TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 5704580.2.1
(721 letters)
Database: Pinus_nucl_with_EST.fasta
355,925 sequences; 217,277,237 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|DR055191.1|DR055191 RTCA1_22_D09.b1_A029 Roots minus cal... 237 9e-062
gb|DR055276.1|DR055276 RTCA1_22_D09.g2_A029 Roots minus cal... 237 9e-062
gb|CX645415.1|CX645415 COLD1_2_D04.g1_A029 Root cold Pinus ... 169 2e-049
>gb|DR055191.1|DR055191 RTCA1_22_D09.b1_A029 Roots minus calcium Pinus taeda cDNA clone
RTCA1_22_D09_A029 3', mRNA sequence
Length = 773
Score = 237 bits (511), Expect = 9e-062
Identities = 90/140 (64%), Positives = 124/140 (88%)
Frame = -3 / +2
Query: 692 VEACIEETIVVYVDHLLSQKNYIKEETIERMRLDEEVLMDFFREHINVTKVESRVRILAD 513
VEAC+EE +VVY+DHLL+QKNYIKE+T+ERMR+DEEV +DFFR++I+++KVE RV+ LAD
Sbjct: 35 VEACLEEAVVVYIDHLLTQKNYIKEKTLERMRVDEEVFLDFFRDYIDLSKVEKRVQPLAD 214
Query: 512 MRELASAESLDSFTLIYTNILEHQPDCPPEVFYKLVAMREGIPKKEAKEVVQECKEIYEN 333
+RELASAES++ FTL+Y+NIL++ PDCPPEV K++A+REGIP+K+A+E++QECK+IY
Sbjct: 215 LRELASAESVEFFTLVYSNILQNHPDCPPEVVEKVIALREGIPRKDAREILQECKDIYNT 394
Query: 332 SLVDGNPQKSGFVFGKLKCL 273
SL +G+ K GF+F +L CL
Sbjct: 395 SLDNGDLPKPGFLFARLTCL 454
Score = 116 bits (247), Expect = 2e-025
Identities = 53/134 (39%), Positives = 80/134 (59%)
Frame = +3 / -2
Query: 291 KHKTGLLRVAIHKRVLVYLFAFLYNLLGFLFWNAFPHCHKLIKNLRRAIRLMFQYISVNE 470
K K+ L ++I KR ++Y+ AFL L G L W +F C+ L+ + RR IR++ + + V++
Sbjct: 436 KEKSRLG*ISIIKRSVIYVLAFL*YLTGILPWYSFS*CNDLLYDFRRTIRVVLENV*VDK 257
Query: 471 SKAIQTLS*S*LPHICQNPHSAFNFCHVDVFPEEIHKHLFI*PHPLYRFFFYVVFLRKEM 650
SK TLS S ICQ HS F +++ PE+I ++ + HP F VVFL KEM
Sbjct: 256 SKKFHTLSRSKFSQICQRLHSFLYFR*INIIPEKIQEYFLVNTHPFKCFLLDVVFLSKEM 77
Query: 651 VNIDHNCFLYASLH 692
+N++HNC AS +
Sbjct: 76 INVNHNCLF*ASFN 35
>gb|DR055276.1|DR055276 RTCA1_22_D09.g2_A029 Roots minus calcium Pinus taeda cDNA clone
RTCA1_22_D09_A029 5', mRNA sequence
Length = 565
Score = 237 bits (511), Expect = 9e-062
Identities = 90/140 (64%), Positives = 124/140 (88%)
Frame = -3 / +3
Query: 692 VEACIEETIVVYVDHLLSQKNYIKEETIERMRLDEEVLMDFFREHINVTKVESRVRILAD 513
VEAC+EE +VVY+DHLL+QKNYIKE+T+ERMR+DEEV +DFFR++I+++KVE RV+ LAD
Sbjct: 144 VEACLEEAVVVYIDHLLTQKNYIKEKTLERMRVDEEVFLDFFRDYIDLSKVEKRVQPLAD 323
Query: 512 MRELASAESLDSFTLIYTNILEHQPDCPPEVFYKLVAMREGIPKKEAKEVVQECKEIYEN 333
+RELASAES++ FTL+Y+NIL++ PDCPPEV K++A+REGIP+K+A+E++QECK+IY
Sbjct: 324 LRELASAESVEFFTLVYSNILQNHPDCPPEVVEKVIALREGIPRKDAREILQECKDIYNT 503
Query: 332 SLVDGNPQKSGFVFGKLKCL 273
SL +G+ K GF+F +L CL
Sbjct: 504 SLDNGDLPKPGFLFARLTCL 563
Score = 116 bits (247), Expect = 2e-025
Identities = 53/134 (39%), Positives = 80/134 (59%)
Frame = +3 / -3
Query: 291 KHKTGLLRVAIHKRVLVYLFAFLYNLLGFLFWNAFPHCHKLIKNLRRAIRLMFQYISVNE 470
K K+ L ++I KR ++Y+ AFL L G L W +F C+ L+ + RR IR++ + + V++
Sbjct: 545 KEKSRLG*ISIIKRSVIYVLAFL*YLTGILPWYSFS*CNDLLYDFRRTIRVVLENV*VDK 366
Query: 471 SKAIQTLS*S*LPHICQNPHSAFNFCHVDVFPEEIHKHLFI*PHPLYRFFFYVVFLRKEM 650
SK TLS S ICQ HS F +++ PE+I ++ + HP F VVFL KEM
Sbjct: 365 SKKFHTLSRSKFSQICQRLHSFLYFR*INIIPEKIQEYFLVNTHPFKCFLLDVVFLSKEM 186
Query: 651 VNIDHNCFLYASLH 692
+N++HNC AS +
Sbjct: 185 INVNHNCLF*ASFN 144
>gb|CX645415.1|CX645415 COLD1_2_D04.g1_A029 Root cold Pinus taeda cDNA clone
COLD1_2_D04_A029 5', mRNA sequence
Length = 641
Score = 169 bits (363), Expect(2) = 2e-049
Identities = 66/101 (65%), Positives = 87/101 (86%)
Frame = -3 / +1
Query: 572 FFREHINVTKVESRVRILADMRELASAESLDSFTLIYTNILEHQPDCPPEVFYKLVAMRE 393
FFREHIN++KVE R++ LAD+RELAS+ES++SFTL+Y+NIL++ PDCPPEV K+VA+RE
Sbjct: 67 FFREHINLSKVEKRLQPLADLRELASSESVESFTLVYSNILQNHPDCPPEVVEKIVALRE 246
Query: 392 GIPKKEAKEVVQECKEIYENSLVDGNPQKSGFVFGKLKCLT 270
GIPKK+A+EV+QECKEIY L +G+ K GF+F +L CLT
Sbjct: 247 GIPKKDAREVLQECKEIYSACLANGDLPKPGFLFARLTCLT 369
Score = 46.9 bits (96), Expect(2) = 2e-049
Identities = 17/24 (70%), Positives = 22/24 (91%)
Frame = -3 / +3
Query: 635 KNYIKEETIERMRLDEEVLMDFFR 564
KNYI+EET+ERM +DEEV +DFF+
Sbjct: 3 KNYIREETLERMLVDEEVFLDFFQ 74
Database: Pinus_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:45 PM
Number of letters in database: 217,277,237
Number of sequences in database: 355,925
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 389,169,347
Number of Sequences: 355925
Number of extensions: 5607427
Number of successful extensions: 45416
Number of sequences better than 1.0e-020: 3
length of database: 72,425,745
effective HSP length: 52
effective length of database: 53,917,645
effective search space used: 10082599615
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)