TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3204272.2.1
(739 letters)
Database: Pinus_nucl_with_EST.fasta
355,925 sequences; 217,277,237 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CO366343.1|CO366343 RTK1_27_G06.b1_A029 Roots minus pota... 88 3e-033
gb|CO363686.1|CO363686 RTK1_11_F01.b1_A029 Roots minus pota... 88 3e-033
gb|DR090575.1|DR090575 RTAL1_16_B07.b1_A029 Roots plus adde... 67 3e-021
gb|DR090649.1|DR090649 RTAL1_16_B07.g1_A029 Roots plus adde... 67 3e-021
>gb|CO366343.1|CO366343 RTK1_27_G06.b1_A029 Roots minus potassium Pinus taeda cDNA clone
RTK1_27_G06_A029 3', mRNA sequence
Length = 884
Score = 87.7 bits (185), Expect(3) = 3e-033
Identities = 33/63 (52%), Positives = 39/63 (61%)
Frame = -3 / +2
Query: 665 LEKNRWNPDAIVFNSWQHYGTPSYWMQTLFRESSGAMFHPTTIXXXXXXXXXXXAITWQD 486
+ RWNPDAIVF+SWQ YGTPSYW+Q FRES+GA+ P TI AI W
Sbjct: 53 VNNRRWNPDAIVFDSWQQYGTPSYWVQQFFRESNGAVLLPVTIQENATSALVASAIRWSS 232
Query: 485 SEN 477
E+
Sbjct: 233 LED 241
Score = 58.8 bits (122), Expect(3) = 3e-033
Identities = 24/59 (40%), Positives = 37/59 (62%)
Frame = -3 / +2
Query: 398 SINALGSTATVLTSADVMDENSFSNPTKVVPVKSDLSNAAEQMQVTLAPRSFSSFDLAL 222
SI + GS T+LTS ++MDENSF P K+ P +++ N+ + ++L P S + DL L
Sbjct: 329 SIRSSGSKKTILTSDNLMDENSFDMPQKIAPKATEIVNSGTDINISLPPHSVTVVDLQL 505
Score = 33.6 bits (67), Expect(3) = 3e-033
Identities = 12/23 (52%), Positives = 18/23 (78%)
Frame = -3 / +2
Query: 473 FLRVKVINFGSDAVTLTISTSGL 405
++R+K +NFGS +TL I+ SGL
Sbjct: 251 YIRLKAVNFGSSLLTLDITISGL 319
>gb|CO363686.1|CO363686 RTK1_11_F01.b1_A029 Roots minus potassium Pinus taeda cDNA clone
RTK1_11_F01_A029 3', mRNA sequence
Length = 836
Score = 87.7 bits (185), Expect(3) = 3e-033
Identities = 33/63 (52%), Positives = 39/63 (61%)
Frame = -3 / +2
Query: 665 LEKNRWNPDAIVFNSWQHYGTPSYWMQTLFRESSGAMFHPTTIXXXXXXXXXXXAITWQD 486
+ RWNPDAIVF+SWQ YGTPSYW+Q FRES+GA+ P TI AI W
Sbjct: 5 VNNRRWNPDAIVFDSWQQYGTPSYWVQQFFRESNGAVLLPVTIQENATSALVASAIRWSS 184
Query: 485 SEN 477
E+
Sbjct: 185 LED 193
Score = 58.8 bits (122), Expect(3) = 3e-033
Identities = 24/59 (40%), Positives = 37/59 (62%)
Frame = -3 / +2
Query: 398 SINALGSTATVLTSADVMDENSFSNPTKVVPVKSDLSNAAEQMQVTLAPRSFSSFDLAL 222
SI + GS T+LTS ++MDENSF P K+ P +++ N+ + ++L P S + DL L
Sbjct: 281 SIRSSGSKKTILTSDNLMDENSFDMPQKIAPKATEIVNSGTDINISLPPHSVTVVDLQL 457
Score = 33.6 bits (67), Expect(3) = 3e-033
Identities = 12/23 (52%), Positives = 18/23 (78%)
Frame = -3 / +2
Query: 473 FLRVKVINFGSDAVTLTISTSGL 405
++R+K +NFGS +TL I+ SGL
Sbjct: 203 YIRLKAVNFGSSLLTLDITISGL 271
>gb|DR090575.1|DR090575 RTAL1_16_B07.b1_A029 Roots plus added aluminum Pinus taeda cDNA
clone RTAL1_16_B07_A029 3', mRNA sequence
Length = 778
Score = 67.0 bits (140), Expect(4) = 3e-021
Identities = 31/58 (53%), Positives = 36/58 (62%)
Frame = +1 / -3
Query: 481 SESCQVIADAASEPE*LELIVVGWNIAPLDSRKSVCIQ*LGVP*CCQELKTIASGFHL 654
S+ +IA+A IV G + APLDS K+ C Q*LGVP CC E TIASGFHL
Sbjct: 299 SKLLHLIAEATKADVAFSCIVTGRSTAPLDSLKNCCTQ*LGVPYCCHESNTIASGFHL 126
Score = 35.4 bits (71), Expect(4) = 3e-021
Identities = 21/66 (31%), Positives = 30/66 (45%)
Frame = +1 / -3
Query: 202 ATSLD*ARARSNELKERGASVTCICSAALLRSLFTGTTLVGLLKEFSSITSADVRTVAVD 381
A + *+ +S + E G I S+ G G+ KEFSSI +DVR V +
Sbjct: 587 AECIT*SSCKSTTVTECGGREMLISVPLFTISVALGAIFCGMSKEFSSIKLSDVRMVFFE 408
Query: 382 PRALIL 399
P +L
Sbjct: 407 PEERML 390
Score = 31.8 bits (63), Expect(4) = 3e-021
Identities = 13/23 (56%), Positives = 16/23 (69%)
Frame = +1 / -3
Query: 652 LFFSSPVRKAASANEARRLPLPA 720
LF S+P+RK AS A +LP PA
Sbjct: 71 LFVSNPIRKPASPIAANKLPCPA 3
Score = 22.1 bits (42), Expect(4) = 3e-021
Identities = 9/20 (45%), Positives = 12/20 (60%)
Frame = +1 / -3
Query: 409 PLVEIVRVTASDPKFMTFTL 468
PL+ + V+ PKF FTL
Sbjct: 377 PLIVMSSVSRELPKFTAFTL 318
>gb|DR090649.1|DR090649 RTAL1_16_B07.g1_A029 Roots plus added aluminum Pinus taeda cDNA
clone RTAL1_16_B07_A029 5', mRNA sequence
Length = 746
Score = 67.0 bits (140), Expect(4) = 3e-021
Identities = 31/58 (53%), Positives = 36/58 (62%)
Frame = +1 / -2
Query: 481 SESCQVIADAASEPE*LELIVVGWNIAPLDSRKSVCIQ*LGVP*CCQELKTIASGFHL 654
S+ +IA+A IV G + APLDS K+ C Q*LGVP CC E TIASGFHL
Sbjct: 298 SKLLHLIAEATKADVAFSCIVTGRSTAPLDSLKNCCTQ*LGVPYCCHESNTIASGFHL 125
Score = 35.4 bits (71), Expect(4) = 3e-021
Identities = 21/66 (31%), Positives = 30/66 (45%)
Frame = +1 / -2
Query: 202 ATSLD*ARARSNELKERGASVTCICSAALLRSLFTGTTLVGLLKEFSSITSADVRTVAVD 381
A + *+ +S + E G I S+ G G+ KEFSSI +DVR V +
Sbjct: 586 AECIT*SSCKSTTVTECGGREMLISVPLFTISVALGAIFCGMSKEFSSIKLSDVRMVFFE 407
Query: 382 PRALIL 399
P +L
Sbjct: 406 PEERML 389
Score = 31.8 bits (63), Expect(4) = 3e-021
Identities = 13/23 (56%), Positives = 16/23 (69%)
Frame = +1 / -2
Query: 652 LFFSSPVRKAASANEARRLPLPA 720
LF S+P+RK AS A +LP PA
Sbjct: 70 LFVSNPIRKPASPIAANKLPCPA 2
Score = 22.1 bits (42), Expect(4) = 3e-021
Identities = 9/20 (45%), Positives = 12/20 (60%)
Frame = +1 / -2
Query: 409 PLVEIVRVTASDPKFMTFTL 468
PL+ + V+ PKF FTL
Sbjct: 376 PLIVMSSVSRELPKFTAFTL 317
Database: Pinus_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:45 PM
Number of letters in database: 217,277,237
Number of sequences in database: 355,925
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 415,444,090
Number of Sequences: 355925
Number of extensions: 6423723
Number of successful extensions: 42870
Number of sequences better than 1.0e-020: 4
length of database: 72,425,745
effective HSP length: 52
effective length of database: 53,917,645
effective search space used: 10406105485
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)