TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QBTB.068P22F020924.3.1
(714 letters)
Database: Pinus_nucl_with_EST.fasta
355,925 sequences; 217,277,237 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CO165974.1|CO165974 FLD1_58_A08.g1_A029 Root flooded Pin... 133 8e-052
gb|DT635127.1|DT635127 EST1150058 Normalized pine embryo li... 133 7e-044
gb|DR109740.1|DR109740 RTS1_4_G12.b1_A029 Roots minus sulfu... 87 3e-034
gb|CF471390.1|CF471390 RTDS1_3_B03.b1_A015 Drought-stressed... 87 3e-034
gb|CO167344.1|CO167344 FLD1_68_H07.b1_A029 Root flooded Pin... 133 9e-031
gb|DR689123.1|DR689123 EST1079209 Normalized pine embryo li... 133 9e-031
gb|CV031406.1|CV031406 RTNACL1_1_H09.b1_A029 Roots plus add... 70 2e-030
gb|CR394310.1|CR394310 CR394310 RN Pinus pinaster cDNA clon... 79 8e-030
gb|CF390618.1|CF390618 RTDR2_20_D07.g1_A021 Loblolly pine r... 100 5e-029
gb|CF473146.1|CF473146 RTWW2_1_H06.b1_A021 Well-watered lob... 100 5e-026
gb|DR011124.1|DR011124 HEAT1_3_D03.g1_A029 Root at 37 C for... 109 3e-023
gb|CR394251.1|CR394251 CR394251 RN Pinus pinaster cDNA clon... 79 1e-022
gb|CO199527.1|CO199527 GEO2_1_G08.g1_A032 Root gravitropism... 105 3e-022
gb|DT635415.1|DT635415 EST1150346 Normalized pine embryo li... 72 8e-021
>gb|CO165974.1|CO165974 FLD1_58_A08.g1_A029 Root flooded Pinus taeda cDNA clone
FLD1_58_A08_A029 5', mRNA sequence
Length = 830
Score = 133 bits (285), Expect(2) = 8e-052
Identities = 52/75 (69%), Positives = 64/75 (85%)
Frame = +2 / +1
Query: 488 DQSHSMSGNGASAFDMEYARWLEEHNRQISELRAGVSAHASDTDLRSVVDKIMSHYDEIF 667
DQSHSMSGNGA AFDMEYARWL+EH+RQI+ELR+ V++H D +LR +V+ +M HYDEIF
Sbjct: 448 DQSHSMSGNGALAFDMEYARWLDEHHRQINELRSAVNSHVGDNELRVLVEGVMGHYDEIF 627
Query: 668 RLKGNAAKADVFHVL 712
RLK A KADVFH++
Sbjct: 628 RLKTVAXKADVFHLV 672
Score = 90.9 bits (192), Expect(2) = 8e-052
Identities = 42/58 (72%), Positives = 44/58 (75%)
Frame = +2 / +1
Query: 311 HGDQKTLRRLAQNREAARKSRLRKKAYVQQLENSRLKLTXXXXXXXXXXXXGIFISSS 484
+GDQKTLRRLAQNREAARKSRLRKKAYVQQLE+SRLKLT GIFI S
Sbjct: 262 NGDQKTLRRLAQNREAARKSRLRKKAYVQQLESSRLKLTQLEQELQRARQQGIFIGGS 435
>gb|DT635127.1|DT635127 EST1150058 Normalized pine embryo library, Lib_D Pinus taeda cDNA
clone PIMGD46 3' end, mRNA sequence
Length = 824
Score = 133 bits (286), Expect(2) = 7e-044
Identities = 52/75 (69%), Positives = 65/75 (86%)
Frame = +2 / +2
Query: 488 DQSHSMSGNGASAFDMEYARWLEEHNRQISELRAGVSAHASDTDLRSVVDKIMSHYDEIF 667
DQSHSMSGNGA AFDMEYARWL+EH+RQI+ELR+ V++H D +LR +V+ +M HYDEIF
Sbjct: 158 DQSHSMSGNGALAFDMEYARWLDEHHRQINELRSAVNSHVGDNELRVLVEGVMGHYDEIF 337
Query: 668 RLKGNAAKADVFHVL 712
RLK A+KADVFH++
Sbjct: 338 RLKTVASKADVFHLV 382
Score = 63.8 bits (133), Expect(2) = 7e-044
Identities = 30/47 (63%), Positives = 33/47 (70%)
Frame = +2 / +2
Query: 344 QNREAARKSRLRKKAYVQQLENSRLKLTXXXXXXXXXXXXGIFISSS 484
++REAARKSRLRKKAYVQQLE+SRLKLT GIFI S
Sbjct: 5 RDREAARKSRLRKKAYVQQLESSRLKLTQLEQELQRARQQGIFIGGS 145
>gb|DR109740.1|DR109740 RTS1_4_G12.b1_A029 Roots minus sulfur Pinus taeda cDNA clone
RTS1_4_G12_A029 3', mRNA sequence
Length = 755
Score = 86.8 bits (183), Expect(2) = 3e-034
Identities = 32/67 (47%), Positives = 47/67 (70%)
Frame = +2 / -3
Query: 512 NGASAFDMEYARWLEEHNRQISELRAGVSAHASDTDLRSVVDKIMSHYDEIFRLKGNAAK 691
+ A+AFDMEY RWLEEH+R + +LRA + H D DLR V+ ++HYD++ +K AK
Sbjct: 459 SSAAAFDMEYQRWLEEHHRHMCDLRAALQEHLPDNDLRVFVENCLAHYDDLLHIKIVGAK 280
Query: 692 ADVFHVL 712
DVF+++
Sbjct: 279 TDVFYLV 259
Score = 78.5 bits (165), Expect(2) = 3e-034
Identities = 35/53 (66%), Positives = 39/53 (73%)
Frame = +2 / -3
Query: 317 DQKTLRRLAQNREAARKSRLRKKAYVQQLENSRLKLTXXXXXXXXXXXXGIFI 475
D KTLRRLAQNREAARKSRLRKKAYVQQLE+SR+KL G+F+
Sbjct: 684 DAKTLRRLAQNREAARKSRLRKKAYVQQLESSRIKLAQLEQELQRARTQGVFM 526
>gb|CF471390.1|CF471390 RTDS1_3_B03.b1_A015 Drought-stressed loblolly pine roots DS1 Pinus
taeda cDNA clone RTDS1_3_B03_A015 3', mRNA sequence
Length = 663
Score = 86.8 bits (183), Expect(2) = 3e-034
Identities = 32/67 (47%), Positives = 47/67 (70%)
Frame = +2 / -3
Query: 512 NGASAFDMEYARWLEEHNRQISELRAGVSAHASDTDLRSVVDKIMSHYDEIFRLKGNAAK 691
+ A+AFDMEY RWLEEH+R + +LRA + H D DLR V+ ++HYD++ +K AK
Sbjct: 202 SSAAAFDMEYQRWLEEHHRHMCDLRAALQEHLPDNDLRVFVENCLAHYDDLLHIKIVGAK 23
Query: 692 ADVFHVL 712
DVF+++
Sbjct: 22 TDVFYLV 2
Score = 78.5 bits (165), Expect(2) = 3e-034
Identities = 35/53 (66%), Positives = 39/53 (73%)
Frame = +2 / -3
Query: 317 DQKTLRRLAQNREAARKSRLRKKAYVQQLENSRLKLTXXXXXXXXXXXXGIFI 475
D KTLRRLAQNREAARKSRLRKKAYVQQLE+SR+KL G+F+
Sbjct: 427 DAKTLRRLAQNREAARKSRLRKKAYVQQLESSRIKLAQLEQELQRARTQGVFM 269
>gb|CO167344.1|CO167344 FLD1_68_H07.b1_A029 Root flooded Pinus taeda cDNA clone
FLD1_68_H07_A029 3', mRNA sequence
Length = 867
Score = 133 bits (286), Expect = 9e-031
Identities = 52/75 (69%), Positives = 65/75 (86%)
Frame = +2 / -1
Query: 488 DQSHSMSGNGASAFDMEYARWLEEHNRQISELRAGVSAHASDTDLRSVVDKIMSHYDEIF 667
DQSHSMSGNGA AFDMEYARWL+EH+RQI+ELR+ V++H D +LR +V+ +M HYDEIF
Sbjct: 786 DQSHSMSGNGALAFDMEYARWLDEHHRQINELRSAVNSHVGDNELRVLVEGVMGHYDEIF 607
Query: 668 RLKGNAAKADVFHVL 712
RLK A+KADVFH++
Sbjct: 606 RLKTVASKADVFHLV 562
>gb|DR689123.1|DR689123 EST1079209 Normalized pine embryo library, Lib_D Pinus taeda cDNA
clone PWACE46 3' end, mRNA sequence
Length = 708
Score = 133 bits (286), Expect = 9e-031
Identities = 52/75 (69%), Positives = 65/75 (86%)
Frame = +2 / +1
Query: 488 DQSHSMSGNGASAFDMEYARWLEEHNRQISELRAGVSAHASDTDLRSVVDKIMSHYDEIF 667
DQSHSMSGNGA AFDMEYARWL+EH+RQI+ELR+ V++H D +LR +V+ +M HYDEIF
Sbjct: 10 DQSHSMSGNGALAFDMEYARWLDEHHRQINELRSAVNSHVGDNELRVLVEGVMGHYDEIF 189
Query: 668 RLKGNAAKADVFHVL 712
RLK A+KADVFH++
Sbjct: 190 RLKTVASKADVFHLV 234
>gb|CV031406.1|CV031406 RTNACL1_1_H09.b1_A029 Roots plus added NaCl Pinus taeda cDNA clone
RTNACL1_1_H09_A029 3', mRNA sequence
Length = 687
Score = 70.3 bits (147), Expect(3) = 2e-030
Identities = 28/54 (51%), Positives = 41/54 (75%)
Frame = +2 / +2
Query: 551 LEEHNRQISELRAGVSAHASDTDLRSVVDKIMSHYDEIFRLKGNAAKADVFHVL 712
L+ QIS+LRA ++AH +D +LR +V+ M+HYD +FR+K AAKADVFH++
Sbjct: 236 LKNKTXQISDLRAALTAHVTDIELRILVEGGMAHYDALFRMKSVAAKADVFHLV 397
Score = 60.2 bits (125), Expect(3) = 2e-030
Identities = 26/26 (100%), Positives = 26/26 (100%)
Frame = +2 / +3
Query: 332 RRLAQNREAARKSRLRKKAYVQQLEN 409
RRLAQNREAARKSRLRKKAYVQQLEN
Sbjct: 3 RRLAQNREAARKSRLRKKAYVQQLEN 80
Score = 40.0 bits (81), Expect(3) = 2e-030
Identities = 13/28 (46%), Positives = 20/28 (71%)
Frame = +2 / +1
Query: 485 VDQSHSMSGNGASAFDMEYARWLEEHNR 568
+ ++ + GA+AFD+EYA W+EE NR
Sbjct: 169 ISSANGAANAGAAAFDLEYAHWVEEQNR 252
>gb|CR394310.1|CR394310 CR394310 RN Pinus pinaster cDNA clone RN76F04, mRNA sequence
Length = 630
Score = 78.5 bits (165), Expect(2) = 8e-030
Identities = 35/53 (66%), Positives = 39/53 (73%)
Frame = +2 / +1
Query: 317 DQKTLRRLAQNREAARKSRLRKKAYVQQLENSRLKLTXXXXXXXXXXXXGIFI 475
D KTLRRLAQNREAARKSRLRKKAYVQQLE+SR+KL G+F+
Sbjct: 247 DAKTLRRLAQNREAARKSRLRKKAYVQQLESSRIKLAQLEQELQRARTQGVFM 405
Score = 72.1 bits (151), Expect(2) = 8e-030
Identities = 26/51 (50%), Positives = 36/51 (70%)
Frame = +2 / +1
Query: 512 NGASAFDMEYARWLEEHNRQISELRAGVSAHASDTDLRSVVDKIMSHYDEI 664
+ A+AFDMEY RWLEEH R + +LRA + H D DLR V+ ++HYD++
Sbjct: 472 SSAAAFDMEYQRWLEEHQRHMCDLRAALQEHLPDNDLRVFVENWLAHYDDL 624
>gb|CF390618.1|CF390618 RTDR2_20_D07.g1_A021 Loblolly pine roots recovering from drought
DR2 Pinus taeda cDNA clone RTDR2_20_D07_A021 5', mRNA
sequence
Length = 797
Score = 100 bits (214), Expect(2) = 5e-029
Identities = 41/75 (54%), Positives = 53/75 (70%)
Frame = +2 / +1
Query: 488 DQSHSMSGNGASAFDMEYARWLEEHNRQISELRAGVSAHASDTDLRSVVDKIMSHYDEIF 667
DQ H GA+ FDMEY+RWLEE++ ++ ELRA + AH D DLR ++D + YDEIF
Sbjct: 136 DQGHPGLSPGAAMFDMEYSRWLEENHGRMCELRAALQAHIPDNDLRPLLDNCLRLYDEIF 315
Query: 668 RLKGNAAKADVFHVL 712
RLK AAK DVFH++
Sbjct: 316 RLKSIAAKTDVFHLV 360
Score = 46.9 bits (96), Expect(2) = 5e-029
Identities = 19/41 (46%), Positives = 28/41 (68%)
Frame = +2 / +1
Query: 359 ARKSRLRKKAYVQQLENSRLKLTXXXXXXXXXXXXGIFISS 481
+++SRLRKKAYVQQLE+SR+KL+ G+F+ +
Sbjct: 1 SKESRLRKKAYVQQLESSRMKLSQLEQDLQRARTQGVFLGA 123
>gb|CF473146.1|CF473146 RTWW2_1_H06.b1_A021 Well-watered loblolly pine roots WW2 Pinus
taeda cDNA clone RTWW2_1_H06_A021 3', mRNA sequence
Length = 630
Score = 100 bits (214), Expect(2) = 5e-026
Identities = 41/75 (54%), Positives = 53/75 (70%)
Frame = +2 / -1
Query: 488 DQSHSMSGNGASAFDMEYARWLEEHNRQISELRAGVSAHASDTDLRSVVDKIMSHYDEIF 667
DQ H GA+ FDMEY+RWLEE++ ++ ELRA + AH D DLR ++D + YDEIF
Sbjct: 513 DQGHPGLSPGAAMFDMEYSRWLEENHGRMCELRAALQAHIPDNDLRPLLDNCLRLYDEIF 334
Query: 668 RLKGNAAKADVFHVL 712
RLK AAK DVFH++
Sbjct: 333 RLKSIAAKTDVFHLV 289
Score = 36.8 bits (74), Expect(2) = 5e-026
Identities = 15/34 (44%), Positives = 21/34 (61%)
Frame = +2 / -1
Query: 380 KKAYVQQLENSRLKLTXXXXXXXXXXXXGIFISS 481
KKAYVQQLE+SR+KL+ G+F+ +
Sbjct: 627 KKAYVQQLESSRMKLSQLEQDLQRARTQGVFLGA 526
>gb|DR011124.1|DR011124 HEAT1_3_D03.g1_A029 Root at 37 C for 24 hr Pinus taeda cDNA clone
HEAT1_3_D03_A029 5', mRNA sequence
Length = 705
Score = 109 bits (232), Expect = 3e-023
Identities = 42/65 (64%), Positives = 55/65 (84%)
Frame = +2 / +1
Query: 518 ASAFDMEYARWLEEHNRQISELRAGVSAHASDTDLRSVVDKIMSHYDEIFRLKGNAAKAD 697
A AFDMEYARWL+EH+RQI+ELR+ V++H D +LR +V+ +M HYDEIFRLK A+KAD
Sbjct: 1 ALAFDMEYARWLDEHHRQINELRSAVNSHVGDNELRVLVEGVMGHYDEIFRLKTVASKAD 180
Query: 698 VFHVL 712
VFH++
Sbjct: 181 VFHLV 195
>gb|CR394251.1|CR394251 CR394251 RN Pinus pinaster cDNA clone RN75G01, mRNA sequence
Length = 548
Score = 78.5 bits (165), Expect(2) = 1e-022
Identities = 35/53 (66%), Positives = 39/53 (73%)
Frame = +2 / +3
Query: 317 DQKTLRRLAQNREAARKSRLRKKAYVQQLENSRLKLTXXXXXXXXXXXXGIFI 475
D KTLRRLAQNREAARKSRLRKKAYVQQLE+SR+KL G+F+
Sbjct: 240 DAKTLRRLAQNREAARKSRLRKKAYVQQLESSRIKLAQLEQELQRARTQGVFM 398
Score = 47.8 bits (98), Expect(2) = 1e-022
Identities = 17/28 (60%), Positives = 23/28 (82%)
Frame = +2 / +3
Query: 512 NGASAFDMEYARWLEEHNRQISELRAGV 595
+ A+AFDMEY RWLEEH+R + +LRA +
Sbjct: 465 SSAAAFDMEYQRWLEEHHRHMCDLRAAL 548
>gb|CO199527.1|CO199527 GEO2_1_G08.g1_A032 Root gravitropism October 2003 test Pinus taeda
cDNA clone GEO2_1_G08_A032 5', mRNA sequence
Length = 740
Score = 105 bits (224), Expect = 3e-022
Identities = 39/65 (60%), Positives = 53/65 (81%)
Frame = +2 / +2
Query: 518 ASAFDMEYARWLEEHNRQISELRAGVSAHASDTDLRSVVDKIMSHYDEIFRLKGNAAKAD 697
A+AFDMEY RW+EE NRQ ELRA + AH +D++LR +VD ++HYDE+FR+KG A+K D
Sbjct: 2 AAAFDMEYGRWVEEQNRQARELRASLQAHLTDSELRVLVDNAIAHYDELFRMKGAASKLD 181
Query: 698 VFHVL 712
VFH++
Sbjct: 182 VFHLM 196
>gb|DT635415.1|DT635415 EST1150346 Normalized pine embryo library, Lib_D Pinus taeda cDNA
clone PIMGG58 3' end, mRNA sequence
Length = 803
Score = 71.6 bits (150), Expect(2) = 8e-021
Identities = 31/36 (86%), Positives = 34/36 (94%)
Frame = +2 / +1
Query: 317 DQKTLRRLAQNREAARKSRLRKKAYVQQLENSRLKL 424
+ K LRRLAQNREAARKSRLRKKAYVQQLE+SR+KL
Sbjct: 466 NNKVLRRLAQNREAARKSRLRKKAYVQQLESSRIKL 573
Score = 48.7 bits (100), Expect(2) = 8e-021
Identities = 18/27 (66%), Positives = 22/27 (81%)
Frame = +2 / +1
Query: 506 SGNGASAFDMEYARWLEEHNRQISELR 586
+G+GA+AF MEY RW+EE NRQ ELR
Sbjct: 646 NGSGAAAFGMEYGRWVEEQNRQARELR 726
Database: Pinus_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:45 PM
Number of letters in database: 217,277,237
Number of sequences in database: 355,925
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 324,487,209
Number of Sequences: 355925
Number of extensions: 4146801
Number of successful extensions: 28149
Number of sequences better than 1.0e-020: 17
length of database: 72,425,745
effective HSP length: 52
effective length of database: 53,917,645
effective search space used: 9974764325
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)