TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2455828.2.1
(1565 letters)
Database: Populus_nucl_with_EST.fasta
369,679 sequences; 203,408,664 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN517607.1|CN517607 GQ0091.B3_P01 GQ009 Populus trichoca... 135 2e-042
gb|CF235005.1|CF235005 PtaJXT0017G12G1214 Poplar cDNA libra... 129 1e-029
gb|CV274294.1|CV274294 WS0172.B21_N01 PTxD-NR-A-8 Populus t... 60 1e-022
>gb|CN517607.1|CN517607 GQ0091.B3_P01 GQ009 Populus trichocarpa x Populus deltoides cDNA
clone GQ0091_P01 5', mRNA sequence
Length = 570
Score = 135 bits (290), Expect(2) = 2e-042
Identities = 58/94 (61%), Positives = 68/94 (72%)
Frame = -2 / +2
Query: 595 DNEDTIKVVRSGGEDPDGRPGDLYVTLEVREDPVFRREKGDIHVDAVLNVTQAILGGTVQ 416
DNE G + +PGDLYVT++VREDPVFRRE +IHVDAVL +TQAILGGT+Q
Sbjct: 272 DNETMKLPRSGGADPEKNQPGDLYVTIKVREDPVFRREGSNIHVDAVLGITQAILGGTIQ 451
Query: 415 VPTLSGDVVLKIKPGTQPGQRVVLRGKGIKTRNS 314
VPTL+GDVVLK++PGTQ GQ L KG R S
Sbjct: 452 VPTLTGDVVLKVRPGTQXGQXXXLXNKGXXARAS 553
Score = 84.0 bits (177), Expect(2) = 3e-027
Identities = 30/58 (51%), Positives = 43/58 (74%)
Frame = -2 / +2
Query: 718 QSTCTKCGGSGKTVKEFCKACRGNKVVPGRKSVRLDILPGSDNEDTIKVVRSGGEDPD 545
Q TC +CGG+G+TV FC++C G KVV G K++ L+I G D+ +T+K+ RSGG DP+
Sbjct: 146 QQTCDQCGGTGQTVSSFCRSCHGRKVVRGSKTISLNIPTGVDDNETMKLPRSGGADPE 319
Score = 79.4 bits (167), Expect(2) = 9e-030
Identities = 44/95 (46%), Positives = 49/95 (51%)
Frame = +3 / -1
Query: 576 FMVSSLSEPGSMSRRTDFLPGTTLFPLHALQNSFTVFPEPPHFVQVDCILNGPEKKNINP 755
F+VS S P M F P TTL P LQ TV P PPH V L P +I P
Sbjct: 288 FIVSLSSTPVGMFNEIVFDPLTTLRPWQDLQKLETVCPVPPH*SHVC*KLKKPFCVDITP 109
Query: 756 EPLQDRHVSGVVPGGTPAPPQISHVTDVWKVTVLL 860
PLQ H+ G PGGTP+PPQ S T V V VL+
Sbjct: 108 LPLQRLHI*GFTPGGTPSPPQASQATSV*NVIVLV 4
Score = 72.1 bits (151), Expect(2) = 9e-030
Identities = 32/70 (45%), Positives = 43/70 (61%)
Frame = +2 / -3
Query: 323 CLDPFSSQNYPLTRLSTGLDL*NNVSTESWDLNCPSQNCLSHIQDSINMNITFLSSENRI 502
C PF Q LT L DL N+S ESW+L+ QNCL + +SINMN+ SSENR+
Sbjct: 544 CXXPFIXQXXXLTXLGARADLEYNISCESWNLDGSPQNCLGNA*NSINMNVRAFSSENRV 365
Query: 503 LTNLEGHIKI 532
+L+ +I+I
Sbjct: 364 FPDLDCNIEI 335
Score = 61.1 bits (127), Expect(2) = 2e-042
Identities = 20/30 (66%), Positives = 23/30 (76%)
Frame = -2 / +2
Query: 862 CSKTVTFQTSVTCEICGGAGVPPGTTPETC 773
C+KT+TFQT V CE CGG GVPPG P+ C
Sbjct: 2 CTKTITFQTDVACEACGGEGVPPGVKPQMC 91
Score = 59.3 bits (123), Expect(2) = 3e-027
Identities = 26/57 (45%), Positives = 32/57 (56%)
Frame = -1 / +1
Query: 527 LCDPRGS*GSCFPKRER*YSC*CCPECDSGNFGRDSSSPNSQWRRCSKDQARYSTWS 357
LC +G CFPKR *+SC*CC GNFG + S NS R C++ + TWS
Sbjct: 340 LCYNQGPGRPCFPKRRL*HSC*CCSRHYPGNFGGNHPSSNSHRRCCTQGPPWHPTWS 510
>gb|CF235005.1|CF235005 PtaJXT0017G12G1214 Poplar cDNA library from young tension xylem
Populus alba x Populus tremula cDNA 5', mRNA sequence
Length = 736
Score = 129 bits (277), Expect(2) = 1e-029
Identities = 54/72 (75%), Positives = 61/72 (84%)
Frame = -2 / +1
Query: 442 QAILGGTVQVPTLSGDVVLKIKPGTQPGQRVVLRGKGIKTRNSPYHGDQYVHFNVNIPEN 263
QAILGGT+QVPTL+GDVVLK++PGTQPGQ+VVL+ KGIK R S GDQ+VHFNV IP
Sbjct: 346 QAILGGTIQVPTLTGDVVLKVRPGTQPGQKVVLKNKGIKXRASYSFGDQFVHFNVKIPTT 525
Query: 262 LTSRQRELIEEF 227
LT RQ ELIEEF
Sbjct: 526 LTPRQCELIEEF 561
Score = 24.9 bits (48), Expect(2) = 1e-029
Identities = 9/12 (75%), Positives = 11/12 (91%)
Frame = -2 / +1
Query: 475 DIHVDAVLNVTQ 440
+IHVDAVL +TQ
Sbjct: 1 NIHVDAVLGITQ 36
>gb|CV274294.1|CV274294 WS0172.B21_N01 PTxD-NR-A-8 Populus trichocarpa x Populus deltoides
cDNA clone WS0172_N01 3', mRNA sequence
Length = 950
Score = 60.2 bits (125), Expect(3) = 1e-022
Identities = 26/83 (31%), Positives = 43/83 (51%)
Frame = -2 / +3
Query: 676 KEFCKACRGNKVVPGRKSVRLDILPGSDNEDTIKVVRSGGEDPDGRPGDLYVTLEVREDP 497
K+ C C+G KVV +K + + + G N I E PD GD+ L+ +E P
Sbjct: 675 KDRCPQCKGEKVVQEKKVLEVVVEKGMQNAQRITFPGEADEAPDTVTGDIVFVLQQKEHP 854
Query: 496 VFRREKGDIHVDAVLNVTQAILG 428
F+R+ D+ V+ L++ +A+ G
Sbjct: 855 KFKRKGDDLFVEHTLSLAEALCG 923
Score = 49.2 bits (101), Expect(3) = 1e-022
Identities = 20/30 (66%), Positives = 24/30 (80%)
Frame = -2 / +3
Query: 1264 YYDVLGVSKSASQAEIKKAYYGLAKKLHPD 1175
YY++LGVSKSASQ ++KKAY A K HPD
Sbjct: 96 YYEILGVSKSASQDDLKKAYRKAAIKNHPD 185
Score = 41.8 bits (85), Expect(3) = 1e-022
Identities = 15/27 (55%), Positives = 20/27 (74%)
Frame = -2 / +3
Query: 1144 KFQEVQRAYETLKDEQKRSLYDQVGPD 1064
KF+E+ +AYE L D +KR +YDQ G D
Sbjct: 204 KFKELAQAYEVLSDPEKREIYDQYGED 284
Database: Populus_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:49 PM
Number of letters in database: 203,408,664
Number of sequences in database: 369,679
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 730,155,046
Number of Sequences: 369679
Number of extensions: 10791467
Number of successful extensions: 58787
Number of sequences better than 1.0e-020: 5
length of database: 67,802,888
effective HSP length: 54
effective length of database: 47,840,222
effective search space used: 22341383674
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)