TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2405087.2.1
(737 letters)
Database: Populus_nucl_with_EST.fasta
369,679 sequences; 203,408,664 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BU835835.1|BU835835 T079C04 Populus apical shoot cDNA li... 147 4e-043
gb|CX182313.1|CX182313 D02_45-83_08.ab1 leaf inoculated wit... 104 3e-042
gb|CK103107.1|CK103107 G103P72.5pR Populus tension wood cDN... 147 1e-041
gb|BI130274.1|BI130274 G103P25Y Populus cambium cDNA librar... 91 8e-039
gb|BU885401.1|BU885401 R030G01 Populus root cDNA library Po... 104 1e-032
gb|DT483569.1|DT483569 WS02522.B21_P13 PT-MB-N-A-15 Populus... 102 5e-028
gb|DT488170.1|DT488170 WS02535.B21_L05 PT-MB-N-A-15 Populus... 101 6e-021
>gb|BU835835.1|BU835835 T079C04 Populus apical shoot cDNA library Populus tremula x Populus
tremuloides cDNA 5 prime, mRNA sequence
Length = 673
Score = 147 bits (315), Expect(2) = 4e-043
Identities = 60/115 (52%), Positives = 82/115 (71%)
Frame = +2 / +1
Query: 392 TSNNSKITFALDQNHQSHIEYRWGGEGLRKYLVKWATSVVERKGRTERQIIFEDPEHSSA 571
T N F +D + SHIEYRWGGEGLRK LV+WA+SV ++K E +I+ + SS
Sbjct: 106 TPNPEDGPFVVDFYYHSHIEYRWGGEGLRKTLVRWASSVSDKKAENEERIVTAAEQLSSD 285
Query: 572 YCLAFRVVNPNHLPPLKELRKLMRIQSLRCNALYNHSATRLSVVPIHASRSQALR 736
YC AF V P +PP+K+L+K++RIQ+LRC+ +Y + TRL+V+P+ ASRSQALR
Sbjct: 286 YCYAFTVKKPGLVPPVKDLQKVLRIQALRCHVIYCQNGTRLNVIPVLASRSQALR 450
Score = 47.8 bits (98), Expect(2) = 4e-043
Identities = 17/27 (62%), Positives = 22/27 (81%)
Frame = +2 / +1
Query: 311 GGMLPTDFDAFICNSGSNIYYPSHSRE 391
GG P+DFDAFICNSGS++YY + + E
Sbjct: 40 GGFSPSDFDAFICNSGSDLYYSTPNPE 120
>gb|CX182313.1|CX182313 D02_45-83_08.ab1 leaf inoculated with Marssonia pathogen of Populus
euramericana Populus x canadensis cDNA, mRNA sequence
Length = 648
Score = 104 bits (222), Expect(2) = 3e-042
Identities = 45/83 (54%), Positives = 55/83 (66%)
Frame = +2 / +1
Query: 143 RKNIIVISVDSVNKEGLVRIIRNAIEVIHKENMSGSTGFVLSTSLTISEIHSLLLSGGML 322
RK I V++VD N L R E + KE + GS GF+LSTSL ISEI S L SGG
Sbjct: 190 RKQIFVVAVDFDNFASLAEATRKIFEAVEKERVEGSIGFILSTSLAISEICSFLASGGFS 369
Query: 323 PTDFDAFICNSGSNIYYPSHSRE 391
P+DFDAFICNSGS++YY + + E
Sbjct: 370 PSDFDAFICNSGSDLYYSTPNPE 438
Score = 87.7 bits (185), Expect(2) = 3e-042
Identities = 33/75 (44%), Positives = 47/75 (62%)
Frame = +2 / +1
Query: 392 TSNNSKITFALDQNHQSHIEYRWGGEGLRKYLVKWATSVVERKGRTERQIIFEDPEHSSA 571
T N F +D + SHIEYRWGGEGLRK L +W++SV+++K +I++ + S+
Sbjct: 424 TPNPEDGPFVIDFYYHSHIEYRWGGEGLRKTLFRWSSSVIDKKAEDAERIVYSAEQLSTD 603
Query: 572 YCLAFRVVNPNHLPP 616
YC AF V P +PP
Sbjct: 604 YCYAFTVKKPGSVPP 648
Score = 76.2 bits (160), Expect(2) = 1e-021
Identities = 35/73 (47%), Positives = 47/73 (64%)
Frame = -3 / -2
Query: 360 LPLLQMKASKSVGSMPPDSSSECISDIVKEVDSTKPVEPDMFSLCMTSIAFLIILTRPSL 181
LPLLQ+KASKS+G PP++ E ISD+ K+VD+ P+EP S S FL+ R +
Sbjct: 407 LPLLQIKASKSLGLNPPEARKEHISDMAKDVDNINPIEPSTRSFSTASKIFLVASARLAK 228
Query: 180 LTESTEITMIFFL 142
L++ST T I FL
Sbjct: 227 LSKSTATTKICFL 189
Score = 46.9 bits (96), Expect(2) = 1e-021
Identities = 25/59 (42%), Positives = 31/59 (52%)
Frame = -3 / -2
Query: 615 GGR*LGLTTLNARQYAEECSGSSKIICLSVLPFLSTTEVAHFTRYFLRPSPPQRYSICD 439
GG G T+ A Q + E + I + FLS TE R FL PSPPQRYS+C+
Sbjct: 647 GGTDPGFFTVKA*Q*SVESCSAEYTIRSASSAFLSITEDDQRNRVFLSPSPPQRYSMCE 471
>gb|CK103107.1|CK103107 G103P72.5pR Populus tension wood cDNA library Populus tremula x
Populus tremuloides cDNA clone G103P72 5', mRNA sequence
Length = 597
Score = 147 bits (315), Expect(2) = 1e-041
Identities = 60/115 (52%), Positives = 82/115 (71%)
Frame = +2 / +1
Query: 392 TSNNSKITFALDQNHQSHIEYRWGGEGLRKYLVKWATSVVERKGRTERQIIFEDPEHSSA 571
T N F +D + SHIEYRWGGEGLRK LV+WA+SV ++K E +I+ + S+
Sbjct: 61 TPNPEDGPFVVDFYYHSHIEYRWGGEGLRKTLVRWASSVSDKKAENEERIVTAAEQLSTD 240
Query: 572 YCLAFRVVNPNHLPPLKELRKLMRIQSLRCNALYNHSATRLSVVPIHASRSQALR 736
YC AF V P +PP+KEL+K++RIQ+LRC+ +Y + TRL+V+P+ ASRSQALR
Sbjct: 241 YCYAFTVKKPGLVPPVKELQKVLRIQALRCHVIYCQNGTRLNVIPVLASRSQALR 405
Score = 43.2 bits (88), Expect(2) = 1e-041
Identities = 15/23 (65%), Positives = 20/23 (86%)
Frame = +2 / +1
Query: 323 PTDFDAFICNSGSNIYYPSHSRE 391
P+DFDAFICNSGS++YY + + E
Sbjct: 7 PSDFDAFICNSGSDLYYSTPNPE 75
>gb|BI130274.1|BI130274 G103P25Y Populus cambium cDNA library Populus tremula x Populus
tremuloides cDNA, mRNA sequence
Length = 425
Score = 91.3 bits (193), Expect(3) = 8e-039
Identities = 37/74 (50%), Positives = 53/74 (71%)
Frame = +2 / +2
Query: 515 RKGRTERQIIFEDPEHSSAYCLAFRVVNPNHLPPLKELRKLMRIQSLRCNALYNHSATRL 694
RK E +I+ + S+ YC AF V P +PP+KEL+K++RIQ+LRC+ +Y + TRL
Sbjct: 185 RKAENEERIVTAAEQLSTDYCYAFTVKKPGLVPPVKELQKVLRIQALRCHVIYCQNGTRL 364
Query: 695 SVVPIHASRSQALR 736
+V+P+ ASRSQALR
Sbjct: 365 NVIPVLASRSQALR 406
Score = 64.3 bits (134), Expect(3) = 8e-039
Identities = 25/44 (56%), Positives = 32/44 (72%)
Frame = +2 / +1
Query: 392 TSNNSKITFALDQNHQSHIEYRWGGEGLRKYLVKWATSVVERKG 523
T N F +D + SHIEYRWGGEGLRK LV+WA+SV +++G
Sbjct: 61 TPNPEDGPFVVDFYYHSHIEYRWGGEGLRKTLVRWASSVSDKEG 192
Score = 43.2 bits (88), Expect(3) = 8e-039
Identities = 15/23 (65%), Positives = 20/23 (86%)
Frame = +2 / +1
Query: 323 PTDFDAFICNSGSNIYYPSHSRE 391
P+DFDAFICNSGS++YY + + E
Sbjct: 7 PSDFDAFICNSGSDLYYSTPNPE 75
>gb|BU885401.1|BU885401 R030G01 Populus root cDNA library Populus tremula x Populus
tremuloides cDNA 5 prime, mRNA sequence
Length = 589
Score = 104 bits (221), Expect(2) = 1e-032
Identities = 45/83 (54%), Positives = 55/83 (66%)
Frame = +2 / +3
Query: 143 RKNIIVISVDSVNKEGLVRIIRNAIEVIHKENMSGSTGFVLSTSLTISEIHSLLLSGGML 322
RK I V++VD L IR E + KE + GS GF+LSTSL ISEI S L SGG
Sbjct: 234 RKQIFVVAVDFDTFASLAEAIRKIFEAVEKERVEGSIGFILSTSLVISEICSFLASGGFS 413
Query: 323 PTDFDAFICNSGSNIYYPSHSRE 391
P+DFDAFICNSGS++YY + + E
Sbjct: 414 PSDFDAFICNSGSDLYYSTPNPE 482
Score = 56.1 bits (116), Expect(2) = 1e-032
Identities = 21/40 (52%), Positives = 27/40 (67%)
Frame = +2 / +3
Query: 392 TSNNSKITFALDQNHQSHIEYRWGGEGLRKYLVKWATSVV 511
T N F +D + HIEYRWGGEGLRK L +W++SV+
Sbjct: 468 TPNPEDGPFVIDFYYHPHIEYRWGGEGLRKTLFRWSSSVI 587
>gb|DT483569.1|DT483569 WS02522.B21_P13 PT-MB-N-A-15 Populus trichocarpa cDNA clone
WS02522_P13 3', mRNA sequence
Length = 813
Score = 102 bits (218), Expect(3) = 5e-028
Identities = 42/92 (45%), Positives = 64/92 (69%)
Frame = +2 / +1
Query: 461 GGEGLRKYLVKWATSVVERKGRTERQIIFEDPEHSSAYCLAFRVVNPNHLPPLKELRKLM 640
G EGLRK L W +S+++ K ++I+ + S+ YC AF V P +PP+KEL+K++
Sbjct: 121 GAEGLRKTLFLWPSSLIDNKADDAQRIVSSP*QLSTDYCYAFTVKKPVSVPPVKELQKVL 300
Query: 641 RIQSLRCNALYNHSATRLSVVPIHASRSQALR 736
IQ+LRC+A+Y + T ++V+P+ ASRSQALR
Sbjct: 301 TIQALRCHAIY*RNGTTINVIPVLASRSQALR 396
Score = 33.6 bits (67), Expect(3) = 5e-028
Identities = 11/15 (73%), Positives = 13/15 (86%)
Frame = +2 / +1
Query: 329 DFDAFICNSGSNIYY 373
DFDAFICN G ++YY
Sbjct: 1 DFDAFICNLGRDLYY 45
Score = 25.8 bits (50), Expect(3) = 5e-028
Identities = 9/18 (50%), Positives = 12/18 (66%)
Frame = +2 / +3
Query: 410 ITFALDQNHQSHIEYRWG 463
+ F +D + SHIEYR G
Sbjct: 69 VPFVIDFYYHSHIEYRCG 122
>gb|DT488170.1|DT488170 WS02535.B21_L05 PT-MB-N-A-15 Populus trichocarpa cDNA clone
WS02535_L05 3', mRNA sequence
Length = 901
Score = 101 bits (215), Expect = 6e-021
Identities = 41/81 (50%), Positives = 59/81 (72%)
Frame = +2 / -2
Query: 494 WATSVVERKGRTERQIIFEDPEHSSAYCLAFRVVNPNHLPPLKELRKLMRIQSLRCNALY 673
WA SV ++K E++I+ + S+ YC AF V P +PP+KEL+K +RIQ+LRC+A+Y
Sbjct: 900 WAFSVSDKKAENEKRIVTAAEQLSTDYCYAFTVKKPGLVPPVKELQKALRIQALRCHAIY 721
Query: 674 NHSATRLSVVPIHASRSQALR 736
+ TRL+V+P+ ASRSQALR
Sbjct: 720 CQNGTRLNVIPVLASRSQALR 658
Database: Populus_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:49 PM
Number of letters in database: 203,408,664
Number of sequences in database: 369,679
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 364,077,037
Number of Sequences: 369679
Number of extensions: 5087984
Number of successful extensions: 40460
Number of sequences better than 1.0e-020: 7
length of database: 67,802,888
effective HSP length: 52
effective length of database: 48,579,580
effective search space used: 9375858940
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)