TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3276201.2.1
(1827 letters)
Database: Pinus_nucl_with_EST.fasta
355,925 sequences; 217,277,237 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|DR110436.1|DR110436 RTS1_10_H02.g1_A029 Roots minus sulf... 154 3e-050
gb|DR014476.1|DR014476 HEAT1_49_F01.g1_A029 Root at 37 C fo... 155 8e-044
>gb|DR110436.1|DR110436 RTS1_10_H02.g1_A029 Roots minus sulfur Pinus taeda cDNA clone
RTS1_10_H02_A029 5', mRNA sequence
Length = 866
Score = 154 bits (331), Expect(3) = 3e-050
Identities = 64/162 (39%), Positives = 98/162 (60%)
Frame = +2 / +2
Query: 464 FPFGNAITKEILGLPAYEKWFTSRFSVATFENEMKWYSTEWTQNHEDYRVPDAMMNLMRK 643
FP G+ I + L Y +F F+ A F+NE+KWY TE + + +YR D ++ +
Sbjct: 44 FPLGSCINRSSLDNEDYVAFFVKNFNWAVFQNELKWYWTESQKGNYNYRDSDELLQFCKC 223
Query: 644 YKIKVRGHNVFWDDQNSQMQWVKPLNLAQLKAAMQKRLKSVVSPYAGKVIHWDVVNENLH 823
+ I+ RGH +FW+D N+ QWVK LN L+AA+QKR+ ++S Y GK H+DV NE LH
Sbjct: 224 HGIQTRGHCIFWEDINTVQQWVKELNQNDLRAAIQKRISDLLSRYRGKFQHYDVNNEMLH 403
Query: 824 FNFFETKLGPMASAQIYQQVGQLDRNAILFMNEFNTLEQPGD 949
+F++ +LG A +++ QLD A LF+N++N + D
Sbjct: 404 GSFYQDRLGKDIWAYMFKMAHQLDPFATLFVNDYNVEDGSDD 529
Score = 44.1 bits (90), Expect(3) = 3e-050
Identities = 19/60 (31%), Positives = 34/60 (56%)
Frame = +2 / +2
Query: 983 MNQIRGYAGNGGLKLGVGLESHFSTPNIPYMRSSLDTLAKLKLPMWLTEVDVVKSPNQVK 1162
+NQI G G+G++ H + P + ++L+T++ L LP+W TE+DV + V+
Sbjct: 551 VNQIVDLQERGAPVGGIGIQGHITYPAGSIVHTALNTMSILGLPIWFTEMDVYSTNEFVR 730
Score = 40.5 bits (82), Expect(3) = 3e-050
Identities = 14/23 (60%), Positives = 17/23 (73%)
Frame = +2 / +2
Query: 1166 LEQVLREGFAHPNVDGIVMWAAW 1234
LE +LRE FAHP V+GI+ W W
Sbjct: 740 LEVMLREAFAHPAVEGIMFWGFW 808
>gb|DR014476.1|DR014476 HEAT1_49_F01.g1_A029 Root at 37 C for 24 hr Pinus taeda cDNA clone
HEAT1_49_F01_A029 5', mRNA sequence
Length = 906
Score = 155 bits (332), Expect(2) = 8e-044
Identities = 65/172 (37%), Positives = 102/172 (59%)
Frame = +2 / +3
Query: 434 NVSIELLRLGFPFGNAITKEILGLPAYEKWFTSRFSVATFENEMKWYSTEWTQNHEDYRV 613
+V ++ FP G+ I + L Y +F F+ A F+NE+KWY TE + + +YR
Sbjct: 186 SVKVKQTYNSFPLGSCINRSSLDNEDYVAFFVKNFNWAVFQNELKWYWTESQKGNYNYRD 365
Query: 614 PDAMMNLMRKYKIKVRGHNVFWDDQNSQMQWVKPLNLAQLKAAMQKRLKSVVSPYAGKVI 793
D ++ + + I+ RGH +FW+D N+ QWVK LN L+AA+QKR+ ++S Y GK
Sbjct: 366 SDELLQFCKCHGIQTRGHCIFWEDINTVQQWVKELNQNDLRAAIQKRISDLLSRYRGKFQ 545
Query: 794 HWDVVNENLHFNFFETKLGPMASAQIYQQVGQLDRNAILFMNEFNTLEQPGD 949
H+DV NE LH +F++ +LG A +++ QLD A LF+N++N + D
Sbjct: 546 HYDVNNEMLHGSFYQDRLGKDIWAYMFKMAHQLDPFATLFVNDYNVEDGSDD 701
Score = 44.1 bits (90), Expect(2) = 8e-044
Identities = 19/60 (31%), Positives = 34/60 (56%)
Frame = +2 / +3
Query: 983 MNQIRGYAGNGGLKLGVGLESHFSTPNIPYMRSSLDTLAKLKLPMWLTEVDVVKSPNQVK 1162
+NQI G G+G++ H + P + ++L+T++ L LP+W TE+DV + V+
Sbjct: 723 VNQIVDLQERGAPVGGIGIQGHITYPAGSIVHTALNTMSILGLPIWFTEMDVYSTNEFVR 902
Database: Pinus_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:45 PM
Number of letters in database: 217,277,237
Number of sequences in database: 355,925
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 950,431,402
Number of Sequences: 355925
Number of extensions: 13912516
Number of successful extensions: 79113
Number of sequences better than 1.0e-020: 2
length of database: 72,425,745
effective HSP length: 55
effective length of database: 52,849,870
effective search space used: 29225978110
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)