TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QCA17a05.yg.2.1
(548 letters)
Database: Pinus_nucl_with_EST.fasta
355,925 sequences; 217,277,237 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CX712891.1|CX712891 RTPQ1_5_B06.g1_A032 Roots treated wi... 172 1e-042
gb|DR111377.1|DR111377 RTS1_17_F12.b1_A029 Roots minus sulf... 172 1e-042
gb|DR116979.1|DR116979 RTMG1_4_B01.b1_A029 Roots minus magn... 172 1e-042
gb|DR117061.1|DR117061 RTMG1_4_B01.g1_A029 Roots minus magn... 172 1e-042
gb|DR694121.1|DR694121 EST1084211 Normalized pine embryo li... 163 7e-040
gb|DT638404.1|DT638404 EST1153335 Normalized pine embryo li... 163 7e-040
>gb|CX712891.1|CX712891 RTPQ1_5_B06.g1_A032 Roots treated with paraquat Pinus taeda cDNA
clone RTPQ1_5_B06_A032 5', mRNA sequence
Length = 781
Score = 172 bits (371), Expect = 1e-042
Identities = 61/89 (68%), Positives = 75/89 (84%)
Frame = -3 / -1
Query: 546 VISAGIARDVASRHTNHSLRLFKMEDVSMGMWVEDYNASAPVQYVHSWRFCQFGCVDNYF 367
+I+ IA + S+H N +LRLFKMEDVSMGMWVE +NAS PVQY H W+FCQFGC+D+Y+
Sbjct: 418 IITRDIANFIVSQHKNQTLRLFKMEDVSMGMWVEQFNASTPVQYSHDWKFCQFGCIDDYY 239
Query: 366 TAHYQSPRQMLCLWEKLSAGRAHCCNYR* 280
TAHYQSPRQM+C+W+KLS G+A CCN R*
Sbjct: 238 TAHYQSPRQMICMWDKLSKGKAQCCNVR* 152
Score = 113 bits (241), Expect = 1e-024
Identities = 51/85 (60%), Positives = 56/85 (65%)
Frame = +2 / +3
Query: 278 CYL*LQQCALPADSFSQRHSICLGDW*CAVK*LSTQPNWQNLQLWTYCTGALAL*SSTHM 457
C+L LQ A P +S S H ICLGDW*CAV * S PNWQN Q W Y TG AL STH+
Sbjct: 150 CHLTLQH*AFPLESLSHIHIICLGDW*CAV**SSMHPNWQNFQSWEY*TGVDALNCSTHI 329
Query: 458 PMLTSSILNSRREWFVCRDATSLAM 532
PMLTSSILN+ R F+C D AM
Sbjct: 330 PMLTSSILNNLRV*FLC*DTIKFAM 404
>gb|DR111377.1|DR111377 RTS1_17_F12.b1_A029 Roots minus sulfur Pinus taeda cDNA clone
RTS1_17_F12_A029 3', mRNA sequence
Length = 674
Score = 172 bits (371), Expect = 1e-042
Identities = 61/89 (68%), Positives = 75/89 (84%)
Frame = -3 / +2
Query: 546 VISAGIARDVASRHTNHSLRLFKMEDVSMGMWVEDYNASAPVQYVHSWRFCQFGCVDNYF 367
+I+ IA + S+H N +LRLFKMEDVSMGMWVE +NAS PVQY H W+FCQFGC+D+Y+
Sbjct: 77 IITRDIANFIVSQHKNQTLRLFKMEDVSMGMWVEQFNASTPVQYSHDWKFCQFGCIDDYY 256
Query: 366 TAHYQSPRQMLCLWEKLSAGRAHCCNYR* 280
TAHYQSPRQM+C+W+KLS G+A CCN R*
Sbjct: 257 TAHYQSPRQMICMWDKLSKGKAQCCNVR* 343
Score = 113 bits (241), Expect = 1e-024
Identities = 51/85 (60%), Positives = 56/85 (65%)
Frame = +2 / -3
Query: 278 CYL*LQQCALPADSFSQRHSICLGDW*CAVK*LSTQPNWQNLQLWTYCTGALAL*SSTHM 457
C+L LQ A P +S S H ICLGDW*CAV * S PNWQN Q W Y TG AL STH+
Sbjct: 345 CHLTLQH*AFPLESLSHIHIICLGDW*CAV**SSMHPNWQNFQSWEY*TGVDALNCSTHI 166
Query: 458 PMLTSSILNSRREWFVCRDATSLAM 532
PMLTSSILN+ R F+C D AM
Sbjct: 165 PMLTSSILNNLRV*FLC*DTIKFAM 91
>gb|DR116979.1|DR116979 RTMG1_4_B01.b1_A029 Roots minus magnesium Pinus taeda cDNA clone
RTMG1_4_B01_A029 3', mRNA sequence
Length = 725
Score = 172 bits (371), Expect = 1e-042
Identities = 61/89 (68%), Positives = 75/89 (84%)
Frame = -3 / +3
Query: 546 VISAGIARDVASRHTNHSLRLFKMEDVSMGMWVEDYNASAPVQYVHSWRFCQFGCVDNYF 367
+I+ IA + S+H N +LRLFKMEDVSMGMWVE +NAS PVQY H W+FCQFGC+D+Y+
Sbjct: 159 IITRDIANFIVSQHKNQTLRLFKMEDVSMGMWVEQFNASTPVQYSHDWKFCQFGCIDDYY 338
Query: 366 TAHYQSPRQMLCLWEKLSAGRAHCCNYR* 280
TAHYQSPRQM+C+W+KLS G+A CCN R*
Sbjct: 339 TAHYQSPRQMICMWDKLSKGKAQCCNVR* 425
Score = 113 bits (241), Expect = 1e-024
Identities = 51/85 (60%), Positives = 56/85 (65%)
Frame = +2 / -2
Query: 278 CYL*LQQCALPADSFSQRHSICLGDW*CAVK*LSTQPNWQNLQLWTYCTGALAL*SSTHM 457
C+L LQ A P +S S H ICLGDW*CAV * S PNWQN Q W Y TG AL STH+
Sbjct: 427 CHLTLQH*AFPLESLSHIHIICLGDW*CAV**SSMHPNWQNFQSWEY*TGVDALNCSTHI 248
Query: 458 PMLTSSILNSRREWFVCRDATSLAM 532
PMLTSSILN+ R F+C D AM
Sbjct: 247 PMLTSSILNNLRV*FLC*DTIKFAM 173
>gb|DR117061.1|DR117061 RTMG1_4_B01.g1_A029 Roots minus magnesium Pinus taeda cDNA clone
RTMG1_4_B01_A029 5', mRNA sequence
Length = 800
Score = 172 bits (371), Expect = 1e-042
Identities = 61/89 (68%), Positives = 75/89 (84%)
Frame = -3 / +3
Query: 546 VISAGIARDVASRHTNHSLRLFKMEDVSMGMWVEDYNASAPVQYVHSWRFCQFGCVDNYF 367
+I+ IA + S+H N +LRLFKMEDVSMGMWVE +NAS PVQY H W+FCQFGC+D+Y+
Sbjct: 288 IITRDIANFIVSQHKNQTLRLFKMEDVSMGMWVEQFNASTPVQYSHDWKFCQFGCIDDYY 467
Query: 366 TAHYQSPRQMLCLWEKLSAGRAHCCNYR* 280
TAHYQSPRQM+C+W+KLS G+A CCN R*
Sbjct: 468 TAHYQSPRQMICMWDKLSKGKAQCCNVR* 554
Score = 113 bits (241), Expect = 1e-024
Identities = 51/85 (60%), Positives = 56/85 (65%)
Frame = +2 / -2
Query: 278 CYL*LQQCALPADSFSQRHSICLGDW*CAVK*LSTQPNWQNLQLWTYCTGALAL*SSTHM 457
C+L LQ A P +S S H ICLGDW*CAV * S PNWQN Q W Y TG AL STH+
Sbjct: 556 CHLTLQH*AFPLESLSHIHIICLGDW*CAV**SSMHPNWQNFQSWEY*TGVDALNCSTHI 377
Query: 458 PMLTSSILNSRREWFVCRDATSLAM 532
PMLTSSILN+ R F+C D AM
Sbjct: 376 PMLTSSILNNLRV*FLC*DTIKFAM 302
>gb|DR694121.1|DR694121 EST1084211 Normalized pine embryo library, Lib_D Pinus taeda cDNA
clone PWAE837 3' end, mRNA sequence
Length = 746
Score = 163 bits (351), Expect = 7e-040
Identities = 57/86 (66%), Positives = 72/86 (83%)
Frame = -3 / +3
Query: 546 VISAGIARDVASRHTNHSLRLFKMEDVSMGMWVEDYNASAPVQYVHSWRFCQFGCVDNYF 367
+IS +A V S+H NH+LRLFKMEDVSMGMWVE +NAS+ VQY H+W+FCQ+GC D+Y+
Sbjct: 123 IISNDVANFVVSQHANHTLRLFKMEDVSMGMWVEQFNASSRVQYSHNWKFCQYGCTDDYY 302
Query: 366 TAHYQSPRQMLCLWEKLSAGRAHCCN 289
TAHYQSPRQMLC+W+KL G+ CC+
Sbjct: 303 TAHYQSPRQMLCMWDKLLKGKVECCS 380
>gb|DT638404.1|DT638404 EST1153335 Normalized pine embryo library, Lib_D Pinus taeda cDNA
clone PIMHE72 3' end, mRNA sequence
Length = 824
Score = 163 bits (351), Expect = 7e-040
Identities = 57/86 (66%), Positives = 72/86 (83%)
Frame = -3 / +2
Query: 546 VISAGIARDVASRHTNHSLRLFKMEDVSMGMWVEDYNASAPVQYVHSWRFCQFGCVDNYF 367
+IS +A V S+H NH+LRLFKMEDVSMGMWVE +NAS+ VQY H+W+FCQ+GC D+Y+
Sbjct: 200 IISNDVANFVVSQHANHTLRLFKMEDVSMGMWVEQFNASSRVQYSHNWKFCQYGCTDDYY 379
Query: 366 TAHYQSPRQMLCLWEKLSAGRAHCCN 289
TAHYQSPRQMLC+W+KL G+ CC+
Sbjct: 380 TAHYQSPRQMLCMWDKLLKGKVECCS 457
Database: Pinus_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:45 PM
Number of letters in database: 217,277,237
Number of sequences in database: 355,925
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 330,641,199
Number of Sequences: 355925
Number of extensions: 5310638
Number of successful extensions: 55559
Number of sequences better than 1.0e-020: 6
length of database: 72,425,745
effective HSP length: 51
effective length of database: 54,273,570
effective search space used: 7109837670
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)