TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QAH4a02.xg.2.1
(526 letters)
Database: Pinus_nucl_with_EST.fasta
355,925 sequences; 217,277,237 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|DR090575.1|DR090575 RTAL1_16_B07.b1_A029 Roots plus adde... 152 3e-046
gb|DR090649.1|DR090649 RTAL1_16_B07.g1_A029 Roots plus adde... 152 3e-046
gb|CO366343.1|CO366343 RTK1_27_G06.b1_A029 Roots minus pota... 150 1e-045
gb|CO363686.1|CO363686 RTK1_11_F01.b1_A029 Roots minus pota... 123 1e-037
gb|DR743131.1|DR743131 RTCU1_13_F08.g1_A029 Roots plus adde... 122 2e-037
gb|DR119509.1|DR119509 RTMG1_23_E07.g1_A029 Roots minus mag... 115 3e-025
gb|AW290680.1|AW290680 NXNV044H05F Nsf Xylem Normal wood Ve... 107 7e-023
gb|CD027338.1|CD027338 NXNV044H05 Nsf Xylem Normal wood Ver... 107 7e-023
>gb|DR090575.1|DR090575 RTAL1_16_B07.b1_A029 Roots plus added aluminum Pinus taeda cDNA
clone RTAL1_16_B07_A029 3', mRNA sequence
Length = 778
Score = 152 bits (328), Expect(2) = 3e-046
Identities = 61/105 (58%), Positives = 79/105 (75%)
Frame = +1 / +1
Query: 58 NSDVVEMASCAPLLVNDNDQRWNPDAIVFNSWQHYGCPNYWMLHFFKDSSSATLHPSTIQ 237
NSDVVEMAS APL VN N++RWNPDAIVF+SWQ YG P+YW+ FF++SS A L P TIQ
Sbjct: 67 NSDVVEMASYAPLFVNVNNRRWNPDAIVFDSWQQYGTPSYWVQQFFRESSGAVLLPVTIQ 246
Query: 238 LPNYDQLVTSAITWNNPHDGNTYMKIKVVNFGSKAVSLNISVTGL 372
LV SAI W++ DG Y+++K VNFGS ++L+I+++GL
Sbjct: 247 ENATSALVASAIRWSSLEDGEDYIRVKAVNFGSSLLTLDITISGL 381
Score = 123 bits (265), Expect(2) = 4e-033
Identities = 64/127 (50%), Positives = 77/127 (60%)
Frame = -3 / -3
Query: 383 ISVSNPVTDIFRLTALLPKFTTFIFM*VFPSCGLFQVIADVTSWS*FGS*IVDG*SVALL 204
+ P+ + ++ LPKFT F M* PS L +IA+ T S IV G S A L
Sbjct: 392 LPTGKPLIVMSSVSRELPKFTAFTLM*SSPSSKLLHLIAEATKADVAFSCIVTGRSTAPL 213
Query: 203 ESLKKCSIQ*FGHP*CCHELKTIASGFHLWSLSFTRSGAQLAISTTSLFLSNPMRNAASA 24
+SLK C Q* G P CCHE TIASGFHL L+ T +GA LAISTTSLF+SNP+R AS
Sbjct: 212 DSLKNCCTQ*LGVPYCCHESNTIASGFHLRLLTLTNNGA*LAISTTSLFVSNPIRKPASP 33
Query: 23 KAAISVP 3
AA +P
Sbjct: 32 IAANKLP 12
Score = 52.6 bits (109), Expect(2) = 3e-046
Identities = 22/46 (47%), Positives = 30/46 (65%)
Frame = +1 / +1
Query: 382 IQTFGSIKTVLTSGWLRDENSFQQPDKVVPAASPITNAGKQMGVIL 519
I++ GS KT+LTS L DENSF P K+ P A+ I N+G + + L
Sbjct: 394 IRSSGSKKTILTSDNLMDENSFDMPQKIAPKATEIVNSGTDINISL 531
Score = 36.7 bits (74), Expect(2) = 4e-033
Identities = 17/38 (44%), Positives = 20/38 (52%)
Frame = -3 / -3
Query: 506 ICLPALVIGLAAGTTLSGC*KEFSSRSQPDVRTVFMDP 393
I +P I +A G G KEFSS DVR VF +P
Sbjct: 518 ISVPLFTISVALGAIFCGMSKEFSSIKLSDVRMVFFEP 405
>gb|DR090649.1|DR090649 RTAL1_16_B07.g1_A029 Roots plus added aluminum Pinus taeda cDNA
clone RTAL1_16_B07_A029 5', mRNA sequence
Length = 746
Score = 152 bits (328), Expect(2) = 3e-046
Identities = 61/105 (58%), Positives = 79/105 (75%)
Frame = +1 / +3
Query: 58 NSDVVEMASCAPLLVNDNDQRWNPDAIVFNSWQHYGCPNYWMLHFFKDSSSATLHPSTIQ 237
NSDVVEMAS APL VN N++RWNPDAIVF+SWQ YG P+YW+ FF++SS A L P TIQ
Sbjct: 66 NSDVVEMASYAPLFVNVNNRRWNPDAIVFDSWQQYGTPSYWVQQFFRESSGAVLLPVTIQ 245
Query: 238 LPNYDQLVTSAITWNNPHDGNTYMKIKVVNFGSKAVSLNISVTGL 372
LV SAI W++ DG Y+++K VNFGS ++L+I+++GL
Sbjct: 246 ENATSALVASAIRWSSLEDGEDYIRVKAVNFGSSLLTLDITISGL 380
Score = 123 bits (265), Expect(2) = 4e-033
Identities = 64/127 (50%), Positives = 77/127 (60%)
Frame = -3 / -2
Query: 383 ISVSNPVTDIFRLTALLPKFTTFIFM*VFPSCGLFQVIADVTSWS*FGS*IVDG*SVALL 204
+ P+ + ++ LPKFT F M* PS L +IA+ T S IV G S A L
Sbjct: 391 LPTGKPLIVMSSVSRELPKFTAFTLM*SSPSSKLLHLIAEATKADVAFSCIVTGRSTAPL 212
Query: 203 ESLKKCSIQ*FGHP*CCHELKTIASGFHLWSLSFTRSGAQLAISTTSLFLSNPMRNAASA 24
+SLK C Q* G P CCHE TIASGFHL L+ T +GA LAISTTSLF+SNP+R AS
Sbjct: 211 DSLKNCCTQ*LGVPYCCHESNTIASGFHLRLLTLTNNGA*LAISTTSLFVSNPIRKPASP 32
Query: 23 KAAISVP 3
AA +P
Sbjct: 31 IAANKLP 11
Score = 52.6 bits (109), Expect(2) = 3e-046
Identities = 22/46 (47%), Positives = 30/46 (65%)
Frame = +1 / +3
Query: 382 IQTFGSIKTVLTSGWLRDENSFQQPDKVVPAASPITNAGKQMGVIL 519
I++ GS KT+LTS L DENSF P K+ P A+ I N+G + + L
Sbjct: 393 IRSSGSKKTILTSDNLMDENSFDMPQKIAPKATEIVNSGTDINISL 530
Score = 36.7 bits (74), Expect(2) = 4e-033
Identities = 17/38 (44%), Positives = 20/38 (52%)
Frame = -3 / -2
Query: 506 ICLPALVIGLAAGTTLSGC*KEFSSRSQPDVRTVFMDP 393
I +P I +A G G KEFSS DVR VF +P
Sbjct: 517 ISVPLFTISVALGAIFCGMSKEFSSIKLSDVRMVFFEP 404
>gb|CO366343.1|CO366343 RTK1_27_G06.b1_A029 Roots minus potassium Pinus taeda cDNA clone
RTK1_27_G06_A029 3', mRNA sequence
Length = 884
Score = 150 bits (324), Expect(2) = 1e-045
Identities = 60/105 (57%), Positives = 79/105 (75%)
Frame = +1 / +2
Query: 58 NSDVVEMASCAPLLVNDNDQRWNPDAIVFNSWQHYGCPNYWMLHFFKDSSSATLHPSTIQ 237
NSDVVEMAS APL VN N++RWNPDAIVF+SWQ YG P+YW+ FF++S+ A L P TIQ
Sbjct: 5 NSDVVEMASYAPLFVNVNNRRWNPDAIVFDSWQQYGTPSYWVQQFFRESNGAVLLPVTIQ 184
Query: 238 LPNYDQLVTSAITWNNPHDGNTYMKIKVVNFGSKAVSLNISVTGL 372
LV SAI W++ DG Y+++K VNFGS ++L+I+++GL
Sbjct: 185 ENATSALVASAIRWSSLEDGEDYIRLKAVNFGSSLLTLDITISGL 319
Score = 103 bits (220), Expect(2) = 5e-027
Identities = 54/110 (49%), Positives = 65/110 (59%)
Frame = -3 / -3
Query: 383 ISVSNPVTDIFRLTALLPKFTTFIFM*VFPSCGLFQVIADVTSWS*FGS*IVDG*SVALL 204
+ P+ + ++ LPKFT F M* PS L +IA+ T S IV G S A
Sbjct: 330 LPTGKPLIVMSSVSRELPKFTAFNLM*SSPSSKLLHLIAEATKADVAFSCIVTGRSTAPF 151
Query: 203 ESLKKCSIQ*FGHP*CCHELKTIASGFHLWSLSFTRSGAQLAISTTSLFL 54
+SLK C Q* G P CCHE TIASGFHL L+ T +GA LAISTTSLF+
Sbjct: 150 DSLKNCCTQ*LGVPYCCHESNTIASGFHLRLLTLTNNGA*LAISTTSLFV 1
Score = 52.6 bits (109), Expect(2) = 1e-045
Identities = 22/46 (47%), Positives = 30/46 (65%)
Frame = +1 / +2
Query: 382 IQTFGSIKTVLTSGWLRDENSFQQPDKVVPAASPITNAGKQMGVIL 519
I++ GS KT+LTS L DENSF P K+ P A+ I N+G + + L
Sbjct: 332 IRSSGSKKTILTSDNLMDENSFDMPQKIAPKATEIVNSGTDINISL 469
Score = 36.7 bits (74), Expect(2) = 5e-027
Identities = 17/38 (44%), Positives = 20/38 (52%)
Frame = -3 / -3
Query: 506 ICLPALVIGLAAGTTLSGC*KEFSSRSQPDVRTVFMDP 393
I +P I +A G G KEFSS DVR VF +P
Sbjct: 456 ISVPLFTISVALGAIFCGMSKEFSSIKLSDVRMVFFEP 343
>gb|CO363686.1|CO363686 RTK1_11_F01.b1_A029 Roots minus potassium Pinus taeda cDNA clone
RTK1_11_F01_A029 3', mRNA sequence
Length = 836
Score = 123 bits (265), Expect(2) = 1e-037
Identities = 47/90 (52%), Positives = 66/90 (73%)
Frame = +1 / +2
Query: 103 NDNDQRWNPDAIVFNSWQHYGCPNYWMLHFFKDSSSATLHPSTIQLPNYDQLVTSAITWN 282
N N++RWNPDAIVF+SWQ YG P+YW+ FF++S+ A L P TIQ LV SAI W+
Sbjct: 2 NVNNRRWNPDAIVFDSWQQYGTPSYWVQQFFRESNGAVLLPVTIQENATSALVASAIRWS 181
Query: 283 NPHDGNTYMKIKVVNFGSKAVSLNISVTGL 372
+ DG Y+++K VNFGS ++L+I+++GL
Sbjct: 182 SLEDGEDYIRLKAVNFGSSLLTLDITISGL 271
Score = 52.6 bits (109), Expect(2) = 1e-037
Identities = 22/46 (47%), Positives = 30/46 (65%)
Frame = +1 / +2
Query: 382 IQTFGSIKTVLTSGWLRDENSFQQPDKVVPAASPITNAGKQMGVIL 519
I++ GS KT+LTS L DENSF P K+ P A+ I N+G + + L
Sbjct: 284 IRSSGSKKTILTSDNLMDENSFDMPQKIAPKATEIVNSGTDINISL 421
>gb|DR743131.1|DR743131 RTCU1_13_F08.g1_A029 Roots plus added copper Pinus taeda cDNA clone
RTCU1_13_F08_A029 5', mRNA sequence
Length = 760
Score = 122 bits (263), Expect(2) = 2e-037
Identities = 46/88 (52%), Positives = 65/88 (73%)
Frame = +1 / +2
Query: 109 NDQRWNPDAIVFNSWQHYGCPNYWMLHFFKDSSSATLHPSTIQLPNYDQLVTSAITWNNP 288
N++RWNPDAIVF+SWQ YG P+YW+ FF++S+ A L P TIQ LV SAI W++
Sbjct: 17 NNRRWNPDAIVFDSWQQYGTPSYWVQQFFRESNGAVLLPVTIQENATSALVASAIRWSSL 196
Query: 289 HDGNTYMKIKVVNFGSKAVSLNISVTGL 372
DG Y+++K VNFGS ++L+I+++GL
Sbjct: 197 EDGEDYIRVKAVNFGSSLLTLDITISGL 280
Score = 52.6 bits (109), Expect(2) = 2e-037
Identities = 22/46 (47%), Positives = 30/46 (65%)
Frame = +1 / +2
Query: 382 IQTFGSIKTVLTSGWLRDENSFQQPDKVVPAASPITNAGKQMGVIL 519
I++ GS KT+LTS L DENSF P K+ P A+ I N+G + + L
Sbjct: 293 IRSSGSKKTILTSDNLMDENSFDMPQKIAPKATEIVNSGTDINISL 430
>gb|DR119509.1|DR119509 RTMG1_23_E07.g1_A029 Roots minus magnesium Pinus taeda cDNA clone
RTMG1_23_E07_A029 5', mRNA sequence
Length = 619
Score = 115 bits (246), Expect = 3e-025
Identities = 47/74 (63%), Positives = 55/74 (74%)
Frame = +1 / +3
Query: 58 NSDVVEMASCAPLLVNDNDQRWNPDAIVFNSWQHYGCPNYWMLHFFKDSSSATLHPSTIQ 237
NSDVVEMAS APL VN N++RWNPDAIVF+SWQ YG P+YW+ FF++S+ A L P TIQ
Sbjct: 396 NSDVVEMASYAPLFVNVNNRRWNPDAIVFDSWQQYGTPSYWVQQFFRESNGAVLLPVTIQ 575
Query: 238 LPNYDQLVTSAITW 279
LV SAI W
Sbjct: 576 ENATSALVASAIRW 617
Score = 100 bits (214), Expect = 6e-021
Identities = 51/91 (56%), Positives = 59/91 (64%)
Frame = -3 / -1
Query: 275 VIADVTSWS*FGS*IVDG*SVALLESLKKCSIQ*FGHP*CCHELKTIASGFHLWSLSFTR 96
+IA+ T S IV G S A +SLK C Q* G P CCHE TIASGFHL L+ T
Sbjct: 613 LIAEATKADVAFSCIVTGRSTAPFDSLKNCCTQ*LGVPYCCHESNTIASGFHLRLLTLTN 434
Query: 95 SGAQLAISTTSLFLSNPMRNAASAKAAISVP 3
+GA LAISTTSLF+SNP+R AS AA +P
Sbjct: 433 NGA*LAISTTSLFVSNPIRKPASPIAANKLP 341
>gb|AW290680.1|AW290680 NXNV044H05F Nsf Xylem Normal wood Vertical Pinus taeda cDNA clone
NXNV044H05 5', mRNA sequence
Length = 335
Score = 107 bits (229), Expect = 7e-023
Identities = 42/60 (70%), Positives = 49/60 (81%)
Frame = +1 / +2
Query: 58 NSDVVEMASCAPLLVNDNDQRWNPDAIVFNSWQHYGCPNYWMLHFFKDSSSATLHPSTIQ 237
NSDVVEMAS APL VN N++RWNPDAIVF+SWQ YG P+YW+ FF++SS A L P TIQ
Sbjct: 107 NSDVVEMASYAPLFVNVNNRRWNPDAIVFDSWQQYGTPSYWVQQFFRESSGAVLLPVTIQ 286
>gb|CD027338.1|CD027338 NXNV044H05 Nsf Xylem Normal wood Vertical Pinus taeda cDNA clone
NXNV044H05 5' similar to Arabidopsis thaliana sequence
At3g10740 putative alpha-L-arabinofuranosidase see
http://mips.gsf.de/proj/thal/db/index.html, mRNA
sequence
Length = 335
Score = 107 bits (229), Expect = 7e-023
Identities = 42/60 (70%), Positives = 49/60 (81%)
Frame = +1 / +2
Query: 58 NSDVVEMASCAPLLVNDNDQRWNPDAIVFNSWQHYGCPNYWMLHFFKDSSSATLHPSTIQ 237
NSDVVEMAS APL VN N++RWNPDAIVF+SWQ YG P+YW+ FF++SS A L P TIQ
Sbjct: 107 NSDVVEMASYAPLFVNVNNRRWNPDAIVFDSWQQYGTPSYWVQQFFRESSGAVLLPVTIQ 286
Database: Pinus_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:45 PM
Number of letters in database: 217,277,237
Number of sequences in database: 355,925
Lambda K H
0.316 0.130 0.405
Matrix: BLOSUM62
Number of Hits to DB: 329,587,964
Number of Sequences: 355925
Number of extensions: 4985181
Number of successful extensions: 58727
Number of sequences better than 1.0e-020: 10
length of database: 72,425,745
effective HSP length: 50
effective length of database: 54,629,495
effective search space used: 6774057380
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.6 bits)