TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QCS9f07.yg.2.1
(394 letters)
Database: Pinus_nucl_with_EST.fasta
355,925 sequences; 217,277,237 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CO176149.1|CO176149 NDL1_60_C04.b1_A029 Needles control ... 34 0.49
gb|CX651539.1|CX651539 COLD1_53_C06.b1_A029 Root cold Pinus... 32 2.4
gb|CX651607.1|CX651607 COLD1_53_C06.g1_A029 Root cold Pinus... 32 2.4
gb|DR019766.1|DR019766 STRS1_32_F10.b1_A034 Shoot tip pitch... 32 2.4
gb|DR019842.1|DR019842 STRS1_32_F10.g1_A034 Shoot tip pitch... 32 2.4
gb|DR689501.1|DR689501 EST1079587 Normalized pine embryo li... 32 2.4
gb|DT635596.1|DT635596 EST1150527 Normalized pine embryo li... 32 2.4
gb|AW011513.1|AW011513 ST21G01 Pine TriplEx shoot tip libra... 31 3.3
gb|BQ107501.1|BQ107501 NXLV098_C07_F NXLV (Nsf Xylem Late w... 31 3.3
gb|DR012693.1|DR012693 HEAT1_14_A04.g1_A029 Root at 37 C fo... 31 3.3
gb|DR017197.1|DR017197 STRS1_14_E03.g1_A034 Shoot tip pitch... 31 3.3
gb|DR101987.1|DR101987 STRR1_77_F07.b1_A033 Stem Response R... 31 3.3
gb|DR690656.1|DR690656 EST1080742 Normalized pine embryo li... 31 3.3
gb|DT636351.1|DT636351 EST1151282 Normalized pine embryo li... 31 3.3
gb|DN462786.1|DN462786 EST958585 Sequencing ESTs from loblo... 30 6.2
gb|DR098477.1|DR098477 STRR1_45_F07.g1_A033 Stem Response R... 24 7.0
gb|BE662628.1|BE662628 ST88/ST88G11 Pine TriplEx shoot tip ... 28 8.0
gb|AW011280.1|AW011280 ST19A03 Pine TriplEx shoot tip libra... 30 8.5
gb|BM492483.1|BM492483 NXRV_025_H09_F NXRV (Nsf Xylem Root ... 30 8.5
gb|DN462735.1|DN462735 EST958534 Sequencing ESTs from loblo... 30 8.5
gb|DR051269.1|DR051269 RTBOR1_28_G05.g1_A029 Roots plus add... 30 8.5
gb|DT627169.1|DT627169 EST1160245 Sequencing ESTs from lobl... 30 8.5
>gb|CO176149.1|CO176149 NDL1_60_C04.b1_A029 Needles control Pinus taeda cDNA clone
NDL1_60_C04_A029 3', mRNA sequence
Length = 644
Score = 34.1 bits (68), Expect = 0.49
Identities = 14/46 (30%), Positives = 22/46 (47%)
Frame = -1 / -1
Query: 304 KIHLKEERTPKFTNRPCSCSSKKGTVYHLFIRERGRFKSESIYMRS 167
K +L + T F+N C+C+S G H ++ S+Y RS
Sbjct: 458 KSYL*KNHTDNFSNTQCTCNSWDGFTLHWLTELAAIWRENSVYFRS 321
>gb|CX651539.1|CX651539 COLD1_53_C06.b1_A029 Root cold Pinus taeda cDNA clone
COLD1_53_C06_A029 3', mRNA sequence
Length = 715
Score = 31.8 bits (63), Expect = 2.4
Identities = 10/24 (41%), Positives = 14/24 (58%)
Frame = -1 / -2
Query: 391 YDGPSDSFKALIDMAAVHSSCRLC 320
YDGP +L ++ VH SC+ C
Sbjct: 219 YDGPKSRTSSLFNLLHVHQSCKCC 148
>gb|CX651607.1|CX651607 COLD1_53_C06.g1_A029 Root cold Pinus taeda cDNA clone
COLD1_53_C06_A029 5', mRNA sequence
Length = 713
Score = 31.8 bits (63), Expect = 2.4
Identities = 10/24 (41%), Positives = 14/24 (58%)
Frame = -1 / -1
Query: 391 YDGPSDSFKALIDMAAVHSSCRLC 320
YDGP +L ++ VH SC+ C
Sbjct: 443 YDGPKSRTSSLFNLLHVHQSCKCC 372
>gb|DR019766.1|DR019766 STRS1_32_F10.b1_A034 Shoot tip pitch canker susceptible Pinus taeda
cDNA clone STRS1_32_F10_A034 3', mRNA sequence
Length = 788
Score = 31.8 bits (63), Expect = 2.4
Identities = 10/24 (41%), Positives = 14/24 (58%)
Frame = -1 / +2
Query: 391 YDGPSDSFKALIDMAAVHSSCRLC 320
YDGP +L ++ VH SC+ C
Sbjct: 347 YDGPKSRTSSLFNLLHVHQSCKCC 418
>gb|DR019842.1|DR019842 STRS1_32_F10.g1_A034 Shoot tip pitch canker susceptible Pinus taeda
cDNA clone STRS1_32_F10_A034 5', mRNA sequence
Length = 733
Score = 31.8 bits (63), Expect = 2.4
Identities = 10/24 (41%), Positives = 14/24 (58%)
Frame = -1 / +3
Query: 391 YDGPSDSFKALIDMAAVHSSCRLC 320
YDGP +L ++ VH SC+ C
Sbjct: 438 YDGPKSRTSSLFNLLHVHQSCKCC 509
>gb|DR689501.1|DR689501 EST1079587 Normalized pine embryo library, Lib_D Pinus taeda cDNA
clone PWACJ52 3' end, mRNA sequence
Length = 751
Score = 31.8 bits (63), Expect = 2.4
Identities = 10/24 (41%), Positives = 14/24 (58%)
Frame = -1 / +2
Query: 391 YDGPSDSFKALIDMAAVHSSCRLC 320
YDGP +L ++ VH SC+ C
Sbjct: 329 YDGPKSRTSSLFNLLHVHQSCKCC 400
>gb|DT635596.1|DT635596 EST1150527 Normalized pine embryo library, Lib_D Pinus taeda cDNA
clone PIMGI54 3' end, mRNA sequence
Length = 777
Score = 31.8 bits (63), Expect = 2.4
Identities = 10/24 (41%), Positives = 14/24 (58%)
Frame = -1 / +1
Query: 391 YDGPSDSFKALIDMAAVHSSCRLC 320
YDGP +L ++ VH SC+ C
Sbjct: 337 YDGPKSRTSSLFNLLHVHQSCKCC 408
>gb|AW011513.1|AW011513 ST21G01 Pine TriplEx shoot tip library Pinus taeda cDNA clone
ST21G01, mRNA sequence
Length = 492
Score = 31.3 bits (62), Expect = 3.3
Identities = 11/17 (64%), Positives = 15/17 (88%)
Frame = -2 / -1
Query: 285 KGLQNLQIDLVAVPAKR 235
KGLQ+L IDL+++P KR
Sbjct: 162 KGLQDLSIDLISIPRKR 112
>gb|BQ107501.1|BQ107501 NXLV098_C07_F NXLV (Nsf Xylem Late wood Vertical) Pinus taeda cDNA
clone NXLV098_C07 5' similar to Arabidopsis thaliana
sequence At1g72630 unknown protein see
http://mips.gsf.de/proj/thal/db/index.html, mRNA
sequence
Length = 655
Score = 31.3 bits (62), Expect = 3.3
Identities = 11/17 (64%), Positives = 15/17 (88%)
Frame = -2 / -1
Query: 285 KGLQNLQIDLVAVPAKR 235
KGLQ+L IDL+++P KR
Sbjct: 265 KGLQDLSIDLISIPRKR 215
>gb|DR012693.1|DR012693 HEAT1_14_A04.g1_A029 Root at 37 C for 24 hr Pinus taeda cDNA clone
HEAT1_14_A04_A029 5', mRNA sequence
Length = 722
Score = 31.3 bits (62), Expect = 3.3
Identities = 11/17 (64%), Positives = 15/17 (88%)
Frame = -2 / -3
Query: 285 KGLQNLQIDLVAVPAKR 235
KGLQ+L IDL+++P KR
Sbjct: 138 KGLQDLSIDLISIPRKR 88
>gb|DR017197.1|DR017197 STRS1_14_E03.g1_A034 Shoot tip pitch canker susceptible Pinus taeda
cDNA clone STRS1_14_E03_A034 5', mRNA sequence
Length = 683
Score = 31.3 bits (62), Expect = 3.3
Identities = 16/36 (44%), Positives = 19/36 (52%)
Frame = +2 / +3
Query: 134 CRFQGSQC*LARSHINAL*LKPPSLSDKQVVNCPLF 241
C G * RSHIN L L P D Q+++ PLF
Sbjct: 540 CTSDGRFD*ECRSHINCLRLSPIGWHDIQLISPPLF 647
>gb|DR101987.1|DR101987 STRR1_77_F07.b1_A033 Stem Response Resistant Pinus taeda cDNA clone
STRR1_77_F07_A033 3', mRNA sequence
Length = 792
Score = 31.3 bits (62), Expect = 3.3
Identities = 16/36 (44%), Positives = 19/36 (52%)
Frame = +2 / +3
Query: 134 CRFQGSQC*LARSHINAL*LKPPSLSDKQVVNCPLF 241
C G * RSHIN L L P D Q+++ PLF
Sbjct: 645 CTSDGRFD*ECRSHINCLRLSPIGWHDIQLISPPLF 752
>gb|DR690656.1|DR690656 EST1080742 Normalized pine embryo library, Lib_D Pinus taeda cDNA
clone PWACX69 3' end, mRNA sequence
Length = 859
Score = 31.3 bits (62), Expect = 3.3
Identities = 11/17 (64%), Positives = 15/17 (88%)
Frame = -2 / -2
Query: 285 KGLQNLQIDLVAVPAKR 235
KGLQ+L IDL+++P KR
Sbjct: 387 KGLQDLSIDLISIPRKR 337
>gb|DT636351.1|DT636351 EST1151282 Normalized pine embryo library, Lib_D Pinus taeda cDNA
clone PIMGQ79 3' end, mRNA sequence
Length = 816
Score = 31.3 bits (62), Expect = 3.3
Identities = 11/17 (64%), Positives = 15/17 (88%)
Frame = -2 / -2
Query: 285 KGLQNLQIDLVAVPAKR 235
KGLQ+L IDL+++P KR
Sbjct: 395 KGLQDLSIDLISIPRKR 345
>gb|DN462786.1|DN462786 EST958585 Sequencing ESTs from loblolly pine embryos Pinus taeda
cDNA clone RPIIQ70 5' end, mRNA sequence
Length = 888
Score = 30.4 bits (60), Expect = 6.2
Identities = 12/34 (35%), Positives = 17/34 (50%)
Frame = +2 / -2
Query: 56 IPTSNTRWPFILPYRNVVEGPKFTEHCRFQGSQC 157
+P S T W F LP V + P + C F+ +C
Sbjct: 275 LPPSLT*WGFALPLTTVNKTPNYRGRCAFRPWRC 174
>gb|DR098477.1|DR098477 STRR1_45_F07.g1_A033 Stem Response Resistant Pinus taeda cDNA clone
STRR1_45_F07_A033 5', mRNA sequence
Length = 497
Score = 24.4 bits (47), Expect(2) = 7.0
Identities = 9/12 (75%), Positives = 11/12 (91%)
Frame = -3 / +3
Query: 305 QDSFKGGKDSKI 270
QD+ KGGKDSK+
Sbjct: 195 QDNRKGGKDSKM 230
Score = 22.1 bits (42), Expect(2) = 7.0
Identities = 7/13 (53%), Positives = 10/13 (76%)
Frame = -2 / +2
Query: 357 STWQRFIHLVVFV 319
S WQ+F+ + VFV
Sbjct: 89 SKWQKFLKIPVFV 127
>gb|BE662628.1|BE662628 ST88/ST88G11 Pine TriplEx shoot tip library Pinus taeda cDNA clone
ST88/ST88G11, mRNA sequence
Length = 312
Score = 27.6 bits (54), Expect(2) = 8.0
Identities = 11/27 (40%), Positives = 18/27 (66%)
Frame = -3 / -2
Query: 380 I*FFQSSYRHGSGSFILSSLYTYCYQD 300
I* + + Y H SG+ LS+L +C++D
Sbjct: 224 I*RWGTGYTHSSGNCTLSALQGWCWRD 144
Score = 18.0 bits (33), Expect(2) = 8.0
Identities = 6/13 (46%), Positives = 8/13 (61%)
Frame = -1 / -3
Query: 259 PCSCSSKKGTVYH 221
P +CSS+ YH
Sbjct: 46 PTTCSSRFCLYYH 8
>gb|AW011280.1|AW011280 ST19A03 Pine TriplEx shoot tip library Pinus taeda cDNA clone
ST19A03, mRNA sequence
Length = 601
Score = 29.9 bits (59), Expect = 8.5
Identities = 11/17 (64%), Positives = 14/17 (82%)
Frame = -2 / -3
Query: 285 KGLQNLQIDLVAVPAKR 235
KGLQ+L IDL ++P KR
Sbjct: 344 KGLQDLSIDLTSIPRKR 294
>gb|BM492483.1|BM492483 NXRV_025_H09_F NXRV (Nsf Xylem Root wood Vertical) Pinus taeda cDNA
clone NXRV_025_H09 5', mRNA sequence
Length = 634
Score = 29.9 bits (59), Expect = 8.5
Identities = 11/15 (73%), Positives = 12/15 (80%)
Frame = -3 / -1
Query: 359 YRHGSGSFILSSLYT 315
YRHGSGS +L LYT
Sbjct: 289 YRHGSGSLVLK*LYT 245
>gb|DN462735.1|DN462735 EST958534 Sequencing ESTs from loblolly pine embryos Pinus taeda
cDNA clone RPIIQ08 5' end, mRNA sequence
Length = 914
Score = 29.9 bits (59), Expect = 8.5
Identities = 10/28 (35%), Positives = 17/28 (60%)
Frame = -1 / +3
Query: 292 KEERTPKFTNRPCSCSSKKGTVYHLFIR 209
+EE+ K T+R C +++ G +H F R
Sbjct: 549 REEKCEKDTSRTCEAAARSGQSHHYFTR 632
>gb|DR051269.1|DR051269 RTBOR1_28_G05.g1_A029 Roots plus added boron Pinus taeda cDNA clone
RTBOR1_28_G05_A029 5', mRNA sequence
Length = 669
Score = 29.9 bits (59), Expect = 8.5
Identities = 12/25 (48%), Positives = 17/25 (68%)
Frame = -2 / +3
Query: 288 RKGLQNLQIDLVAVPAKRGQFTTCL 214
R+G Q LQ DL + P ++ Q +TCL
Sbjct: 531 RRGQQLLQFDLASPPEEQDQGSTCL 605
>gb|DT627169.1|DT627169 EST1160245 Sequencing ESTs from loblolly pine embryos Pinus taeda
cDNA clone PIMAV68 5' end, mRNA sequence
Length = 985
Score = 29.9 bits (59), Expect = 8.5
Identities = 10/28 (35%), Positives = 17/28 (60%)
Frame = -1 / +3
Query: 292 KEERTPKFTNRPCSCSSKKGTVYHLFIR 209
+EE+ K T+R C +++ G +H F R
Sbjct: 549 REEKCEKDTSRTCEAAARSGQSHHYFTR 632
Database: Pinus_nucl_with_EST.fasta
Posted date: May 2, 2006 3:25 PM
Number of letters in database: 217,277,237
Number of sequences in database: 355,925
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 213,106,991
Number of Sequences: 355925
Number of extensions: 2736381
Number of successful extensions: 62249
Number of sequences better than 10.0: 28
length of database: 72,425,745
effective HSP length: 50
effective length of database: 54,629,495
effective search space used: 4370359600
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)