TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2440450.2.1
(1291 letters)
Database: Populus_nucl_with_EST.fasta
369,679 sequences; 203,408,664 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|DT510682.1|DT510682 WS02427.BR_E06 PTxD-ICC-N-A-14 Popul... 114 2e-032
gb|DT509290.1|DT509290 WS02423.BR_F06 PTxD-ICC-N-A-14 Popul... 114 8e-032
gb|AJ780212.1|AJ780212 AJ780212 Populus euphratica root 3-6... 120 6e-030
gb|CV130483.1|CV130483 X9P02b01 Populus stem seasonal libra... 117 4e-029
gb|CK087628.1|CK087628 A016P18.3pR Hybrid aspen plasmid lib... 93 7e-029
gb|AJ779275.1|AJ779275 AJ779275 Populus euphratica root 3-6... 116 7e-029
gb|DT513902.1|DT513902 WS02423.B21_F06 PTxD-ICC-N-A-14 Popu... 114 1e-028
gb|DT470198.1|DT470198 WS01920.C21_C12 PT-DX-N-A-10 Populus... 114 1e-028
gb|DT515327.1|DT515327 WS02427.B21_E06 PTxD-ICC-N-A-14 Popu... 114 1e-028
gb|BU888527.1|BU888527 P009B08 Populus petioles cDNA librar... 115 1e-028
gb|BU868792.1|BU868792 M122B10 Populus flower cDNA library ... 114 2e-028
gb|CV243510.1|CV243510 WS0252.B21_B05 PT-MB-N-A-15 Populus ... 95 7e-023
gb|AI162343.1|AI162343 A016P18U Hybrid aspen plasmid librar... 98 7e-022
>gb|DT510682.1|DT510682 WS02427.BR_E06 PTxD-ICC-N-A-14 Populus trichocarpa x Populus
deltoides cDNA clone WS02427_E06 5', mRNA sequence
Length = 864
Score = 114 bits (244), Expect(3) = 2e-032
Identities = 45/108 (41%), Positives = 71/108 (65%)
Frame = -2 / +2
Query: 990 SGDRSNFAINGVEVSTIRLVGRMLGKVERVTDVVFTLDDGTGKIDVNRWENEASDAKEMA 811
S + SNF I+G EV+ + +VGR+ K ++ ++ F +DDGTG+I+ +W E+ D ++M
Sbjct: 170 SNNESNFIIDGAEVNNVTIVGRVSHKEDKASEYTFLVDDGTGQIECTKWVQESLDTEQMG 349
Query: 810 DANNENYVIVIGGLKGFQGKRHVVAYAVRRVTNFNEITCHFLHCIQVH 667
+ YV V G L+G QG+R + +++R VT+FNEI HF+ CI VH
Sbjct: 350 EILVGMYVRVHGHLRGLQGRRFLNVFSIRPVTDFNEIPGHFIECIYVH 493
Score = 36.3 bits (73), Expect(3) = 2e-032
Identities = 17/47 (36%), Positives = 26/47 (55%)
Frame = -2 / +2
Query: 510 GLHIKDVIDRLKLPDDVVSAAIQGHVDVGNIYNTIDDFHYKSVRNG* 370
G H + +L +P + + +Q VD G +Y TI+D +YKS N *
Sbjct: 683 GAHYDVIARQLNIPMNKLKEELQMLVDNGLVYTTINDDYYKSTVNA* 823
Score = 32.2 bits (64), Expect(3) = 2e-032
Identities = 13/29 (44%), Positives = 19/29 (65%)
Frame = -2 / +2
Query: 1074 ENSGLSKGRSAQTLLPLTVKQTMDAAQTS 988
++S +SK R A+ L PLTVKQ + A +
Sbjct: 92 DSSSISKNREARCLFPLTVKQISNLASNN 178
>gb|DT509290.1|DT509290 WS02423.BR_F06 PTxD-ICC-N-A-14 Populus trichocarpa x Populus
deltoides cDNA clone WS02423_F06 5', mRNA sequence
Length = 819
Score = 114 bits (244), Expect(3) = 8e-032
Identities = 45/108 (41%), Positives = 71/108 (65%)
Frame = -2 / +1
Query: 990 SGDRSNFAINGVEVSTIRLVGRMLGKVERVTDVVFTLDDGTGKIDVNRWENEASDAKEMA 811
S + SNF I+G EV+ + +VGR+ K ++ ++ F +DDGTG+I+ +W E+ D ++M
Sbjct: 178 SNNESNFIIDGAEVNNVTIVGRVSHKEDKASEYTFLVDDGTGQIECTKWVQESLDTEQMG 357
Query: 810 DANNENYVIVIGGLKGFQGKRHVVAYAVRRVTNFNEITCHFLHCIQVH 667
+ YV V G L+G QG+R + +++R VT+FNEI HF+ CI VH
Sbjct: 358 EILVGMYVRVHGHLRGLQGRRFLNVFSIRPVTDFNEIPGHFIECIYVH 501
Score = 34.5 bits (69), Expect(3) = 8e-032
Identities = 15/42 (35%), Positives = 24/42 (57%)
Frame = -2 / +1
Query: 510 GLHIKDVIDRLKLPDDVVSAAIQGHVDVGNIYNTIDDFHYKS 385
G H + +L +P + + +Q VD G +Y TI+D +YKS
Sbjct: 691 GAHYDVIARQLNIPMNKLKEELQMLVDNGLVYTTINDDYYKS 816
Score = 32.2 bits (64), Expect(3) = 8e-032
Identities = 13/29 (44%), Positives = 19/29 (65%)
Frame = -2 / +1
Query: 1074 ENSGLSKGRSAQTLLPLTVKQTMDAAQTS 988
++S +SK R A+ L PLTVKQ + A +
Sbjct: 100 DSSSISKNREARCLFPLTVKQISNLASNN 186
>gb|AJ780212.1|AJ780212 AJ780212 Populus euphratica root 3-6 months Populus euphratica cDNA
clone P0000800013H09F1, mRNA sequence
Length = 535
Score = 120 bits (256), Expect(2) = 6e-030
Identities = 47/112 (41%), Positives = 73/112 (65%)
Frame = -2 / +1
Query: 990 SGDRSNFAINGVEVSTIRLVGRMLGKVERVTDVVFTLDDGTGKIDVNRWENEASDAKEMA 811
S D SNF I+G EV+ + +VGR+ K ++ ++ F +DDGTG+I+ +W E+ D ++M
Sbjct: 199 SNDESNFIIDGAEVNNVTIVGRVSHKEDKASEYTFLVDDGTGQIECTKWVQESLDTEQMG 378
Query: 810 DANNENYVIVIGGLKGFQGKRHVVAYAVRRVTNFNEITCHFLHCIQVHMELP 655
+ YV V G L+G QG+R + +++R VT+FNEI HF+ CI VH +P
Sbjct: 379 EILVGMYVRVHGHLRGLQGRRFLNVFSIRPVTDFNEIPGHFIECIYVHFYIP 534
Score = 31.8 bits (63), Expect(2) = 6e-030
Identities = 13/26 (50%), Positives = 18/26 (69%)
Frame = -2 / +1
Query: 1074 ENSGLSKGRSAQTLLPLTVKQTMDAA 997
++S +SK R A+ L PLTVKQ + A
Sbjct: 121 DSSSISKNREARCLFPLTVKQISNLA 198
>gb|CV130483.1|CV130483 X9P02b01 Populus stem seasonal library Populus deltoides cDNA, mRNA
sequence
Length = 789
Score = 117 bits (250), Expect(2) = 4e-029
Identities = 46/108 (42%), Positives = 71/108 (65%)
Frame = -2 / +2
Query: 990 SGDRSNFAINGVEVSTIRLVGRMLGKVERVTDVVFTLDDGTGKIDVNRWENEASDAKEMA 811
S D SNF I+G EV+ + +VGR+ K ++ ++ F +DDGTG+I+ +W E+ D ++M
Sbjct: 233 SNDESNFTIDGAEVNNVTIVGRVSHKEDKASEYTFLVDDGTGQIECTKWVQESLDTEQMG 412
Query: 810 DANNENYVIVIGGLKGFQGKRHVVAYAVRRVTNFNEITCHFLHCIQVH 667
+ YV V G L+G QG+R + +++R VT+FNEI HF+ CI VH
Sbjct: 413 EILVGMYVRVHGHLRGLQGRRFLNVFSIRPVTDFNEIPGHFIECIYVH 556
Score = 31.8 bits (63), Expect(2) = 4e-029
Identities = 13/26 (50%), Positives = 18/26 (69%)
Frame = -2 / +2
Query: 1074 ENSGLSKGRSAQTLLPLTVKQTMDAA 997
++S +SK R A+ L PLTVKQ + A
Sbjct: 155 DSSSISKNREARCLFPLTVKQISNLA 232
>gb|CK087628.1|CK087628 A016P18.3pR Hybrid aspen plasmid library Populus tremula x Populus
tremuloides cDNA clone A016P18 3', mRNA sequence
Length = 779
Score = 93.2 bits (197), Expect(2) = 7e-029
Identities = 39/79 (49%), Positives = 51/79 (64%)
Frame = -2 / -2
Query: 897 DVVFTLDDGTGKIDVNRWENEASDAKEMADANNENYVIVIGGLKGFQGKRHVVAYAVRRV 718
DV F +DDGT +I RW E D EM +E YV VIG L+ FQ + +VA++VR V
Sbjct: 778 DVSFVIDDGTARIGCRRWVTENFDKLEMEAVQDEMYVRVIGHLRVFQDVKQLVAFSVRPV 599
Query: 717 TNFNEITCHFLHCIQVHME 661
TNF+EIT HF+ CI H++
Sbjct: 598 TNFDEITFHFIDCIHSHLQ 542
Score = 58.3 bits (121), Expect(2) = 7e-029
Identities = 24/58 (41%), Positives = 37/58 (63%)
Frame = -2 / -2
Query: 558 LVLSVFHDPSVMDQEHGLHIKDVIDRLKLPDDVVSAAIQGHVDVGNIYNTIDDFHYKS 385
LVL S + QE G+H+ ++ +LKLP + + +I+ D G IY+TID+FHYK+
Sbjct: 397 LVLDRLQQSSSIGQEKGMHMDELCQQLKLPMEKIKESIRSLEDEGLIYSTIDEFHYKA 224
>gb|AJ779275.1|AJ779275 AJ779275 Populus euphratica root 3-6 months Populus euphratica cDNA
clone P0000800001A01F1, mRNA sequence
Length = 790
Score = 116 bits (248), Expect(2) = 7e-029
Identities = 46/108 (42%), Positives = 71/108 (65%)
Frame = -2 / +2
Query: 990 SGDRSNFAINGVEVSTIRLVGRMLGKVERVTDVVFTLDDGTGKIDVNRWENEASDAKEMA 811
S D SNF I+G EV+ + +VGR+ K ++ ++ F +DDGTG+I+ +W E+ D ++M
Sbjct: 236 SNDESNFIIDGAEVNNVTIVGRVSHKEDKASEYTFLVDDGTGQIECTKWVQESLDTEQMG 415
Query: 810 DANNENYVIVIGGLKGFQGKRHVVAYAVRRVTNFNEITCHFLHCIQVH 667
+ YV V G L+G QG+R + +++R VT+FNEI HF+ CI VH
Sbjct: 416 EILVGMYVRVHGHLRGLQGRRFLNIFSIRPVTDFNEIPGHFIECIYVH 559
Score = 31.8 bits (63), Expect(2) = 7e-029
Identities = 13/26 (50%), Positives = 18/26 (69%)
Frame = -2 / +2
Query: 1074 ENSGLSKGRSAQTLLPLTVKQTMDAA 997
++S +SK R A+ L PLTVKQ + A
Sbjct: 158 DSSSISKNREARCLFPLTVKQISNLA 235
>gb|DT513902.1|DT513902 WS02423.B21_F06 PTxD-ICC-N-A-14 Populus trichocarpa x Populus
deltoides cDNA clone WS02423_F06 3', mRNA sequence
Length = 873
Score = 114 bits (244), Expect(2) = 1e-028
Identities = 45/108 (41%), Positives = 71/108 (65%)
Frame = -2 / -3
Query: 990 SGDRSNFAINGVEVSTIRLVGRMLGKVERVTDVVFTLDDGTGKIDVNRWENEASDAKEMA 811
S + SNF I+G EV+ + +VGR+ K ++ ++ F +DDGTG+I+ +W E+ D ++M
Sbjct: 838 SNNESNFIIDGAEVNNVTIVGRVSHKEDKASEYTFLVDDGTGQIECTKWVQESLDTEQMG 659
Query: 810 DANNENYVIVIGGLKGFQGKRHVVAYAVRRVTNFNEITCHFLHCIQVH 667
+ YV V G L+G QG+R + +++R VT+FNEI HF+ CI VH
Sbjct: 658 EILVGMYVRVHGHLRGLQGRRFLNVFSIRPVTDFNEIPGHFIECIYVH 515
Score = 36.3 bits (73), Expect(2) = 1e-028
Identities = 17/47 (36%), Positives = 26/47 (55%)
Frame = -2 / -3
Query: 510 GLHIKDVIDRLKLPDDVVSAAIQGHVDVGNIYNTIDDFHYKSVRNG* 370
G H + +L +P + + +Q VD G +Y TI+D +YKS N *
Sbjct: 325 GAHYDVIARQLNIPMNKLKEELQMLVDNGLVYTTINDDYYKSTVNA* 185
>gb|DT470198.1|DT470198 WS01920.C21_C12 PT-DX-N-A-10 Populus trichocarpa cDNA clone
WS01920_C12 3', mRNA sequence
Length = 880
Score = 114 bits (244), Expect(2) = 1e-028
Identities = 45/108 (41%), Positives = 71/108 (65%)
Frame = -2 / -3
Query: 990 SGDRSNFAINGVEVSTIRLVGRMLGKVERVTDVVFTLDDGTGKIDVNRWENEASDAKEMA 811
S + SNF I+G EV+ + +VGR+ K ++ ++ F +DDGTG+I+ +W E+ D ++M
Sbjct: 845 SNNESNFIIDGAEVNNVTIVGRVSHKEDKASEYTFLVDDGTGQIECTKWVQESLDTEQMG 666
Query: 810 DANNENYVIVIGGLKGFQGKRHVVAYAVRRVTNFNEITCHFLHCIQVH 667
+ YV V G L+G QG+R + +++R VT+FNEI HF+ CI VH
Sbjct: 665 EILVGMYVRVHGHLRGLQGRRFLNVFSIRPVTDFNEIPGHFIECIYVH 522
Score = 36.3 bits (73), Expect(2) = 1e-028
Identities = 17/47 (36%), Positives = 26/47 (55%)
Frame = -2 / -3
Query: 510 GLHIKDVIDRLKLPDDVVSAAIQGHVDVGNIYNTIDDFHYKSVRNG* 370
G H + +L +P + + +Q VD G +Y TI+D +YKS N *
Sbjct: 332 GAHYDVIARQLNIPMNKLKEELQMLVDNGLVYTTINDDYYKSTVNA* 192
>gb|DT515327.1|DT515327 WS02427.B21_E06 PTxD-ICC-N-A-14 Populus trichocarpa x Populus
deltoides cDNA clone WS02427_E06 3', mRNA sequence
Length = 884
Score = 114 bits (244), Expect(2) = 1e-028
Identities = 45/108 (41%), Positives = 71/108 (65%)
Frame = -2 / -2
Query: 990 SGDRSNFAINGVEVSTIRLVGRMLGKVERVTDVVFTLDDGTGKIDVNRWENEASDAKEMA 811
S + SNF I+G EV+ + +VGR+ K ++ ++ F +DDGTG+I+ +W E+ D ++M
Sbjct: 838 SNNESNFIIDGAEVNNVTIVGRVSHKEDKASEYTFLVDDGTGQIECTKWVQESLDTEQMG 659
Query: 810 DANNENYVIVIGGLKGFQGKRHVVAYAVRRVTNFNEITCHFLHCIQVH 667
+ YV V G L+G QG+R + +++R VT+FNEI HF+ CI VH
Sbjct: 658 EILVGMYVRVHGHLRGLQGRRFLNVFSIRPVTDFNEIPGHFIECIYVH 515
Score = 36.3 bits (73), Expect(2) = 1e-028
Identities = 17/47 (36%), Positives = 26/47 (55%)
Frame = -2 / -2
Query: 510 GLHIKDVIDRLKLPDDVVSAAIQGHVDVGNIYNTIDDFHYKSVRNG* 370
G H + +L +P + + +Q VD G +Y TI+D +YKS N *
Sbjct: 325 GAHYDVIARQLNIPMNKLKEELQMLVDNGLVYTTINDDYYKSTVNA* 185
>gb|BU888527.1|BU888527 P009B08 Populus petioles cDNA library Populus tremula cDNA 5 prime,
mRNA sequence
Length = 655
Score = 115 bits (246), Expect(2) = 1e-028
Identities = 45/108 (41%), Positives = 71/108 (65%)
Frame = -2 / +1
Query: 990 SGDRSNFAINGVEVSTIRLVGRMLGKVERVTDVVFTLDDGTGKIDVNRWENEASDAKEMA 811
S D SNF I+G EV+ + ++GR+ K ++ ++ F +DDGTG+I+ +W E+ D ++M
Sbjct: 205 SNDESNFIIDGAEVNNVTILGRVSHKEDKASEYTFLVDDGTGQIECTKWVQESFDTEQMG 384
Query: 810 DANNENYVIVIGGLKGFQGKRHVVAYAVRRVTNFNEITCHFLHCIQVH 667
+ YV V G L+G QG+R + +++R VT+FNEI HF+ CI VH
Sbjct: 385 EILVGMYVRVHGHLRGLQGRRFLNVFSIRPVTDFNEIPGHFIECIYVH 528
Score = 31.8 bits (63), Expect(2) = 1e-028
Identities = 13/26 (50%), Positives = 18/26 (69%)
Frame = -2 / +1
Query: 1074 ENSGLSKGRSAQTLLPLTVKQTMDAA 997
++S +SK R A+ L PLTVKQ + A
Sbjct: 127 DSSSISKNREARCLFPLTVKQISNLA 204
>gb|BU868792.1|BU868792 M122B10 Populus flower cDNA library Populus trichocarpa cDNA 5
prime, mRNA sequence
Length = 691
Score = 114 bits (244), Expect(2) = 2e-028
Identities = 45/108 (41%), Positives = 71/108 (65%)
Frame = -2 / +3
Query: 990 SGDRSNFAINGVEVSTIRLVGRMLGKVERVTDVVFTLDDGTGKIDVNRWENEASDAKEMA 811
S + SNF I+G EV+ + +VGR+ K ++ ++ F +DDGTG+I+ +W E+ D ++M
Sbjct: 213 SNNESNFIIDGAEVNNVTIVGRVSHKEDKASEYTFLVDDGTGQIECTKWVQESLDTEQMG 392
Query: 810 DANNENYVIVIGGLKGFQGKRHVVAYAVRRVTNFNEITCHFLHCIQVH 667
+ YV V G L+G QG+R + +++R VT+FNEI HF+ CI VH
Sbjct: 393 EILVGMYVRVHGHLRGLQGRRFLNVFSIRPVTDFNEIPGHFIECIYVH 536
Score = 32.2 bits (64), Expect(2) = 2e-028
Identities = 13/29 (44%), Positives = 19/29 (65%)
Frame = -2 / +3
Query: 1074 ENSGLSKGRSAQTLLPLTVKQTMDAAQTS 988
++S +SK R A+ L PLTVKQ + A +
Sbjct: 135 DSSSISKNREARCLFPLTVKQISNLASNN 221
>gb|CV243510.1|CV243510 WS0252.B21_B05 PT-MB-N-A-15 Populus trichocarpa cDNA clone
WS0252_B05 3', mRNA sequence
Length = 793
Score = 95.0 bits (201), Expect(2) = 7e-023
Identities = 37/90 (41%), Positives = 59/90 (65%)
Frame = -2 / -2
Query: 936 LVGRMLGKVERVTDVVFTLDDGTGKIDVNRWENEASDAKEMADANNENYVIVIGGLKGFQ 757
+VGR+ K ++ ++ F +DDGTG+I+ +W E+ D ++M + YV V G L+G Q
Sbjct: 792 IVGRVSHKEDKASEYTFLVDDGTGQIECTKWVQESLDTEQMGEILVGMYVRVHGHLRGLQ 613
Query: 756 GKRHVVAYAVRRVTNFNEITCHFLHCIQVH 667
G+R + +++R VT+FNEI HF+ CI VH
Sbjct: 612 GRRFLNVFSIRPVTDFNEIPGHFIECIYVH 523
Score = 36.3 bits (73), Expect(2) = 7e-023
Identities = 17/47 (36%), Positives = 26/47 (55%)
Frame = -2 / -2
Query: 510 GLHIKDVIDRLKLPDDVVSAAIQGHVDVGNIYNTIDDFHYKSVRNG* 370
G H + +L +P + + +Q VD G +Y TI+D +YKS N *
Sbjct: 333 GAHYDVIARQLNIPMNKLKEELQMLVDNGLVYTTINDDYYKSTVNA* 193
>gb|AI162343.1|AI162343 A016P18U Hybrid aspen plasmid library Populus tremula x Populus
tremuloides cDNA 5', mRNA sequence
Length = 712
Score = 98.2 bits (208), Expect(2) = 7e-022
Identities = 41/80 (51%), Positives = 53/80 (66%)
Frame = -2 / +1
Query: 900 TDVVFTLDDGTGKIDVNRWENEASDAKEMADANNENYVIVIGGLKGFQGKRHVVAYAVRR 721
TDV F +DDGTG+I RW E D EM +E YV VIG L+ FQ + +VA++VR
Sbjct: 1 TDVSFVIDDGTGRIGCRRWVTENFDKLEMEAVQDEMYVRVIGHLRVFQDVKQLVAFSVRP 180
Query: 720 VTNFNEITCHFLHCIQVHME 661
VTNF+EIT HF+ CI H++
Sbjct: 181 VTNFDEITFHFIDCIHSHLQ 240
Score = 29.5 bits (58), Expect(2) = 7e-022
Identities = 12/30 (40%), Positives = 18/30 (60%)
Frame = -2 / +1
Query: 558 LVLSVFHDPSVMDQEHGLHIKDVIDRLKLP 469
LVL S + QE G+H+ ++ +LKLP
Sbjct: 385 LVLDRLQQSSSIGQEKGMHMDELCQQLKLP 474
Database: Populus_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:49 PM
Number of letters in database: 203,408,664
Number of sequences in database: 369,679
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 557,217,273
Number of Sequences: 369679
Number of extensions: 7828289
Number of successful extensions: 39280
Number of sequences better than 1.0e-020: 15
length of database: 67,802,888
effective HSP length: 53
effective length of database: 48,209,901
effective search space used: 18126922776
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)