TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2750560.2.1
(660 letters)
Database: Pinus_nucl_with_EST.fasta
355,925 sequences; 217,277,237 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CF386596.1|CF386596 RTDR1_15_D11.g1_A015 Loblolly pine r... 120 3e-043
gb|DR013334.1|DR013334 HEAT1_18_E06.g1_A029 Root at 37 C fo... 64 3e-021
>gb|CF386596.1|CF386596 RTDR1_15_D11.g1_A015 Loblolly pine roots recovering from drought
DR1 Pinus taeda cDNA clone RTDR1_15_D11_A015 5', mRNA
sequence
Length = 763
Score = 120 bits (256), Expect(3) = 3e-043
Identities = 44/62 (70%), Positives = 55/62 (88%)
Frame = +3 / +1
Query: 270 LFRELWHACAGPLVTVPRQGELVYYFPQGHMEQLEASTDQQLDQHLPLFDLPPKILCKVV 449
L+ ELWHACAGPLVTVPR GE V+YFPQGHMEQ+EAST+Q DQ +PL++L P+ILC+V+
Sbjct: 349 LYEELWHACAGPLVTVPRVGERVFYFPQGHMEQVEASTNQGADQQMPLYNLMPRILCRVI 528
Query: 450 NV 455
N+
Sbjct: 529 NL 534
Score = 69.3 bits (145), Expect(3) = 3e-043
Identities = 27/29 (93%), Positives = 29/29 (100%)
Frame = +3 / +2
Query: 573 NVYSFCKTLTASDTSTHGGFSVLRRHAEE 659
NV+SFCKTLTASDTSTHGGFSVLRRHA+E
Sbjct: 659 NVHSFCKTLTASDTSTHGGFSVLRRHADE 745
Score = 24.4 bits (47), Expect(3) = 3e-043
Identities = 10/18 (55%), Positives = 16/18 (88%)
Frame = +2 / +1
Query: 485 SLCSNYAATRSRSK*AHQ 538
S+C++Y+A+RS +K*A Q
Sbjct: 568 SVCADYSASRS*AK*AVQ 621
>gb|DR013334.1|DR013334 HEAT1_18_E06.g1_A029 Root at 37 C for 24 hr Pinus taeda cDNA clone
HEAT1_18_E06_A029 5', mRNA sequence
Length = 663
Score = 63.8 bits (133), Expect(2) = 3e-021
Identities = 21/31 (67%), Positives = 27/31 (87%)
Frame = +3 / +2
Query: 279 ELWHACAGPLVTVPRQGELVYYFPQGHMEQL 371
ELWHACAGPL+++P +G V YFPQGH+EQ+
Sbjct: 317 ELWHACAGPLISLPPKGSRVVYFPQGHLEQI 409
Score = 57.9 bits (120), Expect(2) = 3e-021
Identities = 22/47 (46%), Positives = 34/47 (72%)
Frame = +3 / +2
Query: 375 ASTDQQLDQHLPLFDLPPKILCKVVNVELRAETDSDEVYAQIMLQPE 515
+S Q ++Q + + LPP+ILC+V+NV L A+ + DEVYAQ+ L P+
Sbjct: 521 SSISQAVNQQMLSYKLPPQILCRVLNVNLHADQEMDEVYAQLTLVPD 661
Database: Pinus_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:45 PM
Number of letters in database: 217,277,237
Number of sequences in database: 355,925
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 317,375,937
Number of Sequences: 355925
Number of extensions: 3926010
Number of successful extensions: 40573
Number of sequences better than 1.0e-020: 3
length of database: 72,425,745
effective HSP length: 52
effective length of database: 53,917,645
effective search space used: 9004246715
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)