TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2441276.2.1
(1796 letters)
Database: Populus_nucl_with_EST.fasta
369,679 sequences; 203,408,664 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|DN496803.1|DN496803 Q019A06.5pR Populus dormant bud cDNA... 95 2e-039
gb|BU870838.1|BU870838 Q019A06 Populus flower cDNA library ... 92 2e-038
gb|DT469334.1|DT469334 WS01917.C21_H09 PT-DX-N-A-10 Populus... 92 3e-033
gb|BU879728.1|BU879728 V063F09 Populus flower cDNA library ... 95 6e-030
>gb|DN496803.1|DN496803 Q019A06.5pR Populus dormant bud cDNA library Populus tremula cDNA
clone Q019A06 5', mRNA sequence
Length = 651
Score = 94.5 bits (200), Expect(4) = 2e-039
Identities = 40/61 (65%), Positives = 49/61 (80%)
Frame = -2 / +3
Query: 1795 LFLRGDNGELRLGVRRAAQLKNGSAFPALYNQCSNLGSLPNVAHAVATKSVFHIYYNPRL 1616
LFLRG++GELRLGVRRAAQ+K G FPAL+NQ N SL +VA+A++ +S F IYYNPR
Sbjct: 129 LFLRGEDGELRLGVRRAAQVKCGPTFPALWNQQLNHSSLADVANAISMRSAFRIYYNPRA 308
Query: 1615 S 1613
S
Sbjct: 309 S 311
Score = 47.3 bits (97), Expect(4) = 2e-039
Identities = 17/31 (54%), Positives = 25/31 (80%)
Frame = -2 / +3
Query: 1552 AGSRFKVRYESDDASERRCTGIIAGIGDADP 1460
+G R K+R+E++DA+ERR TG+I GI + DP
Sbjct: 372 SGMRVKMRFETEDAAERRYTGLITGISELDP 464
Score = 42.8 bits (87), Expect(4) = 2e-039
Identities = 13/15 (86%), Positives = 14/15 (93%)
Frame = -2 / +3
Query: 1456 WRGSKWKCLMVRWDD 1412
W GSKWKCL+VRWDD
Sbjct: 471 WPGSKWKCLLVRWDD 515
Score = 35.0 bits (70), Expect(4) = 2e-039
Identities = 13/29 (44%), Positives = 21/29 (72%)
Frame = -2 / +3
Query: 1414 DDVDFRQPNRISPWEIELTSSVSGSHMSA 1328
DD++ + +R+SPWE+E + S SGS S+
Sbjct: 510 DDMEANRHSRVSPWEVEPSGSGSGSISSS 596
>gb|BU870838.1|BU870838 Q019A06 Populus flower cDNA library Populus trichocarpa cDNA 5 prime,
mRNA sequence
Length = 684
Score = 92.3 bits (195), Expect(4) = 2e-038
Identities = 39/61 (63%), Positives = 48/61 (78%)
Frame = -2 / +3
Query: 1795 LFLRGDNGELRLGVRRAAQLKNGSAFPALYNQCSNLGSLPNVAHAVATKSVFHIYYNPRL 1616
LFLRG++GELRLGVR AAQ+K G FPAL+NQ N SL +VA+A++ +S F IYYNPR
Sbjct: 105 LFLRGEDGELRLGVRTAAQVKCGPTFPALWNQHLNQSSLADVANAISMRSAFRIYYNPRA 284
Query: 1615 S 1613
S
Sbjct: 285 S 287
Score = 47.3 bits (97), Expect(4) = 2e-038
Identities = 17/31 (54%), Positives = 25/31 (80%)
Frame = -2 / +3
Query: 1552 AGSRFKVRYESDDASERRCTGIIAGIGDADP 1460
+G R K+R+E++DA+ERR TG+I GI + DP
Sbjct: 348 SGMRVKMRFETEDAAERRYTGLITGISELDP 440
Score = 42.8 bits (87), Expect(4) = 2e-038
Identities = 13/15 (86%), Positives = 14/15 (93%)
Frame = -2 / +3
Query: 1456 WRGSKWKCLMVRWDD 1412
W GSKWKCL+VRWDD
Sbjct: 447 WPGSKWKCLLVRWDD 491
Score = 34.1 bits (68), Expect(4) = 2e-038
Identities = 13/29 (44%), Positives = 20/29 (68%)
Frame = -2 / +3
Query: 1414 DDVDFRQPNRISPWEIELTSSVSGSHMSA 1328
DD++ +R+SPWE+E + S SGS S+
Sbjct: 486 DDMEANTHSRVSPWEVEPSGSGSGSISSS 572
>gb|DT469334.1|DT469334 WS01917.C21_H09 PT-DX-N-A-10 Populus trichocarpa cDNA clone
WS01917_H09 3', mRNA sequence
Length = 716
Score = 91.8 bits (194), Expect(2) = 3e-033
Identities = 33/75 (44%), Positives = 49/75 (65%)
Frame = -2 / +3
Query: 1549 GSRFKVRYESDDASERRCTGIIAGIGDADPMWRGSKWKCLMVRWDDDVDFRQPNRISPWE 1370
G RFK+R+E +D+ ERR +G I G+ D P W SKW+ L V+WD+ +P+R+SPWE
Sbjct: 345 GMRFKMRFEGEDSPERRFSGTIVGVEDFSPHWNDSKWRSLKVQWDEPASISRPDRVSPWE 524
Query: 1369 IELTSSVSGSHMSAP 1325
IE + +++S P
Sbjct: 525 IEPCVASVPANLSQP 569
Score = 71.6 bits (150), Expect(2) = 3e-033
Identities = 29/62 (46%), Positives = 43/62 (69%)
Frame = -2 / +3
Query: 1795 LFLRGDNGELRLGVRRAAQLKNGSAFPALYNQCSNLGSLPNVAHAVATKSVFHIYYNPRL 1616
+FLRG+NGELR+GVRR A+ ++ + +Q +LG L +HAV+T ++F +YY PR
Sbjct: 105 VFLRGENGELRVGVRRVARQQSSMPSSVISSQSMHLGVLATASHAVSTLTLFVVYYKPRT 284
Query: 1615 SQ 1610
SQ
Sbjct: 285 SQ 290
>gb|BU879728.1|BU879728 V063F09 Populus flower cDNA library Populus trichocarpa cDNA 5 prime,
mRNA sequence
Length = 503
Score = 94.5 bits (200), Expect(2) = 6e-030
Identities = 33/82 (40%), Positives = 53/82 (64%)
Frame = -2 / +1
Query: 1549 GSRFKVRYESDDASERRCTGIIAGIGDADPMWRGSKWKCLMVRWDDDVDFRQPNRISPWE 1370
G RFK+R+E +D+ ERR TG I G+GD P W GS W+ L ++WD+ ++P R+SPW+
Sbjct: 232 GMRFKMRFEGEDSPERRFTGTIVGVGDISPEWSGSIWRSLKIQWDEPATIQRPERVSPWD 411
Query: 1369 IELTSSVSGSHMSAPNAKRLKP 1304
IE ++ + +++ K +P
Sbjct: 412 IEPFAAPASPNLTQQVMKIKRP 477
Score = 57.9 bits (120), Expect(2) = 6e-030
Identities = 24/59 (40%), Positives = 37/59 (62%)
Frame = -2 / +1
Query: 1786 RGDNGELRLGVRRAAQLKNGSAFPALYNQCSNLGSLPNVAHAVATKSVFHIYYNPRLSQ 1610
RG N ELR+GVRR A+ ++ + +Q +LG L +H V T+++F +YY PR +Q
Sbjct: 1 RGHNRELRVGVRRLARQQSSIPSSVISSQSMHLGVLATASHTVLTQTLFVVYYKPRTNQ 177
Database: Populus_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:49 PM
Number of letters in database: 203,408,664
Number of sequences in database: 369,679
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 992,675,007
Number of Sequences: 369679
Number of extensions: 15924054
Number of successful extensions: 71772
Number of sequences better than 1.0e-020: 4
length of database: 67,802,888
effective HSP length: 54
effective length of database: 47,840,222
effective search space used: 26025080768
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)