TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 8161196.3.1
(825 letters)
Database: Pinus_nucl_with_EST.fasta
355,925 sequences; 217,277,237 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|DT626887.1|DT626887 EST1159963 Sequencing ESTs from lobl... 117 5e-033
gb|DN461002.1|DN461002 EST956801 Sequencing ESTs from loblo... 117 5e-033
gb|DN614955.1|DN614955 EST968005 Subtracted pine embryo lib... 117 5e-033
gb|DN614956.1|DN614956 EST968006 Subtracted pine embryo lib... 117 5e-033
gb|DR068879.1|DR068879 RTDK1_3_A06.g1_A029 Roots, dark Pinu... 115 2e-028
>gb|DT626887.1|DT626887 EST1159963 Sequencing ESTs from loblolly pine embryos Pinus taeda
cDNA clone PIMAS51 5' end, mRNA sequence
Length = 960
Score = 117 bits (251), Expect(2) = 5e-033
Identities = 43/79 (54%), Positives = 53/79 (67%)
Frame = +3 / +3
Query: 366 MVPLCGFCGKQRSMIYCRSDAASLCLSCDRSVHSANALSRRHRRTLLCDRCGLQPASVRC 545
M +C FCG+ RS +YC++DAASLCL CD +H ANALS+RH RTLLCD C ++PA+ C
Sbjct: 246 MGTMCDFCGEIRSTVYCKADAASLCLPCDGQIHGANALSKRHLRTLLCDGCSVEPAAFSC 425
Query: 546 LEDNTSLCQNCDWNGHDAA 602
S C NCD H A
Sbjct: 426 NNHKLSFCHNCDRQSHSNA 482
Score = 43.7 bits (89), Expect(2) = 5e-033
Identities = 15/28 (53%), Positives = 22/28 (78%)
Frame = +3 / +3
Query: 603 SGASGHKRQAINCYSGCPSSAELSRIWS 686
S A HKR++I+ Y+GCPS AEL+ +W+
Sbjct: 474 SNAPQHKRKSISYYTGCPSPAELAELWN 557
>gb|DN461002.1|DN461002 EST956801 Sequencing ESTs from loblolly pine embryos Pinus taeda
cDNA clone RPII516 5' end, mRNA sequence
Length = 940
Score = 117 bits (251), Expect(2) = 5e-033
Identities = 43/79 (54%), Positives = 53/79 (67%)
Frame = +3 / +1
Query: 366 MVPLCGFCGKQRSMIYCRSDAASLCLSCDRSVHSANALSRRHRRTLLCDRCGLQPASVRC 545
M +C FCG+ RS +YC++DAASLCL CD +H ANALS+RH RTLLCD C ++PA+ C
Sbjct: 247 MGTMCDFCGEIRSTVYCKADAASLCLPCDGQIHGANALSKRHLRTLLCDGCSVEPAAFSC 426
Query: 546 LEDNTSLCQNCDWNGHDAA 602
S C NCD H A
Sbjct: 427 NNHKLSFCHNCDRQSHSNA 483
Score = 43.7 bits (89), Expect(2) = 5e-033
Identities = 15/28 (53%), Positives = 22/28 (78%)
Frame = +3 / +1
Query: 603 SGASGHKRQAINCYSGCPSSAELSRIWS 686
S A HKR++I+ Y+GCPS AEL+ +W+
Sbjct: 475 SNAPQHKRKSISYYTGCPSPAELAELWN 558
>gb|DN614955.1|DN614955 EST968005 Subtracted pine embryo library, Lib_B Pinus taeda cDNA
clone PSACA13, mRNA sequence
Length = 605
Score = 117 bits (251), Expect(2) = 5e-033
Identities = 43/79 (54%), Positives = 53/79 (67%)
Frame = +3 / +3
Query: 366 MVPLCGFCGKQRSMIYCRSDAASLCLSCDRSVHSANALSRRHRRTLLCDRCGLQPASVRC 545
M +C FCG+ RS +YC++DAASLCL CD +H ANALS+RH RTLLCD C ++PA+ C
Sbjct: 45 MGTMCDFCGEIRSTVYCKADAASLCLPCDGQIHGANALSKRHLRTLLCDGCSVEPAAFSC 224
Query: 546 LEDNTSLCQNCDWNGHDAA 602
S C NCD H A
Sbjct: 225 NNHKLSFCHNCDRQSHSNA 281
Score = 43.7 bits (89), Expect(2) = 5e-033
Identities = 15/28 (53%), Positives = 22/28 (78%)
Frame = +3 / +3
Query: 603 SGASGHKRQAINCYSGCPSSAELSRIWS 686
S A HKR++I+ Y+GCPS AEL+ +W+
Sbjct: 273 SNAPQHKRKSISYYTGCPSPAELAELWN 356
>gb|DN614956.1|DN614956 EST968006 Subtracted pine embryo library, Lib_B Pinus taeda cDNA
clone PSACA14, mRNA sequence
Length = 580
Score = 117 bits (251), Expect(2) = 5e-033
Identities = 43/79 (54%), Positives = 53/79 (67%)
Frame = +3 / +2
Query: 366 MVPLCGFCGKQRSMIYCRSDAASLCLSCDRSVHSANALSRRHRRTLLCDRCGLQPASVRC 545
M +C FCG+ RS +YC++DAASLCL CD +H ANALS+RH RTLLCD C ++PA+ C
Sbjct: 20 MGTMCDFCGEIRSTVYCKADAASLCLPCDGQIHGANALSKRHLRTLLCDGCSVEPAAFSC 199
Query: 546 LEDNTSLCQNCDWNGHDAA 602
S C NCD H A
Sbjct: 200 NNHKLSFCHNCDRQSHSNA 256
Score = 43.7 bits (89), Expect(2) = 5e-033
Identities = 15/28 (53%), Positives = 22/28 (78%)
Frame = +3 / +2
Query: 603 SGASGHKRQAINCYSGCPSSAELSRIWS 686
S A HKR++I+ Y+GCPS AEL+ +W+
Sbjct: 248 SNAPQHKRKSISYYTGCPSPAELAELWN 331
>gb|DR068879.1|DR068879 RTDK1_3_A06.g1_A029 Roots, dark Pinus taeda cDNA clone
RTDK1_3_A06_A029 5', mRNA sequence
Length = 828
Score = 115 bits (245), Expect(2) = 2e-028
Identities = 41/76 (53%), Positives = 53/76 (69%)
Frame = +3 / +2
Query: 378 CGFCGKQRSMIYCRSDAASLCLSCDRSVHSANALSRRHRRTLLCDRCGLQPASVRCLEDN 557
C +CG+ ++IYCR+D+A LCL CD VHSANALS++H R+ LCD C +P V C D+
Sbjct: 221 CDYCGEANAVIYCRADSAKLCLPCDHHVHSANALSKKHVRSQLCDSCKDKPVIVYCAADD 400
Query: 558 TSLCQNCDWNGHDAAS 605
LCQ CDW+ H AS
Sbjct: 401 AVLCQECDWDTHGGAS 448
Score = 30.9 bits (61), Expect(2) = 2e-028
Identities = 10/24 (41%), Positives = 16/24 (66%)
Frame = +3 / +2
Query: 618 HKRQAINCYSGCPSSAELSRIWSF 689
H+R +I ++GCPS+ L+ W F
Sbjct: 458 HQRHSIEGFTGCPSAEILATKWGF 529
Database: Pinus_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:45 PM
Number of letters in database: 217,277,237
Number of sequences in database: 355,925
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 479,674,029
Number of Sequences: 355925
Number of extensions: 7475665
Number of successful extensions: 71298
Number of sequences better than 1.0e-020: 5
length of database: 72,425,745
effective HSP length: 52
effective length of database: 53,917,645
effective search space used: 11969717190
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)