TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 1804898.2.1
(739 letters)
Database: Populus_nucl_with_EST.fasta
369,679 sequences; 203,408,664 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|DN488662.1|DN488662 S021B06.3pR Populus imbibed seed cDN... 79 4e-028
gb|CK096481.1|CK096481 UB15CPH11.3pR Populus active cambium... 73 6e-027
gb|CV280707.1|CV280707 WS0137.B21_G07 PTxD-IL-FL-A-4 Populu... 70 5e-024
gb|CV280517.1|CV280517 WS0136.B21_J20 PTxD-IL-FL-A-4 Populu... 70 5e-024
gb|DT476355.1|DT476355 WS01229.B21_E11 PT-GT-FL-A-3 Populus... 67 5e-024
gb|DT504273.1|DT504273 WS01312.B21_L07 PTxD-IL-FL-A-4 Popul... 70 5e-024
gb|CV235235.1|CV235235 WS01218.B21_E23 PT-GT-FL-A-3 Populus... 67 1e-023
gb|AJ772970.1|AJ772970 AJ772970 Populus euphratica leaf 3-6... 93 2e-023
gb|CA932779.1|CA932779 MTU5CS.P17.C10 Aspen stem cDNA Libra... 69 1e-022
gb|CV234783.1|CV234783 WS01216.B21_E17 PT-GT-FL-A-3 Populus... 67 1e-020
>gb|DN488662.1|DN488662 S021B06.3pR Populus imbibed seed cDNA library Populus tremula cDNA
clone S021B06 3', mRNA sequence
Length = 568
Score = 79.4 bits (167), Expect(3) = 4e-028
Identities = 26/43 (60%), Positives = 37/43 (86%)
Frame = +2 / -3
Query: 104 GTAGLSFHGGDMFEHIPSADALMLKWILHDWDEDKCIKIMERC 232
G+ L + GGDMFE IP ADA++LKWILHDW+E++C+KI+++C
Sbjct: 455 GSENLKYLGGDMFEAIPQADAILLKWILHDWNEEECVKILKQC 327
Score = 55.1 bits (114), Expect(3) = 4e-028
Identities = 23/48 (47%), Positives = 31/48 (64%)
Frame = +2 / -3
Query: 350 DLHILSFVNGAEREEHEWRRIFLAAGFRDYKITHTRGIPSIIEVFP** 493
D+ ++ V G ER E EW ++F AGF YKI G+ S+IEV+P**
Sbjct: 221 DMLMMILVTGKERNEKEWAKLFTDAGFSHYKINPVLGLRSLIEVYP** 78
Score = 28.1 bits (55), Expect(3) = 4e-028
Identities = 10/11 (90%), Positives = 10/11 (90%)
Frame = +2 / -3
Query: 59 CTVMDLPHVVA 91
CTV DLPHVVA
Sbjct: 497 CTVFDLPHVVA 465
>gb|CK096481.1|CK096481 UB15CPH11.3pR Populus active cambium cDNA library Populus tremula
cDNA clone UB15CPH11 3', mRNA sequence
Length = 882
Score = 73.5 bits (154), Expect(4) = 6e-027
Identities = 23/43 (53%), Positives = 34/43 (79%)
Frame = +2 / -2
Query: 104 GTAGLSFHGGDMFEHIPSADALMLKWILHDWDEDKCIKIMERC 232
G L + GGDMF+ +P A+A++LKWILHDW ++ C+KI++RC
Sbjct: 566 GCKNLKYVGGDMFQGVPPAEAILLKWILHDWSDEDCVKILKRC 438
Score = 51.9 bits (107), Expect(4) = 6e-027
Identities = 21/50 (42%), Positives = 32/50 (64%)
Frame = +2 / -2
Query: 344 VLDLHILSFVNGAEREEHEWRRIFLAAGFRDYKITHTRGIPSIIEVFP** 493
+ DLH++ +G ER E EW ++F AGF +YKI ++IE++P**
Sbjct: 329 LFDLHMMVDADGMERNEKEWAKLFFDAGFLNYKIHPVLATRALIELYP** 180
Score = 27.6 bits (54), Expect(4) = 6e-027
Identities = 9/12 (75%), Positives = 11/12 (91%)
Frame = +2 / -2
Query: 59 CTVMDLPHVVAE 94
CTV DLPHVV++
Sbjct: 608 CTVFDLPHVVSD 573
Score = 22.6 bits (43), Expect(4) = 6e-027
Identities = 8/11 (72%), Positives = 9/11 (81%)
Frame = +1 / -1
Query: 262 GEGDNNRHGAR 294
GEGDN R+G R
Sbjct: 402 GEGDNYRYGDR 370
>gb|CV280707.1|CV280707 WS0137.B21_G07 PTxD-IL-FL-A-4 Populus trichocarpa x Populus
deltoides cDNA clone WS0137_G07 3', mRNA sequence
Length = 919
Score = 69.8 bits (146), Expect(3) = 5e-024
Identities = 22/43 (51%), Positives = 33/43 (76%)
Frame = +2 / -2
Query: 104 GTAGLSFHGGDMFEHIPSADALMLKWILHDWDEDKCIKIMERC 232
G L F GDMF+ +P A+A++LKWILH+W ++ C+KI++RC
Sbjct: 540 GCKNLKFVAGDMFQAVPPAEAILLKWILHNWSDEDCVKILKRC 412
Score = 50.1 bits (103), Expect(3) = 5e-024
Identities = 21/48 (43%), Positives = 30/48 (62%)
Frame = +2 / -2
Query: 350 DLHILSFVNGAEREEHEWRRIFLAAGFRDYKITHTRGIPSIIEVFP** 493
DL ++ G ER E EW ++F AGF +YKI G ++IE++P**
Sbjct: 297 DLQMMVVAGGMERNEKEWAKLFFDAGFLNYKIYPVLGPRALIELYP** 154
Score = 28.6 bits (56), Expect(3) = 5e-024
Identities = 9/12 (75%), Positives = 12/12 (100%)
Frame = +2 / -2
Query: 59 CTVMDLPHVVAE 94
CTV+DLPHVV++
Sbjct: 582 CTVLDLPHVVSD 547
>gb|CV280517.1|CV280517 WS0136.B21_J20 PTxD-IL-FL-A-4 Populus trichocarpa x Populus
deltoides cDNA clone WS0136_J20 3', mRNA sequence
Length = 893
Score = 69.8 bits (146), Expect(3) = 5e-024
Identities = 22/43 (51%), Positives = 33/43 (76%)
Frame = +2 / -2
Query: 104 GTAGLSFHGGDMFEHIPSADALMLKWILHDWDEDKCIKIMERC 232
G L F GDMF+ +P A+A++LKWILH+W ++ C+KI++RC
Sbjct: 532 GCKNLKFVAGDMFQAVPPAEAILLKWILHNWSDEDCVKILKRC 404
Score = 50.1 bits (103), Expect(3) = 5e-024
Identities = 21/48 (43%), Positives = 30/48 (62%)
Frame = +2 / -2
Query: 350 DLHILSFVNGAEREEHEWRRIFLAAGFRDYKITHTRGIPSIIEVFP** 493
DL ++ G ER E EW ++F AGF +YKI G ++IE++P**
Sbjct: 289 DLQMMVVAGGMERNEKEWAKLFFDAGFLNYKIYPVLGPRALIELYP** 146
Score = 28.6 bits (56), Expect(3) = 5e-024
Identities = 9/12 (75%), Positives = 12/12 (100%)
Frame = +2 / -2
Query: 59 CTVMDLPHVVAE 94
CTV+DLPHVV++
Sbjct: 574 CTVLDLPHVVSD 539
>gb|DT476355.1|DT476355 WS01229.B21_E11 PT-GT-FL-A-3 Populus trichocarpa cDNA clone
WS01229_E11 3', mRNA sequence
Length = 887
Score = 67.0 bits (140), Expect(3) = 5e-024
Identities = 20/43 (46%), Positives = 33/43 (76%)
Frame = +2 / -2
Query: 104 GTAGLSFHGGDMFEHIPSADALMLKWILHDWDEDKCIKIMERC 232
G L + GDMF+ +P A+A++LKW+LH+W ++ C+KI++RC
Sbjct: 607 GCKNLKYVAGDMFQAVPPAEAILLKWMLHNWSDEDCVKILKRC 479
Score = 52.8 bits (109), Expect(3) = 5e-024
Identities = 22/48 (45%), Positives = 31/48 (64%)
Frame = +2 / -2
Query: 350 DLHILSFVNGAEREEHEWRRIFLAAGFRDYKITHTRGIPSIIEVFP** 493
DL ++ V G ER E EW ++F AGF +YKI G ++IE++P**
Sbjct: 364 DLQMMVVVGGMERNEKEWAKLFFDAGFLNYKIHPVLGTRALIELYP** 221
Score = 28.6 bits (56), Expect(3) = 5e-024
Identities = 9/12 (75%), Positives = 12/12 (100%)
Frame = +2 / -2
Query: 59 CTVMDLPHVVAE 94
CTV+DLPHVV++
Sbjct: 649 CTVLDLPHVVSD 614
>gb|DT504273.1|DT504273 WS01312.B21_L07 PTxD-IL-FL-A-4 Populus trichocarpa x Populus
deltoides cDNA clone WS01312_L07 3', mRNA sequence
Length = 822
Score = 69.8 bits (146), Expect(3) = 5e-024
Identities = 22/43 (51%), Positives = 33/43 (76%)
Frame = +2 / -1
Query: 104 GTAGLSFHGGDMFEHIPSADALMLKWILHDWDEDKCIKIMERC 232
G L F GDMF+ +P A+A++LKWILH+W ++ C+KI++RC
Sbjct: 561 GCKNLKFVAGDMFQAVPPAEAILLKWILHNWSDEDCVKILKRC 433
Score = 50.1 bits (103), Expect(3) = 5e-024
Identities = 21/48 (43%), Positives = 30/48 (62%)
Frame = +2 / -1
Query: 350 DLHILSFVNGAEREEHEWRRIFLAAGFRDYKITHTRGIPSIIEVFP** 493
DL ++ G ER E EW ++F AGF +YKI G ++IE++P**
Sbjct: 318 DLQMMVVAGGMERNEKEWAKLFFDAGFLNYKIYPVLGPRALIELYP** 175
Score = 28.6 bits (56), Expect(3) = 5e-024
Identities = 9/12 (75%), Positives = 12/12 (100%)
Frame = +2 / -1
Query: 59 CTVMDLPHVVAE 94
CTV+DLPHVV++
Sbjct: 603 CTVLDLPHVVSD 568
>gb|CV235235.1|CV235235 WS01218.B21_E23 PT-GT-FL-A-3 Populus trichocarpa cDNA clone
WS01218_E23 3', mRNA sequence
Length = 714
Score = 67.0 bits (140), Expect(3) = 1e-023
Identities = 20/43 (46%), Positives = 33/43 (76%)
Frame = +2 / -1
Query: 104 GTAGLSFHGGDMFEHIPSADALMLKWILHDWDEDKCIKIMERC 232
G L + GDMF+ +P A+A++LKW+LH+W ++ C+KI++RC
Sbjct: 597 GCKNLKYVAGDMFQAVPPAEAILLKWMLHNWSDEDCVKILKRC 469
Score = 52.8 bits (109), Expect(3) = 1e-023
Identities = 22/48 (45%), Positives = 31/48 (64%)
Frame = +2 / -1
Query: 350 DLHILSFVNGAEREEHEWRRIFLAAGFRDYKITHTRGIPSIIEVFP** 493
DL ++ V G ER E EW ++F AGF +YKI G ++IE++P**
Sbjct: 354 DLQMMVVVGGMERNEKEWAKLFFDAGFLNYKIHPVLGTRALIELYP** 211
Score = 27.2 bits (53), Expect(3) = 1e-023
Identities = 9/10 (90%), Positives = 10/10 (100%)
Frame = +2 / -1
Query: 59 CTVMDLPHVV 88
CTV+DLPHVV
Sbjct: 639 CTVLDLPHVV 610
>gb|AJ772970.1|AJ772970 AJ772970 Populus euphratica leaf 3-6 months Populus euphratica cDNA
clone P0000200003B11F1, mRNA sequence
Length = 597
Score = 92.7 bits (196), Expect(3) = 2e-023
Identities = 31/58 (53%), Positives = 44/58 (75%)
Frame = +2 / +2
Query: 59 CTVMDLPHVVAEAPAGTAGLSFHGGDMFEHIPSADALMLKWILHDWDEDKCIKIMERC 232
CTV DLP VV + AG+ L + GGDMFE +P AD ++LKW LHDW+++ C+KI+++C
Sbjct: 227 CTVFDLPRVVFDLQAGSKNLKYVGGDMFEAVPPADVILLKWTLHDWNDEDCVKILKQC 400
Score = 35.0 bits (70), Expect(3) = 2e-023
Identities = 13/27 (48%), Positives = 17/27 (62%)
Frame = +2 / +2
Query: 350 DLHILSFVNGAEREEHEWRRIFLAAGF 430
DL ++ V G ER E EW ++F AGF
Sbjct: 512 DLEMMVTVTGLERNEMEWAKLFFDAGF 592
Score = 21.7 bits (41), Expect(3) = 2e-023
Identities = 7/10 (70%), Positives = 8/10 (80%)
Frame = +1 / +1
Query: 265 EGDNNRHGAR 294
EGDN+RH R
Sbjct: 439 EGDNHRHSER 468
>gb|CA932779.1|CA932779 MTU5CS.P17.C10 Aspen stem cDNA Library Populus tremuloides cDNA,
mRNA sequence
Length = 624
Score = 69.3 bits (145), Expect(3) = 1e-022
Identities = 21/43 (48%), Positives = 33/43 (76%)
Frame = +2 / -1
Query: 104 GTAGLSFHGGDMFEHIPSADALMLKWILHDWDEDKCIKIMERC 232
G L + GDMF+ +P A+A++LKW+LHDW ++ C+KI++RC
Sbjct: 564 GCKNLKYVAGDMFQGVPPAEAILLKWMLHDWSDEDCVKILKRC 436
Score = 48.7 bits (100), Expect(3) = 1e-022
Identities = 20/50 (40%), Positives = 31/50 (62%)
Frame = +2 / -1
Query: 344 VLDLHILSFVNGAEREEHEWRRIFLAAGFRDYKITHTRGIPSIIEVFP** 493
+ DL ++ +G ER E EW ++F AGF +YKI ++IE++P**
Sbjct: 327 LFDLEMMVAASGMERNEEEWAKLFFDAGFLNYKIHPVLDTRALIELYP** 178
Score = 25.8 bits (50), Expect(3) = 1e-022
Identities = 8/12 (66%), Positives = 11/12 (91%)
Frame = +2 / -1
Query: 59 CTVMDLPHVVAE 94
CTV +LPHVV++
Sbjct: 606 CTVFELPHVVSD 571
>gb|CV234783.1|CV234783 WS01216.B21_E17 PT-GT-FL-A-3 Populus trichocarpa cDNA clone
WS01216_E17 3', mRNA sequence
Length = 633
Score = 67.0 bits (140), Expect(2) = 1e-020
Identities = 20/43 (46%), Positives = 33/43 (76%)
Frame = +2 / -1
Query: 104 GTAGLSFHGGDMFEHIPSADALMLKWILHDWDEDKCIKIMERC 232
G L + GDMF+ +P A+A++LKW+LH+W ++ C+KI++RC
Sbjct: 597 GCKNLKYVAGDMFQAVPPAEAILLKWMLHNWSDEDCVKILKRC 469
Score = 52.8 bits (109), Expect(2) = 1e-020
Identities = 22/48 (45%), Positives = 31/48 (64%)
Frame = +2 / -1
Query: 350 DLHILSFVNGAEREEHEWRRIFLAAGFRDYKITHTRGIPSIIEVFP** 493
DL ++ V G ER E EW ++F AGF +YKI G ++IE++P**
Sbjct: 354 DLQMMVVVGGMERNEKEWAKLFFDAGFLNYKIHPVLGTRALIELYP** 211
Database: Populus_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:49 PM
Number of letters in database: 203,408,664
Number of sequences in database: 369,679
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 298,112,826
Number of Sequences: 369679
Number of extensions: 4082792
Number of successful extensions: 45452
Number of sequences better than 1.0e-020: 10
length of database: 67,802,888
effective HSP length: 52
effective length of database: 48,579,580
effective search space used: 9375858940
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)