TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2621786.2.1
(623 letters)
Database: Pinus_nucl_with_EST.fasta
355,925 sequences; 217,277,237 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CO161442.1|CO161442 FLD1_28_G04.g1_A029 Root flooded Pin... 237 7e-062
gb|CX649085.1|CX649085 COLD1_32_H04.g1_A029 Root cold Pinus... 237 7e-062
gb|CF389110.1|CF389110 RTDR2_13_F12.g1_A021 Loblolly pine r... 236 1e-061
gb|BX251594.1|BX251594 BX251594 Pinus pinaster differenciat... 234 4e-061
gb|DR049410.1|DR049410 RTBOR1_16_G01.b1_A029 Roots plus add... 213 1e-054
gb|DR688225.1|DR688225 EST1078309 Normalized pine embryo li... 199 1e-050
gb|BE187175.1|BE187175 NXNV_160_B08_F Nsf Xylem Normal wood... 176 1e-043
gb|CF389075.1|CF389075 RTDR2_13_F12.b1_A021 Loblolly pine r... 107 8e-023
>gb|CO161442.1|CO161442 FLD1_28_G04.g1_A029 Root flooded Pinus taeda cDNA clone
FLD1_28_G04_A029 5', mRNA sequence
Length = 879
Score = 237 bits (511), Expect = 7e-062
Identities = 91/124 (73%), Positives = 113/124 (91%)
Frame = +2 / -3
Query: 5 SARDWGFAGDYVEAMWLMLQQEQPGDYVVATEESHTVEEFLQAAFGYAGLSWKDHVVIDK 184
++RDWGFAGDYVEAMWLMLQQ++P DYVVAT++SHTVEEFL+ AFGY GL WK+HV+ID
Sbjct: 700 ASRDWGFAGDYVEAMWLMLQQDKPDDYVVATDDSHTVEEFLKEAFGYVGLDWKEHVLIDP 521
Query: 185 KYFRPAEVDSLKGDATKARKVLKWKPKVGFQELVEMMVDHDIELAKKEKVLVDAGFRDPK 364
+YFRPAEVD+L+GD++KAR++L W PKVGF++LV MMVD D+ELAK+EKVLVDAG+ D +
Sbjct: 520 RYFRPAEVDNLRGDSSKARRLLGWAPKVGFKQLVAMMVDSDLELAKREKVLVDAGYIDSQ 341
Query: 365 QQP* 376
QQP*
Sbjct: 340 QQP* 329
Score = 155 bits (333), Expect = 3e-037
Identities = 73/120 (60%), Positives = 81/120 (67%)
Frame = -3 / +3
Query: 372 GCCLGSRNPASTRTFSFLAXXXXXXXXXXXXSWNPTLGFHLSTFLALVASPLRLSTSAGR 193
GCC S PASTRTFS LA NPT G H + LAL SPLRLSTSAG
Sbjct: 333 GCC*ESMYPASTRTFSLLANSKSESTIMATNCLNPTFGAHPRSLLALDESPLRLSTSAGL 512
Query: 192 KYFLSMTT*SFQLRPA*PKAACRNSSTVWDSSVATT*SPGCSCCSMSHIAST*SPANPQS 13
KY S++T SFQ P P A+ +NSSTVW SSVATT*S G SCC+++HIAST*SPANPQS
Sbjct: 513 KYLGSISTCSFQSNPTYPNASFKNSSTVWLSSVATT*SSGLSCCNINHIAST*SPANPQS 692
>gb|CX649085.1|CX649085 COLD1_32_H04.g1_A029 Root cold Pinus taeda cDNA clone
COLD1_32_H04_A029 5', mRNA sequence
Length = 754
Score = 237 bits (511), Expect = 7e-062
Identities = 91/124 (73%), Positives = 113/124 (91%)
Frame = +2 / +1
Query: 5 SARDWGFAGDYVEAMWLMLQQEQPGDYVVATEESHTVEEFLQAAFGYAGLSWKDHVVIDK 184
++RDWGFAGDYVEAMWLMLQQ++P DYVVAT++SHTVEEFL+ AFGY GL WK+HV+ID
Sbjct: 310 ASRDWGFAGDYVEAMWLMLQQDKPDDYVVATDDSHTVEEFLKEAFGYVGLDWKEHVLIDP 489
Query: 185 KYFRPAEVDSLKGDATKARKVLKWKPKVGFQELVEMMVDHDIELAKKEKVLVDAGFRDPK 364
+YFRPAEVD+L+GD++KAR++L W PKVGF++LV MMVD D+ELAK+EKVLVDAG+ D +
Sbjct: 490 RYFRPAEVDNLRGDSSKARRLLGWAPKVGFKQLVAMMVDSDLELAKREKVLVDAGYIDSQ 669
Query: 365 QQP* 376
QQP*
Sbjct: 670 QQP* 681
Score = 155 bits (333), Expect = 3e-037
Identities = 73/120 (60%), Positives = 81/120 (67%)
Frame = -3 / -3
Query: 372 GCCLGSRNPASTRTFSFLAXXXXXXXXXXXXSWNPTLGFHLSTFLALVASPLRLSTSAGR 193
GCC S PASTRTFS LA NPT G H + LAL SPLRLSTSAG
Sbjct: 677 GCC*ESMYPASTRTFSLLANSKSESTIMATNCLNPTFGAHPRSLLALDESPLRLSTSAGL 498
Query: 192 KYFLSMTT*SFQLRPA*PKAACRNSSTVWDSSVATT*SPGCSCCSMSHIAST*SPANPQS 13
KY S++T SFQ P P A+ +NSSTVW SSVATT*S G SCC+++HIAST*SPANPQS
Sbjct: 497 KYLGSISTCSFQSNPTYPNASFKNSSTVWLSSVATT*SSGLSCCNINHIAST*SPANPQS 318
>gb|CF389110.1|CF389110 RTDR2_13_F12.g1_A021 Loblolly pine roots recovering from drought
DR2 Pinus taeda cDNA clone RTDR2_13_F12_A021 5', mRNA
sequence
Length = 632
Score = 236 bits (510), Expect = 1e-061
Identities = 91/123 (73%), Positives = 112/123 (91%)
Frame = +2 / -1
Query: 8 ARDWGFAGDYVEAMWLMLQQEQPGDYVVATEESHTVEEFLQAAFGYAGLSWKDHVVIDKK 187
+RDWGFAGDYVEAMWLMLQQ++P DYVVAT++SHTVEEFL+ AFGY GL WK+HV+ID +
Sbjct: 632 SRDWGFAGDYVEAMWLMLQQDKPDDYVVATDDSHTVEEFLKEAFGYVGLDWKEHVLIDPR 453
Query: 188 YFRPAEVDSLKGDATKARKVLKWKPKVGFQELVEMMVDHDIELAKKEKVLVDAGFRDPKQ 367
YFRPAEVD+L+GD++KAR++L W PKVGF++LV MMVD D+ELAK+EKVLVDAG+ D +Q
Sbjct: 452 YFRPAEVDNLRGDSSKARRLLGWAPKVGFKQLVAMMVDSDLELAKREKVLVDAGYIDSQQ 273
Query: 368 QP* 376
QP*
Sbjct: 272 QP* 264
Score = 155 bits (333), Expect = 3e-037
Identities = 73/120 (60%), Positives = 81/120 (67%)
Frame = -3 / +1
Query: 372 GCCLGSRNPASTRTFSFLAXXXXXXXXXXXXSWNPTLGFHLSTFLALVASPLRLSTSAGR 193
GCC S PASTRTFS LA NPT G H + LAL SPLRLSTSAG
Sbjct: 268 GCC*ESMYPASTRTFSLLANSKSESTIMATNCLNPTFGAHPRSLLALDESPLRLSTSAGL 447
Query: 192 KYFLSMTT*SFQLRPA*PKAACRNSSTVWDSSVATT*SPGCSCCSMSHIAST*SPANPQS 13
KY S++T SFQ P P A+ +NSSTVW SSVATT*S G SCC+++HIAST*SPANPQS
Sbjct: 448 KYLGSISTCSFQSNPTYPNASFKNSSTVWLSSVATT*SSGLSCCNINHIAST*SPANPQS 627
>gb|BX251594.1|BX251594 BX251594 Pinus pinaster differenciating xylem adult Pinus pinaster
cDNA clone PP051G03, mRNA sequence
Length = 706
Score = 234 bits (506), Expect = 4e-061
Identities = 91/124 (73%), Positives = 112/124 (90%)
Frame = +2 / +2
Query: 5 SARDWGFAGDYVEAMWLMLQQEQPGDYVVATEESHTVEEFLQAAFGYAGLSWKDHVVIDK 184
++RDWGFAGDYVEAMWLMLQQ++P DYVVAT++SHTVEEFL+ AFGY L WK+HV+ID
Sbjct: 236 ASRDWGFAGDYVEAMWLMLQQDKPDDYVVATDDSHTVEEFLKEAFGYVQLDWKEHVLIDP 415
Query: 185 KYFRPAEVDSLKGDATKARKVLKWKPKVGFQELVEMMVDHDIELAKKEKVLVDAGFRDPK 364
+YFRPAEVD+L+GD++KARK+L W PKVGF++LV MMVD D+ELAK+EKVLVDAG+ D +
Sbjct: 416 RYFRPAEVDNLRGDSSKARKLLGWAPKVGFKQLVAMMVDSDLELAKREKVLVDAGYIDSQ 595
Query: 365 QQP* 376
QQP*
Sbjct: 596 QQP* 607
Score = 155 bits (334), Expect = 2e-037
Identities = 74/120 (61%), Positives = 82/120 (68%)
Frame = -3 / -2
Query: 372 GCCLGSRNPASTRTFSFLAXXXXXXXXXXXXSWNPTLGFHLSTFLALVASPLRLSTSAGR 193
GCC S PASTRTFS LA NPT G H +FLAL SPLRLSTSAG
Sbjct: 603 GCC*ESMYPASTRTFSLLANSKSESTIMATNCLNPTFGAHPRSFLALDESPLRLSTSAGL 424
Query: 192 KYFLSMTT*SFQLRPA*PKAACRNSSTVWDSSVATT*SPGCSCCSMSHIAST*SPANPQS 13
KY S++T SFQ PKA+ +NSSTVW SSVATT*S G SCC+++HIAST*SPANPQS
Sbjct: 423 KYLGSISTCSFQSNCTYPKASFKNSSTVWLSSVATT*SSGLSCCNINHIAST*SPANPQS 244
>gb|DR049410.1|DR049410 RTBOR1_16_G01.b1_A029 Roots plus added boron Pinus taeda cDNA clone
RTBOR1_16_G01_A029 3', mRNA sequence
Length = 775
Score = 213 bits (459), Expect = 1e-054
Identities = 83/114 (72%), Positives = 103/114 (90%)
Frame = +2 / +1
Query: 35 YVEAMWLMLQQEQPGDYVVATEESHTVEEFLQAAFGYAGLSWKDHVVIDKKYFRPAEVDS 214
YVEAMWLMLQQ++P DYVVAT++SHTVEEFL+ AFGY GL WK+HV+ID +YFRPAEVD+
Sbjct: 1 YVEAMWLMLQQDKPDDYVVATDDSHTVEEFLKEAFGYVGLDWKEHVLIDPRYFRPAEVDN 180
Query: 215 LKGDATKARKVLKWKPKVGFQELVEMMVDHDIELAKKEKVLVDAGFRDPKQQP* 376
L+GD++KAR++L W PKVGF++LV MMVD D+ELAK+EKVLVDAG+ D +QQP*
Sbjct: 181 LRGDSSKARRLLGWAPKVGFKQLVAMMVDSDLELAKREKVLVDAGYIDSQQQP* 342
Score = 138 bits (295), Expect = 5e-032
Identities = 65/112 (58%), Positives = 73/112 (65%)
Frame = -3 / -3
Query: 372 GCCLGSRNPASTRTFSFLAXXXXXXXXXXXXSWNPTLGFHLSTFLALVASPLRLSTSAGR 193
GCC S PASTRTFS LA NPT G H + LAL SPLRLSTSAG
Sbjct: 338 GCC*ESMYPASTRTFSLLANSKSESTIMATNCLNPTFGAHPRSLLALDESPLRLSTSAGL 159
Query: 192 KYFLSMTT*SFQLRPA*PKAACRNSSTVWDSSVATT*SPGCSCCSMSHIAST 37
KY S++T SFQ P P A+ +NSSTVW SSVATT*S G SCC+++HIAST
Sbjct: 158 KYLGSISTCSFQSNPTYPNASFKNSSTVWLSSVATT*SSGLSCCNINHIAST 3
>gb|DR688225.1|DR688225 EST1078309 Normalized pine embryo library, Lib_D Pinus taeda cDNA
clone PWAC340 3' end, mRNA sequence
Length = 803
Score = 199 bits (430), Expect = 1e-050
Identities = 75/99 (75%), Positives = 92/99 (92%)
Frame = +2 / +3
Query: 5 SARDWGFAGDYVEAMWLMLQQEQPGDYVVATEESHTVEEFLQAAFGYAGLSWKDHVVIDK 184
++RDWGFAGDYVEAMWLM+QQ++P DYVVAT++SHTVEEFL+ AFGY GL+WKDHVVID
Sbjct: 507 ASRDWGFAGDYVEAMWLMVQQDKPDDYVVATDDSHTVEEFLKEAFGYVGLNWKDHVVIDP 686
Query: 185 KYFRPAEVDSLKGDATKARKVLKWKPKVGFQELVEMMVD 301
+YFRPAEVD+L+GD++KARK+L W PKVGF++LV MMVD
Sbjct: 687 RYFRPAEVDNLRGDSSKARKLLGWAPKVGFKQLVAMMVD 803
Score = 140 bits (301), Expect = 7e-033
Identities = 64/87 (73%), Positives = 70/87 (80%)
Frame = -3 / -2
Query: 273 NPTLGFHLSTFLALVASPLRLSTSAGRKYFLSMTT*SFQLRPA*PKAACRNSSTVWDSSV 94
NPT G H +FLAL SPLRLSTSAG KY S+TT*SFQ P PKA+ RNSSTVW SSV
Sbjct: 775 NPTFGAHPRSFLALDESPLRLSTSAGLKYLGSITT*SFQFNPTYPKASFRNSSTVWLSSV 596
Query: 93 ATT*SPGCSCCSMSHIAST*SPANPQS 13
ATT*S G SCC+++HIAST*SPANPQS
Sbjct: 595 ATT*SSGLSCCTINHIAST*SPANPQS 515
>gb|BE187175.1|BE187175 NXNV_160_B08_F Nsf Xylem Normal wood Vertical Pinus taeda cDNA
clone NXNV_160_B08 5' similar to Arabidopsis thaliana
sequence At5g66280 GDP-D-mannose 4,6-dehydratase; GMD1
(gb|AAF07199.1) see
http://mips.gsf.de/proj/thal/db/index.html, mRNA
sequence
Length = 539
Score = 176 bits (379), Expect = 1e-043
Identities = 66/97 (68%), Positives = 82/97 (84%)
Frame = +2 / +3
Query: 5 SARDWGFAGDYVEAMWLMLQQEQPGDYVVATEESHTVEEFLQAAFGYAGLSWKDHVVIDK 184
++RDWGFAGDYVEAMWLMLQQ++P DYVVAT++SHTVEEFL+ AFGY GL WK+HV+ID
Sbjct: 249 ASRDWGFAGDYVEAMWLMLQQDKPDDYVVATDDSHTVEEFLKEAFGYVGLDWKEHVLIDP 428
Query: 185 KYFRPAEVDSLKGDATKARKVLKWKPKVGFQELVEMM 295
+YF PAEVD+L D++ R+ L W PKVGF++L MM
Sbjct: 429 RYFXPAEVDNLXXDSSXXRRXLGWAPKVGFKQLXXMM 539
Score = 118 bits (252), Expect = 4e-026
Identities = 55/87 (63%), Positives = 62/87 (71%)
Frame = -3 / -2
Query: 273 NPTLGFHLSTFLALVASPLRLSTSAGRKYFLSMTT*SFQLRPA*PKAACRNSSTVWDSSV 94
NPT G H L S RLSTSAG KY S++T SFQ P P A+ +NSSTVW SSV
Sbjct: 517 NPTFGAHPRXLLXXDESXXRLSTSAGLKYLGSISTCSFQSNPTYPNASFKNSSTVWLSSV 338
Query: 93 ATT*SPGCSCCSMSHIAST*SPANPQS 13
ATT*S G SCC+++HIAST*SPANPQS
Sbjct: 337 ATT*SSGLSCCNINHIAST*SPANPQS 257
>gb|CF389075.1|CF389075 RTDR2_13_F12.b1_A021 Loblolly pine roots recovering from drought
DR2 Pinus taeda cDNA clone RTDR2_13_F12_A021 3', mRNA
sequence
Length = 522
Score = 107 bits (228), Expect = 8e-023
Identities = 40/50 (80%), Positives = 47/50 (94%)
Frame = +2 / -1
Query: 5 SARDWGFAGDYVEAMWLMLQQEQPGDYVVATEESHTVEEFLQAAFGYAGL 154
++RDWGFAGDYVEAMWLMLQQ++P DYVVAT++SHTVEEFL+ AFGY GL
Sbjct: 153 ASRDWGFAGDYVEAMWLMLQQDKPDDYVVATDDSHTVEEFLKEAFGYVGL 4
Database: Pinus_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:45 PM
Number of letters in database: 217,277,237
Number of sequences in database: 355,925
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 308,657,401
Number of Sequences: 355925
Number of extensions: 4018381
Number of successful extensions: 46694
Number of sequences better than 1.0e-020: 9
length of database: 72,425,745
effective HSP length: 51
effective length of database: 54,273,570
effective search space used: 8466676920
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)