TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2493961.2.1
(733 letters)
Database: Pinus_nucl_with_EST.fasta
355,925 sequences; 217,277,237 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|DR683247.1|DR683247 EST1073323 Normalized pine embryo li... 109 3e-043
gb|BQ634543.1|BQ634543 NXRV070_C05_F NXRV (Nsf Xylem Root w... 84 1e-030
gb|CF393446.1|CF393446 RTDR3_13_A12.b1_A022 Loblolly pine r... 97 2e-028
gb|DR119896.1|DR119896 RTMG1_26_F02.b1_A029 Roots minus mag... 109 3e-023
gb|DR025214.1|DR025214 STRS1_70_G04.b1_A034 Shoot tip pitch... 64 9e-023
gb|CF672808.1|CF672808 RTCNT1_74_C07.b1_A029 Root control P... 64 1e-022
gb|DR012583.1|DR012583 HEAT1_13_F08.g1_A029 Root at 37 C fo... 89 1e-021
>gb|DR683247.1|DR683247 EST1073323 Normalized pine embryo library, Lib_D Pinus taeda cDNA
clone PWAAF41, mRNA sequence
Length = 693
Score = 109 bits (232), Expect(2) = 3e-043
Identities = 42/65 (64%), Positives = 49/65 (75%)
Frame = -2 / -1
Query: 375 RRPFVTHFTGCQPCSGQRNEHYSGDSCDQGMRRALNFADDQVLRAYGFRHASSLSDDVQP 196
RRPFVTHFTGCQPCSG N+ Y G++C +GM RALNFADDQVL+ YGFRH + S V P
Sbjct: 378 RRPFVTHFTGCQPCSGDHNKIYKGENCWKGMERALNFADDQVLKNYGFRHDNLQSSHVNP 199
Query: 195 LPFDY 181
+ Y
Sbjct: 198 IQSFY 184
Score = 88.6 bits (187), Expect(2) = 3e-043
Identities = 31/52 (59%), Positives = 42/52 (80%)
Frame = -2 / -1
Query: 651 DDQSALVYMLLQEGSRWRDKVFLESGYYFEGYWMEIVGRLANMTERYEAMER 496
DDQSALVY+LL+E +W D++F+E YY GYW++IVG N+TE+YEAME+
Sbjct: 660 DDQSALVYLLLKEKDKWADRIFIEHKYYLNGYWLDIVGTYENITEKYEAMEK 505
>gb|BQ634543.1|BQ634543 NXRV070_C05_F NXRV (Nsf Xylem Root wood Vertical) Pinus taeda cDNA
clone NXRV070_C05 5' similar to Arabidopsis thaliana
sequence At4g37690 putative protein see
http://mips.gsf.de/proj/thal/db/index.html, mRNA
sequence
Length = 673
Score = 84.5 bits (178), Expect(2) = 1e-030
Identities = 28/55 (50%), Positives = 45/55 (81%)
Frame = -2 / +1
Query: 663 VSRVDDQSALVYMLLQEGSRWRDKVFLESGYYFEGYWMEIVGRLANMTERYEAME 499
V+ DDQSALVY++++E +W K+++E+ YYF+G+W++IVG N+++RYEAME
Sbjct: 241 VTESDDQSALVYLMIKEKKKWGGKIYIENEYYFQGFWLDIVGTFENISKRYEAME 405
Score = 71.2 bits (149), Expect(2) = 1e-030
Identities = 27/46 (58%), Positives = 30/46 (65%)
Frame = -2 / +1
Query: 375 RRPFVTHFTGCQPCSGQRNEHYSGDSCDQGMRRALNFADDQVLRAY 238
RRPFVTHF GCQPCSG N Y G++R+L FA DQVL Y
Sbjct: 511 RRPFVTHFAGCQPCSGNHNPIYXAXXXQNGLQRSLXFAXDQVLXIY 648
>gb|CF393446.1|CF393446 RTDR3_13_A12.b1_A022 Loblolly pine roots recovering from drought
DR3 Pinus taeda cDNA clone RTDR3_13_A12_A022 3', mRNA
sequence
Length = 572
Score = 96.8 bits (205), Expect(2) = 2e-028
Identities = 37/63 (58%), Positives = 43/63 (68%)
Frame = -2 / +2
Query: 375 RRPFVTHFTGCQPCSGQRNEHYSGDSCDQGMRRALNFADDQVLRAYGFRHASSLSDDVQP 196
RRPFVTHF GCQPCSG N Y + C G++R+L+FADDQVLR YGFR S V P
Sbjct: 194 RRPFVTHFAGCQPCSGNHNPIYKAEDCQNGLKRSLDFADDQVLRIYGFRRQQLQSVLVNP 373
Query: 195 LPF 187
+ F
Sbjct: 374 IHF 382
Score = 50.6 bits (104), Expect(2) = 2e-028
Identities = 16/29 (55%), Positives = 25/29 (86%)
Frame = -2 / +2
Query: 585 LESGYYFEGYWMEIVGRLANMTERYEAME 499
+E+ YYF+G+W++IVG N+++RYEAME
Sbjct: 2 IENEYYFQGFWLDIVGTFENISKRYEAME 88
>gb|DR119896.1|DR119896 RTMG1_26_F02.b1_A029 Roots minus magnesium Pinus taeda cDNA clone
RTMG1_26_F02_A029 3', mRNA sequence
Length = 599
Score = 109 bits (232), Expect = 3e-023
Identities = 42/65 (64%), Positives = 49/65 (75%)
Frame = -2 / +1
Query: 375 RRPFVTHFTGCQPCSGQRNEHYSGDSCDQGMRRALNFADDQVLRAYGFRHASSLSDDVQP 196
RRPFVTHFTGCQPCSG N+ Y G++C +GM RALNFADDQVL+ YGFRH + S V P
Sbjct: 100 RRPFVTHFTGCQPCSGDHNKIYKGENCWKGMERALNFADDQVLKNYGFRHDNLQSSHVNP 279
Query: 195 LPFDY 181
+ Y
Sbjct: 280 IQSFY 294
>gb|DR025214.1|DR025214 STRS1_70_G04.b1_A034 Shoot tip pitch canker susceptible Pinus taeda
cDNA clone STRS1_70_G04_A034 3', mRNA sequence
Length = 820
Score = 64.3 bits (134), Expect(3) = 9e-023
Identities = 22/47 (46%), Positives = 32/47 (68%)
Frame = -2 / +3
Query: 651 DDQSALVYMLLQEGSRWRDKVFLESGYYFEGYWMEIVGRLANMTERY 511
DDQSAL+Y+L+ + W DKVFLES Y+ G+W+ +V + +M Y
Sbjct: 186 DDQSALIYLLISQKKLWGDKVFLESSYFLHGFWVVVVDKYEDMIRTY 326
Score = 44.6 bits (91), Expect(3) = 9e-023
Identities = 16/31 (51%), Positives = 22/31 (70%)
Frame = -2 / +3
Query: 312 YSGDSCDQGMRRALNFADDQVLRAYGFRHAS 220
Y + C + M RA NFAD+Q+L+ YGF+H S
Sbjct: 405 YPVERCLKSMERAFNFADNQILQIYGFKHKS 497
Score = 35.4 bits (71), Expect(3) = 9e-023
Identities = 12/19 (63%), Positives = 14/19 (73%)
Frame = -2 / +3
Query: 390 GVHGWRRPFVTHFTGCQPC 334
G+ R PFVTHF GC+PC
Sbjct: 333 GLGDERWPFVTHFVGCKPC 389
>gb|CF672808.1|CF672808 RTCNT1_74_C07.b1_A029 Root control Pinus taeda cDNA clone
RTCNT1_74_C07_A029 3', mRNA sequence
Length = 679
Score = 64.3 bits (134), Expect(3) = 1e-022
Identities = 22/47 (46%), Positives = 32/47 (68%)
Frame = -2 / +3
Query: 651 DDQSALVYMLLQEGSRWRDKVFLESGYYFEGYWMEIVGRLANMTERY 511
DDQSAL+Y+L+ + W DKVFLES Y+ G+W+ +V + +M Y
Sbjct: 42 DDQSALIYLLISQKKLWGDKVFLESSYFLHGFWVVVVDKYEDMIRTY 182
Score = 44.6 bits (91), Expect(3) = 1e-022
Identities = 16/31 (51%), Positives = 22/31 (70%)
Frame = -2 / +3
Query: 312 YSGDSCDQGMRRALNFADDQVLRAYGFRHAS 220
Y + C + M RA NFAD+Q+L+ YGF+H S
Sbjct: 261 YPVERCLKSMERAFNFADNQILQIYGFKHKS 353
Score = 35.4 bits (71), Expect(3) = 1e-022
Identities = 12/19 (63%), Positives = 14/19 (73%)
Frame = -2 / +3
Query: 390 GVHGWRRPFVTHFTGCQPC 334
G+ R PFVTHF GC+PC
Sbjct: 189 GLGDERWPFVTHFVGCKPC 245
>gb|DR012583.1|DR012583 HEAT1_13_F08.g1_A029 Root at 37 C for 24 hr Pinus taeda cDNA clone
HEAT1_13_F08_A029 5', mRNA sequence
Length = 711
Score = 88.6 bits (187), Expect(2) = 1e-021
Identities = 34/60 (56%), Positives = 40/60 (66%)
Frame = -2 / -1
Query: 366 FVTHFTGCQPCSGQRNEHYSGDSCDQGMRRALNFADDQVLRAYGFRHASSLSDDVQPLPF 187
FVTHF GCQPCSG N Y + C G++R+L+FADDQVLR YGFR S V P+ F
Sbjct: 495 FVTHFAGCQPCSGNHNPIYKAEDCQNGLQRSLDFADDQVLRIYGFRRQQLQSVLVNPIHF 316
Score = 36.3 bits (73), Expect(2) = 1e-021
Identities = 13/32 (40%), Positives = 21/32 (65%)
Frame = -2 / -3
Query: 594 KVFLESGYYFEGYWMEIVGRLANMTERYEAME 499
+ L F+G+ ++IVG N+++RYEAME
Sbjct: 706 RFILRMSIIFQGFGLDIVGTFENISKRYEAME 611
Database: Pinus_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:45 PM
Number of letters in database: 217,277,237
Number of sequences in database: 355,925
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 322,679,157
Number of Sequences: 355925
Number of extensions: 4137172
Number of successful extensions: 38353
Number of sequences better than 1.0e-020: 9
length of database: 72,425,745
effective HSP length: 52
effective length of database: 53,917,645
effective search space used: 10298270195
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)