TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3336981.2.1
(510 letters)
Database: Pinus_nucl_with_EST.fasta
355,925 sequences; 217,277,237 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|DT633478.1|DT633478 EST1148409 Normalized pine embryo li... 163 5e-048
gb|DR686433.1|DR686433 EST1076511 Normalized pine embryo li... 163 5e-048
gb|DR694004.1|DR694004 EST1084094 Normalized pine embryo li... 163 5e-048
gb|CF672023.1|CF672023 RTCNT1_60_G07.g1_A029 Root control P... 152 4e-045
>gb|DT633478.1|DT633478 EST1148409 Normalized pine embryo library, Lib_D Pinus taeda cDNA
clone PIMFV33 3' end, mRNA sequence
Length = 848
Score = 163 bits (351), Expect(2) = 5e-048
Identities = 68/141 (48%), Positives = 91/141 (64%)
Frame = +1 / +1
Query: 88 YSSIQSRCEVIMYTIALLTFMSIGGFPSFVEDVKVFRKERLSGHYGVSEFVISNTISATP 267
Y++I +R Y +TFMSIGGFPSFVED+KVF++ERL+GHYGV FVI+NT+S+ P
Sbjct: 307 YNAILARGSCASYVSGFMTFMSIGGFPSFVEDMKVFQRERLNGHYGVLAFVIANTLSSMP 486
Query: 268 YLAGVSLLPGAMLYYLTGLSKGADHFVYFXXXXXXXXXXXXXXXXXIAAVVPDFLMGIIV 447
YLA V+ + G + YY+ L G H++YF +A +VP+FLMGII
Sbjct: 487 YLALVATISGTITYYMVKLHPGFTHYLYFVLNLFSCMAVVESLMMTVATLVPNFLMGIIT 666
Query: 448 GAGVQGVMMLNGGFFRLPNEL 510
GAG+ G+ ML GFFR +L
Sbjct: 667 GAGILGIYMLVAGFFRFLKDL 729
Score = 47.3 bits (97), Expect(2) = 5e-048
Identities = 14/26 (53%), Positives = 21/26 (80%)
Frame = +1 / +1
Query: 1 VGYYWMRLAIYMGIGVCLGTIFYQLG 78
VGYYW+R+ IY + +C+GTI+Y +G
Sbjct: 223 VGYYWLRVVIYTFVTICIGTIYYNVG 300
>gb|DR686433.1|DR686433 EST1076511 Normalized pine embryo library, Lib_D Pinus taeda cDNA
clone PWABG02 3' end, mRNA sequence
Length = 798
Score = 163 bits (351), Expect(2) = 5e-048
Identities = 68/141 (48%), Positives = 91/141 (64%)
Frame = +1 / +2
Query: 88 YSSIQSRCEVIMYTIALLTFMSIGGFPSFVEDVKVFRKERLSGHYGVSEFVISNTISATP 267
Y++I +R Y +TFMSIGGFPSFVED+KVF++ERL+GHYGV FVI+NT+S+ P
Sbjct: 299 YNAILARGSCASYVSGFMTFMSIGGFPSFVEDMKVFQRERLNGHYGVLAFVIANTLSSMP 478
Query: 268 YLAGVSLLPGAMLYYLTGLSKGADHFVYFXXXXXXXXXXXXXXXXXIAAVVPDFLMGIIV 447
YLA V+ + G + YY+ L G H++YF +A +VP+FLMGII
Sbjct: 479 YLALVATISGTITYYMVKLHPGFTHYLYFVLNLFSCMAVVESLMMTVATLVPNFLMGIIT 658
Query: 448 GAGVQGVMMLNGGFFRLPNEL 510
GAG+ G+ ML GFFR +L
Sbjct: 659 GAGILGIYMLVAGFFRFLKDL 721
Score = 47.3 bits (97), Expect(2) = 5e-048
Identities = 14/26 (53%), Positives = 21/26 (80%)
Frame = +1 / +2
Query: 1 VGYYWMRLAIYMGIGVCLGTIFYQLG 78
VGYYW+R+ IY + +C+GTI+Y +G
Sbjct: 215 VGYYWLRVVIYTFVTICIGTIYYNVG 292
>gb|DR694004.1|DR694004 EST1084094 Normalized pine embryo library, Lib_D Pinus taeda cDNA
clone PWAE690 3' end, mRNA sequence
Length = 760
Score = 163 bits (351), Expect(2) = 5e-048
Identities = 68/141 (48%), Positives = 91/141 (64%)
Frame = +1 / +3
Query: 88 YSSIQSRCEVIMYTIALLTFMSIGGFPSFVEDVKVFRKERLSGHYGVSEFVISNTISATP 267
Y++I +R Y +TFMSIGGFPSFVED+KVF++ERL+GHYGV FVI+NT+S+ P
Sbjct: 195 YNAILARGSCASYVSGFMTFMSIGGFPSFVEDMKVFQRERLNGHYGVLAFVIANTLSSMP 374
Query: 268 YLAGVSLLPGAMLYYLTGLSKGADHFVYFXXXXXXXXXXXXXXXXXIAAVVPDFLMGIIV 447
YLA V+ + G + YY+ L G H++YF +A +VP+FLMGII
Sbjct: 375 YLALVATISGTITYYMVKLHPGFTHYLYFVLNLFSCMAVVESLMMTVATLVPNFLMGIIT 554
Query: 448 GAGVQGVMMLNGGFFRLPNEL 510
GAG+ G+ ML GFFR +L
Sbjct: 555 GAGILGIYMLVAGFFRFLKDL 617
Score = 47.3 bits (97), Expect(2) = 5e-048
Identities = 14/26 (53%), Positives = 21/26 (80%)
Frame = +1 / +3
Query: 1 VGYYWMRLAIYMGIGVCLGTIFYQLG 78
VGYYW+R+ IY + +C+GTI+Y +G
Sbjct: 111 VGYYWLRVVIYTFVTICIGTIYYNVG 188
>gb|CF672023.1|CF672023 RTCNT1_60_G07.g1_A029 Root control Pinus taeda cDNA clone
RTCNT1_60_G07_A029 5', mRNA sequence
Length = 768
Score = 152 bits (327), Expect(2) = 4e-045
Identities = 60/126 (47%), Positives = 83/126 (65%)
Frame = +1 / +2
Query: 82 STYSSIQSRCEVIMYTIALLTFMSIGGFPSFVEDVKVFRKERLSGHYGVSEFVISNTISA 261
++Y SI +R + +TFMSIGGFPSFVED+KVF++ERL+GHYGV FV+ NT+S
Sbjct: 389 TSYESILARGACASFVFGFVTFMSIGGFPSFVEDMKVFQRERLNGHYGVVAFVVGNTVSG 568
Query: 262 TPYLAGVSLLPGAMLYYLTGLSKGADHFVYFXXXXXXXXXXXXXXXXXIAAVVPDFLMGI 441
PYL +S++ G + YY+ GL G H+++F IA+VVP+FLMGI
Sbjct: 569 MPYLLLISVISGTICYYMVGLHPGIVHYIFFTAGLYASVTVVESLMMAIASVVPNFLMGI 748
Query: 442 IVGAGV 459
I+GAG+
Sbjct: 749 IIGAGI 766
Score = 48.7 bits (100), Expect(2) = 4e-045
Identities = 15/28 (53%), Positives = 24/28 (85%)
Frame = +1 / +2
Query: 1 VGYYWMRLAIYMGIGVCLGTIFYQLGYS 84
VGYYW+RL IY+ + +C+GTI++++G S
Sbjct: 311 VGYYWLRLVIYILLTICIGTIYFRIGTS 394
Database: Pinus_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:45 PM
Number of letters in database: 217,277,237
Number of sequences in database: 355,925
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 211,236,352
Number of Sequences: 355925
Number of extensions: 2259470
Number of successful extensions: 40727
Number of sequences better than 1.0e-020: 4
length of database: 72,425,745
effective HSP length: 51
effective length of database: 54,273,570
effective search space used: 6404281260
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)