TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3054736.2.1
(725 letters)
Database: Pinus_nucl_with_EST.fasta
355,925 sequences; 217,277,237 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|DR694977.1|DR694977 EST1085070 Normalized pine embryo li... 193 2e-048
gb|DT638989.1|DT638989 EST1153920 Normalized pine embryo li... 193 2e-048
gb|DR109699.1|DR109699 RTS1_4_C10.b1_A029 Roots minus sulfu... 190 1e-047
gb|BQ696389.1|BQ696389 NXPV_040_F05_F NXPV (Nsf Xylem Plani... 177 8e-044
gb|DR693981.1|DR693981 EST1084071 Normalized pine embryo li... 155 4e-037
gb|CX715693.1|CX715693 RTPQ1_35_H08.g1_A032 Roots treated w... 133 2e-030
gb|BI397792.1|BI397792 NXPV_107_E10_F NXPV (Nsf Xylem Plani... 127 1e-028
>gb|DR694977.1|DR694977 EST1085070 Normalized pine embryo library, Lib_D Pinus taeda cDNA
clone PWAEJ07 3' end, mRNA sequence
Length = 793
Score = 193 bits (415), Expect = 2e-048
Identities = 75/118 (63%), Positives = 89/118 (75%)
Frame = +3 / +1
Query: 3 VVNDPNLKWYESSWELLGNEYFMHASGSLYALSSEVVEALATAKSDSLRMFDYEDVTIGA 182
V DP LKWYE +LLG+EYF+HA G +YALS+E+V L+ ++DS RMF EDVTIGA
Sbjct: 244 VFRDPKLKWYEPLGDLLGSEYFIHAYGPIYALSAEIVAGLSGLRNDSYRMFSNEDVTIGA 423
Query: 183 WMLAMNVKHEDNRAMCDSVCTPTSIAVWDSKKCSGTCNVADKIKQLHNTTVCSKSPTL 356
WMLAMNV HEDNR +C CTPTSIAVWD KCSG CN A ++++LH VC KSPTL
Sbjct: 424 WMLAMNVNHEDNRELCSPECTPTSIAVWDIPKCSGLCNPAQRLRELHKKYVCMKSPTL 597
Score = 157 bits (337), Expect = 9e-038
Identities = 74/120 (61%), Positives = 84/120 (70%)
Frame = -2 / -3
Query: 361 GGNVGLFEHTVVLWSCFILSATLQVPEHFLLSHTAMEVGVHTESHIARLSSCFTFIASIH 182
GGNVGLF T LWS L A LQ PEHF +SHTA+EVGVH+ H +RLSS TFIASIH
Sbjct: 602 GGNVGLFIQTYFLWSSLSLCAGLQRPEHFGISHTAIEVGVHSGEHSSRLSSWLTFIASIH 423
Query: 181 APIVTSS*SNILKLSLFAVARASTTSEERAYSEPDACMKYSLPNSSQELSYHFKFGSLTT 2
AP+VTSS NIL LS + + +T S ERAY P A MKYSLP+ S + SYHF GSL T
Sbjct: 422 APMVTSSLLNIL*LSFLSPDKPATISAERAYIGP*A*MKYSLPSKSPKGSYHFNLGSLKT 243
>gb|DT638989.1|DT638989 EST1153920 Normalized pine embryo library, Lib_D Pinus taeda cDNA
clone PIMHL79 3' end, mRNA sequence
Length = 850
Score = 193 bits (415), Expect = 2e-048
Identities = 75/118 (63%), Positives = 89/118 (75%)
Frame = +3 / +3
Query: 3 VVNDPNLKWYESSWELLGNEYFMHASGSLYALSSEVVEALATAKSDSLRMFDYEDVTIGA 182
V DP LKWYE +LLG+EYF+HA G +YALS+E+V L+ ++DS RMF EDVTIGA
Sbjct: 252 VFRDPKLKWYEPLGDLLGSEYFIHAYGPIYALSAEIVAGLSGLRNDSYRMFSNEDVTIGA 431
Query: 183 WMLAMNVKHEDNRAMCDSVCTPTSIAVWDSKKCSGTCNVADKIKQLHNTTVCSKSPTL 356
WMLAMNV HEDNR +C CTPTSIAVWD KCSG CN A ++++LH VC KSPTL
Sbjct: 432 WMLAMNVNHEDNRELCSPECTPTSIAVWDIPKCSGLCNPAQRLRELHKKYVCMKSPTL 605
Score = 157 bits (337), Expect = 9e-038
Identities = 74/120 (61%), Positives = 84/120 (70%)
Frame = -2 / -1
Query: 361 GGNVGLFEHTVVLWSCFILSATLQVPEHFLLSHTAMEVGVHTESHIARLSSCFTFIASIH 182
GGNVGLF T LWS L A LQ PEHF +SHTA+EVGVH+ H +RLSS TFIASIH
Sbjct: 610 GGNVGLFIQTYFLWSSLSLCAGLQRPEHFGISHTAIEVGVHSGEHSSRLSSWLTFIASIH 431
Query: 181 APIVTSS*SNILKLSLFAVARASTTSEERAYSEPDACMKYSLPNSSQELSYHFKFGSLTT 2
AP+VTSS NIL LS + + +T S ERAY P A MKYSLP+ S + SYHF GSL T
Sbjct: 430 APMVTSSLLNIL*LSFLSPDKPATISAERAYIGP*A*MKYSLPSKSPKGSYHFNLGSLKT 251
>gb|DR109699.1|DR109699 RTS1_4_C10.b1_A029 Roots minus sulfur Pinus taeda cDNA clone
RTS1_4_C10_A029 3', mRNA sequence
Length = 808
Score = 190 bits (409), Expect = 1e-047
Identities = 75/118 (63%), Positives = 88/118 (74%)
Frame = +3 / -2
Query: 3 VVNDPNLKWYESSWELLGNEYFMHASGSLYALSSEVVEALATAKSDSLRMFDYEDVTIGA 182
V+ DP LKWYE + LLG+EYF HA G +YALS+EVV LA A++DS RM EDVTIGA
Sbjct: 660 VITDPKLKWYEPAAYLLGSEYFYHAYGPIYALSAEVVAGLAVARNDSFRMLSNEDVTIGA 481
Query: 183 WMLAMNVKHEDNRAMCDSVCTPTSIAVWDSKKCSGTCNVADKIKQLHNTTVCSKSPTL 356
WMLAMNV+HEDNR +C C P SIAVWD KCSG CN A+K+ +LH +CS SPTL
Sbjct: 480 WMLAMNVEHEDNRELCHPHCKPDSIAVWDIPKCSGLCNPAEKLPELHKNNMCSTSPTL 307
Score = 135 bits (290), Expect = 3e-031
Identities = 67/119 (56%), Positives = 78/119 (65%)
Frame = -2 / +2
Query: 358 GNVGLFEHTVVLWSCFILSATLQVPEHFLLSHTAMEVGVHTESHIARLSSCFTFIASIHA 179
G+VGL EH + L + SA LQ PEH +SHTA+E G+ H +RLSSC TFIASIHA
Sbjct: 305 GSVGLVEHILFLCNSGSFSAGLQRPEHLGISHTAIESGLQWG*HNSRLSSCSTFIASIHA 484
Query: 178 PIVTSS*SNILKLSLFAVARASTTSEERAYSEPDACMKYSLPNSSQELSYHFKFGSLTT 2
P+VTSS NILKLS A A +TTS E AY P A KYSLP+ SYHF GS+ T
Sbjct: 485 PMVTSSLLNILKLSFLATANPATTSAESAYIGPYA**KYSLPSKYAAGSYHFNLGSVIT 661
>gb|BQ696389.1|BQ696389 NXPV_040_F05_F NXPV (Nsf Xylem Planings wood Vertical) Pinus taeda
cDNA clone NXPV_040_F05 5' similar to Arabidopsis
thaliana sequence At3g14960 galactosyltransferase,
putative see http://mips.gsf.de/proj/thal/db/index.html,
mRNA sequence
Length = 456
Score = 177 bits (381), Expect = 8e-044
Identities = 71/118 (60%), Positives = 84/118 (71%)
Frame = +3 / +1
Query: 3 VVNDPNLKWYESSWELLGNEYFMHASGSLYALSSEVVEALATAKSDSLRMFDYEDVTIGA 182
V DP LKWYE +LLG+EYF+HA G +YALS+E+V L+ ++DS RMF EDVTIGA
Sbjct: 4 VFRDPKLKWYEPLGDLLGSEYFIHAYGPIYALSAEIVAGLSGLRNDSYRMFSNEDVTIGA 183
Query: 183 WMLAMNVKHEDNRAMCDSVCTPTSIAVWDSKKCSGTCNVADKIKQLHNTTVCSKSPTL 356
WMLAMNV HEDNR +C CTPTSIAVWD KCS N A ++ +LH VC K TL
Sbjct: 184 WMLAMNVNHEDNRELCSPECTPTSIAVWDIPKCSXLXNPAQRLXELHKKYVCMKXXTL 357
Score = 148 bits (317), Expect = 5e-035
Identities = 71/120 (59%), Positives = 81/120 (67%)
Frame = -2 / -2
Query: 361 GGNVGLFEHTVVLWSCFILSATLQVPEHFLLSHTAMEVGVHTESHIARLSSCFTFIASIH 182
GGNV LF T LWS L A Q EHF +SHTA+EVGVH+ H +RLSS TFIASIH
Sbjct: 362 GGNVXLFIQTYFLWSSXSLCAGXQXXEHFGISHTAIEVGVHSGEHSSRLSSWLTFIASIH 183
Query: 181 APIVTSS*SNILKLSLFAVARASTTSEERAYSEPDACMKYSLPNSSQELSYHFKFGSLTT 2
AP+VTSS NIL LS + + +T S ERAY P A MKYSLP+ S + SYHF GSL T
Sbjct: 182 APMVTSSLLNIL*LSFLSPDKPATISAERAYIGP*A*MKYSLPSKSPKGSYHFNLGSLKT 3
>gb|DR693981.1|DR693981 EST1084071 Normalized pine embryo library, Lib_D Pinus taeda cDNA
clone PWAE664 3' end, mRNA sequence
Length = 817
Score = 155 bits (332), Expect = 4e-037
Identities = 62/94 (65%), Positives = 71/94 (75%)
Frame = +3 / +3
Query: 3 VVNDPNLKWYESSWELLGNEYFMHASGSLYALSSEVVEALATAKSDSLRMFDYEDVTIGA 182
V+ DP LKWYE + LLG+EYF HA G +YALS+EVV LA A++DS RM EDVTIGA
Sbjct: 468 VITDPKLKWYEPAAYLLGSEYFYHAYGPIYALSAEVVAGLAVARNDSFRMLSNEDVTIGA 647
Query: 183 WMLAMNVKHEDNRAMCDSVCTPTSIAVWDSKKCS 284
WMLAMNV+HEDNR +C C P SIAVWD KCS
Sbjct: 648 WMLAMNVEHEDNRELCHPHCKPDSIAVWDIPKCS 749
Score = 113 bits (242), Expect = 1e-024
Identities = 55/94 (58%), Positives = 63/94 (67%)
Frame = -2 / -1
Query: 283 EHFLLSHTAMEVGVHTESHIARLSSCFTFIASIHAPIVTSS*SNILKLSLFAVARASTTS 104
EH +SHTA+E G+ H +RLSSC TFIASIHAP+VTSS NILKLS A A +TTS
Sbjct: 748 EHLGISHTAIESGLQWG*HNSRLSSCSTFIASIHAPMVTSSLLNILKLSFLATANPATTS 569
Query: 103 EERAYSEPDACMKYSLPNSSQELSYHFKFGSLTT 2
E AY P A KYSLP+ SYHF GS+ T
Sbjct: 568 AESAYIGPYA**KYSLPSKYAAGSYHFNLGSVIT 467
>gb|CX715693.1|CX715693 RTPQ1_35_H08.g1_A032 Roots treated with paraquat Pinus taeda cDNA
clone RTPQ1_35_H08_A032 5', mRNA sequence
Length = 649
Score = 133 bits (284), Expect = 2e-030
Identities = 52/82 (63%), Positives = 60/82 (73%)
Frame = +3 / -3
Query: 111 VEALATAKSDSLRMFDYEDVTIGAWMLAMNVKHEDNRAMCDSVCTPTSIAVWDSKKCSGT 290
V LA A++DS RM EDVTIGAWMLAMNV+HEDNR +C C P SIAVWD KCSG
Sbjct: 647 VAGLAVARNDSFRMLSNEDVTIGAWMLAMNVEHEDNRELCHPHCKPDSIAVWDIPKCSGL 468
Query: 291 CNVADKIKQLHNTTVCSKSPTL 356
CN A+K+ +LH +CS SPTL
Sbjct: 467 CNPAEKLPELHKNNMCSTSPTL 402
>gb|BI397792.1|BI397792 NXPV_107_E10_F NXPV (Nsf Xylem Planings wood Vertical) Pinus taeda
cDNA clone NXPV_107_E10 5' similar to Arabidopsis
thaliana sequence At3g14960 galactosyltransferase,
putative see http://mips.gsf.de/proj/thal/db/index.html,
mRNA sequence
Length = 267
Score = 127 bits (271), Expect = 1e-028
Identities = 51/85 (60%), Positives = 61/85 (71%)
Frame = +3 / +2
Query: 3 VVNDPNLKWYESSWELLGNEYFMHASGSLYALSSEVVEALATAKSDSLRMFDYEDVTIGA 182
V DP LKWYE +LLG+EYF+HA G +YALS+E+V L+ ++DS RMF EDVTIGA
Sbjct: 11 VFRDPKLKWYEPLGDLLGSEYFIHAYGPIYALSAEIVAGLSGLRNDSYRMFSNEDVTIGA 190
Query: 183 WMLAMNVKHEDNRAMCDSVCTPTSI 257
WMLAMNV HEDNR +C SI
Sbjct: 191 WMLAMNVNHEDNRELCSXXXXXXSI 265
Database: Pinus_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:45 PM
Number of letters in database: 217,277,237
Number of sequences in database: 355,925
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 426,060,397
Number of Sequences: 355925
Number of extensions: 6017265
Number of successful extensions: 43690
Number of sequences better than 1.0e-020: 9
length of database: 72,425,745
effective HSP length: 52
effective length of database: 53,917,645
effective search space used: 10190434905
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)