TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3115474.2.1
(798 letters)
Database: Pinus_nucl_with_EST.fasta
355,925 sequences; 217,277,237 total letters
Score E
Sequences producing significant alignments: (bits) Value
dbj|BD266974.1| Compositions isolated from plant cells and ... 141 2e-057
gb|CF399989.1|CF399989 RTWW1_2_C12.g1_A015 Well-watered lob... 141 7e-056
gb|DR081108.1|DR081108 RTFEPL1_27_A10.g1_A029 Roots plus ad... 141 2e-054
gb|CO173085.1|CO173085 NDL1_33_H09.g1_A029 Needles control ... 140 3e-040
gb|CO198621.1|CO198621 GEO1_15_E06.b1_A029 Root gravitropis... 88 2e-034
gb|BF060537.1|BF060537 NXCI_116_D05_F NXCI (Nsf Xylem Compr... 95 1e-024
dbj|BD266975.1| Compositions isolated from plant cells and ... 88 9e-021
>dbj|BD266974.1| Compositions isolated from plant cells and utilization of the same in
modifying plant cell signal transduction
Length = 1489
Score = 141 bits (303), Expect(4) = 2e-057
Identities = 58/89 (65%), Positives = 70/89 (78%)
Frame = -3 / +2
Query: 445 HCRNGVVGNSTLGSLLHKKRIIVVDDNIVNLKVAAGALKKYGAEVTCADSGKEAITLLKP 266
H R +S L ++L K I+VVDDNIVN +VAAGALKKYGA V C DSGK AI++L+
Sbjct: 740 HLREPSKTSSPLSNVLSGKSILVVDDNIVNRRVAAGALKKYGANVICTDSGKSAISMLRQ 919
Query: 265 PHNFDACFMDIQMPEMDGFEATKRIRVME 179
PHNF+ACFMD+QMPEMDGFEAT++IR E
Sbjct: 920 PHNFNACFMDVQMPEMDGFEATRQIRAAE 1006
Score = 101 bits (215), Expect(2) = 8e-029
Identities = 50/81 (61%), Positives = 55/81 (67%)
Frame = +3 / -2
Query: 150 PRSICSFRSLSITLILFVASNPSISGICMSMKQASKL*GGFSSVIASFPLSAQVTSAPYF 329
P + S + S LI VASNPSISGIC SMK A KL G S IA FPLS Q+T APYF
Sbjct: 1035 PLLVHSMLASSAALICRVASNPSISGICTSMKHALKLCGCRSMDIADFPLSVQITLAPYF 856
Query: 330 FRAPAATLRFTILSSTTMIRF 392
F APAAT R T+LSSTT + F
Sbjct: 855 FSAPAATRRLTMLSSTTSMDF 793
Score = 79.4 bits (167), Expect(4) = 2e-057
Identities = 31/39 (79%), Positives = 34/39 (87%)
Frame = -3 / +2
Query: 118 QWRTPILAMTADVIQATHEQCLKSEMDGYVSKPFEGEQL 2
+W PILAMTADVIQATHE+CL+ MDGYVSKPFE EQL
Sbjct: 1061 RWHVPILAMTADVIQATHEECLRCGMDGYVSKPFEEEQL 1177
Score = 46.0 bits (94), Expect(2) = 8e-029
Identities = 21/38 (55%), Positives = 25/38 (65%)
Frame = +3 / -2
Query: 3 NCSPSNGLET*PSISDFKHCSCVAWITSAVMANIGVRH 116
+CS S G ET PS+ +H S VA ITSAV+A IG H
Sbjct: 1176 SCSSSKGFETYPSMPHRRHSSWVACITSAVIAKIGTCH 1063
Score = 35.9 bits (72), Expect(4) = 2e-057
Identities = 19/45 (42%), Positives = 26/45 (57%)
Frame = -3 / +2
Query: 595 KLFLLENPKSSVKSNSHIFREYNLNVIMKPLRASMLQVSLQRALG 461
K+ LL ++ ++ + VI+KPLRASML V LQ ALG
Sbjct: 587 KMILLATSLTAEETQKAKAAGFAETVILKPLRASMLAVCLQLALG 721
Score = 29.9 bits (59), Expect(4) = 2e-057
Identities = 12/27 (44%), Positives = 16/27 (59%)
Frame = -2 / +1
Query: 794 VTKYHLQRLGVQTELTTDLDQYISKNN 714
VTKYHLQR G++ + T +S N
Sbjct: 340 VTKYHLQRFGIEVDSITSSKVALSMLN 420
>gb|CF399989.1|CF399989 RTWW1_2_C12.g1_A015 Well-watered loblolly pine roots WW1 Pinus
taeda cDNA clone RTWW1_2_C12_A015 5', mRNA sequence
Length = 754
Score = 141 bits (303), Expect(3) = 7e-056
Identities = 58/89 (65%), Positives = 70/89 (78%)
Frame = -3 / +2
Query: 445 HCRNGVVGNSTLGSLLHKKRIIVVDDNIVNLKVAAGALKKYGAEVTCADSGKEAITLLKP 266
H R +S L ++L K I+VVDDNIVN +VAAGALKKYGA V C DSGK AI++L+
Sbjct: 152 HLREPSKTSSPLSNVLSGKSILVVDDNIVNRRVAAGALKKYGANVICTDSGKSAISMLRQ 331
Query: 265 PHNFDACFMDIQMPEMDGFEATKRIRVME 179
PHNF+ACFMD+QMPEMDGFEAT++IR E
Sbjct: 332 PHNFNACFMDVQMPEMDGFEATRQIRAAE 418
Score = 101 bits (215), Expect(2) = 8e-029
Identities = 50/81 (61%), Positives = 55/81 (67%)
Frame = +3 / -2
Query: 150 PRSICSFRSLSITLILFVASNPSISGICMSMKQASKL*GGFSSVIASFPLSAQVTSAPYF 329
P + S + S LI VASNPSISGIC SMK A KL G S IA FPLS Q+T APYF
Sbjct: 447 PLLVHSMLASSAALICRVASNPSISGICTSMKHALKLCGCRSMDIADFPLSVQITLAPYF 268
Query: 330 FRAPAATLRFTILSSTTMIRF 392
F APAAT R T+LSSTT + F
Sbjct: 267 FSAPAATRRLTMLSSTTSMDF 205
Score = 79.4 bits (167), Expect(3) = 7e-056
Identities = 31/39 (79%), Positives = 34/39 (87%)
Frame = -3 / +2
Query: 118 QWRTPILAMTADVIQATHEQCLKSEMDGYVSKPFEGEQL 2
+W PILAMTADVIQATHE+CL+ MDGYVSKPFE EQL
Sbjct: 473 RWHVPILAMTADVIQATHEECLRCGMDGYVSKPFEEEQL 589
Score = 46.0 bits (94), Expect(2) = 8e-029
Identities = 21/38 (55%), Positives = 25/38 (65%)
Frame = +3 / -2
Query: 3 NCSPSNGLET*PSISDFKHCSCVAWITSAVMANIGVRH 116
+CS S G ET PS+ +H S VA ITSAV+A IG H
Sbjct: 588 SCSSSKGFETYPSMPHRRHSSWVACITSAVIAKIGTCH 475
Score = 35.4 bits (71), Expect(3) = 7e-056
Identities = 16/20 (80%), Positives = 17/20 (85%)
Frame = -3 / +2
Query: 520 VIMKPLRASMLQVSLQRALG 461
VI+KPLRASML V LQ ALG
Sbjct: 74 VILKPLRASMLAVCLQLALG 133
>gb|DR081108.1|DR081108 RTFEPL1_27_A10.g1_A029 Roots plus added iron Pinus taeda cDNA clone
RTFEPL1_27_A10_A029 5', mRNA sequence
Length = 747
Score = 141 bits (303), Expect(3) = 2e-054
Identities = 58/89 (65%), Positives = 70/89 (78%)
Frame = -3 / +1
Query: 445 HCRNGVVGNSTLGSLLHKKRIIVVDDNIVNLKVAAGALKKYGAEVTCADSGKEAITLLKP 266
H R +S L ++L K I+VVDDNIVN +VAAGALKKYGA V C DSGK AI++L+
Sbjct: 319 HLREPSKTSSPLSNVLSGKSILVVDDNIVNRRVAAGALKKYGANVICTDSGKSAISMLRQ 498
Query: 265 PHNFDACFMDIQMPEMDGFEATKRIRVME 179
PHNF+ACFMD+QMPEMDGFEAT++IR E
Sbjct: 499 PHNFNACFMDVQMPEMDGFEATRQIRAAE 585
Score = 101 bits (215), Expect = 7e-021
Identities = 50/81 (61%), Positives = 55/81 (67%)
Frame = +3 / -2
Query: 150 PRSICSFRSLSITLILFVASNPSISGICMSMKQASKL*GGFSSVIASFPLSAQVTSAPYF 329
P + S + S LI VASNPSISGIC SMK A KL G S IA FPLS Q+T APYF
Sbjct: 614 PLLVHSMLASSAALICRVASNPSISGICTSMKHALKLCGCRSMDIADFPLSVQITLAPYF 435
Query: 330 FRAPAATLRFTILSSTTMIRF 392
F APAAT R T+LSSTT + F
Sbjct: 434 FSAPAATRRLTMLSSTTSMDF 372
Score = 73.9 bits (155), Expect(3) = 2e-054
Identities = 28/35 (80%), Positives = 31/35 (88%)
Frame = -3 / +1
Query: 118 QWRTPILAMTADVIQATHEQCLKSEMDGYVSKPFE 14
+W PILAMTADVIQATHE+CL+ MDGYVSKPFE
Sbjct: 640 RWHVPILAMTADVIQATHEECLRCGMDGYVSKPFE 744
Score = 35.9 bits (72), Expect(3) = 2e-054
Identities = 19/45 (42%), Positives = 26/45 (57%)
Frame = -3 / +1
Query: 595 KLFLLENPKSSVKSNSHIFREYNLNVIMKPLRASMLQVSLQRALG 461
K+ LL ++ ++ + VI+KPLRASML V LQ ALG
Sbjct: 166 KMILLATSLTAEETQKAKAAGFAETVILKPLRASMLAVCLQLALG 300
>gb|CO173085.1|CO173085 NDL1_33_H09.g1_A029 Needles control Pinus taeda cDNA clone
NDL1_33_H09_A029 5', mRNA sequence
Length = 771
Score = 140 bits (300), Expect(3) = 3e-040
Identities = 58/89 (65%), Positives = 69/89 (77%)
Frame = -3 / +3
Query: 445 HCRNGVVGNSTLGSLLHKKRIIVVDDNIVNLKVAAGALKKYGAEVTCADSGKEAITLLKP 266
H R +S L ++L K I+VVDDNIVN VAAGALKKYGA V C DSGK AI++L+
Sbjct: 426 HLREPSKTSSPLSNVLSGKSILVVDDNIVNRPVAAGALKKYGANVICTDSGKSAISMLRQ 605
Query: 265 PHNFDACFMDIQMPEMDGFEATKRIRVME 179
PHNF+ACFMD+QMPEMDGFEAT++IR E
Sbjct: 606 PHNFNACFMDVQMPEMDGFEATRQIRAAE 692
Score = 35.9 bits (72), Expect(3) = 3e-040
Identities = 19/45 (42%), Positives = 26/45 (57%)
Frame = -3 / +3
Query: 595 KLFLLENPKSSVKSNSHIFREYNLNVIMKPLRASMLQVSLQRALG 461
K+ LL ++ ++ + VI+KPLRASML V LQ ALG
Sbjct: 273 KMILLATSLTAEETQKAKAAGFAETVILKPLRASMLAVCLQLALG 407
Score = 31.3 bits (62), Expect(3) = 3e-040
Identities = 13/27 (48%), Positives = 16/27 (59%)
Frame = -2 / +3
Query: 794 VTKYHLQRLGVQTELTTDLDQYISKNN 714
VTKYHLQR G+Q + T +S N
Sbjct: 27 VTKYHLQRFGIQVDSITSSKVALSMLN 107
>gb|CO198621.1|CO198621 GEO1_15_E06.b1_A029 Root gravitropism April 2003 test Pinus taeda
cDNA clone GEO1_15_E06_A029 3', mRNA sequence
Length = 724
Score = 87.7 bits (185), Expect(2) = 2e-034
Identities = 33/50 (66%), Positives = 40/50 (80%)
Frame = -3 / -1
Query: 274 LKPPHNFDACFMDIQMPEMDGFEATKRIRVMERDLNEQIERGEAPPECVG 125
LKPPH FDACFMD+QMPEMDGFEAT+++R +ER +NEQI+ G E G
Sbjct: 724 LKPPHKFDACFMDVQMPEMDGFEATRQVRDLERTVNEQIDSGAISKEIDG 575
Score = 79.0 bits (166), Expect(2) = 2e-034
Identities = 31/41 (75%), Positives = 34/41 (82%)
Frame = -3 / -1
Query: 124 VRQWRTPILAMTADVIQATHEQCLKSEMDGYVSKPFEGEQL 2
+ +W PILAMTADVIQATHE+C K MDGYVSKPFE EQL
Sbjct: 571 ISKWHVPILAMTADVIQATHEECSKCGMDGYVSKPFEEEQL 449
Score = 65.7 bits (137), Expect(2) = 1e-021
Identities = 26/38 (68%), Positives = 30/38 (78%)
Frame = +3 / +3
Query: 3 NCSPSNGLET*PSISDFKHCSCVAWITSAVMANIGVRH 116
+CS SNG +T*PSI +H SCVAWITSAV+ANIG H
Sbjct: 450 SCSSSNGFDT*PSIPHLEHSSCVAWITSAVIANIGTCH 563
Score = 57.4 bits (119), Expect(2) = 1e-021
Identities = 28/48 (58%), Positives = 31/48 (64%)
Frame = +3 / +3
Query: 126 PTHSGGASPRSICSFRSLSITLILFVASNPSISGICMSMKQASKL*GG 269
P+ S +P SICS S + VASNPSISGIC SMK AS L*GG
Sbjct: 576 PSISFEIAPESICSLTVRSRSRTCLVASNPSISGICTSMKHASNL*GG 719
>gb|BF060537.1|BF060537 NXCI_116_D05_F NXCI (Nsf Xylem Compression wood Inclined) Pinus
taeda cDNA clone NXCI_116_D05 5' similar to Arabidopsis
thaliana sequence At2g01830 cytokinin receptor CRE1b
(CRE1) see http://mips.gsf.de/proj/thal/db/index.html,
mRNA sequence
Length = 459
Score = 95.0 bits (201), Expect(2) = 1e-024
Identities = 40/64 (62%), Positives = 47/64 (73%)
Frame = -3 / +3
Query: 424 GNSTLGSLLHKKRIIVVDDNIVNLKVAAGALKKYGAEVTCADSGKEAITLLKPPHNFDAC 245
G L SLL K+I+VVDDN VN +V ALKKYGA V CA+SGK AI +L+ PH FDAC
Sbjct: 267 GPPFLQSLLFGKKILVVDDNRVNRRVXXXALKKYGANVECAESGKNAIAMLQLPHKFDAC 446
Query: 244 FMDI 233
FMD+
Sbjct: 447 FMDV 458
Score = 38.6 bits (78), Expect(2) = 1e-024
Identities = 20/48 (41%), Positives = 28/48 (58%)
Frame = -3 / +3
Query: 604 DSTKLFLLENPKSSVKSNSHIFREYNLNVIMKPLRASMLQVSLQRALG 461
+S K+ LL +S++S + VIMKPLRASM+ LQ+ LG
Sbjct: 84 ESLKMILLATSITSLESEKAKAAGFVETVIMKPLRASMVAACLQQELG 227
>dbj|BD266975.1| Compositions isolated from plant cells and utilization of the same
in modifying plant cell signal transduction
Length = 370
Score = 88.1 bits (186), Expect(2) = 9e-021
Identities = 37/63 (58%), Positives = 46/63 (73%)
Frame = -3 / +2
Query: 445 HCRNGVVGNSTLGSLLHKKRIIVVDDNIVNLKVAAGALKKYGAEVTCADSGKEAITLLKP 266
H R + +S L ++L K I+VVDDNIVN +VAAGALKKYGA V C D GK AI++L+
Sbjct: 170 HLREPLKTSSPLSNVLSGKGILVVDDNIVNRRVAAGALKKYGANVICTDGGKSAISMLRQ 349
Query: 265 PHN 257
PHN
Sbjct: 350 PHN 358
Score = 32.2 bits (64), Expect(2) = 9e-021
Identities = 17/45 (37%), Positives = 25/45 (55%)
Frame = -3 / +2
Query: 595 KLFLLENPKSSVKSNSHIFREYNLNVIMKPLRASMLQVSLQRALG 461
K+ LL ++ ++ + VI+KPLRAS+ V LQ ALG
Sbjct: 17 KIILLATSLTAEETQKAKAAGFAETVILKPLRASVFAVRLQLALG 151
Database: Pinus_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:45 PM
Number of letters in database: 217,277,237
Number of sequences in database: 355,925
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 481,893,298
Number of Sequences: 355925
Number of extensions: 7521274
Number of successful extensions: 46878
Number of sequences better than 1.0e-020: 8
length of database: 72,425,745
effective HSP length: 52
effective length of database: 53,917,645
effective search space used: 11484458385
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)