TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2161272.2.1
(747 letters)
Database: Populus_nucl_with_EST.fasta
369,679 sequences; 203,408,664 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CX659519.1|CX659519 PO01031F07 Poplar SC cDNA library Po... 58 6e-027
gb|DT518564.1|DT518564 WS02438.B21_I18 PTxD-ICC-N-A-14 Popu... 57 2e-025
gb|CV260564.1|CV260564 WS02014.B21_G14 PTxN-IB-N-A-11 Popul... 57 2e-025
gb|DT512363.1|DT512363 WS02419.B21_B06 PTxD-ICC-N-A-14 Popu... 57 2e-025
gb|DT517819.1|DT517819 WS02435.B21_G10 PTxD-ICC-N-A-14 Popu... 57 2e-025
gb|CV257933.1|CV257933 WS0248.B21_A20 PTxD-ICC-N-A-14 Popul... 57 2e-025
gb|CV258295.1|CV258295 WS0249.B21_B24 PTxD-ICC-N-A-14 Popul... 57 2e-025
gb|CV259723.1|CV259723 WS02012.B21_B17 PTxN-IB-N-A-11 Popul... 57 2e-025
gb|CV270280.1|CV270280 WS0151.B21_J04 PTxN-IB-A-6 Populus t... 57 2e-024
gb|BI138921.1|BI138921 F119P23Y Populus flower cDNA library... 57 8e-024
gb|BU815039.1|BU815039 N036D09 Populus bark cDNA library Po... 56 2e-023
>gb|CX659519.1|CX659519 PO01031F07 Poplar SC cDNA library Populus alba x Populus tremula
var. glandulosa cDNA clone PO01031F07 5', mRNA sequence
Length = 633
Score = 57.9 bits (120), Expect(3) = 6e-027
Identities = 20/40 (50%), Positives = 30/40 (75%)
Frame = +3 / +3
Query: 51 YHFDLSGKAFGSLAKPGLNDKLRHCGIMDVEFRRVRCKYP 170
+HFDLSG AFG++A G D+LR+ G++ +E +RV C +P
Sbjct: 87 FHFDLSGTAFGAMAISGKEDQLRNAGVLQIEHQRVPCNWP 206
Score = 54.7 bits (113), Expect(3) = 6e-027
Identities = 20/47 (42%), Positives = 27/47 (57%)
Frame = +3 / +3
Query: 291 SAEWKPMKLSWGAIWRMDTAKALKGPFSIRLTSESGKKVIAKDVIPP 431
S W PM+ SWGA+W++D L+ P SI+LTS K I + P
Sbjct: 327 SDSWVPMQKSWGAVWKLDAGSLLRAPLSIKLTSLESGKTIVASGVIP 467
Score = 46.0 bits (94), Expect(3) = 6e-027
Identities = 15/42 (35%), Positives = 28/42 (66%)
Frame = +3 / +3
Query: 174 GQKIVFHIEKGCNPNYLAVLVKFVADDGDIVLMEIQDKLSAE 299
G+ + FH++ G NP Y A LV++ DGD+ +E++ L+++
Sbjct: 207 GKTVTFHVDSGSNPYYFATLVEYEDGDGDLKSVELKQALNSD 332
>gb|DT518564.1|DT518564 WS02438.B21_I18 PTxD-ICC-N-A-14 Populus trichocarpa x Populus
deltoides cDNA clone WS02438_I18 3', mRNA sequence
Length = 905
Score = 56.5 bits (117), Expect(3) = 2e-025
Identities = 19/40 (47%), Positives = 30/40 (75%)
Frame = +3 / -1
Query: 51 YHFDLSGKAFGSLAKPGLNDKLRHCGIMDVEFRRVRCKYP 170
+HFDLSG AFG++A G D+LR+ G++ ++ +RV C +P
Sbjct: 587 FHFDLSGTAFGAMAISGKEDQLRNAGVLQIQHQRVPCNWP 468
Score = 54.7 bits (113), Expect(3) = 2e-025
Identities = 20/47 (42%), Positives = 27/47 (57%)
Frame = +3 / -1
Query: 291 SAEWKPMKLSWGAIWRMDTAKALKGPFSIRLTSESGKKVIAKDVIPP 431
S W PM+ SWGA+W++D L+ P SI+LTS K I + P
Sbjct: 347 SDSWVPMQKSWGAVWKLDAGSLLRAPLSIKLTSLESGKTIVASGVIP 207
Score = 42.3 bits (86), Expect(3) = 2e-025
Identities = 13/42 (30%), Positives = 27/42 (64%)
Frame = +3 / -1
Query: 174 GQKIVFHIEKGCNPNYLAVLVKFVADDGDIVLMEIQDKLSAE 299
G+ + FH++ G NP Y A +V++ DG++ +E++ L ++
Sbjct: 467 GKTVTFHVDSGSNPYYFATVVEYEDGDGELKSVELKQALDSD 342
>gb|CV260564.1|CV260564 WS02014.B21_G14 PTxN-IB-N-A-11 Populus trichocarpa x Populus nigra
cDNA clone WS02014_G14 3', mRNA sequence
Length = 893
Score = 56.5 bits (117), Expect(3) = 2e-025
Identities = 19/40 (47%), Positives = 30/40 (75%)
Frame = +3 / -3
Query: 51 YHFDLSGKAFGSLAKPGLNDKLRHCGIMDVEFRRVRCKYP 170
+HFDLSG AFG++A G D+LR+ G++ ++ +RV C +P
Sbjct: 639 FHFDLSGTAFGAMAISGKEDQLRNAGVLQIQHQRVPCNWP 520
Score = 54.7 bits (113), Expect(3) = 2e-025
Identities = 20/47 (42%), Positives = 27/47 (57%)
Frame = +3 / -3
Query: 291 SAEWKPMKLSWGAIWRMDTAKALKGPFSIRLTSESGKKVIAKDVIPP 431
S W PM+ SWGA+W++D L+ P SI+LTS K I + P
Sbjct: 399 SDSWVPMQKSWGAVWKLDAGSLLRAPLSIKLTSLESGKTIVASGVIP 259
Score = 42.3 bits (86), Expect(3) = 2e-025
Identities = 13/42 (30%), Positives = 27/42 (64%)
Frame = +3 / -3
Query: 174 GQKIVFHIEKGCNPNYLAVLVKFVADDGDIVLMEIQDKLSAE 299
G+ + FH++ G NP Y A +V++ DG++ +E++ L ++
Sbjct: 519 GKTVTFHVDSGSNPYYFATVVEYEDGDGELKSVELKQALDSD 394
>gb|DT512363.1|DT512363 WS02419.B21_B06 PTxD-ICC-N-A-14 Populus trichocarpa x Populus
deltoides cDNA clone WS02419_B06 3', mRNA sequence
Length = 837
Score = 56.5 bits (117), Expect(3) = 2e-025
Identities = 19/40 (47%), Positives = 30/40 (75%)
Frame = +3 / -2
Query: 51 YHFDLSGKAFGSLAKPGLNDKLRHCGIMDVEFRRVRCKYP 170
+HFDLSG AFG++A G D+LR+ G++ ++ +RV C +P
Sbjct: 587 FHFDLSGTAFGAMAISGKEDQLRNAGVLQIQHQRVPCNWP 468
Score = 54.7 bits (113), Expect(3) = 2e-025
Identities = 20/47 (42%), Positives = 27/47 (57%)
Frame = +3 / -2
Query: 291 SAEWKPMKLSWGAIWRMDTAKALKGPFSIRLTSESGKKVIAKDVIPP 431
S W PM+ SWGA+W++D L+ P SI+LTS K I + P
Sbjct: 347 SDSWVPMQKSWGAVWKLDAGSLLRAPLSIKLTSLESGKTIVASGVIP 207
Score = 42.3 bits (86), Expect(3) = 2e-025
Identities = 13/42 (30%), Positives = 27/42 (64%)
Frame = +3 / -2
Query: 174 GQKIVFHIEKGCNPNYLAVLVKFVADDGDIVLMEIQDKLSAE 299
G+ + FH++ G NP Y A +V++ DG++ +E++ L ++
Sbjct: 467 GKTVTFHVDSGSNPYYFATVVEYEDGDGELKSVELKQALDSD 342
>gb|DT517819.1|DT517819 WS02435.B21_G10 PTxD-ICC-N-A-14 Populus trichocarpa x Populus
deltoides cDNA clone WS02435_G10 3', mRNA sequence
Length = 831
Score = 56.5 bits (117), Expect(3) = 2e-025
Identities = 19/40 (47%), Positives = 30/40 (75%)
Frame = +3 / -1
Query: 51 YHFDLSGKAFGSLAKPGLNDKLRHCGIMDVEFRRVRCKYP 170
+HFDLSG AFG++A G D+LR+ G++ ++ +RV C +P
Sbjct: 567 FHFDLSGTAFGAMAISGKEDQLRNAGVLQIQHQRVPCNWP 448
Score = 54.7 bits (113), Expect(3) = 2e-025
Identities = 20/47 (42%), Positives = 27/47 (57%)
Frame = +3 / -1
Query: 291 SAEWKPMKLSWGAIWRMDTAKALKGPFSIRLTSESGKKVIAKDVIPP 431
S W PM+ SWGA+W++D L+ P SI+LTS K I + P
Sbjct: 327 SDSWVPMQKSWGAVWKLDAGSLLRAPLSIKLTSLESGKTIVASGVIP 187
Score = 42.3 bits (86), Expect(3) = 2e-025
Identities = 13/42 (30%), Positives = 27/42 (64%)
Frame = +3 / -1
Query: 174 GQKIVFHIEKGCNPNYLAVLVKFVADDGDIVLMEIQDKLSAE 299
G+ + FH++ G NP Y A +V++ DG++ +E++ L ++
Sbjct: 447 GKTVTFHVDSGSNPYYFATVVEYEDGDGELKSVELKQALDSD 322
>gb|CV257933.1|CV257933 WS0248.B21_A20 PTxD-ICC-N-A-14 Populus trichocarpa x Populus
deltoides cDNA clone WS0248_A20 3', mRNA sequence
Length = 808
Score = 56.5 bits (117), Expect(3) = 2e-025
Identities = 19/40 (47%), Positives = 30/40 (75%)
Frame = +3 / -3
Query: 51 YHFDLSGKAFGSLAKPGLNDKLRHCGIMDVEFRRVRCKYP 170
+HFDLSG AFG++A G D+LR+ G++ ++ +RV C +P
Sbjct: 566 FHFDLSGTAFGAMAISGKEDQLRNAGVLQIQHQRVPCNWP 447
Score = 54.7 bits (113), Expect(3) = 2e-025
Identities = 20/47 (42%), Positives = 27/47 (57%)
Frame = +3 / -3
Query: 291 SAEWKPMKLSWGAIWRMDTAKALKGPFSIRLTSESGKKVIAKDVIPP 431
S W PM+ SWGA+W++D L+ P SI+LTS K I + P
Sbjct: 326 SDSWVPMQKSWGAVWKLDAGSLLRAPLSIKLTSLESGKTIVASGVIP 186
Score = 42.3 bits (86), Expect(3) = 2e-025
Identities = 13/42 (30%), Positives = 27/42 (64%)
Frame = +3 / -3
Query: 174 GQKIVFHIEKGCNPNYLAVLVKFVADDGDIVLMEIQDKLSAE 299
G+ + FH++ G NP Y A +V++ DG++ +E++ L ++
Sbjct: 446 GKTVTFHVDSGSNPYYFATVVEYEDGDGELKSVELKQALDSD 321
>gb|CV258295.1|CV258295 WS0249.B21_B24 PTxD-ICC-N-A-14 Populus trichocarpa x Populus
deltoides cDNA clone WS0249_B24 3', mRNA sequence
Length = 741
Score = 56.5 bits (117), Expect(3) = 2e-025
Identities = 19/40 (47%), Positives = 30/40 (75%)
Frame = +3 / -1
Query: 51 YHFDLSGKAFGSLAKPGLNDKLRHCGIMDVEFRRVRCKYP 170
+HFDLSG AFG++A G D+LR+ G++ ++ +RV C +P
Sbjct: 663 FHFDLSGTAFGAMAISGKEDQLRNAGVLQIQHQRVPCNWP 544
Score = 54.7 bits (113), Expect(3) = 2e-025
Identities = 20/47 (42%), Positives = 27/47 (57%)
Frame = +3 / -1
Query: 291 SAEWKPMKLSWGAIWRMDTAKALKGPFSIRLTSESGKKVIAKDVIPP 431
S W PM+ SWGA+W++D L+ P SI+LTS K I + P
Sbjct: 423 SDSWVPMQKSWGAVWKLDAGSLLRAPLSIKLTSLESGKTIVASGVIP 283
Score = 42.3 bits (86), Expect(3) = 2e-025
Identities = 13/42 (30%), Positives = 27/42 (64%)
Frame = +3 / -1
Query: 174 GQKIVFHIEKGCNPNYLAVLVKFVADDGDIVLMEIQDKLSAE 299
G+ + FH++ G NP Y A +V++ DG++ +E++ L ++
Sbjct: 543 GKTVTFHVDSGSNPYYFATVVEYEDGDGELKSVELKQALDSD 418
>gb|CV259723.1|CV259723 WS02012.B21_B17 PTxN-IB-N-A-11 Populus trichocarpa x Populus nigra
cDNA clone WS02012_B17 3', mRNA sequence
Length = 724
Score = 56.5 bits (117), Expect(3) = 2e-025
Identities = 19/40 (47%), Positives = 30/40 (75%)
Frame = +3 / -2
Query: 51 YHFDLSGKAFGSLAKPGLNDKLRHCGIMDVEFRRVRCKYP 170
+HFDLSG AFG++A G D+LR+ G++ ++ +RV C +P
Sbjct: 591 FHFDLSGTAFGAMAISGKEDQLRNAGVLQIQHQRVPCNWP 472
Score = 54.7 bits (113), Expect(3) = 2e-025
Identities = 20/47 (42%), Positives = 27/47 (57%)
Frame = +3 / -2
Query: 291 SAEWKPMKLSWGAIWRMDTAKALKGPFSIRLTSESGKKVIAKDVIPP 431
S W PM+ SWGA+W++D L+ P SI+LTS K I + P
Sbjct: 351 SDSWVPMQKSWGAVWKLDAGSLLRAPLSIKLTSLESGKTIVASGVIP 211
Score = 42.3 bits (86), Expect(3) = 2e-025
Identities = 13/42 (30%), Positives = 27/42 (64%)
Frame = +3 / -2
Query: 174 GQKIVFHIEKGCNPNYLAVLVKFVADDGDIVLMEIQDKLSAE 299
G+ + FH++ G NP Y A +V++ DG++ +E++ L ++
Sbjct: 471 GKTVTFHVDSGSNPYYFATVVEYEDGDGELKSVELKQALDSD 346
>gb|CV270280.1|CV270280 WS0151.B21_J04 PTxN-IB-A-6 Populus trichocarpa x Populus nigra cDNA
clone WS0151_J04 3', mRNA sequence
Length = 913
Score = 57.0 bits (118), Expect(3) = 2e-024
Identities = 21/54 (38%), Positives = 31/54 (57%)
Frame = +3 / -2
Query: 174 GQKIVFHIEKGCNPNYLAVLVKFVADDGDIVLMEIQDKLSAEWKPMKLSWGAIW 335
G+ I FH+ +G +L++LV+F DGD+ M I++ EW M WGA W
Sbjct: 681 GKNIAFHVNEGSTDYWLSILVEFEDGDGDVGSMHIREAGGTEWLEMNHVWGATW 520
Score = 52.8 bits (109), Expect(3) = 2e-024
Identities = 22/39 (56%), Positives = 27/39 (69%)
Frame = +3 / -2
Query: 54 HFDLSGKAFGSLAKPGLNDKLRHCGIMDVEFRRVRCKYP 170
HFDLSG AFG +A G N +LR+ G + V +RR CKYP
Sbjct: 798 HFDLSGAAFGRMAISGENGQLRNRGEIPVIYRRTPCKYP 682
Score = 40.5 bits (82), Expect(3) = 2e-024
Identities = 14/31 (45%), Positives = 21/31 (67%)
Frame = +3 / -2
Query: 384 TSESGKKVIAKDVIPPNWRPDAVYTSNVQFY 476
T +G+ + A++VIP NW P A YTS + F+
Sbjct: 471 TLTTGRTLSAREVIPRNWAPKATYTSRLNFF 379
>gb|BI138921.1|BI138921 F119P23Y Populus flower cDNA library Populus trichocarpa cDNA, mRNA
sequence
Length = 493
Score = 57.0 bits (118), Expect(3) = 8e-024
Identities = 21/54 (38%), Positives = 31/54 (57%)
Frame = +3 / +1
Query: 174 GQKIVFHIEKGCNPNYLAVLVKFVADDGDIVLMEIQDKLSAEWKPMKLSWGAIW 335
G+ I FH+ +G +L++LV+F DGD+ M I++ EW M WGA W
Sbjct: 184 GKNIAFHVNEGSTDYWLSILVEFEDGDGDVGSMHIREAGGTEWLEMNHVWGATW 345
Score = 50.6 bits (104), Expect(3) = 8e-024
Identities = 21/39 (53%), Positives = 26/39 (66%)
Frame = +3 / +1
Query: 54 HFDLSGKAFGSLAKPGLNDKLRHCGIMDVEFRRVRCKYP 170
HFDLSG AFG +A G N +LR+ G + V +RR C YP
Sbjct: 67 HFDLSGAAFGRMAISGENGQLRNRGEIPVIYRRTPCNYP 183
Score = 40.5 bits (82), Expect(3) = 8e-024
Identities = 14/31 (45%), Positives = 21/31 (67%)
Frame = +3 / +1
Query: 384 TSESGKKVIAKDVIPPNWRPDAVYTSNVQFY 476
T +G+ + A++VIP NW P A YTS + F+
Sbjct: 394 TLTTGRTLSAREVIPRNWTPKATYTSRLNFF 486
>gb|BU815039.1|BU815039 N036D09 Populus bark cDNA library Populus tremula x Populus
tremuloides cDNA 5 prime, mRNA sequence
Length = 767
Score = 56.1 bits (116), Expect(3) = 2e-023
Identities = 21/54 (38%), Positives = 31/54 (57%)
Frame = +3 / +1
Query: 174 GQKIVFHIEKGCNPNYLAVLVKFVADDGDIVLMEIQDKLSAEWKPMKLSWGAIW 335
G+ I FH+ +G +L++LV+F DGD+ M I++ EW M WGA W
Sbjct: 337 GKNIAFHVNEGSTDYWLSLLVEFEDGDGDVGSMHIREAGGTEWLEMNHVWGATW 498
Score = 53.3 bits (110), Expect(3) = 2e-023
Identities = 22/39 (56%), Positives = 27/39 (69%)
Frame = +3 / +1
Query: 54 HFDLSGKAFGSLAKPGLNDKLRHCGIMDVEFRRVRCKYP 170
HFDLSG AFG +A G N +LR+ G + V +RR CKYP
Sbjct: 220 HFDLSGAAFGRMAISGENGQLRNRGELPVIYRRTPCKYP 336
Score = 37.3 bits (75), Expect(3) = 2e-023
Identities = 13/31 (41%), Positives = 20/31 (64%)
Frame = +3 / +1
Query: 384 TSESGKKVIAKDVIPPNWRPDAVYTSNVQFY 476
T + + + A++VIP NW P A YTS + F+
Sbjct: 547 TLSTRRTLSAREVIPRNWAPKATYTSRLNFF 639
Database: Populus_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:49 PM
Number of letters in database: 203,408,664
Number of sequences in database: 369,679
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 387,764,758
Number of Sequences: 369679
Number of extensions: 5826987
Number of successful extensions: 53856
Number of sequences better than 1.0e-020: 11
length of database: 67,802,888
effective HSP length: 52
effective length of database: 48,579,580
effective search space used: 9521597680
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)