TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3665525.2.1
(620 letters)
Database: Pinus_nucl_with_EST.fasta
355,925 sequences; 217,277,237 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|DR694977.1|DR694977 EST1085070 Normalized pine embryo li... 211 4e-054
gb|DT638989.1|DT638989 EST1153920 Normalized pine embryo li... 211 4e-054
gb|DR109699.1|DR109699 RTS1_4_C10.b1_A029 Roots minus sulfu... 209 1e-053
gb|BQ696389.1|BQ696389 NXPV_040_F05_F NXPV (Nsf Xylem Plani... 180 7e-045
gb|DR693981.1|DR693981 EST1084071 Normalized pine embryo li... 177 6e-044
gb|BI397792.1|BI397792 NXPV_107_E10_F NXPV (Nsf Xylem Plani... 127 9e-029
gb|CX715693.1|CX715693 RTPQ1_35_H08.g1_A032 Roots treated w... 123 9e-028
>gb|DR694977.1|DR694977 EST1085070 Normalized pine embryo library, Lib_D Pinus taeda cDNA
clone PWAEJ07 3' end, mRNA sequence
Length = 793
Score = 211 bits (455), Expect = 4e-054
Identities = 81/126 (64%), Positives = 95/126 (75%)
Frame = -2 / +1
Query: 619 IGCMKKGPVFTDPKLKWYEPQSFLLGSEYFLHAYGPIYXXXXXXXXXXXXLRNNSFRMFS 440
IGCMKKGPVF DPKLKWYEP LLGSEYF+HAYGPIY LRN+S+RMFS
Sbjct: 220 IGCMKKGPVFRDPKLKWYEPLGDLLGSEYFIHAYGPIYALSAEIVAGLSGLRNDSYRMFS 399
Query: 439 I*DVTIGSWMLAMNVNHENTHALCEPDCTESSVAVWDIPKCSGLCHPEVKMLELHQRKEC 260
DVTIG+WMLAMNVNHE+ LC P+CT +S+AVWDIPKCSGLC+P ++ ELH++ C
Sbjct: 400 NEDVTIGAWMLAMNVNHEDNRELCSPECTPTSIAVWDIPKCSGLCNPAQRLRELHKKYVC 579
Query: 259 TGGPTV 242
PT+
Sbjct: 580 MKSPTL 597
Score = 75.8 bits (159), Expect(2) = 2e-029
Identities = 34/73 (46%), Positives = 44/73 (60%)
Frame = +3 / -3
Query: 231 TSAATVGPPVHSFLXXXXXIFTSGWHSPEHFGMSQTATEDSVQSGSQSACVFSWLTFIAS 410
+S VG + ++ +G PEHFG+S TA E V SG S+ + SWLTFIAS
Sbjct: 608 SSGGNVGLFIQTYFLWSSLSLCAGLQRPEHFGISHTAIEVGVHSGEHSSRLSSWLTFIAS 429
Query: 411 IHDPIVTS*ILNI 449
IH P+VTS +LNI
Sbjct: 428 IHAPMVTSSLLNI 390
Score = 73.0 bits (153), Expect(2) = 2e-029
Identities = 30/37 (81%), Positives = 31/37 (83%)
Frame = +3 / -3
Query: 510 IGPYA*RKYSDPSKKDWGSYHFNLGSVKTGPFFMHPM 620
IGP A* KYS PSK GSYHFNLGS+KTGPFFMHPM
Sbjct: 329 IGP*A*MKYSLPSKSPKGSYHFNLGSLKTGPFFMHPM 219
>gb|DT638989.1|DT638989 EST1153920 Normalized pine embryo library, Lib_D Pinus taeda cDNA
clone PIMHL79 3' end, mRNA sequence
Length = 850
Score = 211 bits (455), Expect = 4e-054
Identities = 81/126 (64%), Positives = 95/126 (75%)
Frame = -2 / +3
Query: 619 IGCMKKGPVFTDPKLKWYEPQSFLLGSEYFLHAYGPIYXXXXXXXXXXXXLRNNSFRMFS 440
IGCMKKGPVF DPKLKWYEP LLGSEYF+HAYGPIY LRN+S+RMFS
Sbjct: 228 IGCMKKGPVFRDPKLKWYEPLGDLLGSEYFIHAYGPIYALSAEIVAGLSGLRNDSYRMFS 407
Query: 439 I*DVTIGSWMLAMNVNHENTHALCEPDCTESSVAVWDIPKCSGLCHPEVKMLELHQRKEC 260
DVTIG+WMLAMNVNHE+ LC P+CT +S+AVWDIPKCSGLC+P ++ ELH++ C
Sbjct: 408 NEDVTIGAWMLAMNVNHEDNRELCSPECTPTSIAVWDIPKCSGLCNPAQRLRELHKKYVC 587
Query: 259 TGGPTV 242
PT+
Sbjct: 588 MKSPTL 605
Score = 75.8 bits (159), Expect(2) = 2e-029
Identities = 34/73 (46%), Positives = 44/73 (60%)
Frame = +3 / -1
Query: 231 TSAATVGPPVHSFLXXXXXIFTSGWHSPEHFGMSQTATEDSVQSGSQSACVFSWLTFIAS 410
+S VG + ++ +G PEHFG+S TA E V SG S+ + SWLTFIAS
Sbjct: 616 SSGGNVGLFIQTYFLWSSLSLCAGLQRPEHFGISHTAIEVGVHSGEHSSRLSSWLTFIAS 437
Query: 411 IHDPIVTS*ILNI 449
IH P+VTS +LNI
Sbjct: 436 IHAPMVTSSLLNI 398
Score = 73.0 bits (153), Expect(2) = 2e-029
Identities = 30/37 (81%), Positives = 31/37 (83%)
Frame = +3 / -1
Query: 510 IGPYA*RKYSDPSKKDWGSYHFNLGSVKTGPFFMHPM 620
IGP A* KYS PSK GSYHFNLGS+KTGPFFMHPM
Sbjct: 337 IGP*A*MKYSLPSKSPKGSYHFNLGSLKTGPFFMHPM 227
>gb|DR109699.1|DR109699 RTS1_4_C10.b1_A029 Roots minus sulfur Pinus taeda cDNA clone
RTS1_4_C10_A029 3', mRNA sequence
Length = 808
Score = 209 bits (451), Expect = 1e-053
Identities = 80/129 (62%), Positives = 92/129 (71%)
Frame = -2 / -2
Query: 619 IGCMKKGPVFTDPKLKWYEPQSFLLGSEYFLHAYGPIYXXXXXXXXXXXXLRNNSFRMFS 440
IGCMKKGPV TDPKLKWYEP ++LLGSEYF HAYGPIY RN+SFRM S
Sbjct: 684 IGCMKKGPVITDPKLKWYEPAAYLLGSEYFYHAYGPIYALSAEVVAGLAVARNDSFRMLS 505
Query: 439 I*DVTIGSWMLAMNVNHENTHALCEPDCTESSVAVWDIPKCSGLCHPEVKMLELHQRKEC 260
DVTIG+WMLAMNV HE+ LC P C S+AVWDIPKCSGLC+P K+ ELH+ C
Sbjct: 504 NEDVTIGAWMLAMNVEHEDNRELCHPHCKPDSIAVWDIPKCSGLCNPAEKLPELHKNNMC 325
Query: 259 TGGPTVAAE 233
+ PT+ A+
Sbjct: 324 STSPTLPAD 298
Score = 130 bits (278), Expect = 1e-029
Identities = 63/130 (48%), Positives = 74/130 (56%)
Frame = +3 / +2
Query: 231 TSAATVGPPVHSFLXXXXXIFTSGWHSPEHFGMSQTATEDSVQSGSQSACVFSWLTFIAS 410
+SA +VG H F++G PEH G+S TA E +Q G ++ + S TFIAS
Sbjct: 296 SSAGSVGLVEHILFLCNSGSFSAGLQRPEHLGISHTAIESGLQWG*HNSRLSSCSTFIAS 475
Query: 411 IHDPIVTS*ILNIRXXXXXXXXXXXXXXXXXXXIGPYA*RKYSDPSKKDWGSYHFNLGSV 590
IH P+VTS +LNI IGPYA* KYS PSK GSYHFNLGSV
Sbjct: 476 IHAPMVTSSLLNILKLSFLATANPATTSAESAYIGPYA**KYSLPSKYAAGSYHFNLGSV 655
Query: 591 KTGPFFMHPM 620
TGPFFMHPM
Sbjct: 656 ITGPFFMHPM 685
>gb|BQ696389.1|BQ696389 NXPV_040_F05_F NXPV (Nsf Xylem Planings wood Vertical) Pinus taeda
cDNA clone NXPV_040_F05 5' similar to Arabidopsis
thaliana sequence At3g14960 galactosyltransferase,
putative see http://mips.gsf.de/proj/thal/db/index.html,
mRNA sequence
Length = 456
Score = 180 bits (388), Expect = 7e-045
Identities = 70/113 (61%), Positives = 83/113 (73%)
Frame = -2 / +1
Query: 598 PVFTDPKLKWYEPQSFLLGSEYFLHAYGPIYXXXXXXXXXXXXLRNNSFRMFSI*DVTIG 419
PVF DPKLKWYEP LLGSEYF+HAYGPIY LRN+S+RMFS DVTIG
Sbjct: 1 PVFRDPKLKWYEPLGDLLGSEYFIHAYGPIYALSAEIVAGLSGLRNDSYRMFSNEDVTIG 180
Query: 418 SWMLAMNVNHENTHALCEPDCTESSVAVWDIPKCSGLCHPEVKMLELHQRKEC 260
+WMLAMNVNHE+ LC P+CT +S+AVWDIPKCS L +P ++ ELH++ C
Sbjct: 181 AWMLAMNVNHEDNRELCSPECTPTSIAVWDIPKCSXLXNPAQRLXELHKKYVC 339
>gb|DR693981.1|DR693981 EST1084071 Normalized pine embryo library, Lib_D Pinus taeda cDNA
clone PWAE664 3' end, mRNA sequence
Length = 817
Score = 177 bits (381), Expect = 6e-044
Identities = 68/102 (66%), Positives = 74/102 (72%)
Frame = -2 / +3
Query: 619 IGCMKKGPVFTDPKLKWYEPQSFLLGSEYFLHAYGPIYXXXXXXXXXXXXLRNNSFRMFS 440
IGCMKKGPV TDPKLKWYEP ++LLGSEYF HAYGPIY RN+SFRM S
Sbjct: 444 IGCMKKGPVITDPKLKWYEPAAYLLGSEYFYHAYGPIYALSAEVVAGLAVARNDSFRMLS 623
Query: 439 I*DVTIGSWMLAMNVNHENTHALCEPDCTESSVAVWDIPKCS 314
DVTIG+WMLAMNV HE+ LC P C S+AVWDIPKCS
Sbjct: 624 NEDVTIGAWMLAMNVEHEDNRELCHPHCKPDSIAVWDIPKCS 749
Score = 116 bits (248), Expect = 1e-025
Identities = 55/102 (53%), Positives = 62/102 (60%)
Frame = +3 / -1
Query: 315 EHFGMSQTATEDSVQSGSQSACVFSWLTFIASIHDPIVTS*ILNIRXXXXXXXXXXXXXX 494
EH G+S TA E +Q G ++ + S TFIASIH P+VTS +LNI
Sbjct: 748 EHLGISHTAIESGLQWG*HNSRLSSCSTFIASIHAPMVTSSLLNILKLSFLATANPATTS 569
Query: 495 XXXXXIGPYA*RKYSDPSKKDWGSYHFNLGSVKTGPFFMHPM 620
IGPYA* KYS PSK GSYHFNLGSV TGPFFMHPM
Sbjct: 568 AESAYIGPYA**KYSLPSKYAAGSYHFNLGSVITGPFFMHPM 443
>gb|BI397792.1|BI397792 NXPV_107_E10_F NXPV (Nsf Xylem Planings wood Vertical) Pinus taeda
cDNA clone NXPV_107_E10 5' similar to Arabidopsis
thaliana sequence At3g14960 galactosyltransferase,
putative see http://mips.gsf.de/proj/thal/db/index.html,
mRNA sequence
Length = 267
Score = 127 bits (271), Expect = 9e-029
Identities = 52/86 (60%), Positives = 58/86 (67%)
Frame = -2 / +2
Query: 598 PVFTDPKLKWYEPQSFLLGSEYFLHAYGPIYXXXXXXXXXXXXLRNNSFRMFSI*DVTIG 419
PVF DPKLKWYEP LLGSEYF+HAYGPIY LRN+S+RMFS DVTIG
Sbjct: 8 PVFRDPKLKWYEPLGDLLGSEYFIHAYGPIYALSAEIVAGLSGLRNDSYRMFSNEDVTIG 187
Query: 418 SWMLAMNVNHENTHALCEPDCTESSV 341
+WMLAMNVNHE+ LC S+
Sbjct: 188 AWMLAMNVNHEDNRELCSXXXXXXSI 265
>gb|CX715693.1|CX715693 RTPQ1_35_H08.g1_A032 Roots treated with paraquat Pinus taeda cDNA
clone RTPQ1_35_H08_A032 5', mRNA sequence
Length = 649
Score = 123 bits (264), Expect = 9e-028
Identities = 47/78 (60%), Positives = 57/78 (73%)
Frame = -2 / -3
Query: 466 RNNSFRMFSI*DVTIGSWMLAMNVNHENTHALCEPDCTESSVAVWDIPKCSGLCHPEVKM 287
RN+SFRM S DVTIG+WMLAMNV HE+ LC P C S+AVWDIPKCSGLC+P K+
Sbjct: 626 RNDSFRMLSNEDVTIGAWMLAMNVEHEDNRELCHPHCKPDSIAVWDIPKCSGLCNPAEKL 447
Query: 286 LELHQRKECTGGPTVAAE 233
ELH+ C+ PT+ A+
Sbjct: 446 PELHKNNMCSTSPTLPAD 393
Database: Pinus_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:45 PM
Number of letters in database: 217,277,237
Number of sequences in database: 355,925
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 361,470,445
Number of Sequences: 355925
Number of extensions: 5270541
Number of successful extensions: 46868
Number of sequences better than 1.0e-020: 9
length of database: 72,425,745
effective HSP length: 51
effective length of database: 54,273,570
effective search space used: 8412403350
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)