQRNIR*HCINSIRSILLQHQGS 235
Query: 456 DSLHLML*R*CVCIPHLP*ALSQK*FYLE*IIQNIIPIFFLQQQTSIF 599
+ L LML + I L +L+QK FYL *II+N IPIF +Q+ F
Sbjct: 234 NRLDLMLQXXGLFITQLCQSLTQKCFYLV*IIKNFIPIFCIQKLAPAF 91
>gb|DT517491.1|DT517491 WS02434.B21_H07 PTxD-ICC-N-A-14 Populus trichocarpa x Populus
deltoides cDNA clone WS02434_H07 3', mRNA sequence
Length = 825
Score = 241 bits (520), Expect(2) = 4e-069
Identities = 102/154 (66%), Positives = 125/154 (81%)
Frame = -2 / -1
Query: 599 EDRSLLLEKEYRNNILDDLLEVKSFLTQRLGEMRNADTSSLQHQVQAVSPFVLQQHAPDS 420
E RS LL+ EYRN ILDDL E+K+FL+QRL E+ N +T SLQHQVQ+V+P VLQQ+ PD+
Sbjct: 618 EGRSQLLDTEYRNKILDDLYEIKAFLSQRLAELSNEETMSLQHQVQSVAPLVLQQYTPDA 439
Query: 419 LENMLVEISSAISLLTNQKTLDLIMILNSKRFLDRLVSSLEEKKHHEVKLREGLGDLSVK 240
++ M +S AISL+TN KT DLIM+LN KR+LDRL S+LE KKHHE+KL+EGL D++ K
Sbjct: 438 IDTMSSNVSLAISLMTNGKTRDLIMLLNYKRYLDRLASTLEAKKHHELKLKEGLKDVAGK 259
Query: 239 RMELQNALSSSWPKQEAAITKTRELKKLCESTLS 138
MEL N + SSWPKQEAA+ KTRELKKLCE TLS
Sbjct: 258 LMELHNYIYSSWPKQEAALAKTRELKKLCERTLS 157
Score = 107 bits (228), Expect(2) = 1e-026
Identities = 64/140 (45%), Positives = 75/140 (53%)
Frame = +1 / +2
Query: 187 AASCFGHDDDSAFCSSIRLTDKSPKPXXXXXXXXXXXXKLETNLSRNLLELRIIIRSRVF 366
AASCFGHD+ SS+ L S P + +LSR LL LR II+S VF
Sbjct: 206 AASCFGHDEYI*L*SSMSLPATSFNPSFNFNS*CFFASNVLASLSRYLL*LRSIIKSLVF 385
Query: 367 *LVSNEIADEISTSMFSKLSGACCWRTKGETACT*CCRDDVSAFLISPKR*VKNDFTSSK 546
V E+A E + S SG C TKG T T*CC+D VS+ L S +KN F S K
Sbjct: 386 PFVIKEMAKETLDDIVSIASGVYCCSTKGATDWT*CCKDMVSSLLNSANLWLKNAFISYK 565
Query: 547 SSRILFLYSFSSNKLLSSAI 606
SSRILF YS S + LL S I
Sbjct: 566 SSRILFRYSVSKSWLLPSFI 625
Score = 40.5 bits (82), Expect(2) = 4e-069
Identities = 16/22 (72%), Positives = 19/22 (86%)
Frame = -2 / -2
Query: 137 SVFDGRPVYIIGEINTLLSSSV 72
++FDGRPV IIGEIN LLSS +
Sbjct: 155 AMFDGRPVNIIGEINALLSSGI 90
Score = 32.2 bits (64), Expect(2) = 1e-026
Identities = 15/22 (68%), Positives = 19/22 (86%)
Frame = +3 / +3
Query: 69 TNT*AQ*SIDLSNYIHWSSIKN 134
TN+ AQ* IDLS+YI+ SSIK+
Sbjct: 87 TNSTAQ*GIDLSDYINRSSIKH 152
>gb|CV258030.1|CV258030 WS0248.B21_F10 PTxD-ICC-N-A-14 Populus trichocarpa x Populus
deltoides cDNA clone WS0248_F10 3', mRNA sequence
Length = 393
Score = 157 bits (337), Expect = 7e-038
Identities = 65/84 (77%), Positives = 75/84 (89%)
Frame = -2 / -3
Query: 323 LDRLVSSLEEKKHHEVKLREGLGDLSVKRMELQNALSSSWPKQEAAITKTRELKKLCEST 144
LDRLVS+LE KKHHE+KL+EGL D++ KRMEL N++SSSWPKQEAA+ KTRELKKLCE T
Sbjct: 391 LDRLVSTLEAKKHHELKLKEGLKDVAAKRMELHNSISSSWPKQEAALAKTRELKKLCERT 212
Query: 143 LSSVFDGRPVYIIGEINTLLSSSV 72
LSS+FDGRPV IIGEIN LLSS +
Sbjct: 211 LSSMFDGRPVNIIGEINALLSSGI 140
Database: Populus_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:49 PM
Number of letters in database: 203,408,664
Number of sequences in database: 369,679
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 275,492,029
Number of Sequences: 369679
Number of extensions: 3288617
Number of successful extensions: 37169
Number of sequences better than 1.0e-020: 4
length of database: 67,802,888
effective HSP length: 51
effective length of database: 48,949,259
effective search space used: 7685033663
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)