TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 131572.2.3
(1521 letters)
Database: Populus_nucl_with_EST.fasta
369,679 sequences; 203,408,664 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|DT476355.1|DT476355 WS01229.B21_E11 PT-GT-FL-A-3 Populus... 64 9e-024
gb|CV280707.1|CV280707 WS0137.B21_G07 PTxD-IL-FL-A-4 Populu... 65 2e-023
gb|CV280517.1|CV280517 WS0136.B21_J20 PTxD-IL-FL-A-4 Populu... 65 2e-023
gb|CK096481.1|CK096481 UB15CPH11.3pR Populus active cambium... 62 1e-022
gb|CV235235.1|CV235235 WS01218.B21_E23 PT-GT-FL-A-3 Populus... 64 3e-022
gb|DT504273.1|DT504273 WS01312.B21_L07 PTxD-IL-FL-A-4 Popul... 65 5e-022
gb|DT503437.1|DT503437 WS0136.BR_J20 PTxD-IL-FL-A-4 Populus... 64 2e-021
gb|DT503659.1|DT503659 WS0137.BR_G07 PTxD-IL-FL-A-4 Populus... 64 3e-021
gb|CA932779.1|CA932779 MTU5CS.P17.C10 Aspen stem cDNA Libra... 61 6e-021
gb|AJ772970.1|AJ772970 AJ772970 Populus euphratica leaf 3-6... 78 9e-021
>gb|DT476355.1|DT476355 WS01229.B21_E11 PT-GT-FL-A-3 Populus trichocarpa cDNA clone
WS01229_E11 3', mRNA sequence
Length = 887
Score = 64.3 bits (134), Expect(3) = 9e-024
Identities = 26/54 (48%), Positives = 34/54 (62%)
Frame = -1 / -2
Query: 510 LETHLLMDIGMMTMTKGRQRDEKEWSELFTKAGFSEYKILKEFGARVAFEVYP* 349
+ET L D+ MM + G +R+EKEW++LF AGF YKI G R E+YP*
Sbjct: 385 IETQLFFDLQMMVVVGGMERNEKEWAKLFFDAGFLNYKIHPVLGTRALIELYP* 224
Score = 51.5 bits (106), Expect(3) = 9e-024
Identities = 19/46 (41%), Positives = 29/46 (63%)
Frame = -1 / -2
Query: 705 INYVEGDMFSFIPPAQTXXXXXXXXXLTDEECVKLLAQCRKAIPSR 568
+ YV GDMF +PPA+ +DE+CVK+L +C++AI S+
Sbjct: 595 LKYVAGDMFQAVPPAEAILLKWMLHNWSDEDCVKILKRCKQAIMSK 458
Score = 33.1 bits (66), Expect(3) = 9e-024
Identities = 11/23 (47%), Positives = 17/23 (73%)
Frame = -1 / -2
Query: 798 ARAIVKAFPHIKCTVLAPPKIIA 730
A+ I +AFPH+ CTVL P +++
Sbjct: 685 AKGITEAFPHMDCTVLDLPHVVS 617
>gb|CV280707.1|CV280707 WS0137.B21_G07 PTxD-IL-FL-A-4 Populus trichocarpa x Populus
deltoides cDNA clone WS0137_G07 3', mRNA sequence
Length = 919
Score = 64.8 bits (135), Expect(3) = 2e-023
Identities = 26/54 (48%), Positives = 34/54 (62%)
Frame = -1 / -2
Query: 510 LETHLLMDIGMMTMTKGRQRDEKEWSELFTKAGFSEYKILKEFGARVAFEVYP* 349
+ET L D+ MM + G +R+EKEW++LF AGF YKI G R E+YP*
Sbjct: 318 IETQLFFDLQMMVVAGGMERNEKEWAKLFFDAGFLNYKIYPVLGPRALIELYP* 157
Score = 49.6 bits (102), Expect(3) = 2e-023
Identities = 18/46 (39%), Positives = 29/46 (63%)
Frame = -1 / -2
Query: 705 INYVEGDMFSFIPPAQTXXXXXXXXXLTDEECVKLLAQCRKAIPSR 568
+ +V GDMF +PPA+ +DE+CVK+L +C++AI S+
Sbjct: 528 LKFVAGDMFQAVPPAEAILLKWILHNWSDEDCVKILKRCKQAIMSK 391
Score = 33.1 bits (66), Expect(3) = 2e-023
Identities = 11/23 (47%), Positives = 17/23 (73%)
Frame = -1 / -2
Query: 798 ARAIVKAFPHIKCTVLAPPKIIA 730
A+ I +AFPH+ CTVL P +++
Sbjct: 618 AKGITEAFPHMDCTVLDLPHVVS 550
>gb|CV280517.1|CV280517 WS0136.B21_J20 PTxD-IL-FL-A-4 Populus trichocarpa x Populus
deltoides cDNA clone WS0136_J20 3', mRNA sequence
Length = 893
Score = 64.8 bits (135), Expect(3) = 2e-023
Identities = 26/54 (48%), Positives = 34/54 (62%)
Frame = -1 / -2
Query: 510 LETHLLMDIGMMTMTKGRQRDEKEWSELFTKAGFSEYKILKEFGARVAFEVYP* 349
+ET L D+ MM + G +R+EKEW++LF AGF YKI G R E+YP*
Sbjct: 310 IETQLFFDLQMMVVAGGMERNEKEWAKLFFDAGFLNYKIYPVLGPRALIELYP* 149
Score = 49.6 bits (102), Expect(3) = 2e-023
Identities = 18/46 (39%), Positives = 29/46 (63%)
Frame = -1 / -2
Query: 705 INYVEGDMFSFIPPAQTXXXXXXXXXLTDEECVKLLAQCRKAIPSR 568
+ +V GDMF +PPA+ +DE+CVK+L +C++AI S+
Sbjct: 520 LKFVAGDMFQAVPPAEAILLKWILHNWSDEDCVKILKRCKQAIMSK 383
Score = 33.1 bits (66), Expect(3) = 2e-023
Identities = 11/23 (47%), Positives = 17/23 (73%)
Frame = -1 / -2
Query: 798 ARAIVKAFPHIKCTVLAPPKIIA 730
A+ I +AFPH+ CTVL P +++
Sbjct: 610 AKGITEAFPHMDCTVLDLPHVVS 542
>gb|CK096481.1|CK096481 UB15CPH11.3pR Populus active cambium cDNA library Populus tremula
cDNA clone UB15CPH11 3', mRNA sequence
Length = 882
Score = 62.0 bits (129), Expect(3) = 1e-022
Identities = 26/54 (48%), Positives = 33/54 (61%)
Frame = -1 / -2
Query: 510 LETHLLMDIGMMTMTKGRQRDEKEWSELFTKAGFSEYKILKEFGARVAFEVYP* 349
+ET LL D+ MM G +R+EKEW++LF AGF YKI R E+YP*
Sbjct: 344 IETQLLFDLHMMVDADGMERNEKEWAKLFFDAGFLNYKIHPVLATRALIELYP* 183
Score = 51.9 bits (107), Expect(3) = 1e-022
Identities = 20/46 (43%), Positives = 29/46 (63%)
Frame = -1 / -2
Query: 705 INYVEGDMFSFIPPAQTXXXXXXXXXLTDEECVKLLAQCRKAIPSR 568
+ YV GDMF +PPA+ +DE+CVK+L +CR+AI S+
Sbjct: 554 LKYVGGDMFQGVPPAEAILLKWILHDWSDEDCVKILKRCRQAIMSK 417
Score = 31.3 bits (62), Expect(3) = 1e-022
Identities = 10/23 (43%), Positives = 16/23 (69%)
Frame = -1 / -2
Query: 798 ARAIVKAFPHIKCTVLAPPKIIA 730
A+ I +AFPH+ CTV P +++
Sbjct: 644 AKGIAEAFPHMDCTVFDLPHVVS 576
>gb|CV235235.1|CV235235 WS01218.B21_E23 PT-GT-FL-A-3 Populus trichocarpa cDNA clone
WS01218_E23 3', mRNA sequence
Length = 714
Score = 64.3 bits (134), Expect(3) = 3e-022
Identities = 26/54 (48%), Positives = 34/54 (62%)
Frame = -1 / -1
Query: 510 LETHLLMDIGMMTMTKGRQRDEKEWSELFTKAGFSEYKILKEFGARVAFEVYP* 349
+ET L D+ MM + G +R+EKEW++LF AGF YKI G R E+YP*
Sbjct: 375 IETQLFFDLQMMVVVGGMERNEKEWAKLFFDAGFLNYKIHPVLGTRALIELYP* 214
Score = 51.5 bits (106), Expect(3) = 3e-022
Identities = 19/46 (41%), Positives = 29/46 (63%)
Frame = -1 / -1
Query: 705 INYVEGDMFSFIPPAQTXXXXXXXXXLTDEECVKLLAQCRKAIPSR 568
+ YV GDMF +PPA+ +DE+CVK+L +C++AI S+
Sbjct: 585 LKYVAGDMFQAVPPAEAILLKWMLHNWSDEDCVKILKRCKQAIMSK 448
Score = 28.1 bits (55), Expect(3) = 3e-022
Identities = 10/22 (45%), Positives = 15/22 (68%)
Frame = -1 / -1
Query: 798 ARAIVKAFPHIKCTVLAPPKII 733
A+ I +AFP + CTVL P ++
Sbjct: 675 AKGITEAFPTMDCTVLDLPHVV 610
>gb|DT504273.1|DT504273 WS01312.B21_L07 PTxD-IL-FL-A-4 Populus trichocarpa x Populus
deltoides cDNA clone WS01312_L07 3', mRNA sequence
Length = 822
Score = 64.8 bits (135), Expect(3) = 5e-022
Identities = 26/54 (48%), Positives = 34/54 (62%)
Frame = -1 / -1
Query: 510 LETHLLMDIGMMTMTKGRQRDEKEWSELFTKAGFSEYKILKEFGARVAFEVYP* 349
+ET L D+ MM + G +R+EKEW++LF AGF YKI G R E+YP*
Sbjct: 339 IETQLFFDLQMMVVAGGMERNEKEWAKLFFDAGFLNYKIYPVLGPRALIELYP* 178
Score = 49.6 bits (102), Expect(3) = 5e-022
Identities = 18/46 (39%), Positives = 29/46 (63%)
Frame = -1 / -1
Query: 705 INYVEGDMFSFIPPAQTXXXXXXXXXLTDEECVKLLAQCRKAIPSR 568
+ +V GDMF +PPA+ +DE+CVK+L +C++AI S+
Sbjct: 549 LKFVAGDMFQAVPPAEAILLKWILHNWSDEDCVKILKRCKQAIMSK 412
Score = 28.6 bits (56), Expect(3) = 5e-022
Identities = 10/23 (43%), Positives = 16/23 (69%)
Frame = -1 / -1
Query: 798 ARAIVKAFPHIKCTVLAPPKIIA 730
A+ I +AFP + CTVL P +++
Sbjct: 639 AKGITEAFPPMDCTVLDLPHVVS 571
>gb|DT503437.1|DT503437 WS0136.BR_J20 PTxD-IL-FL-A-4 Populus trichocarpa x Populus deltoides
cDNA clone WS0136_J20 5', mRNA sequence
Length = 864
Score = 63.8 bits (133), Expect(3) = 2e-021
Identities = 28/77 (36%), Positives = 45/77 (58%)
Frame = -1 / +3
Query: 1416 SDAELLKAQADIWRLSLSYLTPLSLRCAVELGIPTAIYRHGGAASAAELVTALSLPSAKL 1237
S +ELL+AQ +W ++ ++L+CAV+LGIP I +HG + +ELV+AL + +K
Sbjct: 36 SASELLQAQTHVWNHIFYFINSMTLKCAVQLGIPDVIQKHGKPMTLSELVSALPIHPSKA 215
Query: 1236 PFXXXXXXXLAASGVFT 1186
+ L SG F+
Sbjct: 216 QYVHRLMRILVHSGFFS 266
Score = 49.6 bits (102), Expect(3) = 2e-021
Identities = 18/46 (39%), Positives = 29/46 (63%)
Frame = -1 / +3
Query: 705 INYVEGDMFSFIPPAQTXXXXXXXXXLTDEECVKLLAQCRKAIPSR 568
+ +V GDMF +PPA+ +DE+CVK+L +C++AI S+
Sbjct: 723 LKFVAGDMFQAVPPAEAILLKWILHNWSDEDCVKILKRCKQAIMSK 860
Score = 33.1 bits (66), Expect(3) = 2e-021
Identities = 11/23 (47%), Positives = 17/23 (73%)
Frame = -1 / +3
Query: 798 ARAIVKAFPHIKCTVLAPPKIIA 730
A+ I +AFPH+ CTVL P +++
Sbjct: 633 AKGITEAFPHMDCTVLDLPHVVS 701
>gb|DT503659.1|DT503659 WS0137.BR_G07 PTxD-IL-FL-A-4 Populus trichocarpa x Populus deltoides
cDNA clone WS0137_G07 5', mRNA sequence
Length = 905
Score = 63.8 bits (133), Expect(3) = 3e-021
Identities = 28/77 (36%), Positives = 45/77 (58%)
Frame = -1 / +3
Query: 1416 SDAELLKAQADIWRLSLSYLTPLSLRCAVELGIPTAIYRHGGAASAAELVTALSLPSAKL 1237
S +ELL+AQ +W ++ ++L+CAV+LGIP I +HG + +ELV+AL + +K
Sbjct: 39 SASELLQAQTHVWNHIFYFINSMTLKCAVQLGIPDVIQKHGKPMTLSELVSALPIHPSKA 218
Query: 1236 PFXXXXXXXLAASGVFT 1186
+ L SG F+
Sbjct: 219 QYVHRLMRILVHSGFFS 269
Score = 49.6 bits (102), Expect(3) = 3e-021
Identities = 18/46 (39%), Positives = 29/46 (63%)
Frame = -1 / +3
Query: 705 INYVEGDMFSFIPPAQTXXXXXXXXXLTDEECVKLLAQCRKAIPSR 568
+ +V GDMF +PPA+ +DE+CVK+L +C++AI S+
Sbjct: 726 LKFVAGDMFQAVPPAEAILLKWILHNWSDEDCVKILKRCKQAIMSK 863
Score = 33.1 bits (66), Expect(3) = 3e-021
Identities = 11/23 (47%), Positives = 17/23 (73%)
Frame = -1 / +3
Query: 798 ARAIVKAFPHIKCTVLAPPKIIA 730
A+ I +AFPH+ CTVL P +++
Sbjct: 636 AKGITEAFPHMDCTVLDLPHVVS 704
>gb|CA932779.1|CA932779 MTU5CS.P17.C10 Aspen stem cDNA Library Populus tremuloides cDNA,
mRNA sequence
Length = 624
Score = 61.1 bits (127), Expect(3) = 6e-021
Identities = 25/54 (46%), Positives = 33/54 (61%)
Frame = -1 / -1
Query: 510 LETHLLMDIGMMTMTKGRQRDEKEWSELFTKAGFSEYKILKEFGARVAFEVYP* 349
+ET LL D+ MM G +R+E+EW++LF AGF YKI R E+YP*
Sbjct: 342 IETQLLFDLEMMVAASGMERNEEEWAKLFFDAGFLNYKIHPVLDTRALIELYP* 181
Score = 51.5 bits (106), Expect(3) = 6e-021
Identities = 19/46 (41%), Positives = 29/46 (63%)
Frame = -1 / -1
Query: 705 INYVEGDMFSFIPPAQTXXXXXXXXXLTDEECVKLLAQCRKAIPSR 568
+ YV GDMF +PPA+ +DE+CVK+L +C++AI S+
Sbjct: 552 LKYVAGDMFQGVPPAEAILLKWMLHDWSDEDCVKILKRCKQAITSK 415
Score = 26.7 bits (52), Expect(3) = 6e-021
Identities = 8/17 (47%), Positives = 12/17 (70%)
Frame = -1 / -1
Query: 780 AFPHIKCTVLAPPKIIA 730
AFPH+ CTV P +++
Sbjct: 624 AFPHMDCTVFELPHVVS 574
>gb|AJ772970.1|AJ772970 AJ772970 Populus euphratica leaf 3-6 months Populus euphratica cDNA
clone P0000200003B11F1, mRNA sequence
Length = 597
Score = 78.0 bits (164), Expect(2) = 9e-021
Identities = 32/82 (39%), Positives = 44/82 (53%)
Frame = -1 / +2
Query: 813 GDDKGARAIVKAFPHIKCTVLAPPKIIATKPADGAMINYVEGDMFSFIPPAQTXXXXXXX 634
G A+ I +AFPH+ CTV P+++ A + YV GDMF +PPA
Sbjct: 176 GSGTMAKGIAEAFPHMDCTVFDLPRVVFDLQAGSKNLKYVGGDMFEAVPPADVILLKWTL 355
Query: 633 XXLTDEECVKLLAQCRKAIPSR 568
DE+CVK+L QC+ AI S+
Sbjct: 356 HDWNDEDCVKILKQCKHAIMSK 421
Score = 43.2 bits (88), Expect(2) = 9e-021
Identities = 17/33 (51%), Positives = 22/33 (66%)
Frame = -1 / +2
Query: 507 ETHLLMDIGMMTMTKGRQRDEKEWSELFTKAGF 409
ET LL D+ MM G +R+E EW++LF AGF
Sbjct: 494 ETQLLWDLEMMVTVTGLERNEMEWAKLFFDAGF 592
Database: Populus_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:49 PM
Number of letters in database: 203,408,664
Number of sequences in database: 369,679
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 737,889,219
Number of Sequences: 369679
Number of extensions: 11453405
Number of successful extensions: 75915
Number of sequences better than 1.0e-020: 10
length of database: 67,802,888
effective HSP length: 54
effective length of database: 47,840,222
effective search space used: 21623780344
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)