TBLASTX 2.2.10 [Oct-19-2004]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 3670899.2.1
         (555 letters)

Database: Pinus_nucl_with_EST.fasta 
           355,925 sequences; 217,277,237 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|DR080071.1|DR080071  RTFEPL1_20_B11.b1_A029 Roots plus ad...   116   2e-040
gb|DR080136.1|DR080136  RTFEPL1_20_B11.g1_A029 Roots plus ad...   116   1e-025

>gb|DR080071.1|DR080071 RTFEPL1_20_B11.b1_A029 Roots plus added iron Pinus taeda cDNA clone
           RTFEPL1_20_B11_A029 3', mRNA sequence
          Length = 753

 Score =  116 bits (248), Expect(2) = 2e-040
 Identities = 44/75 (58%), Positives = 53/75 (70%)
 Frame = -3 / +2

                                                                       
Query: 538 MYYESPTKSGEIVAVFQPSHEEQQQYTSNDHNQKALAEIYLLSYCDKITMSAWSTFGYVA 359
           MY+E PT  G++V+V+QPSHEE QQ     HN KA AE+YLLS CD +  S WSTFGYVA
Sbjct: 311 MYWEHPTLDGQLVSVYQPSHEEWQQTEKQSHNMKAWAEMYLLSLCDVLITSPWSTFGYVA 490

                          
Query: 358 YSFAGVKPWILIRPD 314
               G KPWIL +P+
Sbjct: 491 QGLGGTKPWILFKPE 535




 Score = 68.9 bits (144), Expect(2) = 2e-040
 Identities = 24/52 (46%), Positives = 33/52 (63%)
 Frame = -3 / +2

                                                               
Query: 310 NRETSDVACVRSTSVEPCLHSPPILGCRAKTDVDVAAVKPYVRHCEDVGFGL 155
           N   +D  C +  S+EPC H+PP   C+AK  +D   V PYV+HCEDV +G+
Sbjct: 536 NDTAADPPCRKVMSMEPCFHAPPFYDCQAKKGIDTGKVFPYVKHCEDVPWGI 691



>gb|DR080136.1|DR080136 RTFEPL1_20_B11.g1_A029 Roots plus added iron Pinus taeda cDNA clone
           RTFEPL1_20_B11_A029 5', mRNA sequence
          Length = 808

 Score =  116 bits (248), Expect = 1e-025
 Identities = 44/75 (58%), Positives = 53/75 (70%)
 Frame = -3 / +1

                                                                       
Query: 538 MYYESPTKSGEIVAVFQPSHEEQQQYTSNDHNQKALAEIYLLSYCDKITMSAWSTFGYVA 359
           MY+E PT  G++V+V+QPSHEE QQ     HN KA AE+YLLS CD +  S WSTFGYVA
Sbjct: 568 MYWEHPTLDGQLVSVYQPSHEEWQQTEKQSHNMKAWAEMYLLSLCDVLITSPWSTFGYVA 747

                          
Query: 358 YSFAGVKPWILIRPD 314
               G KPWIL +P+
Sbjct: 748 QGLGGTKPWILFKPE 792



  Database: Pinus_nucl_with_EST.fasta
    Posted date:  Feb 7, 2006  2:45 PM
  Number of letters in database: 217,277,237
  Number of sequences in database:  355,925
  
Lambda     K      H
   0.318    0.134    0.401 


Matrix: BLOSUM62
Number of Hits to DB: 261,587,763
Number of Sequences: 355925
Number of extensions: 3366160
Number of successful extensions: 47404
Number of sequences better than 1.0e-020: 2
length of database: 72,425,745
effective HSP length: 51
effective length of database: 54,273,570
effective search space used: 7218384810
frameshift window, decay const: 40,  0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)