TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2455878.2.1
(1327 letters)
Database: Populus_nucl_with_EST.fasta
369,679 sequences; 203,408,664 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|DT503437.1|DT503437 WS0136.BR_J20 PTxD-IL-FL-A-4 Populus... 85 4e-028
gb|DT503659.1|DT503659 WS0137.BR_G07 PTxD-IL-FL-A-4 Populus... 85 5e-028
gb|DV465820.1|DV465820 MTUNUL1.P43.E12 NUL Populus fremonti... 85 7e-025
gb|CV280707.1|CV280707 WS0137.B21_G07 PTxD-IL-FL-A-4 Populu... 60 2e-022
gb|CV280517.1|CV280517 WS0136.B21_J20 PTxD-IL-FL-A-4 Populu... 60 2e-022
gb|CK096481.1|CK096481 UB15CPH11.3pR Populus active cambium... 61 2e-022
gb|DT504273.1|DT504273 WS01312.B21_L07 PTxD-IL-FL-A-4 Popul... 60 2e-022
gb|DT476355.1|DT476355 WS01229.B21_E11 PT-GT-FL-A-3 Populus... 60 6e-022
gb|CV235235.1|CV235235 WS01218.B21_E23 PT-GT-FL-A-3 Populus... 60 6e-022
gb|DN488662.1|DN488662 S021B06.3pR Populus imbibed seed cDN... 71 8e-021
>gb|DT503437.1|DT503437 WS0136.BR_J20 PTxD-IL-FL-A-4 Populus trichocarpa x Populus
deltoides cDNA clone WS0136_J20 5', mRNA sequence
Length = 864
Score = 85.4 bits (180), Expect(3) = 4e-028
Identities = 36/75 (48%), Positives = 50/75 (66%)
Frame = +2 / +3
Query: 68 AELLQAQADLWRHSLYYLTSMALKCAVELHIPTAIHNLGGSATLPDLVAALSLPAAKLPF 247
+ELLQAQ +W H Y++ SM LKCAV+L IP I G TL +LV+AL + +K +
Sbjct: 42 SELLQAQTHVWNHIFYFINSMTLKCAVQLGIPDVIQKHGKPMTLSELVSALPIHPSKAQY 221
Query: 248 LGRVMRLLVTSGVFA 292
+ R+MR+LV SG F+
Sbjct: 222 VHRLMRILVHSGFFS 266
Score = 54.2 bits (112), Expect(3) = 4e-028
Identities = 19/32 (59%), Positives = 28/32 (87%)
Frame = +2 / +3
Query: 785 AQAVMLKLVLHFWSDDDCVKILELCRKAIPSR 880
A+A++LK +LH WSD+DCVKIL+ C++AI S+
Sbjct: 765 AEAILLKWILHNWSDEDCVKILKRCKQAIMSK 860
Score = 29.5 bits (58), Expect(3) = 4e-028
Identities = 11/19 (57%), Positives = 14/19 (73%)
Frame = +2 / +3
Query: 683 AAAIVKAFPDIKCTVLNLP 739
A I +AFP + CTVL+LP
Sbjct: 633 AKGITEAFPHMDCTVLDLP 689
>gb|DT503659.1|DT503659 WS0137.BR_G07 PTxD-IL-FL-A-4 Populus trichocarpa x Populus
deltoides cDNA clone WS0137_G07 5', mRNA sequence
Length = 905
Score = 85.4 bits (180), Expect(3) = 5e-028
Identities = 36/75 (48%), Positives = 50/75 (66%)
Frame = +2 / +3
Query: 68 AELLQAQADLWRHSLYYLTSMALKCAVELHIPTAIHNLGGSATLPDLVAALSLPAAKLPF 247
+ELLQAQ +W H Y++ SM LKCAV+L IP I G TL +LV+AL + +K +
Sbjct: 45 SELLQAQTHVWNHIFYFINSMTLKCAVQLGIPDVIQKHGKPMTLSELVSALPIHPSKAQY 224
Query: 248 LGRVMRLLVTSGVFA 292
+ R+MR+LV SG F+
Sbjct: 225 VHRLMRILVHSGFFS 269
Score = 54.2 bits (112), Expect(3) = 5e-028
Identities = 19/32 (59%), Positives = 28/32 (87%)
Frame = +2 / +3
Query: 785 AQAVMLKLVLHFWSDDDCVKILELCRKAIPSR 880
A+A++LK +LH WSD+DCVKIL+ C++AI S+
Sbjct: 768 AEAILLKWILHNWSDEDCVKILKRCKQAIMSK 863
Score = 29.5 bits (58), Expect(3) = 5e-028
Identities = 11/19 (57%), Positives = 14/19 (73%)
Frame = +2 / +3
Query: 683 AAAIVKAFPDIKCTVLNLP 739
A I +AFP + CTVL+LP
Sbjct: 636 AKGITEAFPHMDCTVLDLP 692
>gb|DV465820.1|DV465820 MTUNUL1.P43.E12 NUL Populus fremontii x Populus angustifolia cDNA,
mRNA sequence
Length = 850
Score = 85.4 bits (180), Expect(3) = 7e-025
Identities = 36/75 (48%), Positives = 50/75 (66%)
Frame = +2 / +1
Query: 68 AELLQAQADLWRHSLYYLTSMALKCAVELHIPTAIHNLGGSATLPDLVAALSLPAAKLPF 247
+ELLQAQ +W H Y++ SM LKCAV+L IP I G TL +LV+AL + +K +
Sbjct: 55 SELLQAQTHVWNHIFYFINSMTLKCAVQLGIPDVIQKHGKPMTLSELVSALPIHPSKAQY 234
Query: 248 LGRVMRLLVTSGVFA 292
+ R+MR+LV SG F+
Sbjct: 235 VHRLMRILVHSGFFS 279
Score = 43.2 bits (88), Expect(3) = 7e-025
Identities = 15/23 (65%), Positives = 21/23 (91%)
Frame = +2 / +1
Query: 785 AQAVMLKLVLHFWSDDDCVKILE 853
A+A++LK +LH WSD+DCVKIL+
Sbjct: 778 AEAILLKWMLHNWSDEDCVKILK 846
Score = 29.5 bits (58), Expect(3) = 7e-025
Identities = 11/19 (57%), Positives = 14/19 (73%)
Frame = +2 / +1
Query: 683 AAAIVKAFPDIKCTVLNLP 739
A I +AFP + CTVL+LP
Sbjct: 646 AKGITEAFPHMDCTVLDLP 702
>gb|CV280707.1|CV280707 WS0137.B21_G07 PTxD-IL-FL-A-4 Populus trichocarpa x Populus deltoides
cDNA clone WS0137_G07 3', mRNA sequence
Length = 919
Score = 60.2 bits (125), Expect(3) = 2e-022
Identities = 24/53 (45%), Positives = 33/53 (62%)
Frame = +2 / -2
Query: 947 EAQLLMDMLMMVNTKGRQRGEDDWRHIFTKAGFSDYKVVKKIGARGVIEVYP* 1105
E QL D+ MMV G +R E +W +F AGF +YK+ +G R +IE+YP*
Sbjct: 315 ETQLFFDLQMMVVAGGMERNEKEWAKLFFDAGFLNYKIYPVLGPRALIELYP* 157
Score = 54.2 bits (112), Expect(3) = 2e-022
Identities = 19/32 (59%), Positives = 28/32 (87%)
Frame = +2 / -2
Query: 785 AQAVMLKLVLHFWSDDDCVKILELCRKAIPSR 880
A+A++LK +LH WSD+DCVKIL+ C++AI S+
Sbjct: 486 AEAILLKWILHNWSDEDCVKILKRCKQAIMSK 391
Score = 29.5 bits (58), Expect(3) = 2e-022
Identities = 11/19 (57%), Positives = 14/19 (73%)
Frame = +2 / -2
Query: 683 AAAIVKAFPDIKCTVLNLP 739
A I +AFP + CTVL+LP
Sbjct: 618 AKGITEAFPHMDCTVLDLP 562
>gb|CV280517.1|CV280517 WS0136.B21_J20 PTxD-IL-FL-A-4 Populus trichocarpa x Populus deltoides
cDNA clone WS0136_J20 3', mRNA sequence
Length = 893
Score = 60.2 bits (125), Expect(3) = 2e-022
Identities = 24/53 (45%), Positives = 33/53 (62%)
Frame = +2 / -2
Query: 947 EAQLLMDMLMMVNTKGRQRGEDDWRHIFTKAGFSDYKVVKKIGARGVIEVYP* 1105
E QL D+ MMV G +R E +W +F AGF +YK+ +G R +IE+YP*
Sbjct: 307 ETQLFFDLQMMVVAGGMERNEKEWAKLFFDAGFLNYKIYPVLGPRALIELYP* 149
Score = 54.2 bits (112), Expect(3) = 2e-022
Identities = 19/32 (59%), Positives = 28/32 (87%)
Frame = +2 / -2
Query: 785 AQAVMLKLVLHFWSDDDCVKILELCRKAIPSR 880
A+A++LK +LH WSD+DCVKIL+ C++AI S+
Sbjct: 478 AEAILLKWILHNWSDEDCVKILKRCKQAIMSK 383
Score = 29.5 bits (58), Expect(3) = 2e-022
Identities = 11/19 (57%), Positives = 14/19 (73%)
Frame = +2 / -2
Query: 683 AAAIVKAFPDIKCTVLNLP 739
A I +AFP + CTVL+LP
Sbjct: 610 AKGITEAFPHMDCTVLDLP 554
>gb|CK096481.1|CK096481 UB15CPH11.3pR Populus active cambium cDNA library Populus tremula
cDNA clone UB15CPH11 3', mRNA sequence
Length = 882
Score = 60.6 bits (126), Expect(3) = 2e-022
Identities = 24/53 (45%), Positives = 34/53 (64%)
Frame = +2 / -2
Query: 947 EAQLLMDMLMMVNTKGRQRGEDDWRHIFTKAGFSDYKVVKKIGARGVIEVYP* 1105
E QLL D+ MMV+ G +R E +W +F AGF +YK+ + R +IE+YP*
Sbjct: 341 ETQLLFDLHMMVDADGMERNEKEWAKLFFDAGFLNYKIHPVLATRALIELYP* 183
Score = 55.6 bits (115), Expect(3) = 2e-022
Identities = 20/32 (62%), Positives = 28/32 (87%)
Frame = +2 / -2
Query: 785 AQAVMLKLVLHFWSDDDCVKILELCRKAIPSR 880
A+A++LK +LH WSD+DCVKIL+ CR+AI S+
Sbjct: 512 AEAILLKWILHDWSDEDCVKILKRCRQAIMSK 417
Score = 27.6 bits (54), Expect(3) = 2e-022
Identities = 10/19 (52%), Positives = 13/19 (68%)
Frame = +2 / -2
Query: 683 AAAIVKAFPDIKCTVLNLP 739
A I +AFP + CTV +LP
Sbjct: 644 AKGIAEAFPHMDCTVFDLP 588
>gb|DT504273.1|DT504273 WS01312.B21_L07 PTxD-IL-FL-A-4 Populus trichocarpa x Populus
deltoides cDNA clone WS01312_L07 3', mRNA sequence
Length = 822
Score = 60.2 bits (125), Expect(3) = 2e-022
Identities = 24/53 (45%), Positives = 33/53 (62%)
Frame = +2 / -1
Query: 947 EAQLLMDMLMMVNTKGRQRGEDDWRHIFTKAGFSDYKVVKKIGARGVIEVYP* 1105
E QL D+ MMV G +R E +W +F AGF +YK+ +G R +IE+YP*
Sbjct: 336 ETQLFFDLQMMVVAGGMERNEKEWAKLFFDAGFLNYKIYPVLGPRALIELYP* 178
Score = 54.2 bits (112), Expect(3) = 2e-022
Identities = 19/32 (59%), Positives = 28/32 (87%)
Frame = +2 / -1
Query: 785 AQAVMLKLVLHFWSDDDCVKILELCRKAIPSR 880
A+A++LK +LH WSD+DCVKIL+ C++AI S+
Sbjct: 507 AEAILLKWILHNWSDEDCVKILKRCKQAIMSK 412
Score = 29.5 bits (58), Expect(3) = 2e-022
Identities = 11/19 (57%), Positives = 14/19 (73%)
Frame = +2 / -1
Query: 683 AAAIVKAFPDIKCTVLNLP 739
A I +AFP + CTVL+LP
Sbjct: 639 AKGITEAFPPMDCTVLDLP 583
>gb|DT476355.1|DT476355 WS01229.B21_E11 PT-GT-FL-A-3 Populus trichocarpa cDNA clone
WS01229_E11 3', mRNA sequence
Length = 887
Score = 59.7 bits (124), Expect(3) = 6e-022
Identities = 24/53 (45%), Positives = 33/53 (62%)
Frame = +2 / -2
Query: 947 EAQLLMDMLMMVNTKGRQRGEDDWRHIFTKAGFSDYKVVKKIGARGVIEVYP* 1105
E QL D+ MMV G +R E +W +F AGF +YK+ +G R +IE+YP*
Sbjct: 382 ETQLFFDLQMMVVVGGMERNEKEWAKLFFDAGFLNYKIHPVLGTRALIELYP* 224
Score = 53.3 bits (110), Expect(3) = 6e-022
Identities = 19/32 (59%), Positives = 28/32 (87%)
Frame = +2 / -2
Query: 785 AQAVMLKLVLHFWSDDDCVKILELCRKAIPSR 880
A+A++LK +LH WSD+DCVKIL+ C++AI S+
Sbjct: 553 AEAILLKWMLHNWSDEDCVKILKRCKQAIMSK 458
Score = 29.5 bits (58), Expect(3) = 6e-022
Identities = 11/19 (57%), Positives = 14/19 (73%)
Frame = +2 / -2
Query: 683 AAAIVKAFPDIKCTVLNLP 739
A I +AFP + CTVL+LP
Sbjct: 685 AKGITEAFPHMDCTVLDLP 629
>gb|CV235235.1|CV235235 WS01218.B21_E23 PT-GT-FL-A-3 Populus trichocarpa cDNA clone
WS01218_E23 3', mRNA sequence
Length = 714
Score = 59.7 bits (124), Expect(3) = 6e-022
Identities = 24/53 (45%), Positives = 33/53 (62%)
Frame = +2 / -1
Query: 947 EAQLLMDMLMMVNTKGRQRGEDDWRHIFTKAGFSDYKVVKKIGARGVIEVYP* 1105
E QL D+ MMV G +R E +W +F AGF +YK+ +G R +IE+YP*
Sbjct: 372 ETQLFFDLQMMVVVGGMERNEKEWAKLFFDAGFLNYKIHPVLGTRALIELYP* 214
Score = 53.3 bits (110), Expect(3) = 6e-022
Identities = 19/32 (59%), Positives = 28/32 (87%)
Frame = +2 / -1
Query: 785 AQAVMLKLVLHFWSDDDCVKILELCRKAIPSR 880
A+A++LK +LH WSD+DCVKIL+ C++AI S+
Sbjct: 543 AEAILLKWMLHNWSDEDCVKILKRCKQAIMSK 448
Score = 29.5 bits (58), Expect(3) = 6e-022
Identities = 11/19 (57%), Positives = 14/19 (73%)
Frame = +2 / -1
Query: 683 AAAIVKAFPDIKCTVLNLP 739
A I +AFP + CTVL+LP
Sbjct: 675 AKGITEAFPTMDCTVLDLP 619
>gb|DN488662.1|DN488662 S021B06.3pR Populus imbibed seed cDNA library Populus tremula cDNA
clone S021B06 3', mRNA sequence
Length = 568
Score = 71.2 bits (149), Expect(2) = 8e-021
Identities = 28/53 (52%), Positives = 36/53 (67%)
Frame = +2 / -3
Query: 947 EAQLLMDMLMMVNTKGRQRGEDDWRHIFTKAGFSDYKVVKKIGARGVIEVYP* 1105
E QL DMLMM+ G++R E +W +FT AGFS YK+ +G R +IEVYP*
Sbjct: 239 ETQLFFDMLMMILVTGKERNEKEWAKLFTDAGFSHYKINPVLGLRSLIEVYP* 81
Score = 50.1 bits (103), Expect(2) = 8e-021
Identities = 18/40 (45%), Positives = 29/40 (72%)
Frame = +2 / -3
Query: 785 AQAVMLKLVLHFWSDDDCVKILELCRKAIPSREEGGKVII 904
A A++LK +LH W++++CVKIL+ C++ I R G +II
Sbjct: 401 ADAILLKWILHDWNEEECVKILKQCKEEIKGRGGGKVIII 282
Database: Populus_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:49 PM
Number of letters in database: 203,408,664
Number of sequences in database: 369,679
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 522,987,219
Number of Sequences: 369679
Number of extensions: 6765994
Number of successful extensions: 39269
Number of sequences better than 1.0e-020: 10
length of database: 67,802,888
effective HSP length: 53
effective length of database: 48,209,901
effective search space used: 18705441588
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)