TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QAF14f01.yg.2.1
(319 letters)
Database: Pinus_nucl_with_EST.fasta
355,925 sequences; 217,277,237 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CX713265.1|CX713265 RTPQ1_8_B07.b1_A032 Roots treated wi... 73 1e-024
gb|DR169090.1|DR169090 RTPHOS1_30_A06.b1_A029 Roots minus p... 73 1e-024
gb|DR014696.1|DR014696 HEAT1_51_C03.b1_A029 Root at 37 C fo... 73 2e-024
gb|DR689244.1|DR689244 EST1079330 Normalized pine embryo li... 73 2e-024
gb|DR109571.1|DR109571 RTS1_3_E05.b1_A029 Roots minus sulfu... 73 2e-024
gb|DT635347.1|DT635347 EST1150278 Normalized pine embryo li... 73 2e-024
>gb|CX713265.1|CX713265 RTPQ1_8_B07.b1_A032 Roots treated with paraquat Pinus taeda cDNA
clone RTPQ1_8_B07_A032 3', mRNA sequence
Length = 702
Score = 73.5 bits (154), Expect(2) = 1e-024
Identities = 24/54 (44%), Positives = 34/54 (62%)
Frame = -3 / +2
Query: 317 YLXXXXPIVRDRFNPNACAWAYGVNVFXLXXXXRDGCTELFHQYMEMNEDXELW 156
Y+ P+++ RFNP AC WAYG+N F L R+ CTE +H + +NE+ LW
Sbjct: 11 YMNFSHPLIKARFNPKACGWAYGMNFFHLDAWRREKCTEEYHYWQNLNENRTLW 172
Score = 57.0 bits (118), Expect(2) = 1e-024
Identities = 24/40 (60%), Positives = 27/40 (67%)
Frame = -3 / +2
Query: 140 LTAGXXXXXGNTKXXDKSWHVMGLGYNPSISPEAIRSAAV 21
L G TK DKSWHV+GLGYNPSIS + IR+AAV
Sbjct: 185 LPPGLITYYKTTKPLDKSWHVLGLGYNPSISMDEIRNAAV 304
>gb|DR169090.1|DR169090 RTPHOS1_30_A06.b1_A029 Roots minus phosphorous Pinus taeda cDNA
clone RTPHOS1_30_A06_A029 3', mRNA sequence
Length = 710
Score = 73.5 bits (154), Expect(2) = 1e-024
Identities = 24/54 (44%), Positives = 34/54 (62%)
Frame = -3 / +3
Query: 317 YLXXXXPIVRDRFNPNACAWAYGVNVFXLXXXXRDGCTELFHQYMEMNEDXELW 156
Y+ P+++ RFNP AC WAYG+N F L R+ CTE +H + +NE+ LW
Sbjct: 36 YMNFSHPLIKARFNPKACGWAYGMNFFHLDAWRREKCTEEYHYWQNLNENRTLW 197
Score = 57.0 bits (118), Expect(2) = 1e-024
Identities = 24/40 (60%), Positives = 27/40 (67%)
Frame = -3 / +3
Query: 140 LTAGXXXXXGNTKXXDKSWHVMGLGYNPSISPEAIRSAAV 21
L G TK DKSWHV+GLGYNPSIS + IR+AAV
Sbjct: 210 LPPGLITYYKTTKPLDKSWHVLGLGYNPSISMDEIRNAAV 329
>gb|DR014696.1|DR014696 HEAT1_51_C03.b1_A029 Root at 37 C for 24 hr Pinus taeda cDNA clone
HEAT1_51_C03_A029 3', mRNA sequence
Length = 914
Score = 73.5 bits (154), Expect(2) = 2e-024
Identities = 24/54 (44%), Positives = 34/54 (62%)
Frame = -3 / +2
Query: 317 YLXXXXPIVRDRFNPNACAWAYGVNVFXLXXXXRDGCTELFHQYMEMNEDXELW 156
Y+ P+++ RFNP AC WAYG+N F L R+ CTE +H + +NE+ LW
Sbjct: 89 YMNFSHPLIKARFNPKACGWAYGMNFFHLDAWRREKCTEEYHYWQNLNENRTLW 250
Score = 57.0 bits (118), Expect(2) = 2e-024
Identities = 24/40 (60%), Positives = 27/40 (67%)
Frame = -3 / +2
Query: 140 LTAGXXXXXGNTKXXDKSWHVMGLGYNPSISPEAIRSAAV 21
L G TK DKSWHV+GLGYNPSIS + IR+AAV
Sbjct: 263 LPPGLITYYKTTKPLDKSWHVLGLGYNPSISMDEIRNAAV 382
>gb|DR689244.1|DR689244 EST1079330 Normalized pine embryo library, Lib_D Pinus taeda cDNA
clone PWACF91 3' end, mRNA sequence
Length = 854
Score = 73.5 bits (154), Expect(2) = 2e-024
Identities = 24/54 (44%), Positives = 34/54 (62%)
Frame = -3 / +2
Query: 317 YLXXXXPIVRDRFNPNACAWAYGVNVFXLXXXXRDGCTELFHQYMEMNEDXELW 156
Y+ P+++ RFNP AC WAYG+N F L R+ CTE +H + +NE+ LW
Sbjct: 209 YMNFSHPLIKARFNPKACGWAYGMNFFHLDAWRREKCTEEYHYWQNLNENRTLW 370
Score = 57.0 bits (118), Expect(2) = 2e-024
Identities = 24/40 (60%), Positives = 27/40 (67%)
Frame = -3 / +2
Query: 140 LTAGXXXXXGNTKXXDKSWHVMGLGYNPSISPEAIRSAAV 21
L G TK DKSWHV+GLGYNPSIS + IR+AAV
Sbjct: 383 LPPGLITYYKTTKPLDKSWHVLGLGYNPSISMDEIRNAAV 502
>gb|DR109571.1|DR109571 RTS1_3_E05.b1_A029 Roots minus sulfur Pinus taeda cDNA clone
RTS1_3_E05_A029 3', mRNA sequence
Length = 820
Score = 73.5 bits (154), Expect(2) = 2e-024
Identities = 24/54 (44%), Positives = 34/54 (62%)
Frame = -3 / +3
Query: 317 YLXXXXPIVRDRFNPNACAWAYGVNVFXLXXXXRDGCTELFHQYMEMNEDXELW 156
Y+ P+++ RFNP AC WAYG+N F L R+ CTE +H + +NE+ LW
Sbjct: 225 YMNFSHPLIKARFNPKACGWAYGMNFFHLDAWRREKCTEEYHYWQNLNENRTLW 386
Score = 57.0 bits (118), Expect(2) = 2e-024
Identities = 24/40 (60%), Positives = 27/40 (67%)
Frame = -3 / +3
Query: 140 LTAGXXXXXGNTKXXDKSWHVMGLGYNPSISPEAIRSAAV 21
L G TK DKSWHV+GLGYNPSIS + IR+AAV
Sbjct: 399 LPPGLITYYKTTKPLDKSWHVLGLGYNPSISMDEIRNAAV 518
>gb|DT635347.1|DT635347 EST1150278 Normalized pine embryo library, Lib_D Pinus taeda cDNA
clone PIMGF84 3' end, mRNA sequence
Length = 768
Score = 73.5 bits (154), Expect(2) = 2e-024
Identities = 24/54 (44%), Positives = 34/54 (62%)
Frame = -3 / +1
Query: 317 YLXXXXPIVRDRFNPNACAWAYGVNVFXLXXXXRDGCTELFHQYMEMNEDXELW 156
Y+ P+++ RFNP AC WAYG+N F L R+ CTE +H + +NE+ LW
Sbjct: 217 YMNFSHPLIKARFNPKACGWAYGMNFFHLDAWRREKCTEEYHYWQNLNENRTLW 378
Score = 57.0 bits (118), Expect(2) = 2e-024
Identities = 24/40 (60%), Positives = 27/40 (67%)
Frame = -3 / +1
Query: 140 LTAGXXXXXGNTKXXDKSWHVMGLGYNPSISPEAIRSAAV 21
L G TK DKSWHV+GLGYNPSIS + IR+AAV
Sbjct: 391 LPPGLITYYKTTKPLDKSWHVLGLGYNPSISMDEIRNAAV 510
Database: Pinus_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:45 PM
Number of letters in database: 217,277,237
Number of sequences in database: 355,925
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 156,973,814
Number of Sequences: 355925
Number of extensions: 1650641
Number of successful extensions: 86592
Number of sequences better than 1.0e-020: 6
length of database: 72,425,745
effective HSP length: 49
effective length of database: 54,985,420
effective search space used: 3079183520
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)